scholarly journals PERFORMANCE EVALUATION OF NUCLEATED RED BLOOD CELL (NRBC) COUNT USING A FULLY AUTOMATED HAEMATOLOGY ANALYZER VERSUS MANUAL COUNTING

2021 ◽  
Vol 71 (5) ◽  
pp. 1806-10
Author(s):  
Tanweer Ahmed ◽  
Asad Mahmood ◽  
Nasir Uddin ◽  
Helen Mary Robert ◽  
Muhammad Ashraf ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Linear Regression Analysis ◽  
Cell Counting ◽  
Nucleated Red Blood Cells ◽  
Manual Counting ◽  
Blood Cell Counting ◽  
Nucleated Red Blood Cell

Objective: To evaluate the performance of Nucleated RBC (NRBC) Count using a fully automated haematology analyzer versus manual counting. Study Design: Cross-Sectional Study. Place and Duration of Study: Department of Hematology, Armed Forces Institute of Pathology, from Sep 2019-Jun 2020. Methodology: Routine fresh whole blood samples were run on Sysmex XN-3000 automated haematology analyzer and 384 samples with results of ≥0.1% Nucleated red blood cells were included in this study. Manual NRBC counting was carried out twice on Leishman-stained peripheral blood smears from all 384 samples. Comparison between manual and automated nucleated red blood cell counting methods was statistically analyzed through linear regression analysis & coefficient correlation. The degree of agreement between two methods was analyzed through Bland-Altman plot. Finally, concordance between the two methods was also analyzed at 5 different ranges of nucleated red blood cells. Results: Linear regression analysis revealed a (r2) value of 0.97. Regression equation was calculated as XN = 0.76MC ± 1.28, with 95% limits of agreement between ± 40.42% and -24.47%. A mean bias of 7.97% was demonstrated through Bland-Altman plot. Concordance analysis revealed a concordance rate of 93.74% (360/384). Nucleated red blood cell counting between two methods were more concordant when nucleated red blood cell counts were <200%. Conclusion: Nucleated red blood cells counting by XN-3000 automated hematology analyzer is statistically comparable to manual nucleated red blood cell counting. We suggest that automated counting can be adopted in routine hematology laboratory as a replacement of manual NRBC counting.

Fetal nucleated red blood cell analysis for non-invasive prenatal diagnostics using a nanostructure microchip

2017 ◽  
Vol 5 (2) ◽  
pp. 226-235 ◽  
Author(s):  
Zhaobo He ◽  
Feng Guo ◽  
Chun Feng ◽  
Bo Cai ◽  
James P. Lata ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Prenatal Diagnostics ◽  
Cell Analysis ◽  
Non Invasive ◽  
Nucleated Red Blood Cells ◽  
Nucleated Red Blood Cell

A microchip is applied to isolate andin situanalyze fetal nucleated red blood cells for non-invasive prenatal diagnostics.

CLINICAL STUDIES OF THE EFFECTS OF 3,5,5, TRIMETHYLOXAZOLIDINE-2-4-DIONE (TRIDIONE®) ON THE HEMATOPOIETIC SYSTEM, LIVER AND KIDNEY

PEDIATRICS ◽  
1950 ◽  
Vol 5 (4) ◽  
pp. 695-707
Author(s):  
ERIC DENHOFF ◽  
MAURICE W. LAUFER
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Blood Platelet ◽  
Clot Retraction ◽  
Nucleated Red Blood Cells ◽  
Complete Blood Counts ◽  
Nucleated Red Blood Cell

Six children receiving tridione® showed : 1. A constant syndrome of : a decline in bone marrow megakaryocyte and nucleated red blood cell elements; a low peripheral blood platelet count; and prolonged clot retraction time. 2. An occasional association of: peripheral leukopenia, toxic signs in the red blood cells and lymphocytes, and an abnormal cephalin flocculation response. In one case the bone marrow megakaryocyte response occurred alone and was temporary; in another it was temporary but associated with a persistent decline in marrow nucleated red blood cells; and in a third case both the megakaryocyte and nucleated red blood cell depression were only temporary, despite continuation of the medication. Withdrawal of medication caused a return to normal values, most rapid and marked in the bone marrow. Tests for clot retraction time and platelet counts at regular intervals in addition to complete blood counts seem indicated in all patients receiving tridione®. When available, bone marrow studies, especially of megakaryocytes, would also be of value.

