Phylogenetic Analyses of Pepper mild mottle virus and Cucumber mosaic virus Isolated from Rorippa palustris

Cucumber mosaic virus (CMV) and Pepper mild mottle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material – chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS and PCS (0.01%, 0.05%, and 0.1% concentration) compound against PMMoV infection and replication was tested using a half-leaf assay on Nicotiana glutinosa leaves. The total number of local lesions represented on a leaf of N. glutinosa were counted and analyzed with phosphate buffer treated leaves as a negative control. The leaves treated with a 0.1% concentration of CS or PCS compounds exhibited an inhibition effect by 40-75% compared with the control leaves. The same treatment significantly reduced about 40% CMV accumulation measured by double antibody sandwich enzyme-linked immunosorbent assay and increased the relative expression levels of the NPR1, PR-1, cysteine protease inhibitor gene, LOX, PAL, SRC2, CRF3 and ERF4 genes analyzed by quantitative reverse transcriptase-polymerase chain reaction, in chili pepper plants.

Download Full-text

Evolutionary History of Cucumber Mosaic Virus Deduced by Phylogenetic Analyses

Journal of Virology ◽

10.1128/jvi.76.7.3382-3387.2002 ◽

2002 ◽

Vol 76 (7) ◽

pp. 3382-3387 ◽

Cited By ~ 155

Author(s):

Marilyn J. Roossinck

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Virus Isolate ◽

Phylogenetic Analyses ◽

Open Reading Frames ◽

Broad Host Range ◽

Nontranslated Region ◽

History Of ◽

Evolutionary Success ◽

Reading Frames

ABSTRACT Cucumber mosaic virus (CMV) is an RNA plant virus with a tripartite genome and an extremely broad host range. Previous evolutionary analyses with the coat protein (CP) and 5′ nontranslated region (NTR) of RNA 3 suggested subdivision of the virus into three groups, subgroups IA, IB, and II. In this study 15 strains of CMV whose nucleotide sequences have been determined were used for a complete phylogenetic analysis of the virus. The trees estimated for open reading frames (ORFs) located on the different RNAs were not congruent and did not completely support the subgrouping indicated by the CP ORF, indicating that different RNAs had independent evolutionary histories. This is consistent with a reassortment mechanism playing an important role in the evolution of the virus. The evolutionary trees of the 1a and 3a ORFs were more compact and displayed more branching than did those of the 2a and CP ORFs. This may reflect more rigid host-interactive constraints exerted on the 1a and 3a ORFs. In addition, analysis of the 3′ NTR that is conserved among all RNAs indicated that evolutionary constraints on this region are specific to the RNA component rather than the virus isolate. This indicates that functions other than replication are encoded in the 3′ NTR. Reassortment may have led to the genetic diversity found among CMV strains and contributed to its enormous evolutionary success.

Download Full-text

Infeksi Cucumber Mosaic Virus dan Chili Veinal Mottle Virus terhadap Pertumbuhan dan Hasil Tanaman Cabai

HAYATI Journal of Biosciences ◽

10.1016/s1978-3019(16)30381-3 ◽

2006 ◽

Vol 13 (2) ◽

pp. 53-57 ◽

Cited By ~ 1

Author(s):

DWI SUBEKTI ◽

SRI HENDRASTUTI HIDAYAT ◽

ENDANG NURHAYATI ◽

SRIANI SUJIPRIHATI

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Mottle Virus ◽

Chili Veinal Mottle Virus

Download Full-text

Detection and molecular characterization of Pepper mild mottle virus in Serbia

Genetika ◽

10.2298/gensr1502651m ◽

2015 ◽

Vol 47 (2) ◽

pp. 651-663 ◽

Cited By ~ 3

Author(s):

Dragana Milosevic ◽

Ivana Stankovic ◽

Aleksandra Bulajic ◽

Maja Ignjatov ◽

Zorica Nikolic ◽

...

Keyword(s):

Mosaic Virus ◽

Molecular Characterization ◽

Great Influence ◽

Alfalfa Mosaic Virus ◽

Resistance Breeding ◽

Elisa Test ◽

Mottle Virus ◽

Pepper Mild Mottle Virus ◽

Das Elisa

During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P1,2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.

Download Full-text

KETAHANAN BEBERAPA KULTIVAR CABAI TERHADAP CUCUMBER MOSAIC VIRUS DAN CHILLI VEINAL MOTTLE VIRUS

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.27130-139 ◽

2012 ◽

Vol 7 (2) ◽

pp. 130-139

Author(s):

Muhammad Taufik ◽

Sri Hendrastuti Hidayat ◽

Sriani Sujiprihati ◽

Gede Suastika ◽

Sientje Mandang Sumaraw

Keyword(s):

Virus Infection ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Yield Loss ◽

Disease Incidence ◽

Growth And Yield ◽

Mottle Virus ◽

Tolerance Response ◽

Chilli Veinal Mottle Virus ◽

Das Elisa

Resistance Evaluation of Chillipepper Cultivars for Cucumber Mosaic Virus and Chilli Veinal Mottle Virus. The use of resistance culivars is an important strategy for management of virus infection in chillipepper. A research was undergone to study the effect of single and mix infection of CMV and ChiVMV on the disease incidence and on the growth and yield of nine chillipepper cultivars, i.e. Cilibangi 4, Cilibangi 5, Cilibangi 6, Helem, Jatilaba, Tit Bulat, Tit Segitiga, Tit Super and Tampar. Mechanical inoculation was conducted to transmit the virus. Infection of the virus was then confirmed with DAS-ELISA. In general, inoculated chillipepper cultivars developed similar symptoms, i.e. mosaic type for CMV and mottle type for ChiVMV. More severe symptom was not always observed from mix infection of CMV and ChiVMV. Disease incidence occurred in the range of 16.67 – 86.0% and this caused 18.3 – 98.6% yield loss. Based on symptom expression, ELISA result, and reduction on yield, it can be concluded that all chillipepper cultivars used in this study could not hold up the virus infection. However, several cultivars showed tolerance response : Jatilaba, Tit Super, and Tampar for CMV; Cilibangi 4 for ChiVMV; Tit Super for mix infection; and Cilibangi 5 for CMV, ChiVMV, and mix infection. Further evaluation and investigation involving different chillipepper cultivars should be conducted.

Download Full-text