scholarly journals Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

2019 ◽  
Vol 42 (3) ◽  
pp. 175-179
Author(s):  
D. E. W. T. Sihite ◽  
R. Priyanto ◽  
Jakaria Jakaria
Keyword(s):  
Growth Traits ◽  
Pcr Rflp ◽  
Bali Cattle

Polymorphisms of the Myostatin gene (MSTN) and its associationwith growth traits in Bali cattle

2017 ◽  
Author(s):  
Hary Nugroho ◽  
Woro Busono ◽  
Sucik Maylinda
Keyword(s):  
Growth Traits ◽  
Restriction Enzymes ◽  
Gene Frequencies ◽  
Gene Markers ◽  
Myostatin Gene ◽  
Pcr Rflp ◽  
Chest Girth ◽  
Bali Cattle ◽  
Strong Candidate ◽  
Almost All

This research aims to develop a method for selection based on myostatin gene markers. The experiment was conducted in Bali cattle. Blood samples were collected from 100 cattle irrespective of age. PCR-RFLP was applied with primers for amplifying the 1346bp of the Myostatin gene were (F) 5’-CCCTACAGAGGCCACTTCAA-3’ and (R) 5’-CTCGCTGTTCTCATTCAGATC-3’, sand HaeIII restriction enzymes. PCR-RFLP results showed that not all genotypes present in the population, despite populations that were observed quite polymorphic (PIC = 49.5%) with the conditions of gene frequencies. The results showed that almost all variables influenced by myostatin genotype where the genotype BB is better, except the chest girth showed a better result in genotype AB. It suggested that MSTN is strong candidate gene that influenced growth traits. Moreover, it is also expected that this MSTN could be used in marker-assisted selection.

scholarly journals Identification of single nucleotide polymorphism c.957A>C of PLAG1 gene and its association with growth traits in Bali cattle

2021 ◽  
Vol 46 (3) ◽  
pp. 199-208
Author(s):  
I. G. R. Putra ◽  
D. A. Sari ◽  
S. M. Rachmawati ◽  
R. Oktaviani ◽  
R. R. Noor ◽  
...  
Keyword(s):  
Growth Traits ◽  
Direct Sequencing ◽  
Average Daily Gain ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Sequencing Method ◽  
Pcr Rflp ◽  
Exon 1 ◽  
Bali Cattle ◽  
Daily Gain

The PLAG1 gene is one of the genes that affect the growth traits located on chromosome 14 in cattle. This study aims to obtain SNP of the PLAG1 gene in exon 1 and exon 2 and their association with growth traits in Bali cattle. The number of samples used was 52 samples of Bali cattle, 10 samples of Peranakan Ongole (PO), and 8 samples of Limousine cattle. Identification of SNPs PLAG1 gene was analyzed by direct sequencing method and genotyping of selected SNPs was carried out using PCR-RFLP. Association of genotypes of SNP c.957A>C with growth using t-test. There were 7 SNPs in exon 2 of the PLAG1 gene, namely SNP c.339A>G, c.489C>T, c.795A>G, c.957A>C, c.1023C>T, c.1056A>G, and c.1353A>G. SNP c.957A>C was validated by PCR-RFLP using TaqI enzyme and obtained three genotypes, namely genotypes AA, AC, and CC with allele frequency A and C, respec-tively 0.10 and 0.90 in Bali cattle, while in PO and Limousine cattle were monomorphic. Genotype association of SNP c.957A>C PLAG1 gene were not associated with birth weight (BW0), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365), yearling weight at 730 days of age (YW730), and average daily gain (ADG). SNP c.957A>C as a specific SNP for Bali cattle needs to be investigated in further research as a candidate marker for growth traits in Bali cattle.

scholarly journals Estimates of Genetic and Phenotypic Trends of Growth Traits in Bali Cattle

2012 ◽  
Vol 35 (2) ◽  
pp. 85-90
Author(s):  
A Gunawan ◽  
R Sari ◽  
Jakaria Jakaria
Keyword(s):  
Growth Traits ◽  
Bali Cattle

scholarly journals Association of Calpastatin (CAST) Gene with Growth Traits and Carcass Characteristics in Bali Cattle

