The molecular mechanism of mitotic arrest induced by a novel diterpenoid pseudolaric acid B and a novel gene encoding RNA-binding protein 22

2006 ◽  
Author(s):  
Kam-wai Wong
Keyword(s):  
Molecular Mechanism ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mitotic Arrest ◽  
Gene Encoding ◽  
Pseudolaric Acid B ◽  
Novel Gene

scholarly journals AUH, a gene encoding an AU-specific RNA binding protein with intrinsic enoyl-CoA hydratase activity.

1995 ◽  
Vol 92 (6) ◽  
pp. 2051-2055 ◽  
Author(s):  
J. Nakagawa ◽  
H. Waldner ◽  
S. Meyer-Monard ◽  
J. Hofsteenge ◽  
P. Jeno ◽  
...  
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Gene Encoding ◽  
Hydratase Activity ◽  
Enoyl Coa Hydratase

Molecular cloning and functional analysis of a salt-induced gene encoding an RNA-binding protein in alfalfa

2014 ◽  
Vol 34 (3) ◽  
pp. 1465-1473 ◽  
Author(s):  
Ruicai Long ◽  
Huiming Wang ◽  
Yixin Shen ◽  
Junmei Kang ◽  
Tiejun Zhang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Cloning ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Gene Encoding

Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines

2002 ◽  
Vol 293 (1) ◽  
pp. 150-154 ◽  
Author(s):  
Hong-Jin Shu ◽  
Takafumi Saito ◽  
Hisayoshi Watanabe ◽  
Jun-Itu Ito ◽  
Hiroaki Takeda ◽  
...  
Keyword(s):  
Cell Lines ◽  
Rna Binding ◽  
Binding Protein ◽  
Hepatoma Cell ◽  
Rna Binding Protein ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Gene Encoding ◽  
Hepatoma Cell Lines

scholarly journals The Fission Yeast RNA-Binding Protein Meu5 Is Involved in Outer Forespore Membrane Breakdown during Spore Formation

2020 ◽  
Vol 6 (4) ◽  
pp. 284
Author(s):  
Bowen Zhang ◽  
Erika Teraguchi ◽  
Kazuki Imada ◽  
Yuhei O. Tahara ◽  
Shuko Nakamura ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Target Genes ◽  
Rna Binding ◽  
De Novo ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Biological Significance ◽  
Spore Wall ◽  
Spore Formation ◽  
Membrane Breakdown

In Schizosaccharomyces pombe, the spore wall confers strong resistance against external stress. During meiosis II, the double-layered intracellular forespore membrane (FSM) forms de novo and encapsulates the nucleus. Eventually, the inner FSM layer becomes the plasma membrane of the spore, while the outer layer breaks down. However, the molecular mechanism and biological significance of this membrane breakdown remain unknown. Here, by genetic investigation of an S. pombe mutant (E22) with normal prespore formation but abnormal spores, we showed that Meu5, an RNA-binding protein known to bind to and stabilize more than 80 transcripts, is involved in this process. We confirmed that the E22 mutant does not produce Meu5 protein, while overexpression of meu5+ in E22 restores the sporulation defect. Furthermore, electron microscopy revealed that the outer membrane of the FSM persisted in meu5∆ spores. Investigation of the target genes of meu5+ showed that a mutant of cyc1+ encoding cytochrome c also showed a severe defect in outer FSM breakdown. Lastly, we determined that outer FSM breakdown occurs coincident with or after formation of the outermost Isp3 layer of the spore wall. Collectively, our data provide novel insights into the molecular mechanism of spore formation.

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