FORMULATION AND IN VITRO EVALUATION OF GEL BASED POLYHERBAL VAGINAL WASH

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (08) ◽  
pp. 25-30
Author(s):  
P. V. Powar ◽  
◽  
K. Kanade
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Stability Studies ◽  
Vaginal Infection ◽  
Short Term ◽  
Strong Activity ◽  
Term Stability ◽  
Vaginal Wash ◽  
In Vitro Antifungal Activity

Vaginal Infection is quite common in India, a multi-city study estimated that more than 90% of women between the ages 25 and 35 suffer with vaginal infection and 93% do not follow the best personal hygiene practices. The vaginal yeast infection is caused by Candida albicans. Which is characterized by Itching, irritation in tissues at the vaginal opening (vulva), burning sensation, redness /swelling of the vulva, vaginal pain ,soreness, vaginal rash, watery vaginal discharge . The objective of the present study was to develop poly-herbal vaginal wash for prevention of Candida albicans infection. Aqueous extracts of Azadirachtaindica leaf, Ocimum Sanctum leaf and Sapindus emarginatus were formulated in an aqueous based carbopol-940 (1%w/w) gel system. Prepared poly herbal vaginal wash formulation was evaluated for their physicochemical properties like texture evaluation, pH determination, viscosity and in vitro Antifungal activity were determined along with short term stability studies. The formulated gel based poly-herbal vaginal wash had acceptable physical parameters that showed that they were compatible and in addition to this, these formulations passed the short-term stability studies. The in vitro antifungal activity studies showed that the formulated gel based vaginal wash showed significantly strong activity against C. albicans. Thus, the present study concludes that the formulated herbal vaginal wash is efficient antifungal formulations for the C. albicans vaginal infection.

In vitro antifungal activity of eugenol and vanillin against Candida albicans and Cryptococcus neoformans

1982 ◽  
Vol 28 (11) ◽  
pp. 1235-1241 ◽  
Author(s):  
Chuenchit Boonchird ◽  
T. W. Flegel
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Tube Formation ◽  
Toxicity Tests ◽  
Short Term ◽  
Minimal Inhibitory Concentrations ◽  
The Mean ◽  
In Vitro Antifungal Activity

Eugenol and vanillin were examined for in vitro antifungal activity against the medically important yeasts, Candida albicans and Cryptococcus neoformans. Minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC) were determined for each compound against 31 strains of C. albicans and 33 strains of C. neoformans. With eugenol, the mean MIC's for C. albicans and C. neoformans were 625 and 293 μg/mL, respectively, while the mean MFC's were 1209 and 521 μg/mL, respectively. With vanillin, the mean MIC's for C. albicans and C. neoformans were 1250 and 738 μg/mL, respectively, while the mean MFC's were 5000 and 1761 μg/mL, respectively. With C. albicans, inhibition and retardation of growth were similar for yeast and mycelial forms, but germ tube formation was inhibited at concentrations lower than those which inhibited growth. Short-term toxicity tests with mice using the intraperitoneal route gave maximum tolerated doses of 62.5 mg/kg for eugenol and 125 mg/kg for vanillin and excluded their use as therapeutic agents for systemic mycoses.

scholarly journals Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 998
Author(s):  
Virgínia Barreto Lordello ◽  
Andréia Bagliotti Meneguin ◽  
Sarah Raquel de Annunzio ◽  
Maria Pía Taranto ◽  
Marlus Chorilli ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Enterococcus Faecium ◽  
Potato Starch ◽  
Oral Candidiasis ◽  
Candida Spp ◽  
Sem Images ◽  
Control Film ◽  
In Vitro Antifungal Activity

Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.

scholarly journals In Vitro Antifungal Activity of (1)-N-2-Methoxybenzyl-1,10-phenanthrolinium Bromide against Candida albicans and Its Effects on Membrane Integrity

