Land-use changes influence soil bacterial communities in a meadow grassland in Northeast China

Abstract. The conversion of natural grassland into agricultural fields is an intensive anthropogenic perturbation commonly occurring in semiarid regions, and this perturbation strongly affects soil microbiota. In this study, the influences of land-use conversion on the soil properties and bacterial communities in the Horqin Grasslands in Northeast China were assessed. This study aimed to investigate (1) how the abundances of soil bacteria changed across land-use types, (2) how the structure of the soil bacterial community was altered in each land-use type, and (3) how these variations were correlated with soil physical and chemical properties. Variations in the diversities and compositions of bacterial communities and the relative abundances of dominant taxa were detected in four distinct land-use systems, namely, natural meadow grassland, paddy field, upland field, and poplar plantation, through the high-throughput Illumina MiSeq sequencing technique. The results indicated that land-use changes primarily affected the soil physical and chemical properties and bacterial community structure. Soil properties, namely, organic matter, pH, total N, total P, available N and P, and microbial biomass C, N, and P, influenced the bacterial community structure. The dominant phyla and genera were almost the same among the land-use types, but their relative abundances were significantly different. The effects of land-use changes on the structure of soil bacterial communities were more quantitative than qualitative.

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Land-use changes influence soil bacterial communities in a meadow grassland in Northeast China

10.5194/se-2017-69 ◽

2017 ◽

Author(s):

Chengyou Cao ◽

Ying Zhang ◽

Wei Qian ◽

Caiping Liang ◽

Congmin Wang ◽

...

Keyword(s):

Land Use ◽

Bacterial Community ◽

Bacterial Communities ◽

Land Use Changes ◽

Chemical Properties ◽

Soil Bacterial Communities ◽

Land Use Types ◽

Soil Bacterial ◽

Physical And Chemical ◽

And Chemical Properties

Abstract. The conversion of natural grassland into agricultural fields is an intensive anthropogenic perturbation commonly occurring in semi-arid regions, and this perturbation strongly affects soil microbiota. In this study, the influences of land-use conversion on the soil properties and bacterial communities in Horqin Grasslands in Northeast China were assessed. This study aimed to investigate (1) how the abundances of soil bacteria changed across land-use types; (2) how the structure of soil bacterial community was altered in each land-use type; and (3) how these variations were correlated with soil physical and chemical properties. The variations in diversities and compositions of bacterial communities and relative abundance of dominant taxa were detected in four distinct land-use systems, namely, natural meadow grassland, paddy field, upland field, and poplar plantation, through high-throughput Illumina MiSeq sequencing technique. Results indicated that land-use changes primarily affected soil physical and chemical properties and bacterial community structure. Soil properties, namely, organic matter, pH, total N, total P, available N and P, and microbial biomass C, N, and P, influenced the bacterial community structure. The dominant phyla and genera were almost the same among the land-use types, but their relative abundances were significantly different. The effects of land-use changes on the structure of soil bacterial communities were more quantitative than qualitative.

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Environmental and Anthropogenic Factors Shape Major Bacterial Community Types Across the Complex Mountain Landscape of Switzerland

Frontiers in Microbiology ◽

10.3389/fmicb.2021.581430 ◽

2021 ◽

Vol 12 ◽

Author(s):

Johanna Mayerhofer ◽

Daniel Wächter ◽

Pierluigi Calanca ◽

Lukas Kohli ◽

Tobias Roth ◽

...

Keyword(s):

