scholarly journals Molecular Evidence for the Wide Distribution of Two Lineages of Terrestrial Green Algae (Chlorophyta) over Tropics to Temperate Zone

ISRN Ecology ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Ladislav Hodač ◽  
Christine Hallmann ◽  
Helen Rosenkranz ◽  
Fabian Faßhauer ◽  
Thomas Friedl
Keyword(s):  
Phylogenetic Analyses ◽  
Wide Distribution ◽  
Tree Bark ◽  
Close Relative ◽  
Molecular Evidence ◽  
European Origin ◽  
Rdna Sequences ◽  
Green Algal ◽  
18S Rdna Sequences ◽  
A New Species

Phylogenetic analyses of 18S rDNA sequences from environmental clones and culture strains revealed a widespread distribution of two subaerial green algal lineages, Jenufa and Xylochloris, recently described from rainforests in southeast Asia. A new lineage of Jenufa (Chlorophyceae), most closely related to or even conspecific with J. minuta, was formed by sequences of European origin. Two more lineages of Jenufa were formed by three additional sequences from Ecuador and Panama. The other lineage was a close relative of Xylochloris irregularis (Trebouxiophyceae), probably representing a new species of the genus and distinct from the only so far described species, X. irregularis. It comprised two distinct clades each containing almost identical sequences from Germany and Ecuador. Analyses of the new sequences for both genera allowed to presume a preference of J. minuta to subaerial growth on rock or artificial hard substrates combined with a remarkable adaptation to extended periods of darkness, whereas Xylochloris may preferably occur on tree bark or in the soil.

scholarly journals Choricystis and Lewiniosphaera gen. nov. (Trebouxiophyceae Chlorophyta), two different green algal endosymbionts in freshwater sponges

Symbiosis ◽  
2020 ◽  
Vol 82 (3) ◽  
pp. 175-188
Author(s):  
Thomas Pröschold ◽  
Tatyana Darienko
Keyword(s):  
Green Algae ◽  
Phylogenetic Analyses ◽  
Wide Distribution ◽  
Phylogenetic Position ◽  
Freshwater Sponges ◽  
Culture Collections ◽  
Rdna Sequences ◽  
Green Algal ◽  
Long Time ◽  
A New Species

Associations of freshwater sponges with coccoid green algae have been known for a long time. Two types of coccoid green algae, which are commonly assigned as zoochlorellae, are recognized by morphology: small coccoids (< 3 μm) without pyrenoids and larger Chlorella-like algae (4–6 μm) with pyrenoids. Despite their wide distribution in some freshwater sponges, these green algae were never studied using a combined analysis of morphology and molecular phylogeny. We investigated several endosymbiotic strains isolated from different Spongilla species, which were available in culture collections. Phylogenetic analyses of SSU and ITS rDNA sequences revealed that the strain SAG 211-40a is a member of the Chlorellaceae and represents a new species of the newly erected genus Lewiniosphaera, L symbiontica. The phylogenetic position was confirmed by morphology and ITS-2 barcode. The endosymbionts without pyrenoid were identified as Choricystis parasitica by morphology and phylogenetic analyses. The comparison with free-living strains revealed the recognition of two new Choricystis species, C. krienitzii and C. limnetica, which were confirmed by molecular signatures in V9 region of SSU rDNA and ITS-2 barcode.

Haemogregarines from western Palaearctic freshwater turtles (generaEmys, Mauremys) are conspecific withHaemogregarina stepanowiDanilewsky, 1885

Parasitology ◽  
2013 ◽  
Vol 141 (4) ◽  
pp. 522-530 ◽  
Author(s):  
NELA DVOŘÁKOVÁ ◽  
JANA KVIČEROVÁ ◽  
IVO PAPOUŠEK ◽  
HOSSEIN JAVANBAKHT ◽  
GHOULEM TIAR ◽  
...  
Keyword(s):  
Host Specificity ◽  
18S Rdna ◽  
Phylogenetic Analyses ◽  
Wide Distribution ◽  
Freshwater Turtles ◽  
Turtle Species ◽  
Rdna Sequences ◽  
Mauremys Leprosa ◽  
18S Rdna Sequences ◽  
Western Palaearctic

