scholarly journals Diversity and abundance of <i>n</i>-alkane degrading bacteria in the near surface soils of a Chinese onshore oil and gas field

2012 ◽  
Vol 9 (10) ◽  
pp. 14867-14887
Author(s):  
K. Xu ◽  
Y. Tang ◽  
C. Ren ◽  
K. Zhao ◽  
Y. Sun

Abstract. Alkane degrading bacteria have long been used as an important biological indicator for oil and gas prospecting, but their ecological characteristics in hydrocarbon microseep habitats are still poorly understood. In this study, the diversity and abundance of n-alkane degrading bacterial community in the near surface soils of a Chinese onshore oil and gas field were investigated using molecular techniques. Terminal restriction fragment length polymorphism (T-RFLP) analyses in combination with cloning and sequencing of alkB genes revealed that trace amount of volatile hydrocarbons migrated from oil and gas reservoirs caused a shift of the n-alkane degrading bacterial community from Gram-positive bacteria (Mycobacterium and Rhodococcus) to Gram-negative genotypes (Alcanivorax and Acinetobacter). Real-time PCR results furthermore showed that the abundance of alkB genes increased substantially in the surface soils underlying oil and gas reservoirs even though only low or undetectable concentrations of hydrocarbons were measured in these soils due to efficient microbial degradation. Our findings broadened the knowledge on the ecological characteristics of alkane degrading community in hydrocarbon microseeps and may provide a new approach for microbial prospecting for oil and gas (MPOG).

2013 ◽  
Vol 10 (3) ◽  
pp. 2041-2048 ◽  
Author(s):  
K. Xu ◽  
Y. Tang ◽  
C. Ren ◽  
K. Zhao ◽  
Y. Sun

Abstract. Alkane-degrading bacteria have long been used as an important biological indicator for oil and gas prospecting, but their ecological characteristics in hydrocarbon microseep habitats are still poorly understood. In this study, the diversity and abundance of n-alkane-degrading bacterial community in the near-surface soils of a Chinese onshore oil and gas field were investigated using molecular techniques. Terminal restriction fragment length polymorphism (T-RFLP) analyses in combination with cloning and sequencing of alkB genes revealed that Gram-negative genotypes (Alcanivorax and Acinetobacter) dominated n-alkane-degrading bacterial communities in the near-surface soils of oil and gas reservoirs, while the dominant microbial communities were Gram-positive bacteria (Mycobacterium and Rhodococcus) in background soil. Real-time quantitative polymerase chain reaction (PCR) results furthermore showed that the abundance of alkB genes increased substantially in the surface soils above oil and gas reservoirs even though only low or undetectable concentrations of hydrocarbons were measured in these soils. The results of this study implicate that trace amounts of volatile hydrocarbons migrate from oil and gas reservoirs, and likely result in the changes of microbial communities in the near-surface soil.


1984 ◽  
Vol 24 (1) ◽  
pp. 278
Author(s):  
H. T. Pecanek ◽  
I. M. Paton

The Tirrawarra Oil and Gas Field, discovered in 1970 in the South Australian portion of the Cooper Basin, is the largest onshore Permian oil field in Australia. Development began in 1981 as part of the $1400 million Cooper Basin Liquids ProjectThe field is contained within a broad anticline bisected by a north-south sealing normal fault. This fault divides the Tirrawarra oil reservoir into the Western and Main oil fields. Thirty-four wells have been drilled, intersecting ten Patchawarra Formation sandstone gas reservoirs and the Tirrawarra Sandstone oil reservoir. Development drilling discovered three further sandstone gas reservoirs in the Toolachee Formation.The development plan was based on a seven-spot pattern to allow for enhanced oil recovery by miscible gas drive. The target rates were 5400 barrels of oil (860 kilolitres) per day with 13 million ft3 (0.37 million m3) per day of associated gas and 70 million ft3 (2 million m') per day of wet, non-associated gas. Evaluation of early production tests showed rapid decline. The 100 ft (30 m) thick, low-permeability Tirrawarra oil reservoir was interpreted as an ideal reservoir for fracture treatment and as a result all oil wells have been successfully stimulated, with significant improvement in well production rates.The oil is highly volatile but miscibility with carbon dioxide has been proven possible by laboratory tests, even though the reservoir temperature is 285°F (140°C). Pilot gas injection will assess the feasibility of a larger-scale field-wide pressure maintenance scheme using miscible gas. Riot gas injection wells will use Tirrawarra Field Patchawarra Formation separator gas to defer higher infrastructure costs associated with the alternative option of piping carbon dioxide from Moomba, the nearest source.


Author(s):  
Yandong Zhou ◽  
Facheng Wang

Fixed platform have been widely employed in the offshore oil and gas reservoirs development. Pile foundation reliability is critical for these platforms where drilling, production and other functions are integrated. The lifting operation for the long pile, being a key step in the jacket installation, has been considered for further developments. With deep water developments, the sizes and weights of long piles are reasonably bigger. The corresponding process and equipment employed are subsequently altered, which brings challenges to developing a cost-effective, easy-operable approach for lifting operation. In this paper, the technology for the offshore long pile upending lifting operation including pile feature, installation methodology, lifting rigging and analysis model, covering water depths ranging from shallow to near deep water zone (60–300 m water depth) has been suggested. In addition, the applicability of the adoptable novel approaches has been discussed considering the practical project experience.