Real-time Red Blood Cell Counting and Osmolarity Analysis using a Photoacoustic-based Microfluidic System

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Wenxiu Zhao ◽  
Haibo Yu ◽  
Yangdong Wen ◽  
Hao Luo ◽  
Boliang Jia ◽  
...  
Keyword(s):  
Blood Cell ◽  
Physical Properties ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Diagnostic Procedure ◽  
Microfluidic System ◽  
Cell Counting ◽  
Blood Samples ◽  
System P ◽  
Blood Cell Counting

Counting the number of red blood cells (RBCs) in blood samples is a common clinical diagnostic procedure, but conventional methods are unable to provide the size and other physical properties...

Microfluidic electrical impedance assessment of red blood cell mediated microvascular occlusion

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yuncheng Man ◽  
Debnath Maji ◽  
Ran An ◽  
Sanjay Ahuja ◽  
Jane A Little ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Electrical Impedance ◽  
Cell Disease ◽  
Blood Disorders

Alterations in the deformability of red blood cells (RBCs), occurring in hemolytic blood disorders such as sickle cell disease (SCD), contributes to vaso-occlusion and disease pathophysiology. However, there are few...

Adaptive and automatic red blood cell counting method based on microscopic hyperspectral imaging technology

2017 ◽  
Vol 19 (12) ◽  
pp. 124014 ◽  
Author(s):  
Xi Liu ◽  
Mei Zhou ◽  
Song Qiu ◽  
Li Sun ◽  
Hongying Liu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Hyperspectral Imaging ◽  
Red Blood Cell ◽  
Cell Counting ◽  
Counting Method ◽  
Imaging Technology ◽  
Blood Cell Counting

Permeation of the luminal capillary glycocalyx is determined by hyaluronan

1999 ◽  
Vol 277 (2) ◽  
pp. H508-H514 ◽  
Author(s):  
Charmaine B. S. Henry ◽  
Brian R. Duling
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Enzyme Treatment ◽  
Apical Surface ◽  
Bright Field ◽  
Endothelial Surface ◽  
The Difference

The endothelial cell glycocalyx influences blood flow and presents a selective barrier to movement of macromolecules from plasma to the endothelial surface. In the hamster cremaster microcirculation, FITC-labeled Dextran 70 and larger molecules are excluded from a region extending almost 0.5 μm from the endothelial surface into the lumen. Red blood cells under normal flow conditions are excluded from a region extending even farther into the lumen. Examination of cultured endothelial cells has shown that the glycocalyx contains hyaluronan, a glycosaminoglycan which is known to create matrices with molecular sieving properties. To test the hypothesis that hyaluronan might be involved in establishing the permeation properties of the apical surface glycocalyx in vivo, hamster microvessels in the cremaster muscle were visualized using video microscopy. After infusion of one of several FITC-dextrans (70, 145, 580, and 2,000 kDa) via a femoral cannula, microvessels were observed with bright-field and fluorescence microscopy to obtain estimates of the anatomic diameters and the widths of fluorescent dextran columns and of red blood cell columns (means ± SE). The widths of the red blood cell and dextran exclusion zones were calculated as one-half the difference between the bright-field anatomic diameter and the width of the red blood cell column or dextran column. After 1 h of treatment with active Streptomyces hyaluronidase, there was a significant increase in access of 70- and 145-kDa FITC-dextrans to the space bounded by the apical glycocalyx, but no increase in access of the red blood cells or in the anatomic diameter in capillaries, arterioles, and venules. Hyaluronidase had no effect on access of FITC-Dextrans 580 and 2,000. Infusion of a mixture of hyaluronan and chondroitin sulfate after enzyme treatment reconstituted the glycocalyx, although treatment with either molecule separately had no effect. These results suggest that cell surface hyaluronan plays a role in regulating or establishing permeation of the apical glycocalyx to macromolecules. This finding and our prior observations suggest that hyaluronan and other glycoconjugates are required for assembly of the matrix on the endothelial surface. We hypothesize that hyaluronidase creates a more open matrix, enabling smaller dextran molecules to penetrate deeper into the glycocalyx.

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