SUMMARYThe majority ofHaemogregarinaspecies have been based on the morphology of their erythrocytic stages and supposed strict host specificity. The quantity of species with a limited number of overlapping diagnostic traits has led to a considerable mess in haemogregarine taxonomy and significant synonymy. We analysed host specificity, intra- and interspecific variability, evolutionary relationships, and the distribution of the type species of the genusHaemogregarina–H. stepanowi.The morphology of blood stages and 18S rDNA sequences of this haemogregarine from four western Palaearctic hard-shelled freshwater turtles (Emys orbicularis, Mauremys caspica, Mauremys leprosaandMauremys rivulata) were compared withHaemogregarina balli. Additional sequences of 18S rDNA ofHaemogregarina-like isolates collected from three species of African hinged terrapins (genusPelusios) were used to enlarge the dataset for phylogenetic analyses. Thirteen sequences (1085 bp) ofHaemogregarinarepresenting all four western Palaearctic turtle species were identical, corresponding toH. stepanowi, which is closely related to the Nearctic speciesH. balli. In our analyses,Haemogregarinaspp. constituted a monophyletic clade sister to the genusHepatozoon. Haemogregarina stepanowipossesses a wide distribution range from the Maghreb, through Europe, Turkey and the Middle East to Iran. We consider that the genusHaemogregarinahas a low host specificity crossing the family level of its vertebrate hosts and that its distribution is likely to be linked to the vector and definitive host – the leech.

New insights on Trametopsis Tomšovský (Polyporales Gäum) based on phylogenetic evidences and morphological analyses of neotropical species

Phytotaxa ◽  
2017 ◽  
Vol 311 (2) ◽  
pp. 155 ◽  
Author(s):  
NATALY GÓMEZ-MONTOYA ◽  
ELISANDRO RICARDO DRECHSLER-SANTOS ◽  
VALÉRIA FERREIRA-LOPES ◽  
MICHAL TOMŠOVSKÝ ◽  
CARLOS URCELAY ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Lsu Rdna ◽  
Molecular Evidence ◽  
Type Specimens ◽  
Morphological Pattern ◽  
Neotropical Species ◽  
Rdna Sequences ◽  
Molecular Phylogenetic ◽  
A New Species

Based on molecular evidence and morphological analyses, a new species in the genus Trametopsis is revealed. Trametopsis aborigena sp. nov. is proposed and described from South America. Molecular phylogenetic analyses of ITS and LSU rDNA sequences suggest that Trametopsis cervina seems to encompass more than one species and confirm previous results showing that Antrodiella brasiliensis groups with Trametopsis. The morphological analyses of type specimens of A. brasiliensis and A. luteocontexta revealed a shared morphological pattern with Trametopsis and their combinations in Trametopsis are proposed. The circumscription of Trametopsis is discussed since all the studied specimens revealed a previously overlooked arrangement of characters, i.e., cylindrical and slightly curved to allantoid basidiospores and a metachromatic hyphal system monomitic in the context and dimitic in the tubes.

scholarly journals Unexplored diversity of microscopic myxomycetes: evidence from environmental DNA

2019 ◽  
Vol 152 (3) ◽  
pp. 499-506 ◽  
Author(s):  
Oleg N. Shchepin ◽  
Martin Schnittler ◽  
Nikki H.A. Dagamac ◽  
Dmitry V. Leontyev ◽  
Yuri K. Novozhilov
Keyword(s):  
New Species ◽  
18S Rdna ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Environmental Dna ◽  
Full Length ◽  
Detection Techniques ◽  
Rdna Sequences ◽  
18S Rdna Sequences ◽  
Environmental Sequences