2021 ◽  
Author(s):  
Seyed Hossein Hashemi ◽  
Abas Niknam ◽  
Amir Karimian Torghabeh

Abstract Mineral ions are present in aqueous solutions in most industrial and operational processes, including oil operation. Accurate analysis and sampling of the formation water and its dissolved minerals during the operation of the oil industry can be a valuable solution for the efficient management of oil production from the reservoir. Therefore, in this study, evaluation of inorganic ions and their concentration in formation water for 6 well samples in the Rag-e Sefid Oil and Gas Field was considered. According to the results of this study, calcium, sodium and magnesium cations as well as sulfate, bicarbonate and chloride anions are soluble inorganic ions in the Rag Sefid Oil Field Formation. Also, in this study formation of inorganic sediment CaSO4, CaSO4.2H2O, CaCO3 and MgCO3 was studied. Based on the operating conditions of the Rag-e Sefid Oil and Gas field, the formation of calcium sulfate and calcium carbonate mineral deposits is significant. With base of geochemical analysis in reservoir rock samples and ions ratios these reservoir is good for preservation. The results thus provide more accurate predictions in terms of where to find gas reservoirs in the Zagros basin, and can lead to significantly better exploitation of these resources and also estimation of rate of sedimentation for EOR.


2021 ◽  
Author(s):  
Kambiz Razminia ◽  
Alain C. Gringarten

Abstract Objectives/Scope Single well deconvolution (von Schroeter et al., 2001) has been added to the well test interpretation toolbox nearly twenty years ago. In recent years, the single well deconvolution algorithm has been extended to multiple interfering wells (Cumming et al., 2013), and further improved with the additions of constraints to account for existing a-priory knowledge on the reservoir (constrained multiwell deconvolution, Cumming et al., 2019). The main objective of multiwell deconvolution is to identify the signatures of all wells involved and the interference signals between wells, from which information can be extracted about the reservoir that may not be obtainable otherwise, e.g. heterogeneities, boundaries and compartmentalization. The single well deconvolution algorithm has also been shown to be capable of restoring erroneous or missing rates (Gringarten, 2010). As shown in this paper, the same is true with multiwell deconvolution, which is able to restore erroneous or missing rates in all the wells involved. Methods, Procedures, Process Starting with arbitrary initial guesses for the missing rates in the various wells involved, we use multiwell deconvolution to estimate these missing flow rates or correct for erroneous ones. Two methods are presented: (1) we use unconstrained multiwell deconvolution as a first step to estimate the missing/erroneous rates, then use constrained multiwell deconvolution with these rates to estimate deconvolved derivatives; and (2) we restore/correct the flow rates and derive deconvolved derivatives simultaneously using constrained multiwell deconvolution. We show that the first approach is more accurate than the second one. In both approaches, we only obtain rates that are proportional to the true flow rates. To obtain the true flow rates, we need to know either one of the actual flow rates in each well, or the corresponding permeabilities. Results, Observations, Conclusions We prove the ability of multiwell deconvolution to estimate rates on synthetic oil reservoirs and gas reservoirs with moderate average reservoir pressure depletion, that include non-interfering wells. We then apply to oil and gas field examples and compare restored vs. actually measured rates. In all cases, the agreement is very good. Novel/Additive Information Using only measured pressure data, constrained multiwell deconvolution can be used to restore unknown flow rates and/or correct for erroneous rates, in addition to estimating deconvolved derivatives of all wells. This is particularly useful in the case of allocated rates or when rates are missing in some of the interfering wells.


2013 ◽  
Vol 734-737 ◽  
pp. 1381-1390
Author(s):  
Heng Song ◽  
Zi Fei Fan ◽  
Lun Zhao ◽  
An Gang Zhang

Zhanazhol oilfield is a large-scale complicated carbonated oil and gas field , Гnorth is the main oil and gas reservoirs of the oil field, The gas cap index is 0.38, the gas cap on a high condensate content. Reservoir development for nearly 25 years, exploitation in the past only to oil ring. Due to insufficient water injection in early age, the oil ring pressure dropped substantially, and the formation pressure to maintain the level of only 58%. For oil and gas reservoirs with a condensate gas cap, gas cap and oil ring at the same pressure system, with the decline in the pressure of the oil ring, the gas cap continue to spread to the oil region, while there are a large number of condensate oil anti-condensate from the gas cap, which loss into the formation. In this paper, the authors consider the characteristics of the oil and gas reservoirs and research the technique policy of collaborative development, These are all in order to solve technical problems, which is keep the pressure balance between the gas cap and oil ring during collaborative development. Not only provide technical to support the rational and efficient development of the Г North oil and gas reservoirs, but also provide a stable source for natural gas pipeline from Kazakhstan to China.


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