scholarly journals Isolation and Molecular Characterization of Fowl Adenoviruses Associated with Inclusion Body Hepatitis-hydropericardium Syndrome in Broiler Chickens in Egypt

2021 ◽  
Vol 11 (3) ◽  
pp. 333-342
Author(s):  
Mostafa G. Al Naguib ◽  
Eman A. Morsy ◽  
Mahmoud Samir ◽  
Marwa S. Khattab ◽  
Diaa G. Khelfa ◽  
...  
Keyword(s):  
Inclusion Body ◽  
Broiler Chickens ◽  
Conventional Polymerase Chain Reaction ◽  
Economic Losses ◽  
Poultry Production ◽  
Hydropericardium Syndrome ◽  
Type D ◽  
Inclusion Body Hepatitis ◽  
Pcr Products ◽  
Pcr Targeting

Avian adenoviruses are an extremely diversified group of pathogens that recently triggering a variety of problems for poultry production. In particular, Inclusion Body Hepatitis-Hydropericardium Syndrome (IBH-HPS), which has been observed in broiler birds from 3 to 6 weeks of age and is associated with anemia, hemorrhagic disorders, hydropericardium, and high mortality. The disease has been reported worldwide, and recently it was reported in many Egyptian farms, causing severe economic losses. Therefore, the current study aimed to isolate, and genetically type the most common Adenovirus serotypes associated with this syndrome in Egyptian farms. A total of 50 broiler chicken farms (3-6 weeks old) located in different Egyptian governorates were examined. Macroscopically, the diseased flock revealed hydropericardium, enlarged friable livers with ecchymotic hemorrhages, and varying mortality rates (1 to 7.5%). Histopathologically, severe diffuse necrotizing enteritis, hepatitis, pericarditis, and diffuse lymphoid depletion of the spleen were the most prominent lesions. Liver tissues and cloacal swabs were collected from all examined flocks for FAdVs detection by conventional polymerase chain reaction (PCR) targeting the L1 loop in the hexon gene. The PCR products were sequenced for typing of the detected viruses. It was found that 10 out of 50 flocks examined were PCR positive for FAdVs (20%). Phylogenetic analysis of the sequenced genes revealed that the obtained viruses clustered with reference strains belonging to FAdV type D and E serotype 2, 11, and 8a respectively. The isolation of both FAdV type D and FAdV type E were carried out on a primary cell culture chicken embryo liver cell (CEL) and the presence of these viruses was confirmed by PCR after the appearance of cytopathic effect (CPE). From this study, it could be concluded that both FAdVs types D and E are the most common adenoviruses circulating in poultry farms suffering from hydropericardium and inclusion body hepatitis.

scholarly journals Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens

VirusDisease ◽  
2013 ◽  
Vol 25 (1) ◽  
pp. 114-119 ◽  
Author(s):  
Dinesh Mittal ◽  
Naresh Jindal ◽  
Ashok Kumar Tiwari ◽  
Raj Singh Khokhar
Keyword(s):  
Inclusion Body ◽  
Broiler Chickens ◽  
Hydropericardium Syndrome ◽  
Inclusion Body Hepatitis

scholarly journals Molecular and Pathotypic Characterization of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Indian Chickens

2020 ◽  
Author(s):  
Dasharath B. Shinde ◽  
Anil L Thormoth ◽  
Santosh S. Koratkar ◽  
Neeti Sharma ◽  
Ashok Rajguru ◽  
...  
Keyword(s):  
Inclusion Body ◽  
Broiler Chickens ◽  
Emerging Diseases ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Hydropericardium Syndrome ◽  
Inclusion Body Hepatitis ◽  
Infective Dose ◽  
Pathogenic Analysis