Background and aims – Recent studies showed the position of two slime mould species with microscopic sporocarps, Echinosteliopsis oligospora and Echinostelium bisporum, within the class Myxomycetes. These minute species are seldom seen in studies based on detection of sporocarps and can easily be confused with protosteloid amoebozoans.Methods – We searched all published ePCR data sets that targeted myxomycete 18S rDNA for the presence of environmental sequences similar to E. oligospora and Echinosteliales in traditional circumscription, and performed phylogenetic analyses that included short environmental sequences and full-length 18S rDNA sequences representing all the major groups of myxomycetes.Key results – We report 19 unique sequences which are closely related to E. bisporum or E. oligospora based on sequence similarity (73.1–95.2% similarity) and which form well-supported monophyletic clades with these species in phylogenetic analyses. They may represent new species that are not yet described. Our phylogeny based on full-length 18S rDNA sequences further confirms the position of E. bisporum and E. oligospora within myxomycetes and the paraphyly of the order Echinosteliales in its traditional circumscription.Conclusions – Our results show that ePCR-based studies can reveal myxomycete taxa that often escape detection by traditional approaches, including potentially new species, and thus provide valuable new data on diversity and ecology of myxomycetes. As such, strategies for studying myxomycetes biodiversity should be revised, focusing also on molecular detection techniques in addition to the sporocarp-based ones.

Strobilomyces rubrobrunneus (Boletaceae), a new species with reddish brown scales from eastern China

Phytotaxa ◽  
2018 ◽  
Vol 376 (4) ◽  
pp. 167
Author(s):  
LI-HONG HAN ◽  
YAN-JIA HAO ◽  
CHAO LIU ◽  
DONG-QIN DAI ◽  
KUAN ZHAO ◽  
...  
Keyword(s):  
New Species ◽  
Related Species ◽  
Phylogenetic Analyses ◽  
Eastern China ◽  
Mixed Forest ◽  
Molecular Evidence ◽  
Gene Markers ◽  
Black Discoloration ◽  
A New Species

A new species, Strobilomyces rubrobrunneus, is described from eastern China based on morphological and molecular evidence. Morphologically, this species is characterized by its tiny to small basidiomes, reddish-brown and more or less erect pyramidal scales, grayish black discoloration of the pileal context when bruised, reticulate spores and a subtropical distribution in a mixed forest of Fagaceae and Pinaceae. Our phylogenetic analyses based on four gene markers (rpb1, rpb2, tef1 and cox3) support its placement in Strobilomyces and its separation from other related species of this genus. Morphological illustrations and description are provided.

Morphological and molecular evidence for a new species of the genus Panolis Hübner, [1821] (Lepidoptera: Noctuidae)

Zootaxa ◽  
2019 ◽  
Vol 4565 (4) ◽  
pp. 523
Author(s):  
XUAN AN ◽  
MAMORU OWADA ◽  
MIN WANG ◽  
HOU-SHUAI WANG
Keyword(s):  
New Species ◽  
Cytochrome C Oxidase ◽  
Phylogenetic Analyses ◽  
Species Group ◽  
Molecular Evidence ◽  
Mitochondrial Cytochrome ◽  
Molecular Phylogenetic ◽  
Lepidoptera Noctuidae ◽  
A New Species ◽  
I Gene

A new species of the genus Panolis Hübner, [1821], P. xundian sp. nov., is described and illustrated from southwestern China. It is well-defined morphologically by the male genitalia, with a well-developed pollex at the distal terminal of the sacculus and a broad, ventrally concave cucullus, the female corpus bursae with four long signum-stripes. Based on a 658 bp segment of the mitochondrial cytochrome c oxidase subunit I gene, we report the pairwise genetic distance of 2.5% from its allied species P. exquisita Draudt, 1950. Molecular phylogenetic analyses using three genes (2189 bp in total length) indicate that the new species belongs to the P. exquisita species group. 

Recognition of a new species of Hedysarum (Fabaceae, Hedysareae) from China based on morphological and molecular evidence

Phytotaxa ◽  
2017 ◽  
Vol 295 (3) ◽  
pp. 237 ◽  
Author(s):  
PEI-LIANG LIU ◽  
YING WEI ◽  
JUN WEN ◽  
ZHAO-YANG CHANG
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Eastern Himalaya ◽  
Molecular Evidence ◽  
Calyx Tube ◽  
Calyx Tooth ◽  
Nuclear Its ◽  
A New Species