Land Use ◽

Environmental Factors ◽

Bacterial Community ◽

Bacterial Communities ◽

Bipartite Network ◽

Network Analyses ◽

Soil Bacterial Communities ◽

Land Use Types ◽

Biogeographic Regions ◽

Soil Bacterial

Mountain areas harbor large climatic and geographic gradients and form numerous habitats that promote high overall biodiversity. Compared to macroorganisms, knowledge about drivers of biodiversity and distribution of soil bacteria in mountain regions is still scarce but a prerequisite for conservation of bacterial functions in soils. An important question is, whether soil bacterial communities with similar structures share environmental preferences. Using metabarcoding of the 16S rRNA gene marker, we assessed soil bacterial communities at 255 sites of a regular grid covering the mountainous landscape of Switzerland, which is characterized by close location of biogeographic regions that harbor different land-use types. Distribution of bacterial communities was mainly shaped by environmental selection, as revealed by 47.9% variance explained by environmental factors, with pH (29%) being most important. Little additional variance was explained by biogeographic regions (2.8%) and land-use types (3.3%). Cluster analysis of bacterial community structures revealed six bacterial community types (BCTs), which were associated to several biogeographic regions and land-use types but overall differed mainly in their preference for soil pH. BCT I and II occurred at neutral pH, showed distinct preferences for biogeographic regions mainly differing in elevation and nutrient availability. BCT III and IV differed only in their preferred soil pH. BCT VI occurred in most acidic soils (pH 3.6) and almost exclusively at forest sites. BCT V occurred in soils with a mean pH of 4 and differed from BCT VI in preference for lower values of organic C, total nitrogen and their ratio. Indicator species and bipartite network analyses revealed 3,998 OTUs associating to different levels of environmental factors and BCTs. Taxonomic classification revealed opposing associations of taxa deriving from the same phyla. The results revealed that pH, land-use type, biogeographic region, and nutrient availability were the main factors shaping bacterial communities across Switzerland. Indicator species and bipartite network analyses revealed environmental preferences of bacterial taxa. Combining information of environmental factors and BCTs yielded increased resolution of the factors shaping soil bacterial communities and provided an improved biodiversity framework. OTUs exclusively associated to BCTs provide a novel resource to identify unassessed environmental drivers.

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Plant–Soil Feedback In Camellia Oleifera And Mixed Gardenia Jasminoides Ellis–Camellia Oleifera Stands Determined By Soil Bacterial Community Analysis

10.21203/rs.3.rs-849971/v1 ◽

2021 ◽

Author(s):

Yun Wang ◽

Peng Xie ◽

Jiyun She ◽

Aihua Deng ◽

Shaogang Fan

Keyword(s):

Bacterial Community ◽

Chemical Properties ◽

Physicochemical Characteristics ◽

Soil Bacterial Community ◽

Physical And Chemical Properties ◽

Camellia Oleifera ◽

Gardenia Jasminoides ◽

Soil Bacterial ◽

Physical And Chemical ◽

And Chemical Properties

Abstract Purpose: Little is known regarding the combined impact of plant and soil traits on the soil bacterial community. Herein, we assessed physical and chemical properties along with bacterial community structure in soils sampled at different depths (0–20 cm, 20–40 cm, and 40–60 cm) and slope positions (peak, hillside, and bottom), in Camellia oleifera monoculture and mixed Gardenia jasminoides–Camellia oleifera stands. Methods: Soil physicochemical characteristics were determined using standard methods. The composition of soil bacterial communities was evaluated using high-throughput sequencing of the 16S rRNA gene. Results: Soil organic carbon, humus, and total organic contents were higher in G. jasminoides + C. oleifera low-yielding forest than in other stands, however, the NH4+-N levels were significantly lower than that in monoculture. The slope position did not greatly influence soil physical and chemical properties. The dominant bacteria were Proteobacteria, Chloroflexi, Acidobacteria, and Actinobacteria. The alpha and beta diversity and abundance of soil bacterial community were higher in intercropping systems than in monoculture systems. Potassium and nitrogen levels and pH significantly affected the soil microbial community composition. Correlation analysis revealed that alkaline hydrolysable nitrogen and pH were significantly correlated with the input of plant-associated organic matter and dynamic changes of keystone taxa.Conclusion: G. jasminoides improved the physicochemical characteristics of C. oleifera low-yielding soil and greatly affected the soil bacterial community, further improving the soil microecological environment. Therefore, this specific intercropping system is an effective strategy for improving soil health.

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Variation of soil nutrients and bacterial community diversity of different land utilization types in Yangtze River Basin, Chongqing Municipality

PeerJ ◽

10.7717/peerj.9386 ◽

2020 ◽

Vol 8 ◽

pp. e9386

Author(s):

Yanlin Li ◽

Chunmei Zeng ◽

Meijun Long

Keyword(s):

Land Use ◽

River Basin ◽

Yangtze River ◽

Yangtze River Basin ◽

Chemical Properties ◽

Physical And Chemical Properties ◽

Chongqing Municipality ◽

Land Use Types ◽

Physical And Chemical ◽

And Chemical Properties

The diversity and community distribution of soil bacteria in different land use types in Yangtze River Basin, Chongqing Municipality were studied by using Illumina MiSeq analysis methods. Soil physical and chemical properties were determined, and correlation analyses were performed to identify the key factors affecting bacterial numbers and α-diversity in these soils. The results showed that the soil physical and chemical properties of different land use types decrease in the order: mixed forest (M2) > pure forest (P1) > grassland (G3) > bare land (B4). There were significant differences in bacterial diversity and communities of different land use types. The diversity of different land use types showed the same sequence with the soil physical and chemical properties. The abundance and diversity of bacterial in M2 and P1 soils was significantly higher than that in G3 and B4 soils. At phylum level, G3 and B4 soils were rich in only Proteobacteria and Actinobacteria, whereas M2 and P1 soils were rich in Proteobacteria, Actinobacteria and Firmicutes. At genus level, Faecalibacterium and Agathobacter were the most abundant populations in M2 soil and were not found in other soils. Pearson correlation analysis showed that soil moisture content, pH, AN, AP, AK and soil enzyme activity were significantly related to bacterial numbers, diversity and community distribution.

