Estimates of Genetic and Phenotypic Trends of Growth Traits in Bali Cattle

This research aims to develop a method for selection based on myostatin gene markers. The experiment was conducted in Bali cattle. Blood samples were collected from 100 cattle irrespective of age. PCR-RFLP was applied with primers for amplifying the 1346bp of the Myostatin gene were (F) 5’-CCCTACAGAGGCCACTTCAA-3’ and (R) 5’-CTCGCTGTTCTCATTCAGATC-3’, sand HaeIII restriction enzymes. PCR-RFLP results showed that not all genotypes present in the population, despite populations that were observed quite polymorphic (PIC = 49.5%) with the conditions of gene frequencies. The results showed that almost all variables influenced by myostatin genotype where the genotype BB is better, except the chest girth showed a better result in genotype AB. It suggested that MSTN is strong candidate gene that influenced growth traits. Moreover, it is also expected that this MSTN could be used in marker-assisted selection.

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Identification of single nucleotide polymorphism c.957A>C of PLAG1 gene and its association with growth traits in Bali cattle

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.46.3.199-208 ◽

2021 ◽

Vol 46 (3) ◽

pp. 199-208

Author(s):

I. G. R. Putra ◽

D. A. Sari ◽

S. M. Rachmawati ◽

R. Oktaviani ◽

R. R. Noor ◽

...

Keyword(s):

Growth Traits ◽

Direct Sequencing ◽

Average Daily Gain ◽

Single Nucleotide ◽

Exon 2 ◽

Sequencing Method ◽

Pcr Rflp ◽

Exon 1 ◽

Bali Cattle ◽

Daily Gain

The PLAG1 gene is one of the genes that affect the growth traits located on chromosome 14 in cattle. This study aims to obtain SNP of the PLAG1 gene in exon 1 and exon 2 and their association with growth traits in Bali cattle. The number of samples used was 52 samples of Bali cattle, 10 samples of Peranakan Ongole (PO), and 8 samples of Limousine cattle. Identification of SNPs PLAG1 gene was analyzed by direct sequencing method and genotyping of selected SNPs was carried out using PCR-RFLP. Association of genotypes of SNP c.957A>C with growth using t-test. There were 7 SNPs in exon 2 of the PLAG1 gene, namely SNP c.339A>G, c.489C>T, c.795A>G, c.957A>C, c.1023C>T, c.1056A>G, and c.1353A>G. SNP c.957A>C was validated by PCR-RFLP using TaqI enzyme and obtained three genotypes, namely genotypes AA, AC, and CC with allele frequency A and C, respec-tively 0.10 and 0.90 in Bali cattle, while in PO and Limousine cattle were monomorphic. Genotype association of SNP c.957A>C PLAG1 gene were not associated with birth weight (BW0), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365), yearling weight at 730 days of age (YW730), and average daily gain (ADG). SNP c.957A>C as a specific SNP for Bali cattle needs to be investigated in further research as a candidate marker for growth traits in Bali cattle.

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Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

Tropical Animal Science Journal ◽

10.5398/tasj.2019.42.3.175 ◽

2019 ◽

Vol 42 (3) ◽

pp. 175-179

Author(s):

D. E. W. T. Sihite ◽

R. Priyanto ◽

Jakaria Jakaria

Keyword(s):

Growth Traits ◽

Pcr Rflp ◽

Bali Cattle

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Phenotypic and Genetic Correlations of Growth Traits in Bali Cattle Breeding Population

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/372/1/012032 ◽

2019 ◽

Vol 372 ◽

pp. 012032

Author(s):

R Setiaji ◽

S Prastowo ◽

D Prasetiyo ◽

N Widyas

Keyword(s):

Growth Traits ◽

Genetic Correlations ◽

Breeding Population ◽

Cattle Breeding ◽

Bali Cattle

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Effect of Urea and Nano-Nitrogen Fertigation and Foliar Application of Nano-Boron and Molybdenum on some Growth and Yield Parameters of Potato

Al-Qadisiyah Journal For Agriculture Sciences (QJAS) (P-ISSN 2077-5822 E-ISSN 2617-1479) ◽

10.33794/qjas.vol10.iss1.107 ◽

2020 ◽

Vol 10 (1) ◽

pp. 253-263

Author(s):

Hayyawi W.A. Al-juthery ◽

Estabraq Hilal Obaid Al-Maamouri

Keyword(s):

Foliar Application ◽

Growth Traits ◽

Solanum Tuberosum L ◽

Block Design ◽

Growth And Yield ◽

Control Treatment ◽

Acid Yield ◽

Biological Yield ◽

Starch Yield ◽

Private Farm

Investigating the effect of urea and nano-nitrogen fertigation and foliar application of nano B and Mo on growth and yield of potato Solanum tuberosum L. [Rivera-A]. The study was conducted in a private farm located in the Al-Taleah area - Babylon governorate. The experiment consisted of (12) treatments consisting of separated fertigation of nano nitrogen (25% N) and urea (46% N), single treatments of leaf spraying of nano Mo (5%), Nano B (9%), nano-binary combinations (Mo+B) and (U+ Nano Mo), (U+Nano B), Nano (N+Mo), Nano (N+B), and tricombination treatments of (U+Mo+B), Nano (N+Mo+B) additional to the control treatment. Randomize Complete Block Design (RCBD) and one way simple experiment with three replicates. Fertilizers were applied at levels of 40 liters h-1 of Nano-N fertilizer (25% N) and 300 kg h-1 urea fertilizer (46% N). They were sprayed early in the morning after (40) days after planting four times. Two weeks is the period between an application and another according to the recommendation of (1) kg h-1 nano-fertilizer of (B) and (500) g h-1 of Mo. Fertilizers were injected and sprayed at (10, 20, 30 and 40)% of the total amount of the fertilizer were applied as the first, second, third and fourth applications, respectively. Some growth traits were tested including the chlorophyll content in the leaves, the total dry vegetative yild, the soft tubers yield, and the biological yield, proteins and ascorbic acid yield compared to the control (spray water only). The results of the Duncan test showed a significant increase in most of the studied traits of nano-tricombination (N+Mo+B) in the fresh tubers yield, dry vegetative yield , the biological yield, starch yield ,the total protein and ascorbic yield (37.53, 1.799, 8.138,4.152 , 481.3and 653.8 meg ha-1) respectively .compare to control (21.58 , 0.890, 4.463 ,2.323 , 366.1 and 215.5 meg ha-1) respectively.

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