A new species, Hedysarum cuonanum (Fabaceae, Hedysareae), is described from the eastern Himalaya in China. This new species is similar to H. xizangense, but differs by its lanceolate or narrowly elliptic leaflet blade, ovate or lanceolate bracteole about half the length of calyx tube, acute or obtuse standard apex, and mucronate keel apex. It also resembles H. longigynophorum, but can be distinguished by its leaflet blade with 13–19 secondary veins on each side, abaxial calyx tooth about 1.5 times as long as the adaxial teeth, standard as long as keel, and elliptic or ovate loment article. Phylogenetic analyses based on the nuclear ITS, ETS sequences, and the plastid psbA-trnH, trnC-petN, petN-psbM sequences showed that H. cuonanum is sister to H. xizangense, and the H. cuonanum + H. xizangense clade is then sister to H. longigynophorum.

Two new species and two new records of myxomycetes from subtropical forests in China

Phytotaxa ◽  
2018 ◽  
Vol 350 (1) ◽  
pp. 51 ◽  
Author(s):  
YANG GAO ◽  
SHU-ZHEN YAN ◽  
GAO-WEI WANG ◽  
SHUANG-LIN CHEN
Keyword(s):  
New Species ◽  
New Records ◽  
Phylogenetic Analyses ◽  
Diagnostic Feature ◽  
Subtropical Forests ◽  
Rdna Sequences ◽  
Two New Species ◽  
18S Rdna Sequences ◽  
First Time ◽  
Partial 18S

Two new myxomycetes from subtropical forests of China, Arcyria aeruginosa sp. nov. and Diderma subochraceum sp. nov., are described and illustrated. Arcyria aeruginosa occurred on rotten stem of bamboo and differs from all known species of the genus in having dense spines on inner surface of cups. The diagnostic feature of D. subochraceum is single-layered peridium which dehisces by longitudinal or stellate slit. Preliminary phylogenetic analyses based on partial 18S rDNA sequences support the establishment of the two new species. Additionally, Didymium saturnus H.W. Keller and Fuligo luteonitens L.G. Krieglst. & Nowotny are reported from China for the first time.

Molecular data implicate bryozoans as hosts for PKX (Phylum Myxozoa) and identify a clade of bryozoan parasites within the Myxozoa

Parasitology ◽  
1999 ◽  
Vol 119 (6) ◽  
pp. 555-561 ◽  
Author(s):  
C. L. ANDERSON ◽  
E. U. CANNING ◽  
B. OKAMURA
Keyword(s):  
18S Rdna ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Salmonid Fish ◽  
Abnormal Immune Response ◽  
Rdna Sequences ◽  
Pectinatella Magnifica ◽  
18S Rdna Sequences ◽  
Cristatella Mucedo ◽  
Distinct Taxon

Proliferative kidney disease (PKD), a condition associated with high mortality in salmonid fish, represents an abnormal immune response to the presence of an enigmatic myxozoan, which has been designated simply as PKX organism because its generic and specific status are obscure. Phylogenetic analyses of partial sequences of the 18S rDNA of PKX and of myxozoan parasites infecting the bryozoans Cristatella mucedo, Pectinatella magnifica and Plumatella rugosa, including the previously named Tetracapsula bryozoides from C. mucedo, showed that these taxa represent a distinct clade that diverged early in the evolution of the Myxozoa before the radiation of the other known myxozoan genera. A common feature of the myxozoans in this clade may be the electron-dense sporoplasmosomes with a lucent bar-like structure, which occur in T. bryozoides and PKX but not in the myxozoans belonging to the established orders Bivalvulida and Multivalvulida. Variation of 0·5–1·1% was found among the PKX 18S rDNA sequences obtained from fish from North America and Europe. The 18S rDNA sequence for T. bryozoides showed that it is a distinct taxon, not closely related to PKX but some sequences from myxozoans infecting 2 of the bryozoan species were so similar to those of PKX as to be indistinguishable. Other sequences from the new myxozoans in bryozoans at first appeared distinct from PKX in a maximum likelihood tree but, when analysed further, were also found to be phylogenetically indistinguishable from PKX. We propose that at least some variants of these new myxozoans from bryozoans are able to infect and multiply in salmonid fish, in which they stimulate the immune reaction and cause PKD but are unable to form mature spores to complete their development.

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