A novel bioflocculant for chromium (Ⅵ) and arsenic (Ⅲ) removal: Extracellular polymeric substance (EPS) of Pseudomonas fluorescens
<p>Bioflocculants produced by microorganisms have advantages in removing heavy metals because of their environmental friendliness and biodegradability. In this study, the bioflocculant from a soil bacteria was investigated for its application in chromium&#160;(&#8549;)&#160;and&#160;arsenic&#160;(&#8546;) removal. The bioflocculant-producing strain was indentified as Pseudomonas fluorescens. It showed maximum flocculating activity of 2579.94 U/mL and yield of 4.84 g/L under optimal condition. With a fed&#8209;batch fermentation strategy, bioflocculant production was further enhanced by 32.6%. The bioflocculant was as extracellular polymer substance composed of 76.67% polysaccharides and 15.8% protein with a molecular weight of 117 kDa. It showed excellent capacities in heavy metal removal, 80.13 and 45.93 mg/g for chromium(&#8549;) and arsenic (&#8546;), respectively. The bioflocculant outperformed conventional adsorption materials and could represent a promising biotechnology for the remediation of environmental problems.</p>