scholarly journals Mineral levels of Bali cattle (Bos javanicus) from different types of land in Bali, Nusa Penida, and Sumbawa Islands (Indonesia)

2019 ◽  
Vol 20 (10) ◽  
Author(s):  
I Nengah Kerta Besung ◽  
NI LUH WATINIASIH ◽  
GUSTI NGURAH KADE MAHARDIKA ◽  
KADEK KARANG AGUSTINA ◽  
NI KETUT SUWITI
Keyword(s):  
Rice Field ◽  
Jugular Vein ◽  
Serum Samples ◽  
Blood Samples ◽  
Land Type ◽  
Bos Javanicus ◽  
Different Types ◽  
Bali Cattle ◽  
Flame Method ◽  
Mineral Levels

Abstract. Besung INK, Watiniasih NL, Mahardika GNK, Agustina KK, Suwiti NK. 2019. Mineral levels of Bali cattle (Bos javanicus) from four different types of land in different rearing areas in Bali, Nusa Penida, and Sumbawa Islands (Indonesia). Biodiversitas 20: 2931-2936. This study aims to prove that the mineral level of Bali cattle in serum is dependent on the origin and raising habitat. Serum samples were collected from three islands in Indonesia, i.e. Bali, Nusa Penida, and Sumbawa. Samples from Bali were further classified into four different areas (i.e. agroforestry, perennial plantation, horticultural plantation, and rice field). Blood samples were collected in the jugular vein without anticoagulant. The contents of macrominerals (Ca, Mg, Na, K, P) and microminerals (Fe, Cu, Zn, Co, Mn) were measured using the flame method in atomic absorption spectrophotometer. The results showed that the level of phosphor (P) macromineral and all microminerals under study were influenced by the origin of cattle. The macrominerals are statistically varied in different cattle raising environments. The same is also valid for all microminerals, with an exception of Fe. All mineral levels under study were statistically equal in male and female Bali cattle. Phosphor macromineral and all microminerals levels were origin and environment-dependent. Supplementation of deficient minerals in a certain area and land type is recommended to improve the performance of Bali cattle.

scholarly journals The Change in External Genital and Estrogen Level of Bali Cattle During Estrus

2020 ◽  
Vol 3 (1) ◽  
pp. 40
Author(s):  
Desak Nyoman Dewi Indira Laksmi ◽  
I Gusti Ngurah Bagus Trilaksana
Keyword(s):  
Jugular Vein ◽  
Reproductive Organs ◽  
Cross Sectional Study ◽  
Vaginal Mucosa ◽  
Cross Sectional ◽  
Blood Samples ◽  
Hormone Levels ◽  
Direct Elisa ◽  
Bali Cattle

The successful application of reproductive technology requires knowledge of estrus cycle and the ability to detect estrus of Bali cattle. Estrus in cattle lasts for 8-30 hours showed by the acceptance of males for copulation. In this phase, estrogen levels reach maximum levels. High levels of estrogen cause signs of estrus, and changes in the reproductive organs. This study aims to determine estrogen levels during estrus and external genital changes during estrus. This research was an observational analytic study with cross-sectional study design. The samples used in this study were Bali cattle during estrus. The samples had healthy status without any signs of disease. Blood samples were taken through the jugular vein were then centrifuged to obtain serum. The obtained serum was examined for estrogen hormone levels. Mechanical measurements of hormone levels checked by using the Direct Elisa, Double Antibody Sandwich. Blood samples were collected from several SIMANTRI in Mengwi District, Badung Regency. The results showed in Bali cattle external genitals change when estrus, namely redness of the vaginal mucosa, discharge from the vagina and swelling of the vulva. While estrogen levels when estrus is 69.80 pg/ ml. Further research needs to be continued to measure the quality of ovulation after estrus.

scholarly journals Correlation between hematological profile and theileriosis in Bali cattle from Muara Bulian, Jambi, Indonesia

2019 ◽  
Vol 12 (9) ◽  
pp. 1358-1361 ◽  
Author(s):  
N. Aziz ◽  
M. Maksudi ◽  
Y. A. Prakoso
Keyword(s):  
Jugular Vein ◽  
Economic Losses ◽  
Parasitic Disease ◽  
Double Staining ◽  
Blood Samples ◽  
Tick Vector ◽  
Blood Tests ◽  
Hematological Profile ◽  
Bali Cattle ◽  
Theileria Spp