2015 ◽  
Vol 38 (3) ◽  
pp. 145-149
Author(s):  
R. Putri ◽  
R. Priyanto ◽  
A. Gunawan ◽  
Jakaria Jakaria
Keyword(s):  
Growth Traits ◽  
Carcass Characteristics ◽  
Bali Cattle ◽  
Cast Gene

scholarly journals Melanocortin-4 Receptor (MC4R) gene polymorphism and its effect on growth traits in Madura cattle

2019 ◽  
Vol 44 (1) ◽  
pp. 38
Author(s):  
P. W. Prihandini ◽  
S. Sumadi ◽  
G. Suparta ◽  
D. Maharani
Keyword(s):  
Growth Traits ◽  
Balance Control ◽  
Direct Sequencing ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor ◽  
Pcr Rflp ◽  
Reverse Primer ◽  
Selection For

Melanocortin-4 receptor (MC4R) gene has an important role in the regulation of feed intake and energy balance control. The objective of this study was to identify the single nucleotide polymorphisms (SNPs) of MC4R gene and their association with growth traits in Madura cattle. A total of 198 calves were used in this study.Forward primer: 5’-GTCGGGCGTCTTGTTCATC-3’and reverse primer: 5’-GCTTGTGTTTAGCATCGCGT-3’ were used to amplify approximately 493 bp of MC4R gene. The results showed that two SNPs, g.1133C>G and g.1108C>T were identified by direct sequencing. The PCR-RFLP method was performed to genotype all individuals studied based on SNP g.1133C>G, and its SNP was significantly associated with shoulder height (SH) at yearling age (P<0.05). Animals with GG genotype had a higher SH (110.35±6.40cm) than those with CC (102.00±8.00 cm) and CG genotype (105.96±6.23 cm). The SNP g.1133 C>G changed amino acid from valine to leucine. In conclusion, the SNP g.1133C>G of the MC4R gene may be used as a marker-assisted selection for SH trait in Madura cattle.

scholarly journals Polymorphism of ADIPOQ and EDG1 genes in Indonesian beef cattle

2018 ◽  
Vol 43 (4) ◽  
pp. 323
Author(s):  
S. Sutikno ◽  
R. Priyanto ◽  
C. Sumantri ◽  
J. Jakaria
Keyword(s):  
Beef Cattle ◽  
Promoter Region ◽  
Marbling Score ◽  
Blood Samples ◽  
Adipoq Gene ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Cattle Blood ◽  
Bali Cattle ◽  
Novel Snp

The ADIPOQ and EDG1 genes were responsible in intramuscular fat deposition and marbling scores. This study was aimed to identify polymorphism of indel g.81966364D>I in promoter region of ADIPOQ gene and SNP c.-312A>G in 5' UTR of EDG1 gene in Indonesian beef cattle. Blood samples were collected from 211 cattle, including Bali (44), Madura (20), Pesisir (18), Katingan (20), PO (22), Pasundan (20), SO (12), Brahman (20), Simmental (15) and Limousin (18). Polymorphism of ADIPOQ gene was analyzed using PCR and direct sequencingmethods, whereas EDG1 gene was analyzed using PCR-RFLP (MscI enzyme) and direct sequencingmethods. Results of genotyping indel g.81966364D>I was monomorphic (DD genotype). The SNP c.-312A>G was polymorphic (AA and AG genotype) in Madura, Pesisir, Pasundan, Brahman, and Limousine. The Frequencies of allele A and G were 0.95, 0.92, 0.98, 0.95, 0.94 and 0.05, 0.08, 0.02, 0.05, 0.06 respectively. The values of Ho and He were 0.05-0.17 and 0.05-0.15 respectively and in Hardy-Weinberg equilibrium (P>0.05). In Bali, Katingan, PO, SO and Simmental were monomorphic (GG genotype). In Bali cattle, two novel SNP candidates were found in position of c.-399C>T and c.-273C>G which were potential to be used as genetic markers of marbling score for Bali cattle. As result this study, it can be concluded that ADIPOQ gene was similar while EDG1 gene was different in Indonesian beef cattle. in addition, found two candidates potential SNP in Bali cattle.

