The comparison of two Chemometric Assisted UV Spectrophotometric Techniques with High-performance Liquid Chromatography Methods for simultaneous determination of three Antiemetic drugs used in Chemotherapy Induced Nausea and Vomiting

2021 ◽  
pp. 4815-4824
Author(s):  
Veena Devi Singh ◽  
Vijay Kumar Singh ◽  
Sanjay J Daharwal
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Multivariate Calibration ◽  
Partial Least Square ◽  
Nausea And Vomiting ◽  
Control Analysis ◽  
Calibration Methods ◽  
Significant Difference

This research work was emphasis to adopt green analytical chemistry via development of environmentally friendly methods for simultaneously estimation of drugs used for chemotherapy induced nausea and vomiting (CINV). In these study two multivariate calibration methods namely; Partial least square (PLS) models, Principal component regression (PCR) and RP-HPLC (Reverse phase- high performance liquid chromatography) method were employed for simultaneous assessment of aprepitant (APT), dexamethsone (DEX) and ondansetron (OND) in their market formuations. The chromatographic separation was achieved on Phenomenex Luna C18 column (150mm X 4.6mm in diameter with 5μ particle size) and detection was carried out by UV-Visible detector. The mobile phase comprises a mixture of ethanol and toluene in a ratio of 65:35% v/v, at the flow rate of 1.2ml/ min. The elution was monitored at 225nm and total run time required for separation was 10 min. The retention time of APT, DEX and OND were found to be 4.37 min, 6.57 and 8.11 min respectively. The Applied methods were validated as per ICH guidelines to achieve maximum sensitivity and lowest error. Linearity range for multivariate calibration methods was found to be 5-50μg/mL for APT, DEX and OND and similarly for HPLC methods was found to be 5-30μg/mL,5-30μg/mL and 5-50μg/mL of APT, DEX and OND respectively. A statistical procedure was carried out to find statistical difference among these developed methods. The results revealed that there is no significant difference between two multivariate models and HPLC methods. Therefore, it could be applied as an alternative of HPLC method in quality control laboratories lacking the required facilities for these expensive instruments. Hence, proposed method can also be applied in clinical pharmacy, toxicology and quality control analysis without the interference of commonly encountered dosage form additives.

Simultaneous Determination of Six Components in Jingzhiguanxin Tablet by High-Performance Liquid Chromatography

2019 ◽  
Vol 15 (2) ◽  
pp. 130-137
Author(s):  
Hui Jiang ◽  
Lianhao Fu ◽  
Yu Wang ◽  
Shaozhi Wang ◽  
Xiaoxu Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gradient Elution ◽  
Tanshinone Iia ◽  
Array Detector ◽  
Control Analysis ◽  
Active Components ◽  
Quality Control Analysis

Background: Jingzhiguanxin (JZGX) tablet, a traditional Chinese prescription, is commonly used for treating coronary heart disease and angina pectoris in the clinic. There are six active components (Danshensu (DSS), Protocatechuic aldehyde (PD), Paeoniflorin (PF), Ferulic acid (FA), Salvianolic acid B (Sal B) and Tanshinone IIA (TA)) in JZGX tablet. </P><P> Objective: In this paper, a simple and reliable method was used for simultaneous determining the six active components by high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Methods: These six active components were separated on an Agilent Zorbax Eclipse XDB-C18 column (150 mmx4.6 mm, 5 µm) at 30 °C. Acetonitrile (A), methanol (B) and 0.5% H3PO4 aqueous solution (C) were used as mobile phase for gradient elution. The flow rate was 1 mL/min and the detection wavelengths were set at 280 nm for DSS, PD and Sal B, 230 nm for PF, 320 nm for FA and 270 nm for TA, respectively. Results: All of the six components showed good linearity regressions (r2≥0.9997) in the detected concentration range. The recovery rates and coefficient of variation (CV) for all analytes were 98.66%- 100.18% and 0.75%-1.89%, respectively. This method was successfully applied to simultaneously determine the six components in JZGX tablet from different batches and manufacturers. Conclusion: The validated method can be used in routine quality control analysis of JZGX tablet without any interference.

Development of Simultaneous Analysis Method for Multi-Compounds Content of New Shilajit Using HPLC-UV and the Cognitive Enhancing Effect: Mongolian Shilajit

2021 ◽  
Vol 16 (7) ◽  
pp. 1934578X2110304
Author(s):  
SukJin Lee ◽  
HyeSung Ryu ◽  
WanKyunn Whang
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Radical Scavenging ◽  
Original Material ◽  
Analysis Method ◽  
Quality Control Standard ◽  
Quantitative Hplc Analysis

Shilajit has a longstanding use as an anti-aging and memory enhancing drug. It is known to have excellent anti-bacterial effects and is believed to be effective for cognitive enhancement, but is difficult to standardize because of the lack of quality control standards. This study, for the first time, proposes a quality control standard using a simultaneous analytical method for the drug’s multi-compound content using high-performance liquid chromatography-ultraviolet detection (HPLC-UV) as an aid for the internationalization of Mongolian Shilajit. Phenolic compounds 1-6 were isolated from Mongolian Shilajit extract using bioassay-guided isolation, and the isolated compounds were evaluated for cognitive-related anti-Alzheimer’s disease (AD) activities using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), β-site amyloid precursor protein-cleaving enzyme 1 (BACE1), and advanced glycation end-product (AGE) formation assays. The isolated compounds showed good effects for each activity. In addition, the isolated compounds were successfully quantified using a validated quantitative HPLC analysis method. As a result, the isolated compounds were suggested as standard marker compounds for Mongolian Shilajit. Also, we proved that the original material of Mongolian Shilajit is a lichen named Xanthoparmelia somloensis (Gyel.) Hale using HPLC-UV, ultra-high-performance liquid chromatography-electrospray ionization/hybrid linear trap-quadruple-orbitrap-high-resolution mass spectrometry (UHPLC-ESI/LTQ-HRMS).

scholarly journals Quality control of traditional chinese drugs by high performance liquid chromatography.

