Quick and simultaneous determination of caffeine and taurine in beverages using UPLC-ESI-MS

A rapid UPLC-ESI-MS method was developed for simultaneous determination of caffeine and taurine in beverages (energy drinks and soft drinks). The molecular ions of caffeine and taurine were identified in single ion recording mode at m/z 194.98 and 125.86, respectively. The mass spectrometer parameters were optimized as: capillary voltage 3.0 kV, cone voltage 35 V, extractor 3 V, RF Lens 0.1 V, source temperature 150 °C, desolvation temperature 350 °C, nitrogen 600 L/h, LMR1 7.9, HMR1 15.2, IE1 0.30. The mobile phase comprising methanol (0.1% formic acid) (A) and water (5 mM ammonium acetate) (B) was used in gradient mode. The mobile phase components A and B were pumped in 80:20 (v:v) ratio from 0-0.44 min, and then 100% of component A was pumped between 0.45-0.68 min, and at 0.69 min the composition was returned to 80:20 (v:v) ratio of A and B till 2.0 min. Caffeine and taurine were eluted at 0.46 and 0.43 min, respectively. The samples of energy drinks and soft drinks were diluted in a solvent system comprising methanol and water in 80:20 (v:v) ratio. Our investigations showed that soft drinks SD1 and SD2 have 88.8±4.2% and 110.7±3.6% (w:w) caffeine of their labeled claim. The caffeine content in energy drink brands ED1, ED2, ED3, and ED4 was 76.9±2.5, 65.6±3.4, 88.1±12.6, and 89.1±2.8% (w:w) of labeled claims, respectively. While taurine content in ED1, ED2, ED3, and ED4 was 86.5±8.4, 81.3±27.5, 101.9±4.8, and 97.1±0.3% (w:w) of labeled claim, respectively.

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Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks

International Journal of Analytical Chemistry ◽

10.1155/2016/2879406 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Bürge Aşçı ◽

Şule Dinç Zor ◽

Özlem Aksu Dönmez

Keyword(s):

Simultaneous Determination ◽

Flow Rate ◽

Mobile Phase ◽

High Performance ◽

Food Additives ◽

Hplc Method ◽

Soft Drinks ◽

Phase Ratio ◽

Potassium Sorbate

Box-Behnken design was applied to optimize high performance liquid chromatography (HPLC) conditions for the simultaneous determination of potassium sorbate, sodium benzoate, carmoisine, allura red, ponceau 4R, and caffeine in commercial soft drinks. The experimental variables chosen were pH (6.0–7.0), flow rate (1.0–1.4 mL/min), and mobile phase ratio (85–95% acetate buffer). Resolution values of all peak pairs were used as a response. Stationary phase was Inertsil OctaDecylSilane- (ODS-) 3V reverse phase column (250 × 4.6 mm, 5 μm) dimensions. The detection was performed at 230 nm. Optimal values were found 6.0 pH, 1.0 mL/min flow rate, and 95% mobile phase ratio for the method which was validated by calculating the linearity (r2>0.9962), accuracy (recoveries ≥ 95.75%), precision (intraday variation ≤ 1.923%, interday variation ≤ 1.950%), limits of detection (LODs), and limits of quantification (LOQs) parameters. LODs and LOQs for analytes were in the range of 0.10–0.19 μg/mL and 0.33–0.63 μg/mL, respectively. The proposed method was applied successfully for the simultaneous determination of the mixtures of five food additives and caffeine in soft drinks.

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Rapid High-Performance Liquide Chromatographic Method for Quantitative Determination of Caffeine in Different Soft and Energy Drinks Available in Bangladesh

Current Research in Nutrition and Food Science Journal ◽

10.12944/crnfsj.9.3.33 ◽

2021 ◽

Vol 9 (3) ◽

pp. 1081-1089

Author(s):

Juthi Mirza ◽

Masuda Sultana ◽

Md. Esrafil ◽

Shamoli Akter ◽

Md. Jahangir Alam ◽

...

Keyword(s):

High Performance ◽

Food Additives ◽

Reference Value ◽

Energy Drinks ◽

Energy Drink ◽

Hplc Method ◽

Soft Drinks ◽

Caffeine Content ◽

And Control

Caffeine is one of the commonly used food additives, which has unique flavor characteristics and bitter taste and used in soft drinks as flavor enhancer. An experimental study was designed to determine the concentration of caffeine in different brands of soft drinks and energy drinks available in Bangladesh by using HPLC. For chromatographic analysis, A Luna 5 C18 (2) 100A column (250×4.6 mm) was used at 37°C temperature at the wavelength of 272nm. Chromatographic separation was determined using buffer of sodium acetate and acetic acid with acetonitrile at a ratio of 80:20 (pH=4.0; flow rate of 1.0 ml/min). The results of this study showed that caffeine content in soft drinks ranged from 19.63 to 101.73 mg/100ml and highest concentration of caffeine found in brand 3 samples while lowest concentration found in brand 2 samples. Significantly higher concentration of caffeine (p<0.05) found in six soft drinks sample when compared to BSTI and FDA reference value except brand 2 sample (p>0.05). Quantification of caffeine in different brands of energy drink sample revealed that, four brand sample contained caffeine; among them brand 3 sample showed the highest levels of caffeine 295.86 mg/100ml and lowest amount found in brand 1 sample (101.74 mg/100ml). Concentration of Caffeine in soft and energy drinks exceeded the national and international standard recommended range hence this proposed HPLC method can be used for routine determination and control of caffeine content in different drinks.

