Investigating Associations Between Proliferation Indices, C-kit, and Lymph Node Stage in Canine Mast Cell Tumors

2017 ◽  
Vol 53 (5) ◽  
pp. 258-264 ◽  
Author(s):  
Erika Lauren Krick ◽  
Matti Kiupel ◽  
Amy C. Durham ◽  
Tuddow Thaiwong ◽  
Dorothy C. Brown ◽  
...  
Keyword(s):  
Mast Cell ◽  
Lymph Node ◽  
Odds Ratio ◽  
Tandem Duplication ◽  
Stage Ii ◽  
Ki 67 ◽  
Internal Tandem Duplication ◽  
Mast Cell Tumors ◽  
Proliferation Indices ◽  
Grade Ii

ABSTRACT Previous studies have evaluated cellular proliferation indices, KIT expression, and c-kit mutations to predict the clinical behavior of canine mast cell tumors (MCTs). The study purpose was to retrospectively compare mitotic index, argyrophilic nucleolar organizer regions (AgNORs)/nucleus, Ki-67 index, KIT labeling pattern, and internal tandem duplication mutations in c-KIT between stage I and stage II grade II MCTs. Medical records and tumor biopsy samples from dogs with Grade II MCTs with cytological or histopathological regional lymph node evaluation were included. Signalment, tumor location and stage, and presence of a recurrent versus de novo tumor were recorded. Mitotic index, AgNORs/nucleus, Ki-67, KIT staining pattern, and internal tandem duplication mutations in exon 11 of c-KIT were evaluated. Sixty-six tumors (51 stage I; 15 stage II) were included. Only AgNORs/nucleus and recurrent tumors were significantly associated with stage (odds ratio 2.8, 95% confidence interval [CI] 1.0–8.0, P = .049; odds ratio 8.8, 95% CI 1.1–69.5; P = .039). Receiver-operator characteristic analysis showed that the sensitivity and specificity of AgNORs/cell ≥ 1.87 were 93.3% and 27.4%, respectively, (area under the curve: 0.65) for predicting stage. Recurrent tumors and higher AgNORs/nucleus are associated with stage II grade II MCTs; however, an AgNOR cutoff value that reliably predicts lymph node metastasis was not determined.

Radiation Therapy for Incompletely Excised Grade II Canine Mast Cell Tumors

2006 ◽  
Vol 42 (6) ◽  
pp. 430-434 ◽  
Author(s):  
Valerie Jasmine Poirier ◽  
William M. Adams ◽  
Lisa J. Forrest ◽  
Eric M. Green ◽  
Richard R. Dubielzig ◽  
...  
Keyword(s):  
Mast Cell ◽  
Lymph Node ◽  
Regional Lymph Node ◽  
Overall Survival Rate ◽  
Mast Cell Tumors ◽  
Mast Cell Tumor ◽  
Grade Ii ◽  
Cutaneous Mast Cell ◽  
Lymph Node Irradiation ◽  
Prophylactic Irradiation

Forty-five dogs with incompletely excised grade II mast cell tumors were treated with radiation using a cobalt 60 teletherapy unit (15 fractions of 3.2 Gy for a total of 48 Gy). Twenty-four of the dogs underwent prophylactic regional lymph node irradiation. Three (6.7%) dogs had tumor recurrence, two (4.4%) dogs developed metastasis, and 14 (31%) dogs developed a second cutaneous mast cell tumor. No difference in overall survival rate was observed between the dogs receiving and not receiving prophylactic irradiation of the regional lymph node.

scholarly journals Ancillary techniques on the evaluation of canine cutaneous mast cell tumors from Brazil

2016 ◽  
Vol 46 (10) ◽  
pp. 1804-1810 ◽  
Author(s):  
Mariana Martins Flores ◽  
Renata Dalcol Mazaro ◽  
Ingeborg Maria Langohr ◽  
Alma Roy ◽  
Keith Strother ◽  
...  
Keyword(s):  
Mast Cell ◽  
Pcr Amplification ◽  
The United States ◽  
Growth Fraction ◽  
Grading System ◽  
Low Grade ◽  
Ki 67 ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Exon 11

ABSTRACT: The use of histologic classification by a 2-tier grading system only, immunohistochemistry (IHC) for KIT and Ki-67 and polymerase chain reaction (PCR) for internal tandem duplications (ITD) on exon 11 has improved the prognostication of canine cutaneous mast cell tumors (CCMTs) particularly in the United States. However, these techniques are not commonly used in most Brazilian laboratories. Likewise, no studies, to date, have investigated the occurrence of ITD in CCMTs from the country. Thus, this study tested the 2-tier grading system, the immunohistochemistry for KIT and Ki-67 and the PCR for exon 11 in a group of Brazilian CCMTs with the goal of investigating the applicability of these tests in a Brazilian laboratory. Of the 39 CCMTs, 69.2% (27/39) were identified as low-grade and 30.8% (12/39) as high-grade by a 2-tier grading system. All tumors had a KIT expression pattern II, and 30.6% (11/36) had a high growth fraction (Ki-67). PCR amplification was successful in four of the 11 tumors examined. Two of these (50%) were positive for ITD. This study highlights the importance of using auxiliary techniques in the CCMT evaluation, identifies limitations and confirms the applicability of these methods on a routine diagnostic basis in Brazil. Our results will help to improve the prognostication of CCMTs in Brazilian diagnostic laboratories, encouraging the use of supplementary methods.

scholarly journals Effects of electrochemotherapy with cisplatin and peritumoral IL-12 gene electrotransfer on canine mast cell tumors: a histopathologic and immunohistochemical study

