scholarly journals Case study of a Hungarian breeding program using imported Booroola rams

2004 ◽  
Vol 47 (4) ◽  
pp. 359-366
Author(s):  
M. Árnyasi ◽  
A. Zsolnai ◽  
I. Komlósi ◽  
L. Fésüs ◽  
A. Jávor
Keyword(s):  
Major Gene ◽  
Ovulation Rate ◽  
Dna Testing ◽  
Bone Morphogenetic Protein Receptor ◽  
Long Period ◽  
Protein Receptor ◽  
Morphogenetic Protein ◽  
Pcr Rflp ◽  
Phenotype Measurement

Abstract. The first major gene for prolificacy identified in sheep was the Booroola (FecB) gene. Since the recognition of its existence, the Booroola Merino has spread all over the world. In Hungary, a new breed – called Hungarian Prolific Merino – had been established based on the crossing of Hungarian Merino ewes and Booroola Merino rams, and was acknowledged in 1992. The only way to determine the FecB genotypes has been the measurement of the ovulation rate over a long period. In 2001, the Booroola mutation was identified. Mutation on the bone morphogenetic protein receptor – 1B gene was found to be associated with the increased ovulation rate in the Booroola Merino ewes. 138 ewes and 46 rams in the Hungarian Prolific Merino population were tested for this mutation by PCR-RFLP and their FecB genotypes were determined. One copy of the FecB allele increased (P < 0,05) the ovulation rate by 0.89 ova and two copies increased by an average of 2.27 ova. Effectiveness of the FecB genotype estimation based on phenotype measurement was also compared to the results of direct DNA testing, and was found to have up to 80% accuracy.

scholarly journals Oocytes in sheep homozygous for a mutation in bone morphogenetic protein receptor 1B express lower mRNA levels of bone morphogenetic protein 15 but not growth differentiation factor 9

Reproduction ◽  
2011 ◽  
Vol 142 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Janet L Crawford ◽  
Derek A Heath ◽  
Karen L Reader ◽  
Laurel D Quirke ◽  
Norma L Hudson ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Cumulus Cell ◽  
Ovulation Rate ◽  
Mrna Levels ◽  
Camp Production ◽  
Expression Levels ◽  
Bone Morphogenetic Protein Receptor ◽  
Protein Receptor ◽  
Morphogenetic Protein ◽  
Validation Experiment

The aim of this study was to test the hypothesis that the high ovulation rate in ewes (BB) homozygous for a mutation in the bone morphogenetic protein receptor type 1B (BMPR1B) gene is linked to lower BMP15 and/or GDF9 mRNA in oocytes compared with those in wild-type (++) ewes. Cumulus cell–oocyte complexes (COC) and granulosa cells (GC) were recovered from ≥1 mm diameter follicles of BB and ++ ewes during a prostaglandin-induced follicular phase. Expression levels of GDF9 and BMP15 were measured by multiplex qPCR from individual COC. The gonadotropin-induced cAMP responses of the GC from each non-atretic follicle were measured following treatment with FSH or human chorionic gonadotropin. In a separate validation experiment, GDF9 and BMP15 expression was present only in oocytes and not in cumulus cells. There was no effect of follicular diameter on oocyte-derived GDF9 or BMP15 mRNA levels. The mean expression levels of BMP15, but not GDF9, were significantly lower in all non-atretic follicles, including the subsets containing either FSH- or LH-responsive GC in BB, compared with ++, ewes. No genotype effects were noted for FSH-induced cAMP production by GC either with respect to dose of, or number of follicles responding to, FSH. However, ovaries from BB ewes contained significantly more follicles responsive to LH, with respect to cAMP production in GC. We propose that these findings are consistent with the hypothesis that the higher ovulation rate in BB sheep is due, at least in part, to lower oocyte-derived BMP15 mRNA levels together with the earlier onset of LH-responsiveness in GC.

Bone morphogenetic protein receptor IB as a candidate gene for prolificacy in Small Tail Han and Hu ewes

2005 ◽  
Vol 2 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Yan Ya-Dong ◽  
Chu Ming-Xing ◽  
Zeng Yong-Qing ◽  
Fang Li ◽  
Ye Su-Cheng ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Bone Morphogenetic Protein ◽  
Major Gene ◽  
Molecular Genetic ◽  
Single Strand Conformation Polymorphism ◽  
Single Nucleotide ◽  
Bone Morphogenetic Protein Receptor ◽  
Sheep Breeds ◽  
Protein Receptor ◽  
Morphogenetic Protein

