Non albicans Candida species: its isolation pattern, species distribution, virulence factors and antifungal susceptibility profile

Background and Objectives: Cutaneous candidiasis is a multipicture fungal infection caused by members of the genus Candida which is considered as a public health problem all over the world with urgency of effective treatment and control. This study was performed to analyze the clinical epidemiology and molecular aspects of cutaneous candidiasis in Tehran-Iran in relation to antifungal susceptibility and virulence factors of etiologic Candida species. Materials and Methods: Candida species were isolated from skin (27.3%) and nail scrapings (72.7%) of suspected patients and identified by ITS sequencing. Phylogeny of the isolates was evaluated using multilocus sequence typing (MLST) and antifungal susceptibility and virulence factors of the isolates were determined in relation to clinical presentation. Results: Candida albicans was the most prevalent species (39.8%), followed by C. parapsilosis (32.9%), C. orthopsilosis (10.4%), C. tropicalis (7.9%), C. glabrata and C. guilliermondii, each (4.5%). Molecular typing of 35 C. albicans isolates by MLST revealed 28 novel sequence types with 11 singletons with 80.0% new diploid sequence types (DSTs). Majority of the isolates were susceptible to amphotericin B (91.5%), followed by posaconazole (90.3%), fluconazole (84.3%), itraconazole (74.1%), caspofungin (53.6%), and voriconazole (26.8%). Biofilm formation, yeast-to-hyphae transformation and phospho- lipase activity were reported species-dependent. Conclusion: Our results demonstrated clinical epidemiology of various Candida species from cutaneous candidiasis dis- tributed in new molecular types with increasing importance of drug resistant of non-albicans Candida species. Our results showed that drug susceptibility and genetic variability of Candida species may be attributed to their clinical features and source of isolation.

Download Full-text

Species distribution of Candida species isolated from sterile body fluids and its antifungal susceptibility pattern in UMMC

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2020.09.1023 ◽

2020 ◽

Vol 101 ◽

pp. 390-391

Author(s):

J. Abd Aziz ◽

N. Atiya ◽

R.D. Velayuthan

Keyword(s):

Species Distribution ◽

Candida Species ◽

Antifungal Susceptibility ◽

Body Fluids ◽

Susceptibility Pattern ◽

Sterile Body Fluids

Download Full-text

Species distribution of yeasts recovered from different clinical samples Addiss Ababa,Ethiopia

10.21203/rs.2.9212/v2 ◽

2019 ◽

Author(s):

Elias Seyoum Deribe ◽

Adane Bitew ◽

Amete Mihret

Keyword(s):

Species Distribution ◽

Candida Species ◽

Fungal Infections ◽

Addis Ababa ◽

Antifungal Susceptibility ◽

Signs And Symptoms ◽

Cross Sectional Study ◽

Clinical Samples ◽

Cross Sectional ◽

Reported Data

Abstract Background Currently, fungal diseases of humans are among the most problematic illnesses is the result of an increase in opportunistic fungal infections caused by rise in the number of immune-compromised patients. Particularly, yeasts are the most common cause of fungal infections leading to a range superficial type to life-threatening invasive infections. The aim of this study is to determine species distribution of yeasts particularly of Candida species recovered from different clinical samples. Method A cross sectional study was conducted from January 01, 2018 –September 30, 2018 in Addis Ababa, Arsho laboratory. Oropharengial swabs, nail scrapings, sputum, corneal scrapings, and vaginal swabs were collected from patients with signs and symptoms of infections. Identification and antifungal susceptibility testing was performed by using YST card through VITEK 2 compact system. Data was entered and analyzed using SPSS version 20. Result A total of 209 yeasts were recovered of which 49.8% were Candida albicans, 43.1% were non-albicans candida species and 7.2% were yeasts other than Candida species. The current study, eighteen (18) different types of yeast species were identified from different clinical samples. All recognized yeast considered as a causative agent of fungal infection in previously reported data in worldwide. Conclusion C. albicans remaining the predominant species, but the emergence of non-albicans Candida and other than candida species have increased. Thus, more studies on Candida prevalence are needed throughout country.

Download Full-text

Species distribution of yeasts recovered from different clinical samples Addiss Ababa,Ethiopia

10.21203/rs.2.9212/v1 ◽

2019 ◽

Author(s):

Elias Seyoum Deribe ◽

Adane Bitew ◽

Amete Mihret

Keyword(s):

Species Distribution ◽

Candida Species ◽

Fungal Infections ◽

Addis Ababa ◽

Antifungal Susceptibility ◽

Signs And Symptoms ◽

Cross Sectional Study ◽

Clinical Samples ◽

Cross Sectional ◽

Reported Data

Abstract Background Currently, fungal diseases of humans are among the most problematic illnesses is the result of an increase in opportunistic fungal infections caused by rise in the number of immune-compromised patients. Particularly, yeasts are the most common cause of fungal infections leading to a range superficial type to life-threatening invasive infections. The aim of this study is to determine species distribution of yeasts particularly of Candida species recovered from different clinical samples. Method A cross sectional study was conducted from January 01, 2018 –September 30, 2018 in Addis Ababa, Arsho laboratory. Oropharengial swabs, nail scrapings, sputum, corneal scrapings, and vaginal swabs were collected from patients with signs and symptoms of infections. Identification and antifungal susceptibility testing was performed by using YST card through VITEK 2 compact system. Data was entered and analyzed using SPSS version 20. Result A total of 209 yeasts were recovered of which 49.8% were Candida albicans, 43.1% were non-albicans candida species and 7.2% were yeasts other than Candida species. The current study, eighteen (18) different types of yeast species were identified from different clinical samples. All recognized yeast considered as a causative agent of fungal infection in previously reported data in worldwide. Conclusion C. albicans remaining the predominant species, but the emergence of non-albicans Candida and other than candida species have increased. Thus, more studies on Candida prevalence are needed throughout country.

Download Full-text