2017 ◽  
Vol 51 (3) ◽  
pp. 286-294 ◽  
Author(s):  
Claudia Salvadori ◽  
Tanja Svara ◽  
Guido Rocchigiani ◽  
Francesca Millanta ◽  
Darja Pavlin ◽  
...  
Keyword(s):  
Mast Cell ◽  
T Lymphocytes ◽  
Mhc Class Ii ◽  
Cellular Response ◽  
Histopathological Examination ◽  
Combined Treatment ◽  
Gene Electrotransfer ◽  
Neoplastic Cells ◽  
Ki 67 ◽  
Mast Cell Tumors

AbstractBackgroundThe study was aimed to characterize tumor response after combined treatment employing electrochemotherapy with IL-12 gene electrotransfer in dogs with spontaneous mast cell tumors (MCT).Materials and methodsEleven dogs with eleven MCTswere included in the study. Histological changes were investigated in biopsy specimens collected before the treatment (T0), and 4 (T1) and 8 weeks (T2) later. Cellular infiltrates were characterized immunohistochemically by using anti CD3, CD20, Foxp3 (Treg), CD68 and anti MHC-class II antibodies. Proliferation and anti-apoptotic activity of neoplastic cells were assessed using anti Ki-67 and Bcl-2 antibodies. Angiogenetic processes were investigated immunohistochemically by using anti Factor VIII and anti CD31 antibodies and micro vessel density quantification.ResultsHistopathological examination of samples at T0confirmed the diagnosis and the presence of scanty infiltrates consisted mainly of T-lymphocytes and macrophages. At T1and T2neoplastic cells were drastically reduced in 7/11 cases, small clusters of neoplastic cells were detected in 3/11 cases and 1/11 cases neoplastic cells were still evident. Proliferation activity of neoplastic cells was significantly reduced at T1and T2and expression of anti-apoptotic protein at T1. Microvessel density was drastically reduced in all samples after treatment. The number of T-lymphocytes increased at T1, although not significant, while Treg were significant higher at T1and macrophages at T2.ConclusionsThe combined electrochemotherapy and IL-12 gene electrotransfer effectively induced a cellular response against neoplastic cells characterized mainly by the recruitment of T-lymphocytes and macrophages and a fibrotic proliferation with reduction of microvessels.

scholarly journals Prognosis of Grade II Cutaneous Mast Cell Tumors in 17 Dogs

2003 ◽  
Vol 56 (2) ◽  
pp. 87-91
Author(s):  
Teruo ITOH ◽  
Kiyotaka KUSHIMA ◽  
Kazumi NIBE ◽  
Kazuyuki UCHIDA ◽  
Hiroki SHII
Keyword(s):  
Mast Cell ◽  
Mast Cell Tumors ◽  
Grade Ii ◽  
Cutaneous Mast Cell

Recurrence Rates and Sites for Grade II Canine Cutaneous Mast Cell Tumors Following Complete Surgical Excision

2002 ◽  
Vol 38 (1) ◽  
pp. 71-73 ◽  
Author(s):  
Chick Weisse ◽  
Frances S. Shofer ◽  
Karin Sorenmo
Keyword(s):  
Mast Cell ◽  
Tumor Recurrence ◽  
Surgical Excision ◽  
Adjuvant Radiation ◽  
Recurrence Rates ◽  
Mast Cell Tumors ◽  
Complete Surgical Excision ◽  
Grade Ii ◽  
Cutaneous Mast Cell ◽  
Distant Tumor

A retrospective study was performed on 31 dogs with completely excised, grade II, cutaneous mast cell tumors in order to determine recurrence rates and sites. Distant tumor recurrence developed in 22% of dogs, and local tumor recurrence developed in 11% of dogs; however, the vast majority of these animals were incompletely staged initially. Complete surgical excision of grade II mast cell tumors was associated with effective local control in 89% of these dogs. Therefore, adjuvant radiation therapy might not be indicated in the majority of dogs with complete surgical excision.

Chirurgie subkutaner Mastzelltumoren beim Hund: Prognosefaktoren und Outcome

2021 ◽  
Vol 49 (03) ◽  
pp. 228-228
Author(s):  
Daniela Simon Betz
Keyword(s):  
Mast Cell ◽  
Clinical Outcome ◽  
Proliferating Cell Nuclear Antigen ◽  
Prognostische Faktoren ◽  
Microvascular Density ◽  
Nuclear Antigen ◽  
Prognostic Indicators ◽  
Ki 67 ◽  
Mast Cell Tumors ◽  
Proliferating Cell

Gill V, Leibman N, Monette S et al. Prognostic Indicators and Clinical Outcome in Dogs with Subcutaneous Mast Cell Tumors. J Am Anim Hosp Assoc 2020; 56 (4): 215–225 Mastzelltumoren (MZT) sind häufige kutane Neoplasien beim Hund, für die verschiedene prognostische Faktoren beschrieben wurden. Zu diesen zählen der histologische Grad nach Patnaik (3 Grade) oder Kiupel (2 Grade) sowie einige Marker zellulärer Differenzierung wie AgNORs (agyrophylic nuclear organizer regions), Ki-67 oder PCNA (proliferating cell nuclear antigen). Weitere prognostisch aussagekräftige Parameter sind der c-Kit-Mutationsstatus, die mikrovaskuläre Dichte (microvascular density, MVD) und der mitotische Index (MI). Ziel der Studie war, den klinischen Verlauf nach operativer Entfernung subkutaner MZT beim Hund auszuwerten und die Wertigkeit der verschiedenen Prognosefaktoren zu validieren.

Sign in / Sign up

Export Citation Format

Share Document