scholarly journals An Exploration of the Electrical Properties of Agarose Gel: Characterization of Concentration Using Nyquist Plot Phase Angle and the Implications of a More Comprehensive In Vitro Model of the Brain

2013 ◽  
Vol 20 (3) ◽  
Author(s):  
Roland Pomfret ◽  
Karl Sillay ◽  
Gurwattan Miranpuri
Keyword(s):  
Electrical Properties ◽  
Phase Angle ◽  
In Vitro Model ◽  
Nyquist Plot ◽  
Agarose Gel ◽  
Vitro Model ◽  
The Brain

In vitro model for predicting the access and distribution of drugs in the brain using hCMEC/D3 cells

2021 ◽  
Author(s):  
Bárbara Sánchez-Dengra ◽  
Isabel González-Álvarez ◽  
Flavia Sousa ◽  
Marival Bermejo ◽  
Marta González-Álvarez ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Vitro Model ◽  
The Brain

scholarly journals Delivery of Thyronamines (TAMs) to the Brain: A Preliminary Study

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1616
Author(s):  
Nicoletta di Leo ◽  
Stefania Moscato ◽  
Marco Borso' ◽  
Simona Sestito ◽  
Beatrice Polini ◽  
...  
Keyword(s):  
High Performance ◽  
In Vitro Model ◽  
New Drugs ◽  
Therapeutic Approaches ◽  
Neuroprotective Effects ◽  
Pharmacological Response ◽  
Preliminary Study ◽  
Vitro Model ◽  
The Brain

Recent reports highlighted the significant neuroprotective effects of thyronamines (TAMs), a class of endogenous thyroid hormone derivatives. In particular, 3-iodothyronamine (T1AM) has been shown to play a pleiotropic role in neurodegeneration by modulating energy metabolism and neurological functions in mice. However, the pharmacological response to T1AM might be influenced by tissue metabolism, which is known to convert T1AM into its catabolite 3-iodothyroacetic acid (TA1). Currently, several research groups are investigating the pharmacological effects of T1AM systemic administration in the search of novel therapeutic approaches for the treatment of interlinked pathologies, such as metabolic and neurodegenerative diseases (NDDs). A critical aspect in the development of new drugs for NDDs is to know their distribution in the brain, which is fundamentally related to their ability to cross the blood–brain barrier (BBB). To this end, in the present study we used the immortalized mouse brain endothelial cell line bEnd.3 to develop an in vitro model of BBB and evaluate T1AM and TA1 permeability. Both drugs, administered at 1 µM dose, were assayed by high-performance liquid chromatography coupled to mass spectrometry. Our results indicate that T1AM is able to efficiently cross the BBB, whereas TA1 is almost completely devoid of this property.

Characterization of the mechanisms of electrochemotherapy in an in vitro model for human cervical cancer

2005 ◽  
Author(s):  
Bujian Tang ◽  
Li Li ◽  
Zhifeng Jiang ◽  
Yingzi Luan ◽  
Danrong Li ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
In Vitro Model ◽  
Vitro Model ◽  
Human Cervical Cancer

scholarly journals Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death

2014 ◽  
pp. 95-103
Author(s):  
Mathieu Vinken ◽  
Michaël Maes ◽  
Sara Crespo Yanguas ◽  
Joost Willebrords ◽  
Tamara Vanhaecke ◽  
...  
Keyword(s):  
Cell Death ◽  
In Vitro Model ◽  
Vitro Model

Characterization of chaotropic effects of detergents on lipids homeostasis in an in vitro model of ocular toxicity: a molecular network based-lipidomic approach

2021 ◽  
Vol 350 ◽  
pp. S129-S130
Author(s):  
R Magny ◽  
K. Kessal ◽  
A. Regazzetti ◽  
O. Laprévote ◽  
C. Baudouin ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Molecular Network ◽  
Ocular Toxicity ◽  
Vitro Model

Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)

2016 ◽  
Vol 133 ◽  
pp. 100-112 ◽  
Author(s):  
Victor Llombart ◽  
Teresa García-Berrocoso ◽  
Joan Josep Bech-Serra ◽  
Alba Simats ◽  
Alejandro Bustamante ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Stable Isotope ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Isotope Labeling ◽  
Vitro Model

Development of an in vitro model of calcified cerebral emboli in acute ischemic stroke for mechanical thrombectomy evaluation

2020 ◽  
Vol 12 (10) ◽  
pp. 1002-1007
Author(s):  
Sarah Johnson ◽  
Ray McCarthy ◽  
Brian Fahy ◽  
Oana Madalina Mereuta ◽  
Seán Fitzgerald ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
In Vitro Model ◽  
Mechanical Thrombectomy ◽  
Ex Vivo ◽  
Guide Catheter ◽  
Vitro Model ◽  
Calcified Tissues

​BackgroundCalcified cerebral emboli (CCEs) are a rare cause of acute ischemic stroke (AIS) and are frequently associated with poor outcomes. The presence of dense calcified material enables reliable identification of CCEs using non-contrast CT. However, recanalization rates with the available mechanical thrombectomy (MT) devices remain low.ObjectiveTo recreate a large vessel occlusion involving a CCE using an in vitro silicone model of the intracranial vessels and to demonstrate the feasability of this model to test different endovascular strategies to recanalize an occlusion of the M1 segment of the middle cerebral artery (MCA).​MethodsAn in vitro model was developed to evaluate different endovascular treatment approaches using contemporary devices in the M1 segment of the MCA. The in vitro model consisted of a CCE analog placed in a silicone neurovascular model. Development of an appropriate CCE analog was based on characterization of human calcified tissues that represent likely sources of CCEs. Feasibility of the model was demonstrated in a small number of MT devices using four common procedural techniques.​ResultsCCE analogs were developed with similar mechanical behavior to that of ex vivo calcified material. The in vitro model was evaluated with various MT techniques and devices to show feasibility of the model. In this limited evaluation, the most successful retrieval approach was performed with a stent retriever combined with local aspiration through a distal access catheter, and importantly, with flow arrest and dual aspiration using a balloon guide catheter.​ConclusionCharacterization of calcified tissues, which are likely sources of CCEs, has shown that CCEs are considerably stiffer than thrombus. This highlights the need for a different in vitro AIS model for CCEs than those used for thromboemboli. Consequentially, an in vitro AIS model representative of a CCE occlusion in the M1 segment of the MCA has been developed.

Morphological characterization of the adherence and invasion ofStreptococcus agalactiaeto the intestinal mucosa of tilapiaOreochromissp.: An in vitro model

2019 ◽  
Vol 42 (9) ◽  
pp. 1223-1231 ◽  
Author(s):  
Gersson Vásquez‐Machado ◽  
Paola Barato‐Gómez ◽  
Carlos Iregui‐Castro
Keyword(s):  
Intestinal Mucosa ◽  
In Vitro Model ◽  
Morphological Characterization ◽  
Vitro Model
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