Effect of Kinetin on Growth and Development of Microtubers of Potato (Solanum Tuberosum L.)

2021 ◽  
Vol 1 (4) ◽  
pp. 48-54
Author(s):  
Kumari Meenakshi ◽  

The present study was conducted at Central Research Potato Institute, Campus, Modipuram, India to assess the effect of kinetin on the growth and development of microtubers formation in vitro in two potato varieties Kufri Bahar and Kufri Surya. The lower concentration of kinetin (0.75 mg/l) showed decreased parameters like the number of microtubers, fresh weight of microtubers, eyes per microtuber. Higher concentration of kinetin (1.5 mg/l and 2.25 mg/l) led to increasing parameters as compared to control but decreased shoot length in both the varieties but kinetin at (2.25 mg/l) concentration gave best results for in vitro microtuberization for quality and yield parameters.

2019 ◽  
Vol 6 (03) ◽  
Author(s):  
TS MISHRA ◽  
NK MISHRA ◽  
VK MISHRA ◽  
US MISHRA ◽  
HM SINGH

A field experiment was conducted at Allahabad in order to evaluate the performance of different Potato (Solanum tuberosum L.) varieties under the climatic conditions of Northern Plains of India.The experiment was carried out for two consecutive years i.e., during 2015-16 and 2016-17to assess the morphology, physiological behaviour of these potato varieties/strains under a similar set of agro-climatic conditions. All the potato varieties/strainsviz.E-4486,JI-5857, JI-1804, JI-1808, JG-224, JG-657,JE-808, JF-110, JF-27, JF-547,JG-1134, Fr/B-10, JH-222, EM/H- 1601,JF-4864, JF-5106, JF-4915,JH-49,JH-516,JI-5871,FR-B-4,FR-B-4,FR/B-21,FR.B-105,G-2524,Kufri Sheetman, Kufri Chandramukhi, Kufri Bahar, Kufri Lalima, Kufri Badshah, Kufri Jyoti, Kufri Alankar and Kufri Sinduri were replicated thrice under randomized block design. Important information on different aspects like tuber sprouting, plant growth and development and yield was gathered in all the treatments.Potato variety JH-222 was the best performer followed by JG-224, E-4486, JF-547, Kufri Bahar, FR/B-10, Kufri Badshah, JF-110, Kufri Chandramukhi, EM/H-1601, Kufri Alankar, JF-27, JG-1334 and JE-808.


Author(s):  
Ksenia Guseva ◽  
Irina Borodulina ◽  
Elena Myakisheva ◽  
Otari Tavartkiladze

2015 ◽  
Vol 33 (3) ◽  
pp. 315-321
Author(s):  
Md. Nazmul Haque ◽  
Md. Hazrat Ali ◽  
Tuhin Suvra Roy ◽  
Sheikh Muhammad Masum ◽  
Imtiaz Faruk Chowdhury

The different levels of arsenic (As) had a significant effect on the yield, yield reduction and As accumulation of different potato varieties. The yield was negatively affected by the As contamination and decreased with the increasing As levels in the soil, but remained statistically similar up to 25 mg kg-1 soil of As and thereafter drastically decreased with the increasing As levels. The yield reduction (%) and accumulation of As in the tuber peels and flesh increased with the increasing As levels. Among the fourteen potato varieties, 'Felsina' had the maximum yield and showed the lowest percentage of yield reduction; 'Jam alu' and 'Cardinal' accumulated the least amount of As in their peels and flesh, respectively. Among the treatment combinations, 'Felsina' cultivated in an As-free soil had the highest yield/plant (454.8 g fresh weight). 'Laura' grown in 25 mg kg-1 soil of As showed the lowest yield reduction (%). Although 'Jam alu' and 'Cardinal' produced a slightly lower yield compared to some other varieties, these two varieties accumulated the least amount of As, both in the peels and flesh, when grown in 25 mg kg-1 soil of As.


Author(s):  
Tanuja Buckseth ◽  
R.K. Singh ◽  
Ashwani K. Sharma ◽  
Sumita Sharma ◽  
Vaishali Moudgil ◽  
...  