Abstract Inclusion body hepatitis (IBH) is a disease of chickens characterized by acute mortality with severe anemia caused by fowl adenoviruses (FAdV). IBH is one of the most important re-emerging diseases world-wide. In India earlier IBH/ Hydropericardium Syndrome (HPS) outbreaks were mainly due to FAdV) type 4; however, in recent outbreaks, other serotypes are involved, despite regular vaccination in breeders and young chicks. The aim of the present study was isolation and molecular characterization of FAdV associated with IBH in India. A total of 193 liver samples of IBH suspected broiler chickens were collected from different regions of India. Liver samples were initially screened by histopathology and further, a total of 127 samples which microscopically showed basophilic, intra-nuclear inclusion bodies were processed for FAdV detection. A total of 69 samples were found to be positive for Hexon gene in PCR, of which 28 were sequenced. These samples showed more than 94% sequence homology with FAdV2 and 97% with FAdV11, which was confirmed by Restriction Enzyme Analysis (REA). Tissue Culture Infective Dose 50 (TCID50) and Egg Infective Dose50 (EID50) titer of isolated FAdV was found to be 106.5/ml. Pathogenicity study in SPF chicks showed 100% mortality up to Post Infection Day (PID)-6. These findings conclude that the prevalence of FAdV-11 is currently causing IBH outbreaks India. However, further genetic and pathogenic analysis of FAdV is required, which would provide useful information for the development of the efficient commercial IBH vaccine.

scholarly journals Pretreatment with probiotics ameliorate gut health and necrotic enteritis in broiler chickens, a substitute to antibiotics

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Danish Sharafat Rajput ◽  
Dong Zeng ◽  
Abdul Khalique ◽  
Samia Sharafat Rajput ◽  
Hesong Wang ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Production Systems ◽  
Economic Losses ◽  
Necrotic Enteritis ◽  
Poultry Production ◽  
Gut Health ◽  
Growth Promoters ◽  
Broiler Performance ◽  
Antibiotic Growth Promoters ◽  
Microbial Strains

AbstractNecrotic enteritis (NE) is being considered as one of the most important intestinal diseases in the recent poultry production systems, which causes huge economic losses globally. NE is caused by Clostridium perfringens, a pathogenic bacterium, and normal resident of the intestinal microflora of healthy broiler chickens. Gastrointestinal tract (GIT) of broiler chicken is considered as the most integral part of pathogen’s entrance, their production and disease prevention. Interaction between C. perfringens and other pathogens such as Escherichia coli and Salmonella present in the small intestine may contribute to the development of NE in broiler chickens. The antibiotic therapy was used to treat the NE; however European Union has imposed a strict ban due to the negative implications of drug resistance. Moreover, antibiotic growth promoters cause adverse effects on human health as results of withdrawal of antibiotic residues in the chicken meat. After restriction on use of antibiotics, numerous studies have been carried out to investigate the alternatives to antibiotics for controlling NE. Thus, possible alternatives to prevent NE are bio-therapeutic agents (Probiotics), prebiotics, organic acids and essential oils which help in nutrients digestion, immunity enhancement and overall broiler performance. Recently, probiotics are extensively used alternatives to antibiotics for improving host health status and making them efficient in production. The aim of review is to describe a replacement to antibiotics by using different microbial strains as probiotics such as bacteria and yeasts etc. having bacteriostatic properties which inhibit growth of pathogens and neutralize the toxins by different modes of action.

scholarly journals Molecular mechanisms of growth depression in broiler chickens (Gallus Gallus domesticus) mediated by immune stress: a hepatic proteome study

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Aijuan Zheng ◽  
Anrong Zhang ◽  
Zhimin Chen ◽  
Shoaib Ahmed Pirzado ◽  
Wenhuan Chang ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Molecular Mechanisms ◽  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Economic Losses ◽  
Poultry Production ◽  
Gallus Gallus Domesticus ◽  
Growth Depression ◽  
Stress Group ◽  
Immune Stress

Abstract Background Immunological stress decreases feed intake, suppresses growth and induces economic losses. However, the underlying molecular mechanism remains unclear. Label-free liquid chromatography and mass spectrometry (LC-MS) proteomics techniques were employed to investigate effects of immune stress on the hepatic proteome changes of Arbor Acres broilers (Gallus Gallus domesticus) challenged with Escherichia coli lipopolysaccharide (LPS). Results Proteomic analysis indicated that 111 proteins were differentially expressed in the liver of broiler chickens from the immune stress group. Of these, 28 proteins were down-regulated, and 83 proteins were up-regulated in the immune stress group. Enrichment analysis showed that immune stress upregulated the expression of hepatic proteins involved in defense function, amino acid catabolism, ion transport, wound healing, and hormone secretion. Furthermore, immune stress increased valine, leucine and isoleucine degradation pathways. Conclusion The data suggests that growth depression of broiler chickens induced by immune stress is triggered by hepatic proteome alterations, and provides a new insight into the mechanism by which immune challenge impairs poultry production.

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