Phylogenetic analysis and spatial distribution of bovine viral diarrhea virus (BVDV) in dairy cattle from Galicia (NW Spain)

Aim of study: To examine the frequency and diversity of bovine viral diarrhea viruses infecting dairy cattleArea of study: The study was carried out in Galicia (NW Spain), the main dairy cattle area of SpainMaterial and methods: A total of 157 BVDV isolates (from 140 dairy herds) were typed. Typing was based on a 288-bp sequence from the 5′ untranslated region of viral RNA genome. Subsequently, to investigate whether the presence of herds diagnosed with a particular strain was higher in some areas or during some specific time period, data were tested using a Bernouille approachMain results: Of the 157 isolates, 137 (87.3%) were typed as BVDV-1b, 10 (6.4%%) as 1d, 7 (4.4%) as 1e and 2 (1.3%) as 1f. One isolate was assigned to type 1p. Three of the strains found in the study (the three belonging to type 1b) showed significant spatial clustering.Research highlights: This report indicates that BVDV-1b was the predominant species, although there was an important genetic diversity in the study population. Spatial analysis indicated important drawbacks in the application of biosecurity measures, especially as regards purchase of cattle or after the reintroduction of animals from cattle concentration points.

Download Full-text

Prevalence of bovines persistently infected with bovine viral diarrhea virus (BVDV) in dairy cattle herds in Paraná State, Brazil

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-6622 ◽

2021 ◽

Vol 41 ◽

Author(s):

Bárbara B. Freitas ◽

Avelino M.F. Correa ◽

Altair A. Valotto ◽

Nicoly N. Marcom ◽

Laís R. Paulino ◽

...

Keyword(s):

Dairy Cattle ◽

Bovine Viral Diarrhea Virus ◽

Elisa Test ◽

Farm Animals ◽

Bovine Viral Diarrhea ◽

Dairy Herds ◽

Persistently Infected ◽

Diarrhea Virus ◽

Viral Diarrhea ◽

Viral Diarrhea Virus

ABSTRACT: This study aimed to establish the prevalence of animals persistently infected (PI) with bovine viral diarrhea virus (BVDV) in dairy farms at Parana State, Brazil. Samples were collected from 6,465 female Holstein Friesian Dairy Cattle, including animals less than two years old, females over two years old who had not given birth at the farm, and mothers of calves diagnosed as persistently infected. The cattle came from 40 dairy herds distributed in 10 municipalities in the State of Paraná. The samples were obtained from May 2015 to August 2018. The diagnosis of PI animals was made with an antigen-capture ELISA test. We detected PI animals in fifteen herds sampled (37.5%), ranging from one to sixteen animals per herd. The prevalence in Parana State’s municipalities was 1.78%, ranging from 0.3 to 8.9% at positive herds. The analysis of the individual herds shows significant dissemination of the BVDV in Parana’s municipalities, including endemic areas. With this, we highlight the need for measures to raise awareness among producers about the existence and importance of bovine viral diarrhea (BVD) in dairy herds, reinforcing the PI animals’ role in disease epidemiology and the economic impact caused by the maintenance of these farm animals.

Download Full-text