2009 ◽  
Vol 55 (No. 6) ◽  
pp. 252-256 ◽  
Author(s):  
R. Asghari-Zakaria ◽  
B. Maleki-Zanjani ◽  
E. Sedghi

In order to investigate the effects of soluble chitosan on plantlets growth <I>in vitro</I> and increase of minituber yield in potato micropropagation, plantlets of Agria cultivar were treated <I>in vitro</I>with soluble chitosan at different concentrations including 0, 5, 15, 50, 150, 500, 750 and 1000 mg/l added to the MS tissue culture medium. Plantlets were subsequently transferred to the greenhouse and minituber yield parameters were evaluated. At the concentrations of 750 and 1000 mg/l of chitosan the culture medium failed to solidify. Application of 500 mg/l of soluble chitosan increased the shoot fresh weight, but its lower concentrations did not significantly affect this trait (<I>P</I> < 0.05). The 5 and 15 mg/l of soluble chitosan led to a significant increase in root fresh and dry weight of <I>in vitro</I> plantlets, whereas, higher concentrations, especially 500 mg/l, significantly decreased root fresh weight of <I>in vitro</I> plantlets. Application of 500 mg/l chitosan <I>in vitro</I> resulted in improved acclimatization of plantlets in the greenhouse as expressed by significant (<I>P</I> < 0.05) increase in minituber number and yield, compared to the control. The tested lower concentrations had no effect on yield parameters. The present results indicate that soluble chitosan can be successfully incorporated into potato seed production from <I>in vitro</I> plantlets.


2020 ◽  
Vol 7 (1) ◽  
pp. 8-25
Author(s):  
Kadi Zahia ◽  
Rached Kanouni Malika ◽  
Khammar Sara ◽  
Chebout Abderrezeq ◽  
Khammar Hichem ◽  
...  

AbstractThis work investigates the interactive effect of six culture media and three photoperiods (darkness, 16h/8h, 8h/16h) on the microtuberization of four potato varieties (Spunta, Désirée, Kondor and Bartina). The objective is to determine the best tuberization under these growing conditions. The measured parameters which are related to the suitability of the tuberization characteristics are: the morphological aspects (shape, position) and the biometric ones (number and diameter of tubers). The obtained results permit to valorize the meristems that constitute the starting explant, and their good organogenetic skills to provide the first generation of micropropagation with healthy vitroplants in a sufficient quantity. Thus, the grown vitroplants on the medium (MS/2+BAP+COU) presented the best values which are related to the percentage of tuberization, the number of microtubercles / vitroplants and weight of tubers. Moreover, the Bartina genotype showed a remarkable superiority over its media and under a 16h/8h photoperiod except for the diameter of the tubers, where this genotype had the best diameter over the medium (MS/2+KIN) under an 8h photoperiod.


2017 ◽  
Author(s):  
Yunita Prameswari ◽  
FNU Djenal ◽  
FNU Damanhuri

Kebutuhan kentang yang semakin tinggi menyebabkan permintaan semakin meningkat. Rendahnya produksi kentang mengakibatkan berbagai upaya untuk peningkatan produksi terus dilakukan. Penggunaan metode kultur jaringan yaitu metode untuk mengisolasi bagian tanaman seperti protoplasma, sel, sekelompok sel, jaringan dan organ dalam kondisi aseptik, sehingga dapat diperbanyak dan beregenerasi menjadi tanaman utuh dapat dijadikan alternatif pemenuhan kebutuhan. Penelitian ini bertujuan untuk mengetahui kecepatan pembentukan umbi mikro kentang (Solanum Tuberosum L.) varietas granola kembang secarain vitro dengan menggunakan dua faktor dan 3 kali ulangan. Faktor pertama yaitu aspirin dengan tiga taraf (5,10,15) ppm. Faktor kedua yaitu kinetin dalam tiga taraf (8,10,12) ppm. Penelitian menggunakan seluruh propagul kentang yang berumur 30 hari setelah subkultur dan data yang didapat dianalisis menggunakan ANOVA. Hasil penelitian menunjukan bahwa interaksi aspirin dan kinetin tidak berpengaruh terhadap jumlah akar, kedinian umbi, dan bobot umbi. Interaksi perlakuan terbaik bagi pembentukan tunas yaitu A2K1 aspirin 10 ppm dan kinetin 8 ppm sedangkan Interaksi perlakuan terbaik pada parameter jumlah umbi yaituA3K2 aspirin 15 ppm dan kinetin 10.


2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.


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