scholarly journals Anti-aging Activity of Cream Ethanol Extract of Ethyl Acetate Fraction of Purple Passion Fruit Peel (Passiflora edulis Sims.)

Author(s):  
Julia Reveny ◽  
. Sudarmi ◽  
Herawaty Ginting
2019 ◽  
Vol 2 (1) ◽  
pp. 07-12
Author(s):  
Sony Eka Nugraha ◽  
Suryadi Achmad ◽  
Erly Sitompul

North Sumatera is one of the central areas of purple passion fruit production in Indonesia. Processing passion fruit into beverage products (passion fruit juice) produces peel  has not been utilized. The use of passion fruit skin needs to be studied so that it can be useful as a raw material for antibacterial drug preparations. The aim of this study was to determine the phytochemical constituent screening  and antibacterial activity of ethyl acetate fraction of purple passion fruit peel against Staphylococcus aureus and Escherichia coli. Simplicia and ethyl acetate fraction were determinated  its phytochemical properties.  The extraction process by percolation method using ethanol  96% and continue to fractionation process by liquid liquid extraction method using n-hexane and ethyl acetate. The antibacterial activity were tested  against Staphylococcus aureus and Escherichia coli using agar diffusion method with paper discs. The result showed  that  the simplicia characteristic of passion fruit peel  were water content of  8.64%, water soluble extract of  31.69%, ethanol soluble extract of 13.02%, ash total of 7.89%, and insoluble ash in acid of 0.816%. The phytochemical screening simplicia and ethyl acetate fraction showed the presence of flavonoids, glycosides, saponins and tannins. The antibacterial activity test showed that the ethyl acetate fraction has an effective inhibition at the concentration of 100 mg/ml against Staphylococcus aureus and Escherichia coli, it showed dose dependent manner. The ethyl acetate fraction of passion fruit peel (Passiflora edulis Sims) has an antibacterial activity  on Staphylococcus aureus and Escherichia coli


2018 ◽  
Vol 1 (1) ◽  
pp. 257-263
Author(s):  
Herawaty Ginting ◽  
Aminah Dalimunthe ◽  
Edo Kusda Pratama

Latar belakang: Buah markisah ungu (Passiflora edulis Sims.) banyak dikonsumsi masyarakat sebagai minuman yang segar. Kulit buah markisah berkhasiat sebagai antihipertensi, antimikroba dan antiinflamasi Tujuan penelitian: untuk mengetahui dosis toksik (LD50), sehingga aman untuk digunakan sebagai obat. Metode penelitian: menggunakan mencit sebanyak 25 ekor yang dibagi menjadi 5 kelompok, yaitu kelompok kontrol (Na-CMC 0,5%), kelompok perlakuan diberi ekstrak etanol kulit buah markisa ungu (EEKBMU) dengan variasi dosis 500, 1000, 2000 dan 5000 mg/kg bb). Pada akhir pengujian dilakukan pengamatan terhadap gejala toksik, berat badan, kematian hewan, serta pemeriksaan makropatologi dan gambaran histopatologi hati mencit. Hasil penelitian: pemberian EEKBMU (Passiflora edulis Sims.) untuk semua kelompok tidak ada hewan yang mati. Kelompok kontrol, dosis 500 mg/kg bb, 1000 mg/kg bb tidak menunjukkan gejala toksik tetapi pada dosis 2000 mg/kg bb dan 5000 mg/kg bb menunjukkan gejala toksik. Hasil pemeriksaan histopatologi organ hati mencit menunjukkan bahwa pemberian EEKBMU dosis 2000 mg/kg bb terjadi nekrosis hemorrhagia pada vena sentral, sedang dosis 5000 mg/kg bb terjadi nekrosis dan hemorrhagia yang meluas. Kesimpulan: EEKBMU dosis 2000 mg/kg bb dan 5000 mg/kg bb sangat toksik dan LD50 tidak dapat ditentukan Background: Purple passion fruit (Passifloraedulis Sims.) is consumed a lot as fresh drinks. The rinds of purple passion fruit can be used as anti-hypertension, anti-microbes, and anti-inflammatory. Research objectives: To determine the toxic dose (LD50) so it could be safe to use as medicine. Research method: The animals used were 25 mice which were divided into 5 groups, the control group (Na-CMC 0.5%), the treatment group given the purple passion fruit rinds ethanol extract with dose of of 500, 1000, 2000, and 5000 mg/kb bb. the observations of the signs of toxicity, weights, deaths, and macrophatology and histophatologyexaminations of mice liver were conducted. Findings: The purple passion fruit rinds ethanol extract given to all groups didn’t induce mortality. The control group with the dose of 500 mg/kb bb, 1000 mg/kb bb showed no sign of toxicity obeserved. However, at the dose of 2000 mg/kb bb, and 5000 mg/kb bb were found sign of toxicity. The histophatologyexamination of mice livers indicated that the dose of2000 mg/kb bb caused hemorrhagia necrosis to central vena and the dose of5000 mg/kb bb caused expanded necrosis and hemorrhagia. Conclusion: Thepurple passion fruit rinds ethanol extractat the doses of2000 mg/kb bb and 5000 mg/kb bb were very toxic and the LD50 could not be determined.


Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 231 ◽  
Author(s):  
Nohra C. Rodríguez ◽  
Luz M. Melgarejo ◽  
Matthew W. Blair

Purple passion fruit (Passiflora edulis Sims f. edulis) is a tropical juice source. The goal of this project was to evaluate photosynthetic and physiological variability for the crop with the hypotheses that landraces contain the diversity to adapt to higher elevation nontraditional growing environments and this is dependent on specific parameters of ecological adaptation. A total of 50 genotypes of this crop were chosen from divergent sources for evaluations of their eco-physiological responses in two equatorial locations at different altitudes in the Andes Mountains, a center of diversity for the species. The germplasm included 34 landraces, 8 commercial cultivars, and 8 genebank accessions. The two locations were contrasting in climates, representing mid and high elevations in Colombia. Mid-elevation valleys are typical regions of production for passion fruit while high elevation sites are not traditional. The location effects and variables that differentiated genotypes were determined. Results showed statistically significant differences between locations and importance of physiological parameters related to photosynthesis and water use efficiency. Some landraces exhibited better water status and gas exchange than commercial types. Parameters like maximum photosynthesis, points of light saturation and compensation, darkness respiratory rate, and apparent quantum yield varied between genotype groups. The landraces, commercial types, and genebank entries also differed in content of carotenoids and chlorophylls a and b. Meanwhile, photosynthesis measurements showed that altitudinal difference had an effect on genotype-specific plant growth and adaptation. An important conclusion was that landraces contained the diversity to adapt to the new growing environment at higher altitudes.


Fruits ◽  
2014 ◽  
Vol 69 (1) ◽  
pp. 19-30 ◽  
Author(s):  
Alemwati Pongener ◽  
Vidyaram Sagar ◽  
Ram Krishna Pal ◽  
Ram Asrey ◽  
Ram Roshan Sharma ◽  
...  

2018 ◽  
Vol 1 (2) ◽  
pp. 29-34
Author(s):  
Sony Eka Nugraha ◽  
Suryadi Achmad ◽  
Erly Sitompul

Abstract.North Sumatera is the central production of  purple passion fruit  in Indonesia. Passion fruit was  processing into beverage product that produced passion fruit peel waste. The passion fruit peel has potential effect as a sources of medicine which is need to be examined such as its antibacterial effect. The purpose of this study was to determine the characterization of simplicia, phytochemical screening  and antibacterial activity of ethanol extract of purple passion fruit peel against Staphylococcus aureus and Escherichia coli. The simplicia powder was examined its characteristic include water content, water soluble extract, ethanol soluble extract, total ash and acid insoluble ash assay. The extraction was process by percolation method using ethanol  96% , then tested antibacterial activity against Staphylococcus aureus and Escherichia coli using agar diffusion method with paper discs.The result showed  that  the simplicia characteristic of passion fruit peel  were water content of  8.64%, water soluble extract of  31.69%, ethanol soluble extract of 13.02%, ash total of 7.89%, and insoluble ash in acid of 0.816%. The phytochemical screening simplicia and ethanol extract showed the presence of flavonoid, glycoside, saponin, tannin and steroid/triterpenoid.The antibacterial activity test showed that the ethanol extract has an effective inhibition at the  concentration of 300 mg/ml against Staphylococcus aureus and Escherichia coli, it showed dose dependent manner.   Keywords: Passiflora edulis Sims, antibacterial, passion fruit peel, Staphylococcus aureus and Escherichia coli        


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