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The Influence of Land Use Patterns on Soil Bacterial Community Structure in the Karst Graben Basin of Yunnan Province, China

Forests ◽

10.3390/f11010051 ◽

2019 ◽

Vol 11 (1) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

Jiangmei Qiu ◽

Jianhua Cao ◽

Gaoyong Lan ◽

Yueming Liang ◽

Hua Wang ◽

...

Keyword(s):

Land Use ◽

Surface Layer ◽

Bacterial Community ◽

Bacterial Communities ◽

Yunnan Province ◽

Soil Bacterial Community ◽

Land Use Patterns ◽

Use Patterns ◽

Soil Bacterial Communities ◽

Soil Bacterial

Land use patterns can change the structure of soil bacterial communities. However, there are few studies on the effects of land use patterns coupled with soil depth on soil bacterial communities in the karst graben basin of Yunnan province, China. Consequently, to reveal the structure of the soil bacterial community at different soil depths across land use changes in the graben basins of the Yunnan plateau, the relationship between soil bacterial communities and soil physicochemical properties was investigated for a given area containing woodland, shrubland, and grassland in Yunnan province by using next-generation sequencing technologies coupled with soil physicochemical analysis. Our results indicated that the total phosphorus (TP), available potassium (AK), exchangeable magnesium (E-Mg), and electrical conductivity (EC) in the grassland were significantly higher than those in the woodland and shrubland, yet the total nitrogen (TN) and soil organic carbon (SOC) in the woodland were higher than those in the shrubland and grassland. Proteobacteria, Verrucomicrobia, and Acidobacteria were the dominant bacteria, and their relative abundances were different in the three land use types. SOC, TN, and AK were the most important factors affecting soil bacterial communities. Land use exerts strong effects on the soil bacterial community structure in the soil’s surface layer, and the effects of land use attenuation decrease with soil depth. The nutrient content of the soil surface layer was higher than that of the deep layer, which was more suitable for the survival and reproduction of bacteria in the surface layer.

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The effects of afforestation on soil bacterial communities in temperate grassland are modulated by soil chemical properties

PeerJ ◽

10.7717/peerj.6147 ◽

2019 ◽

Vol 7 ◽

pp. e6147 ◽

Cited By ~ 1

Author(s):

Shu-Hong Wu ◽

Bing-Hong Huang ◽

Jian Gao ◽

Siqi Wang ◽

Pei-Chun Liao

Keyword(s):

Bacterial Community ◽

Bacterial Communities ◽

Chemical Properties ◽

Soil Chemical Properties ◽

Soil Microbiome ◽

Apparent Lack ◽

Soil P ◽

Soil Bacterial Communities ◽

Abundant Otus ◽

Soil Bacterial

Grassland afforestation dramatically affects the abiotic, biotic, and ecological function properties of the original ecosystems. Interference from afforestation might disrupt the stasis of soil physicochemical properties and the dynamic balance of microbiota. Some studies have suggested low sensitivity of soil properties and bacterial community to afforestation, but the apparent lack of a significant relationship is probably due to the confounding effects of the generalist habitat and rare bacterial communities. In this study, soil chemical and prokaryotic properties in a 30-year-old Mongolia pine (Pinus sylvestris var. mongolica Litv.) afforested region and adjacent grassland in Inner Mongolia were classified and quantified. Our results indicate that the high richness of rare microbes accounts for the alpha-diversity of the soil microbiome. Few OTUs of generalist (core bacteria) and habitat-specialist bacteria are present. However, the high abundance of this small number of OTUs governs the beta-diversity of the grassland and afforested land bacterial communities. Afforestation has changed the soil chemical properties, thus indirectly affecting the soil bacterial composition rather than richness. The contents of soil P, Ca2+, and Fe3+ account for differentially abundant OTUs such as Planctomycetes and subsequent changes in the ecologically functional potential of soil bacterial communities due to grassland afforestation. We conclude that grassland afforestation has changed the chemical properties and composition of the soil and ecological functions of the soil bacterial community and that these effects of afforestation on the microbiome have been modulated by changes in soil chemical properties.

