scholarly journals Biotherapic produced from serum of refractory to Trypanosoma cruzi animal increases survival in mice infected with the protozoan.

Author(s):  
Erika Cristina Ferreira ◽  
Larissa Ciupa ◽  
Denise Lessa Aleixo ◽  
Silvana Marques De Araújo

Trypanosoma cruzi infection affects approximately eight million people from the Southern United States to Southern Argentina1, with 1.5 million of them in Brazil. The lack of an effective medication for the causative treatment of this infection arouses the interest of many researchers. Considering the data obtained by our team regarding the effects of biotherapic in murine infection with T. cruzi2-7, the relationship of the effects of biotherapic with the biological material used and the species from which this material is collected, the resistance or susceptibility of this species against the pathology to be treated2-7, and the refractoriness of birds8 to T. cruzi, in this study we evaluated the effects of ultradiluted serum of Gallus gallus domesticus on the treatment of mice infected with Trypanosoma cruzi. Methodology: In a blind, controlled and randomized assay, 21 male Swiss mice, eight weeks-old, were allocated into three groups: CI: infected animals without treatment(n=7); Alcohol13cH: infected and treated with alcohol 13cH animals(n=7); Serum13cH: infected and treated with serum of Gallus gallus domesticus 13cH animals(n = 7). The Infected animals were inoculated with 1400 blood trypomastigotes of T. cruzi-Y strain. Medications were prepared from uninfected serum of Gallus gallus domesticus according Brazilian Pharmacopoeia Homeopathy9 and administered two days before infection and on days 2, 5 and 8 after infection. The medication was diluted in the drinking water at concentration of 1% and offered ad libitum overnight. The groups were evaluated regarding survival period up to 161 days (endpoint). The project was submitted to the Ethics Committee CEUA Nº 2401220716 . Results: Data are presented in Figure 1. The animals that received treatment with Gallus gallus domesticus serum 13cH(Serum 13cH) had higher survival rate(p

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 777
Author(s):  
Korakot Nganvongpanit ◽  
Piyatida Kaewkumpai ◽  
Varankpicha Kochagul ◽  
Kidsadagon Pringproa ◽  
Veerasak Punyapornwithaya ◽  
...  

The black-bone chicken (Gallus gallus domesticus) is a breed of chicken that is commonly found in Thailand. This breed is known for having a number of black colored organs. Consumers have been notably attracted to the black-bone chicken breed for the characteristic darkness that is observed in many of its organs. However, the degree of darkness in all organs of the black-bone chicken is still in question. Importantly, there have not yet been any published reports on the distribution of melanin pigment in the organs of the black-bone chicken. This research study aims to examine the distribution of the melanin pigment in 33 organs of the Thai black-bone chicken. Ten black-bone chickens (five male, five female) were included in this study. Thirty-two organs including the brain, spinal cord, sciatic nerve, larynx, trachea, syrinx, lungs, heart, pericardium, aorta, brachial vein, kidney, cloaca, oviduct, testis, gastrocnemius muscle, femur, tongue, esophagus, crop, proventriculus, gizzard, duodenum, jejunum, ileum, cecum, pancreas, liver, gall bladder, omentum, abdominal fat, spleen, and skin were examined in this study. Histological sections taken from tissue samples of each of these organs were studied. The findings revealed that the presence of the melanin pigment was not significantly different (p > 0.005) between male and female specimens. Notably, the liver was the only organ in which the melanin pigment had not accumulated. Consequently, there was not a uniform pattern of melanin pigment accumulation throughout the organs of the chickens. The melanin pigment was present in all of the tissue layers of most organs, while the melanin pigment was found in only specific layers of some of the organs. In conclusion, the distribution of melanin pigmentation in the organs of each of the animals in this study was found to be different. However, in some tissue samples, such as those obtained from the liver, no accumulation of the melanin pigment was observed.


Author(s):  
Webster Leonardo Guimarães da Costa ◽  
Isa Marianny Ferreira Nascimento Barbosa ◽  
Débora Pereira Gomes do Prado ◽  
Natália Domann ◽  
Hanstter Hallison Alves Rezende

2020 ◽  
Vol 23 (4) ◽  
pp. 769-780 ◽  
Author(s):  
Vitor Hugo Bessa Ferreira ◽  
Maxence Barbarat ◽  
Flore Lormant ◽  
Karine Germain ◽  
Mathilde Brachet ◽  
...  

1964 ◽  
Vol 23 (1) ◽  
pp. 63-78 ◽  
Author(s):  
James R. Coleman ◽  
Montrose J. Moses

The indium trichloride method of Watson and Aldridge (38) for staining nucleic acids for electron microscopy was employed to study the relationship of DNA to the structure of the synaptinemal complex in meiotic prophase chromosomes of the domestic rooster. The selectivity of the method was demonstrated in untreated and DNase-digested testis material by comparing the distribution of indium staining in the electron microscope to Feulgen staining and ultraviolet absorption in thicker sections seen with the light microscope. Following staining by indium, DNA was found mainly in the microfibril component of the synaptinemal complex. When DNA was known to have been removed from aldehyde-fixed material by digestion with DNase, indium stainability was also lost. However, staining of the digested material with non-selective heavy metal techniques demonstrated the presence of material other than DNA in the microfibrils and showed that little alteration in appearance of the chromosome resulted from DNA removal. The two dense lateral axial elements of the synaptinemal complex, but not the central one to any extent, also contained DNA, together with non-DNA material.


2013 ◽  
Vol 195 (1-2) ◽  
pp. 203-204
Author(s):  
Caroline Gracielle Torres Ferreira ◽  
Cláudio Mafra ◽  
Ana Carla Diógenes Suassuna Bezerra ◽  
Otávio Valério de Carvalho ◽  
Abelardo Silva ◽  
...  

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