scholarly journals Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

2019 ◽  
Vol 26 (1) ◽  
pp. 44
Author(s):  
Endang Tri Margawati ◽  
Slamet Diah Volkandari ◽  
Indriawati Indriawati ◽  
Emma M. Svensson
Keyword(s):  
Genetic Variation ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Meat Tenderness ◽  
Gene Markers ◽  
Bos Javanicus ◽  
Restriction Fragment Polymorphism ◽  
Pcr Rflp ◽  
Bali Cattle ◽  
Cast Gene

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

scholarly journals Polymorphism Exploration of Growth Family (GH, GHRH and PIT-1) Genes Polymorphisms of Local Swamp Buffalo for Productivity Improvement in North Tapanuli Regency, North Sumatra

2021 ◽  
Vol 33 ◽  
pp. 02005
Author(s):  
Anneke Anggraeni ◽  
Chalid Thalib ◽  
Angga Ardhati Rani Hapsari
Keyword(s):  
Growth Traits ◽  
Restriction Enzymes ◽  
Economic Traits ◽  
Productivity Improvement ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Molecular Selection ◽  
Swamp Buffalo ◽  
Livestock Productivity ◽  
North Sumatra

Genetic improvement of livestock productivity can be done through molecular selection on the genes controlling growth traits. Genetic polymorphism of the growth family (GH, GHRH, and PIT1) genes were studied in local swamp buffalo (106 hds.) from a government buffalo breeding station (46 heads) and smallholders (60 heads) in North Tapanuli District, North Sumatra Province. Genotype variants of the three genes were identified by PCR-RFLP method using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene) and HinfI (PIT -1 gene). Genotyping on individual GH_g.1547T>C, GHRH_g.4666G>C, and PIT -1_g.1256G >A loci resulted only one type genotype, respectively TT, CC, an d AA, with one type of allele, respectively T, C, and A. Heterozygosity observation (Ho) and expectation (He) values values and the PIC value for each locus was 0.00. It could be suggested to increase genotype frequenciest of the three growth genes that are positively associated with the growth traits and economic traits of the buffalo.

scholarly journals Genetic Polymorphisms of Cyp19 and Myostatin Genes in Turkish Indigenous Sheep Breeds

2018 ◽  
Vol 68 (3) ◽  
pp. 313
Author(s):  
I. AKIŞ ◽  
F. ESEN GÜRSEL ◽  
N. HACIHASANOĞLU ÇAKMAK ◽  
G. ATMACA ◽  
H. YARDIBI ◽  
...  
Keyword(s):  
Growth Traits ◽  
Muscle Growth ◽  
Reproductive Activity ◽  
Meat Production ◽  
Production Traits ◽  
Sheep Breeds ◽  
Indigenous Sheep ◽  
Estrogen Synthesis ◽  
Pcr Rflp ◽  
Cyp19 Gene

Growth and meat production traits are very important in sheep breeding. Cyp19 gene has a major role in reproductive activity and growth due to its function in estrogen synthesis. Another gene affecting growth traits is  Myostatin (MSTN) gene, which mainly regulates skeletal muscle growth. In this study allele frequencies of genetic polymorphism in Cyp19 and Myostatin genes were identified by PCR-RFLP method in five indigenous Turkish sheep breeds, Chiose, Imroz, Kivircik, Zom and Morkaraman. Digestion of Cyp19 gene with HaeIII only revealed uncut AA genotype and digestion of MSTN with DraI also revealed only uncut AA genotype. Both loci analyzed in this study were found to be monomorphic in five Turkish indigenous sheep breeds. These highly conserved parts of the two genes can be useful for molecular evolutionary studies in sheep. Further studies regarding association analysis of Cyp19 and MSTN in sheep should be conducted.

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