Background and Aim: Theileriosis is a parasitic disease caused by the hemoprotozoan Theileria spp. The main transmission route of this disease is through tick vector bite. Theileriosis causes economic losses in livestock such as Bali cattle. This study aims to analyze the prevalence of theileriosis and its correlation with the hematological profile of Bali cattle from the subdistrict of Muara Bulian, Batang Hari, Jambi, Indonesia. Materials and Methods: Ninety-four blood samples were collected through jugular vein of Bali cattle. The presence of Theileria spp. was determined using blood smear. Routine blood tests and double-staining immunohistochemistry against CD4+ and CD8+ lymphocytes were conducted on all blood samples. Results: A total of 34.04% of the samples were infected by Theileria spp. Theileriosis affected only hemoglobin level (p<0.05); it did not affect the other parameters of the hematological profile (p>0.05). However, it also decreased CD4+ and CD4+/CD8+ ratio (p<0.05), besides increasing CD8+ (p<0.05). Conclusion: Theileriosis does not change the hematological profile of Bali cattle except for the hemoglobin levels. Moreover, it promotes T-cell depletion.

scholarly journals Diseases Characteristic of Pre-Weaning Bali Calf (Bos Javanicus) in Central Lombok, Indonesia

2019 ◽  
Vol 3 (1) ◽  
pp. 236
Author(s):  
Adji Santoso Dradjat ◽  
Muhamad Imran ◽  
T.S. Panjaitan ◽  
Dahlanuddin Dahlanuddin
Keyword(s):  
High Temperature ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Rainy Season ◽  
Liver Fluke ◽  
Macrocytic Anemia ◽  
Blood Samples ◽  
Bos Javanicus ◽  
Internal Parasites ◽  
Bali Cattle

The aim of the study was to evaluate Bali cattle pre-weaning calf death in Central Lombok. Sick calves were examined their condition, followed by physiology examination. Then feces and blood samples were collected and examined for internal parasites and hematology. The results showed that the newborn calf was 57,2% (61), while calves sick was 16.5% (101) consisted of 7,2% (57) death calves and 9,3% still alive. Calves examination showed that 21, 05 % (12) was under weak conditions, 38.5% (22) with high temperature, 73, 5% (42) calves were found diarrhea. This diarrhea calves consisted of 50,8% (29) diarrhea, 3, 5% (2) diarrhea with blood stain, and 19, 2% (11) diarrhea with high temperature. The results of coproscopy showed that 14.03% (8) calves diarrhea were infected with parasites, such as protozoa (Eimeria sp), liver fluke (Fasciola sp) and gastro intestine worm (Trichuris sp, Trichostrongilus sp, and Bunostonum sp). Following evaluation of hematocrit, it was found that 33.3% (19) calves higher while 3.50% (2) lower. Following examination of Red Blood Cell, it was found that 98.2% (56) calves showed anemia hypochromic, 28.07% (16) showed anemia microcytic, 15,7% (9) macrocytic anemia, 5.2% (3) anemia hypochromic, 3.5% (2 calves) showed erythropenia. Leucocytes examinations showed that 35.08% (20 calves) showed by lymphocytosis, 7.01% (4 calves) leucocytosis, 1.75% (1 calf) leucopenia and 1.75% (1 calf) lymphocytopenia. It can be concluded that from the ill calves in the middle of the rainy season showed diarrhea with protozoa, liver fluke, and worms infestations. Hematology examination the sick calves indicated that there were anemia, lymphocytosis, dehydration, low content of blood corpuscular. Finally, the sick calves may be infected with parasites, protozoa’s, bacteria’s and viruses.

scholarly journals JUMLAH ERITROSIT DAN NILAI HEMATOKRIT SAPI ACEH DAN SAPI BALI DI KECAMATAN LEUMBAH SEULAWAH KABUPATEN ACEH BESAR (Total Erythrocytes Count and Haematocrit Value of Aceh and Bali Cattle in Leumbah Seulawah, Aceh Besar)

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Mulyadi Adam ◽  
Triva Murtina Lubis ◽  
Baradillah Abdyad ◽  
Nuzul Asmilia ◽  
Muttaqien Muttaqien ◽  
...  
Keyword(s):  
Jugular Vein ◽  
Blood Samples ◽  
Male And Female ◽  
Significant Difference ◽  
Bali Cattle