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Soil bacterial community and functional shifts in response to altered snowpack in moist acidic tundra of northern Alaska

SOIL ◽

10.5194/soil-2-459-2016 ◽

2016 ◽

Vol 2 (3) ◽

pp. 459-474 ◽

Cited By ~ 10

Author(s):

Michael P. Ricketts ◽

Rachel S. Poretsky ◽

Jeffrey M. Welker ◽

Miquel A. Gonzalez-Meler

Keyword(s):

Climate Change ◽

Bacterial Community ◽

Bacterial Communities ◽

Bacterial Community Structure ◽

Chemical Properties ◽

Winter Precipitation ◽

Snow Accumulation ◽

Soil Microbial ◽

Soil Bacterial Communities ◽

Soil Bacterial

Abstract. Soil microbial communities play a central role in the cycling of carbon (C) in Arctic tundra ecosystems, which contain a large portion of the global C pool. Climate change predictions for Arctic regions include increased temperature and precipitation (i.e. more snow), resulting in increased winter soil insulation, increased soil temperature and moisture, and shifting plant community composition. We utilized an 18-year snow fence study site designed to examine the effects of increased winter precipitation on Arctic tundra soil bacterial communities within the context of expected ecosystem response to climate change. Soil was collected from three pre-established treatment zones representing varying degrees of snow accumulation, where deep snow ∼ 100 % and intermediate snow ∼ 50 % increased snowpack relative to the control, and low snow ∼ 25 % decreased snowpack relative to the control. Soil physical properties (temperature, moisture, active layer thaw depth) were measured, and samples were analysed for C concentration, nitrogen (N) concentration, and pH. Soil microbial community DNA was extracted and the 16S rRNA gene was sequenced to reveal phylogenetic community differences between samples and determine how soil bacterial communities might respond (structurally and functionally) to changes in winter precipitation and soil chemistry. We analysed relative abundance changes of the six most abundant phyla (ranging from 82 to 96 % of total detected phyla per sample) and found four (Acidobacteria, Actinobacteria, Verrucomicrobia, and Chloroflexi) responded to deepened snow. All six phyla correlated with at least one of the soil chemical properties (% C, % N, C : N, pH); however, a single predictor was not identified, suggesting that each bacterial phylum responds differently to soil characteristics. Overall, bacterial community structure (beta diversity) was found to be associated with snow accumulation treatment and all soil chemical properties. Bacterial functional potential was inferred using ancestral state reconstruction to approximate functional gene abundance, revealing a decreased abundance of genes required for soil organic matter (SOM) decomposition in the organic layers of the deep snow accumulation zones. These results suggest that predicted climate change scenarios may result in altered soil bacterial community structure and function, and indicate a reduction in decomposition potential, alleviated temperature limitations on extracellular enzymatic efficiency, or both. The fate of stored C in Arctic soils ultimately depends on the balance between these mechanisms.

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Assessment of Basalt Soil Quality under Different Land Use Types in Bao Loc - Di Linh Area, Lam Dong Province

VNU Journal of Science Earth and Environmental Sciences ◽

10.25073/2588-1094/vnuees.4117 ◽

2017 ◽

Vol 33 (3) ◽

Author(s):

Thủy Nguyễn Thị ◽

Anh Thế Lưu

Keyword(s):

Land Use ◽

Chemical Properties ◽

Natural Forest ◽

Physical And Chemical Properties ◽

Forest Land ◽

Land Use Types ◽

Physical And Chemical ◽

Soil Unit ◽

And Chemical Properties

Basaltic soil is considered as the most advantageous soil unit in comparison with other units of the Central Highlands, that distributed mainly in the plateaus of Kon Plong, Kon Ha Nung, Pleiku, Buon Ma Thuot, M'Drak, Dak Nong and Di Linh - Bao Loc. Much of the basaltic soil in the Central Highlands has been used for cultivation of long-term industrial crops. Currently, due to massive forest destruction for developing long-term industrial trees in the basaltic soil in Bao Loc - Di Linh, the natural fertility of the basaltic soil has been remarkably reduced. The physical and chemical properties of the basaltic soil under different land use types have decreased sharply compared to basaltic soil under the natural forest. The average rate of decline of total organic matter content of plantation forest land is 16%, overused forest land is 44%, tea land is 46%, coffee land is 60% compared to the same soil unit under the natural forest. The cation exchange capacity (CEC), content of total nutrients and plant available nutrients under the land use types were also decreased significantly compared to the soil unit under the natural forest. The changes of physical and chemical properties of the basaltic soil with coffee cultivation was highest in comparison with other land use types. The organic matter and potassium are two limiting factors of the nutrients in the basaltic soil of the study area, especially for tea cultivation.

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