1986 ◽  
Vol 35 (3) ◽  
pp. 202-206 ◽  
Author(s):  
Nobuyuki HITOMI ◽  
Kesamitsu SHIMIZU ◽  
Keiichi YAMAMOTO ◽  
Osamu MIURA ◽  
Eizaburo YUMIOKA
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Chinese Drugs ◽  
Traditional Chinese Drugs

scholarly journals Influence of spray mixture volume and flight height on herbicide deposition in aerial applications on pastures

2014 ◽  
Vol 32 (1) ◽  
pp. 227-232 ◽  
Author(s):  
M.A.P. Oliveira ◽  
U.R. Antuniassi ◽  
E.D. Velini ◽  
R.B. Oliveira ◽  
J.F. Salvador ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Mineral Oil ◽  
Mato Grosso ◽  
Significant Difference ◽  
Flight Height ◽  
Mixture Volume ◽  
Pasture Area ◽  
Experimental Plots

The objective of the present study was to analyze the influence of spray mixture volume and flight height on herbicide deposition in aerial applications on pastures. The experimental plots were arranged in a pasture area in the district of Porto Esperidião (Mato Grosso, Brazil). In all of the treatments, the applications contained the herbicides aminopyralid and fluroxypyr (Dominum) at the dose of 2.5 L c.p. ha-1, including the adjuvant mineral oil (Joint Oil) at the dose of 1.0 L and a tracer to determine the deposition by high-performance liquid chromatography (HPLC) (rhodamine at a concentration of 0.6%). The experiment consisted of nine treatments that comprised the combinations of three spray volumes (20, 30 and 50 L ha-1) and three flight heights (10, 30 and 40 m). The results showed that, on average, there was a tendency for larger deposits for the smallest flight heights, with a significant difference between the heights of 10 and 40 m. There was no significant difference among the deposits obtained with the different spray mixture volumes.

Fast high-performance liquid chromatography method for quality control of soy extracts

2004 ◽  
Vol 1038 (1-2) ◽  
pp. 107-112 ◽  
Author(s):  
Sandra Apers ◽  
Tania Naessens ◽  
Katleen Van Den Steen ◽  
Filip Cuyckens ◽  
Magda Claeys ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Chromatography Method ◽  
Liquid Chromatography Method

scholarly journals ROBUSTNESS EVALUATION OF HPLC DETERMINATION OF ATORVASTATIN AND LISINOPRIL ON COLUMN PUROSPHER C8 STAR IN PHARMACEUTICALS

2021 ◽  
pp. 21-26
Author(s):  
N. S. Shulyak ◽  
A. D. Abbeyquaye ◽  
D. B. Koval
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Analytical Methods ◽  
High Performance ◽  
Antihypertensive Drugs ◽  
Hplc Method ◽  
Fixed Dose ◽  
Control Analysis ◽  
Chromatography Method ◽  
Pharmaceutical Development

Introduction. Innovative pharmaceutical development of various antihypertensive drugs with statins and the creation of domestic fixed-dose combinations of drugs with different effects is an urgent task of modern pharmacy, which will help attract more patients to the treatment and prevention of cardiovascular disease. Pharmaceutical development of atorvastatin and lisinopril by our scientific group proposes for using the ratio of (1/1) for lisinopril (10 mg) and atorvastatin (10 mg). HPLC (High-Performance Liquid Chromatography) technique is adopted as it is considered as the most common technique in realm of quality control analysis. The aim of the study – to evaluate the robustness of HPLC (High-Performance Liquid Chromatography) method for the quantitation of lisinopril and atorvastatin and determine the analytical parameters that present greater influence in the final results of the analysis. Research Methods. An efficient method to assess the robustness of analytical methods is by Youden’s test, by means of an experiment design which involves seven analytical parameters combined in eight tests. In the recent studies, we assessed the robustness of a chromatographic method to quantify lisinopril and atorvastatin in tablets using Youden’s test. Results and Discussion. By using the criteria of Youden’s test, HPLC method proved to be greatly robust regarding content of lisinopril and atorvastatin, when variations in seven analytical parameters were introduced. The most variation in effects of the analytical parameters in retention time (Rt) for lisinopril and atorvastatin HPLC quantitation was when used column supplier. Purospher C8 STAR (55 mm x 4mm, 5 μm) is based on high purity silica and an almost complete surface coverage. Purospher C8 STAR provides excellent peak symmetry for acidic, basic and even chelating compounds, highest column efficiency in terms of the number of theoretical plates, and exceptional stability from pH 1.5 to 10.5. Conclusion. Youden’s test can be applied successfully for the ro­bustness evaluation in validation process of analytical methods and results ontained in our work should be interest to the scientific population dealing with pharmaceutical analytical chemistry.

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