scholarly journals Attachment and Proliferation of Human-Adipose-Tissue-Derived Stem Cells on Bioactive Glass/PVA Hybrid Scaffolds

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Viviane Gomide ◽  
Alessandra Arcoverde Cavalcanti Zonari ◽  
Natalia Martins Breyner ◽  
Alfredo Miranda de Goes ◽  
Marivalda M. Pereira
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Bioactive Glass ◽  
Pore Structure ◽  
Bone Repair ◽  
Sol Gel ◽  
Human Adipose Tissue ◽  
Porous Scaffolds

Bioactive glass/polymer hybrids are promising materials for biomedical applications because they combine the bioactivity of bioceramics with the flexibility of polymers. These advantages may be used in porous scaffolds for tissue-engineering applications. In previous works, hybrid foams bioactive glass/polyvinyl alcohol (PVA) were prepared by the sol-gel process. The hybrid foam 50% PVA/50% bioactive glass was chosen in the composition range studied as the scaffold with better compromise in terms of pore structure and mechanical behavior. The aim of the present study was to evaluate the adhesion, viability, and growth behavior of human-adipose-tissue-derived stem cells on bioactive glass/PVA foams in vitro and their potential as scaffold for application in bone-tissue engineering. The pore structure of the hybrid samples used in the study was analyzed by microcomputed tomography, showing a modal pore diameter of 284 μm and modal interconnect diameter of 138 μm. We found that cells are capable of adhesion, proliferation, growth, and ECM production on the scaffolds tested. The results show that the hybrid bioactive glass/PVA is a promising material for bone repair, providing a good environment for the adhesion and proliferation of human-adipose-tissue-derived stem cells in vitro.

scholarly journals Adipose-Derived Stem Cells for Future Regenerative System Medicine

2012 ◽  
Vol 4 (2) ◽  
pp. 59
Author(s):  
Yani Lina ◽  
Andi Wijaya
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Adult Stem Cells ◽  
Stromal Vascular Fraction ◽  
Human Adipose Tissue ◽  
Lytic Enzymes ◽  
Stromal Stem Cells

BACKGROUND: The potential use of stem cell-based therapies for repair and regeneration of various tissues and organs offers a paradigm shift that may provide alternative therapeutic solutions for a number of disease. Despite the advances, the availability of stem cells remaining a challenge for both scientist and clinicians in pursuing regenerative medicine. CONTENT: Subcutaneous human adipose tissue is an abundant and accessible cell source for applications in tissue engineering and regenerative medicine. Routinely, the adipose issue is digested with collagenase or related lytic enzymes to release a heterogeneous population for stromal vascular fraction (SVF) cells. The SVF cells can be used directly or can be cultured in plastic ware for selection and expansion of an adherent population known as adipose-derived stromal/stem cells (ASCs). Their potential in the ability to differentiate into adipogenic, osteogenic, chondrogenic and other mesenchymal lineages, as well in their other clinically useful properties, includes stimulation of angiogenesis and suppression of inflammation.SUMMARY: Adipose tissue is now recognized as an accessible, abundant and reliable site for the isolation of adult stem cels suitable for the application of tissue engineering and regenerative medicine applications. The past decade has witnessed an explosion of preclinical data relating to the isolation, characterization, cryopreservation, differentiation, and transplantation of freshly isolated stromal vascular fraction cells and adherent, culture-expanded, adipose-derived stromal/stem cells in vitro and in animal models.KEYWORDS: adipose tissue, adult stem cells, regenerative medicine, mesenchymal stem cells

Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo

Gut ◽  
2008 ◽  
Vol 58 (4) ◽  
pp. 570-581 ◽  
Author(s):  
H Aurich ◽  
M Sgodda ◽  
P Kaltwasser ◽  
M Vetter ◽  
A Weise ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Hepatocyte Differentiation ◽  
Adipose Tissue In Vitro

Comprehensive characterization of human adipose tissue-derived stem cells expanded in vitro

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Ľuboš Danišovič ◽  
Marcela Kuniaková ◽  
Zuzana Varchulová-Nováková ◽  
Martin Boháč ◽  
Ivan Varga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Growth Curve ◽  
Morphological Characteristics ◽  
Human Adipose Tissue ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Tem Analysis ◽  
Hla Dr

AbstractAdipose tissue seems to be a rich and safe source of mesenchymal stem cells (MSCs). The present study was aimed to investigate the biological and morphological characteristics of human adipose tissue-derived stem cells (ATSCs). Light and transmission electron microscopy were used. Course of proliferation was analyzed by growth curve. Expression of surface antigens was assessed by flow cytometry. Chondrogenic potential was assessed by immunohistochemistry. Obtained results showed morphology typical of fibroblastoid cells. TEM analysis proved ultrastructural morphology similar to MSCs from other sources. ATSCs reflected their proteosynthetic and metabolic activity. Each cell had irregular shape of nucleus with noticeable nucleoli. Abundant cisterns of rough endoplasmic reticulum were present in their cytoplasm. Karyotype mapping showed normal count of human chromosomes (46,XX). The growth curve revealed high capability for proliferation and population doubling time was 27.36 hours. ATSCs were positive for CD13, CD29, CD44, CD73, CD90, CD105 and CD106, but did not express CD14, CD34, CD45 and HLA-DR. It was also proved that ATSCs underwent chondrogenic differentiation in vitro. On the basis of obtained results it should be emphasized that ATSCs are typical MSCs and after further investigations they may be used in tissue engineering and regenerative medicine.

scholarly journals Comparison of the Effect of Sol-Gel and Coprecipitation Routes on the Properties and Behavior of Nanocomposite Chitosan-Bioactive Glass Membranes for Bone Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Elke M. F. Lemos ◽  
Sandhra M. Carvalho ◽  
Patrícia S. O. Patrício ◽  
Claudio L. Donnici ◽  
Marivalda M. Pereira
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Sol Gel ◽  
Composite Films ◽  
Maximum Tensile Stress ◽  
Control Group ◽  
Nanoparticle Dispersion

Recent studies in tissue engineering have highlighted the importance of the development of composite materials based on biodegradable polymers containing bioactive glasses, in particular, composites for high load support and excellent cell viability for potential application in bone regeneration. In this work, hybrid composite films were obtained by combining chitosan with bioactive glass in solution form and in nanoparticle dispersion form obtained by the two different synthesis routes: the sol-gel method and coprecipitation. The bioactive glass served both as a mechanical reinforcing agent and as a triggering agent with high bioactivity. The results ofin vitroassays with simulated body fluid demonstrated the formation of a significant layer of fibrils on the surface of the film, with a typical morphology of carbonated hydroxyapatite, reflecting induction of a favorable bioactivity. Maximum tensile stress increased from 42 to 80 MPa to the sample with 5% wt bioactive glass. In addition, samples containing 5% and 10% wt bioactive glass showed a significant increase in cell viability, 18 and 30% increase compared to the control group. The samples showed significant response, indicating that they could be a potential material for use in bone regeneration through tissue engineering.

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