AbstractThe bone morphogenetic protein receptor IB (BMPR-IB) gene, which controls the fecundity of Booroola Merino ewes, was studied as a candidate gene for the prolificacy of Small Tail Han and Hu ewes. A single nucleotide polymorphism of the BMPR-IB gene was detected in both high (Small Tail Han and Hu) and low (Suffolk and Dorset) fecundity sheep breeds by polymerase chain reaction–single-strand conformation polymorphism (PCR-SSCP) analysis. The results indicated the presence of the same mutation (A746G) of the BMPR-IB gene in both Small Tail Han and Hu ewes and in Booroola Merino ewes, but not in both Suffolk and Dorset ewes. In Small Tail Han ewes, frequencies of BB, B+ and ++ genotypes were 0.524, 0.383 and 0.093, respectively. In Hu ewes, these frequencies were 0.882, 0.118 and 0.000. The BMPR-IB genotype distributions were significantly different (P<0.001) among high- and low-fecundity sheep breeds. Small Tail Han ewes with genotype BB had 0.92 (P<0.01) and 1.02 (P<0.01) lambs more than those with genotype ++ in the first and second parity, respectively. These results demonstrated that the BMPR-IB gene is a major gene affecting the prolificacy in both Small Tail Han and Hu ewes, and could be used as a molecular genetic marker to select the litter size in sheep.

scholarly journals Investigating the Polymorphism of Bone Morphogenetic Protein Receptor-1B (BMPR1B) Gene in Markhoz Goat Breed

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1582
Author(s):  
Sheila Pourali Dogaheh ◽  
Seyed Ziaeddin Mirhoseini ◽  
Vincenzo Tufarelli ◽  
Navid Ghavi Hossein-Zadeh ◽  
Sajad Badbarin ◽  
...  
Keyword(s):  
Genetic Resource ◽  
Economic Value ◽  
Reproductive Traits ◽  
Major Effect ◽  
Autosomal Gene ◽  
Chromosome 6 ◽  
Bone Morphogenetic Protein Receptor ◽  
Protein Receptor ◽  
Morphogenetic Protein ◽  
Pcr Rflp

Reproductive traits in livestock species are genetically controlled by the action of single genes with a major effect, commonly known as fecundity genes. One of the genes involved in controlling prolificacy is BMPR1B (FecB), a dominant autosomal gene located in chromosome 6 responsible for the fecundity and twinning rate in sheep and goat species. Markhoz goat is a valuable Iranian genetic resource endangered by extinction. Increasing the genetic variability and reproductive performances of Markhoz goat could preserve and enhance its economic value. This study was carried out to detect possible polymorphisms in BMPR1B gene in a sample of 100 Markhoz goats from Iran. DNA samples were screened by PCR–RFLP to assess the presence of the previously reported FecB mutation. Finally, the amplicons from seven goats out of the 100 samples were sequenced. The results showed that all the analyzed individuals did not carry the previously reported FecB mutant allele. However, our findings revealed two novel possible mutations in exon 8 of BMPR1B gene (775A > G and 777G > A) that need further investigations.

Association Study of Fecundity Gene BMPR1B with Prolificacy in Surti Goats under Farm and Field Condition

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
N. S. Dangar ◽  
G. M. Pandya ◽  
U. V. Ramani ◽  
Y. D. Padheriya ◽  
T. Sangma ◽  
...  
Keyword(s):  
Litter Size ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Pcr Primers ◽  
Dual Purpose ◽  
Protein Receptor ◽  
Morphogenetic Protein ◽  
Pcr Rflp ◽  
Base Size ◽  
Mutation Information

The Surti is a dual purpose goat breed of Gujarat. The bone morphogenetic protein receptor type 1B (BMPR1B) gene of transforming growth factor beta (TGF-β) superfamily ligands is playing a role in ovulation as well as litter size. Mutation in Exon-6 region of BMPR1B gene with base size 190 bp reported increasing litter size. Based on the known mutation information in goat and sheep, PCR primers were designed to screen polymorphism in total 100 Surti goats, 50 Surti goats from University Farm, Navsari and 50 Surti goats from field units of Southern part of Gujarat. During PCR-RFLP study no polymorphic sites were found for Exon-6 region of BMPR1B on Surti goats. Moreover, the twinning rate was 10% in first parity and higher in subsequent second (62.5%) and third (76.8%) parties.

scholarly journals Identification of a Lysosomal Pathway Regulating Degradation of the Bone Morphogenetic Protein Receptor Type II

2010 ◽  
Vol 285 (48) ◽  
pp. 37641-37649 ◽  
Author(s):  
Hannah J. Durrington ◽  
Paul D. Upton ◽  
Simon Hoer ◽  
Jessica Boname ◽  
Benjamin J. Dunmore ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Receptor Type ◽  
Type Ii ◽  
Bone Morphogenetic Protein Receptor ◽  
Protein Receptor ◽  
Morphogenetic Protein
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