Mycobiology ◽  
2017 ◽  
Vol 45 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Setiawati Setiawati ◽  
Titik Nuryastuti ◽  
Ngatidjan Ngatidjan ◽  
Mustofa Mustofa ◽  
Jumina Jumina ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Membrane Integrity ◽  
In Vitro Antifungal Activity

scholarly journals Antifungal Activity of Plumericin and Isoplumericin

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Dharmendra Singh ◽  
Umakant Sharma ◽  
Parveen Kumar ◽  
Yogesh K Gupta ◽  
M. P. Dobhal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Inhibitory Concentration ◽  
Chloroform Extract ◽  
Drug Candidate ◽  
Minimum Fungicidal Concentration ◽  
In Vitro Antifungal Activity

This study evaluated the in vitro antifungal activity of the chloroform extract of Plumeria bicolor and its phytoconstituents plumericin and isoplumericin against Candida species and Cryptococcus neoformans by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). Plumericin's consistently high activity against Candida albicans, C. krusei, C. glabrata, C. tropicalis and Cryptococcus neoformans was more potent than isoplumericin and the standard antifungal drug nystatin suggesting its potential as a drug candidate for candidiasis and cryptococcosis.

In vitro Antifungal Activity of Dihydropyrimidinones/Thiones Against Candida albicans and Cryptococcus neoformans

2020 ◽  
Vol 15 (6) ◽  
pp. 648-655
Author(s):  
Gabriel O. de Azambuja ◽  
Laura Svetaz ◽  
Itamar L. Gonçalves ◽  
Patricia F. Corbelini ◽  
Gilsane L. von Poser ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Drug Design ◽  
Cryptococcus Neoformans ◽  
Biginelli Reaction ◽  
Fungal Growth ◽  
Chemical Library ◽  
Antifungal Effect ◽  
In Vitro Antifungal Activity

Background: Since the Monastrol discovery in 1999 as the first inhibitor of Eg5, functionalized dihydropyrimidinones/thiones (DHPMs) have emerged as prototypes for drug design in different targets. The present work aimed to evaluate the antifungal activity of a chemical library of DHPMs. Methods: The compounds were obtained employing Biginelli reaction. Their antifungal activities were assessed against C. neoformans and C. albicans. Results: The compounds 1-i and 1-k inhibited moderately the fungal growth of C. neoformans, with compound 2-k presenting MIC80 values of 62.5-125 µg·mL-1. Considering activity against C. albicans, the compounds 1-i and 1-n present an MIC50 value of 125-250 µg·mL-1. Conclusion: The changes performed in DHPM scaffold appear to be valuable for generating compounds with potential antifungal effect.

scholarly journals In-Vitro Antifungal Activity of Nano Encapsulated Caprylic Acid and EFG1 Gene Expression Profile in Candida albicans

2021 ◽  
Vol 7 (3) ◽  
pp. 229-236
Author(s):  
Reyhaneh Zarimeidani ◽  
Shahla Roudbar mohammadi‎ ◽  
Maryam Roudbary ◽  
Fatemeh Nikoomanesh ◽  
Peyman Aslani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Caprylic Acid ◽  
In Vitro Antifungal Activity

scholarly journals In vitro antifungal activity of Turbinaria conoides collected from Mandapam coast, Tamilnadu, India

2016 ◽  
pp. 27-30
Author(s):  
A Shibu ◽  
S. Dhanam
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aspergillus Flavus ◽  
Inhibitory Activity ◽  
Marine Macroalgae ◽  
Traditional Medicines ◽  
Turbinaria Conoides ◽  
Fusarium Sp ◽  
In Vitro Antifungal Activity

ABSTRACT                 Marine macroalgae have been used as medicines or drug sources for a great many years, stretching back to the era of folk medicines. Algae have been extensively used in the traditional medicines of maritime nations for treatment of goitre, cancer, hypertension, cough and other diseases. The present work was carried out to find out the antifungal activity of the seaweed Turbinaria conoides collected from Mandapam coastal regions of Tamilnadu. The extracts were tested against Candida albicans, Candida parapsilopsis, Fusarium sp, Aspergillus flavus , Asergillus fumigatus. The hexane, chloroform and ethanolic extracts showed a well profound inhibitory activity against Candida albicans and Candida parapsilopsis. No inhibitory activity was found at Fusarium sp, Aspergillus flavus , Asergillus fumigates under chloroform and ethanolic extracts.Key words: Medicine, antifungal, cancer, Candida albicans, diseases.

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