The aim of this research was to find out total erythrocytes count and hematocrit value of aceh and bali cattle in Leumbah Seulawah, Aceh Besar. Ten aceh cattle (5 males and 5 females with the age of 2.5-3.0 years) and ten bali cattle (5 males and 5 females aged 2.5-3.0 years) from Leumbah Seulawah, Aceh Besar were used in this research. Blood samples approximately 3 cc were collected by venoject through jugular vein. Erythrocyte counts were performed using a hemocytometer and hematocrit value were performed by microhematocrit method. The average of erythrocyte counts of male and female aceh cattle were 7.75 ±1.86 and 7.34±0.73 (106/mm3) respectively, while male and female bali cattle were 5.49±0.88 and 4.89±0.53 (106/mm3) respectively. Hematocrit value of male and female aceh cattle were 32.8±3.70 dan 32.6±207% respectively, while male and female bali cattle were 27.4±3.00 and 25.8±1.30% respectively. In conclusion, erythrocytes count and hematocrit value of aceh cattle show significant difference (P<0.01) from bali cattle.Key words: erythrocytes, hematocrit, aceh cattle, bali cattle

scholarly journals Biochemical and haematological profiles of black Bengal goat in Mymensingh sadar of Bangladesh

2022 ◽  
Vol 8 (3) ◽  
pp. 329-337
Author(s):  
Kanika Samaddar ◽  
Md Mizanur Rahman ◽  
Ziaul Haque
Keyword(s):  
Total Protein ◽  
Significant Variation ◽  
Biochemical Analysis ◽  
Jugular Vein ◽  
Serum Samples ◽  
Blood Samples ◽  
Variation Of Parameters ◽  
Leukocyte Counts ◽  
Biochemical Profiles ◽  
Black Bengal Goat

The aim of the present study was to know the normal haemato- biochemical profiles of black Bengal goats in Bangladesh. Blood samples were collected from Jugular vein of 20 Black Bengal goats which were divided into 5 groups (4 goats in each group) on the basis of age, sex, nutritional and feeding status. The whole blood was analyzed for hematology, plasma and serum samples for biochemical analysis. The results of the present study demonstrate the normal haemato-biochemical values of the Black Bengal goat breeds with their age specificity. Total RBC (15.15 ± 1.29 million/cumm), haemoglobin (7.04 ± 1.02 g/dl), total WBC (11.64 ± 1.12), differential leukocyte counts of neutrophils (70.89 ± 2.06%), lymphocyte (23.79 ± 1.69%), eosinophil 2.1%), basophil (2.3%), MCV (35.04 ± 0.47) and PCV (26.98 ± 1.49%). This study also reveals the normal bio-logical profile counts as total protein (69.47±2.31 g/L), Albumin (42.47 ± 2.31g/L), Globulin (27.32g/L), Phosphorus (3.46 ± 1.19), blood Urea (22.97 ± 0.58), Creatinine (0.645mg/dl), ALT (20.32U/L), ALP (91.59 U/L), GGT (37.46 U/L), IgG (18.91 ± 1.14 g/L), IgM ( 2.87 ± 0.18g/L), gA (.031 ± 0.12g/L) and IL-6 (1.69±0.15g/ml). The study found higher number of RBC (million/cumm), WBC (Thousands/cumm), and PCV% in the goats of age 3 years and above (18.02 ± 1.27, 14.72 ± 1.19, and 28 ± 3.2), respectively. Besides, Neutrophils% and total protein was found higher (76.289 ± 14.5%, 77.52 ± 8.43 g/L), respectively in the Black Bengal goats of 6 months to 1 year age. Present results stated some significant variation of parameters between goats of various ages; on the other hand, some shows no significant variation at all. Res. Agric., Livest. Fish.8(3): 329-337, December 2021

Association of Retained Fetal Membranes with Haematological Profile in Crossbred Cows

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
K. Rokde ◽  
S. Kumar ◽  
A. Bhardwaz ◽  
S. S. Mahour ◽  
S. P. Nema ◽  
...  
Keyword(s):  
Eosinophil Count ◽  
Jugular Vein ◽  
Dairy Farm ◽  
Fetal Membranes ◽  
Monocyte Count ◽  
Blood Samples ◽  
Mean Values ◽  
Crossbred Cows ◽  
Haematological Profile ◽  
The Mean

This study was carried out on clinical cases of retained fetal membranes in crossbred cows presented at College Clinics and College dairy farm and from Villages in and around Mhow. The blood samples were collected from jugular vein just before 12 hr. postpartum and on 7th day postpartum. Haematological profile revealed that the mean values of haemoglobin, neutrophil and monocyte count after 12 hrs and 7th day postpartum were significantly lower and lymphocyte count was significantly higher in RFM cows (n=18) than normally calved cows (n=6). The differences in mean TLC, eosinophil and basophil counts were non-significant at 12 hrs postpartum, however on 7th day postpartum the TLC and eosinophil count were significantly higher and basophil count was non-significantly different in RFM cows than the normally calved cows.