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Determination of Caffeine in Beverages Found in Bangladeshi Market by High Performance Liquid Chromatography (HPLC)

Oriental Journal Of Chemistry ◽

10.13005/ojc/370320 ◽

2021 ◽

Vol 37 (3) ◽

pp. 663-666

Author(s):

G. M. M. Anwarul Hasan ◽

Anuj Kumer Das

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Mobile Phase ◽

High Performance ◽

Energy Drinks ◽

Soft Drinks ◽

Caffeine Content

Caffeine, a phycostimulant is present in several foods and drinks.In the present study, beverages of different brands in Bangladeshi market were analyzed for caffeine by high-performance liquid chromatography (HPLC) using methanol-water (40:60, v/v) as mobile phase. Caffeine content ranged from 16.33 -19.33 mg/can in soft drinks and 45.66-47.33 mg/can in energy drinks respectively. These data indicated that the levels of caffeine in Bangladeshi soft drinks and energy drinks are within the ranges reported from similar products in other countries.

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A Green Liquid Chromatographic Method For The Simultaneous Determination of Chlorpheniramine Maleate, Pseudoephedrine Hydrochloride and Propyphenazone

Current Analytical Chemistry ◽

10.2174/1573411014666180806155002 ◽

2019 ◽

Vol 15 (6) ◽

pp. 635-641

Author(s):

Nadia M. Mostafa ◽

Ghada M. Elsayed ◽

Nagiba Y. Hassan ◽

Dina A. El Mously

Keyword(s):

Simultaneous Determination ◽

Mobile Phase ◽

Dosage Form ◽

Chromatographic Method ◽

Green Analytical Chemistry ◽

Chlorpheniramine Maleate ◽

Accuracy And Precision ◽

Liquid Chromatographic Method ◽

Liquid Chromatographic

Background:The concept of green analytical chemistry prevails due to the growing environmental pollution.Objective:Our attempts are to develop simple and eco-friendly method which is non-harmful to the environment by producing minimal waste. In this context, a green liquid chromatographic method was applied for the simultaneous determination of chlorpheniramine maleate, pseudoephedrine hydrochloride and propyphenazone in their combined dosage form.Methods:Separation was carried out using X select HSS RP C18 analytical column (250 × 4.6 mm, 5μm) using methanol - 0.02 M phosphate buffer pH 3 - triethylamine (60:40: 0.1, by volume) as a mobile phase. The separated peaks were detected at 215 nm at a flow rate 1.0 mL/min.Results:Quantification was done over the concentration ranges of 1-25 µg/mL for chlorpheniramine maleate, 5-35 µg/mL for pseudoephedrine hydrochloride and 10-120 µg/mL for propyphenazone. The suggested method was validated with regard to linearity, accuracy and precision according to the International Conference on Harmonization guidelines with good results.Conclusion:It could be used as a safer alternative for routine analysis of the mentioned drugs in quality control laboratories.

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Reverse phase-liquid chromatography assisted protocol for simultaneous determination of lamivudine and tenofovir disoproxil fumarate in combined medication used to control HIV infection: an investigative approach

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-021-00233-3 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Vaibhav S. Adhao ◽

Suraj R. Chaudhari ◽

Jaya P. Ambhore ◽

Sunil Sangolkar ◽

Raju R. Thenge ◽

...

Keyword(s):

Liquid Chromatography ◽

Hiv Infection ◽

Simultaneous Determination ◽

Tenofovir Disoproxil Fumarate ◽

Solvent System ◽

Array Detector ◽

Technical Requirements ◽

Relative Standard ◽

Limit Of Quantitation

Abstract Background Human immunodeficiency virus (HIV) causes severe life-threatening condition, i.e., AIDS. HIV destabilises an individual’s ability to prevent infection. Therefore, the combine medication lamivudine (LVD) and tenofovir disoproxil fumarate (TDF) are prescribed to suppress the amount of HIV infection in individual’s body; thus, the individual’s immune system could function properly. Consequently, the objective of present research work was to investigate robust and sensitive liquid chromatography avenue for simultaneous determination of lamivudine and tenofovir disoproxil fumarate in pure material and combined dosage form. Results The reversed-phase chromatographic separation has been performed through Hypersil BDS C18 column using solvent system composed of 10 mM potassium dihydrogen phosphate (pH 4.0): acetonitrile (60:40% v/v). The determination was executed at 30 oC at 1 mL/min rate for flow of solvent system through column. The eluents of column were monitored at 265 nm using Photodiode Array detector has revealed admirable retention times, i.e., 4.67 and 8.78 min for both drugs, respectively. The calibration curve demonstrated excellent linearity in the range of 10–50 μg/mL for lamivudine and tenofovir disoproxil fumarate with better determination coefficients was more than (r2 0.999). Conclusion The estimable method was effectively validated with respect to accuracy, precision, sensitive (limit of detection and limit of quantitation), robustness, ruggedness, and for selectivity and specificity. The value less than 2 for percentage relative standard deviation for accuracy, precision, robustness, and ruggedness satisfying the acceptance criteria as per procedure of International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use.

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Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

E-Journal of Chemistry ◽

10.1155/2011/360431 ◽

2011 ◽

Vol 8 (1) ◽

pp. 340-346 ◽

Cited By ~ 5

Author(s):

Rajesh M. Kashid ◽

Santosh G. Singh ◽

Shrawan Singh

Keyword(s):

High Performance Liquid Chromatography ◽

Simultaneous Determination ◽

Mobile Phase ◽

High Performance ◽

Reversed Phase ◽

Hplc Method ◽

Reversed Phase Hplc ◽

Methyl Paraben ◽

Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

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