Bioinformatics analysis of structures and ligand-bindings of predicted zymogen granule protein observed on Bali cattle (Bos javanicus) saliva

Two experiments were conducted with weaned entire male Bali cattle (Bos javanicus) between 6 and 12 months of age. A randomised block design was used in both experiments, which consisted of four treatments (diets), with five replicates (animals) per treatment. In both experiments, the average daily liveweight gain (LWG), feed and water intake and diet digestibility were determined over 8 or 10 weeks, with chest girth and wither height measured at the commencement and end of the experiments. In Experiment 1, the treatments were fresh native grass, native grass supplemented with fresh sesbania, fresh sesbania and fresh sesbania supplemented with rice bran. In Experiment 2, the treatments were sole diets of leucaena hay, sesbania hay, moringa hay or gliricidia hay supplemented with sesbania hay. In Experiment 1, animals fed sesbania supplemented with rice bran had higher DM intake than did animals fed the other three diets. DM digestibility (DMD) was significantly lower for weaners fed the native grass diet than for those fed the other diets. Animals fed sesbania or sesbania supplemented with rice bran had a higher LWG (0.34 and 0.43 kg/day, respectively) and estimated metabolisable energy (ME) intake (0.71 and 0.80 MJ of ME/kg W0.75.day, respectively) than did animals fed native grass alone (0.03 kg/day; 0.58 MJ of ME/kg W0.75.day) or native grass supplemented with sesbania (0.07 kg/day; 0.64 MJ of ME/kg W0.75.day). In Experiment 2, animals fed gliricidia hay had the lowest DM intake. DM intake of animals fed moringa hay was higher than that of animals fed gliricidia hay, but lower than the intake of animals fed sesbania hay or leucaena hay. DMD of the sesbania and leucaena hay diets was lower than that of the gliricidia and moringa hay diets. Animals fed leucaena and sesbania hays had a higher LWG (0.47 and 0.43 kg/day, respectively) and estimated ME intake (0.75 and 0.78 MJ of ME/kg W0.75.day, respectively) than did animals fed moringa hay (0.22 kg/day; 0.66 MJ ME/kg W0.75.day) or gliricidia hay supplemented with sesbania hay (0.0 kg/day; 0.48 MJ ME/kg W0.75.day). In conclusion, tree legumes can safely be fed to growing Bali cattle as the sole component of the diet and some of them will increase LWGs above that of native grasses, which are typically fed to Bali cattle in villages.

Download Full-text

Molecular architecture of the pancreatic zymogen granule-protein copy number determination

Pancreatology ◽

10.1016/j.pan.2012.12.314 ◽

2013 ◽

Vol 13 (2) ◽

pp. e74

Author(s):

P. Skallos ◽

G. Hubbs ◽

J. Fang ◽

P. Stemmer ◽

B. Jena ◽

...

Keyword(s):

Copy Number ◽

Zymogen Granule ◽

Molecular Architecture ◽

Granule Protein ◽

Copy Number Determination

Download Full-text

Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

HAYATI Journal of Biosciences ◽

10.4308/hjb.26.1.44 ◽

2019 ◽

Vol 26 (1) ◽

pp. 44

Author(s):

Endang Tri Margawati ◽

Slamet Diah Volkandari ◽

Indriawati Indriawati ◽

Emma M. Svensson

Keyword(s):

Genetic Variation ◽

Bos Taurus ◽

Bos Indicus ◽

Meat Tenderness ◽

Gene Markers ◽

Bos Javanicus ◽

Restriction Fragment Polymorphism ◽

Pcr Rflp ◽

Bali Cattle ◽

Cast Gene

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

Download Full-text

Mineral levels of Bali cattle (Bos javanicus) from different types of land in Bali, Nusa Penida, and Sumbawa Islands (Indonesia)

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201022 ◽

2019 ◽

Vol 20 (10) ◽

Cited By ~ 1

Author(s):

I Nengah Kerta Besung ◽

NI LUH WATINIASIH ◽

GUSTI NGURAH KADE MAHARDIKA ◽

KADEK KARANG AGUSTINA ◽

NI KETUT SUWITI

Keyword(s):

Rice Field ◽

Jugular Vein ◽

Serum Samples ◽

Blood Samples ◽

Land Type ◽

Bos Javanicus ◽

Different Types ◽

Bali Cattle ◽

Flame Method ◽

Mineral Levels

Abstract. Besung INK, Watiniasih NL, Mahardika GNK, Agustina KK, Suwiti NK. 2019. Mineral levels of Bali cattle (Bos javanicus) from four different types of land in different rearing areas in Bali, Nusa Penida, and Sumbawa Islands (Indonesia). Biodiversitas 20: 2931-2936. This study aims to prove that the mineral level of Bali cattle in serum is dependent on the origin and raising habitat. Serum samples were collected from three islands in Indonesia, i.e. Bali, Nusa Penida, and Sumbawa. Samples from Bali were further classified into four different areas (i.e. agroforestry, perennial plantation, horticultural plantation, and rice field). Blood samples were collected in the jugular vein without anticoagulant. The contents of macrominerals (Ca, Mg, Na, K, P) and microminerals (Fe, Cu, Zn, Co, Mn) were measured using the flame method in atomic absorption spectrophotometer. The results showed that the level of phosphor (P) macromineral and all microminerals under study were influenced by the origin of cattle. The macrominerals are statistically varied in different cattle raising environments. The same is also valid for all microminerals, with an exception of Fe. All mineral levels under study were statistically equal in male and female Bali cattle. Phosphor macromineral and all microminerals levels were origin and environment-dependent. Supplementation of deficient minerals in a certain area and land type is recommended to improve the performance of Bali cattle.

Download Full-text

Phylogenetic tree analysis for Bali Cattle based on partial sequence 16S rRNA Mitochondrial DNA

E3S Web of Conferences ◽

10.1051/e3sconf/202233500014 ◽

2022 ◽

Vol 335 ◽

pp. 00014

Author(s):

R. Misrianti ◽

S.H. Wijaya ◽

C. Sumantri ◽

J. Jakaria

Keyword(s):

Phylogenetic Analysis ◽

16S Rrna ◽

Phylogenetic Tree ◽

Bos Taurus ◽

Bos Indicus ◽

Partial Sequence ◽

Bos Grunniens ◽

Bos Javanicus ◽

Mitochondria Dna ◽

Bali Cattle

Mitochondria DNA (mtDNA) as a source of genetic information based on the maternal genome, can provide important information for phylogenetic analysis and evolutionary biology. The objective of this study was to analyze the phylogenetic tree of Bali cattle with seven gene bank references (Bos indicus, Bos taurus, Bos frontalis, and Bos grunniens) based on partial sequence 16S rRNA mitochondria DNA. The Bayesian phylogenetic tree was constructed using BEAST 2.4. and visualization in Figtree 1.4.4 (tree.bio.ed.ac.uk/software/figtree/). The best model of evolution was carried out using jModelTest 2.1.7. The most optimal was the evolutionary models GTR + I + G with p-inv (I) 0,1990 and gamma shape 0.1960. The main result indicated that the Bali cattle were grouped into Bos javanicus. Phylogenetic analysis also successfully classifying Bos javanicus, Bos indicus, Bos taurus, Bos frontalis and Bos grunniens. These results will complete information about Bali cattle and useful for the preservation and conservation strategies of Indonesian animal genetic resources.

Download Full-text