Serum Endocrine Profile in Relation to Estrus Synchronization in Surti Does

2018 ◽  
Vol 13 (03) ◽  
Author(s):  
M. M. Chaudhary ◽  
C. T. Khasatiya ◽  
S. B. Patel ◽  
S. S. Chaudhary ◽  
V. B. Atara ◽  
...  
Keyword(s):  
Jugular Vein ◽  
Estrus Synchronization ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Group Iii ◽  
Group I ◽  
Endocrine Profile ◽  
Group Ii ◽  
And Control

The serum progesterone and estradiol profiles during synchronization of estrus by buck effect and PGF2α treatments were monitored in Surti does. Total eighteen non-pregnant does selected were evenly divided into 3 groups, 6 does in each group. The does of Group I were teased with a sexuallyactive- apronized buck; and those of Group II were treated with PGF2α, i.e., Inj. Lutalyse® @ 7.5 mg/doe IM twice 11 days apart, while the Group III served as untreated control. Blood samples were collected from all the animals on day 0 (before 1st PGF2α injection), 3rd day (during treatment), 11th day (before 2nd PGF2α injection), 14th day (after treatment) and 40th day (post-service) by jugular vein puncture. The serum separated was stored at -20°C till further analysis. In all the three groups, 83.33% does, conceived at first service in the sampling cycle. The overall mean serum progesterone concentration of Group I does (5.82±0.72 ng/ml) was significantly higher (p less than 0.01) as compared to Group II (2.93±0.38 ng/ml) and III (2.88±0.30 ng/ml). Similarly, the overall mean serum progesterone concentration of Surti does on day 0 (2.65±0.46 ng/ml), 3rd (2.56±0.80 ng/ml), 11th (4.45±0.84 ng/ml) and 14th (3.40±0.63 ng/ml) did not differ significantly, but the overall mean level at day 40 (6.31±0.45 ng/ml) was significantly (p less than 0.01) higher, because most of animals became pregnant at that time. The overall mean serum oestradiol-17β levels of Group I (24.40±2.98 pg/ ml) was significantly higher (p less than 0.01) than in Group II (15.77±1.77 pg/ml) and III (12.21±1.45 pg/ ml). On the other hand, the overall mean serum oestradiol-17β levels of Surti does on day 0 (12.89±1.21 pg/ml), 3rd (15.84±1.74 pg/ml), 11th (14.81±1.96 pg/ml), 14th (22.15±2.97 pg/ml) and 40th (21.64±5.16 pg/ml) did not differ significantly (p>0.05) and the slightly higher overall mean level found at 40th day might be the influence of the non-pregnant does at first service in the cumulative animals. The hormonal profile reflected the initiation of cyclicity and establishment of pregnancy in treated and control animals.

scholarly journals PRRSV detection by qPCR in processing fluids and serum samples collected in a positive stable breeding herd following mass vaccination of sows with a modified live vaccine

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
A. Lebret ◽  
P. Berton ◽  
V. Normand ◽  
I. Messager ◽  
N. Robert ◽  
...  
Keyword(s):  
The United States ◽  
Live Vaccine ◽  
Mass Vaccination ◽  
Respiratory Syndrome Virus ◽  
Serum Samples ◽  
Blood Samples ◽  
Stability Monitoring ◽  
Breeding Herd ◽  
Modified Live Vaccine ◽  
Processing Fluid

AbstractIn the last two decades, in France, Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) stabilization protocols have been implemented using mass vaccination with a modified live vaccine (MLV), herd closure and biosecurity measures. Efficient surveillance for PRRSV is essential for generating evidence of absence of viral replication and transmission in pigs. The use of processing fluid (PF) was first described in 2018 in the United States and was demonstrated to provide a higher herd-level sensitivity compared with blood samples (BS) for PRRSV monitoring. In the meantime, data on vertical transmission of MLV viruses are rare even as it is a major concern. Therefore, veterinarians usually wait for several weeks after a sow mass vaccination before starting a stability monitoring. This clinical study was conducted in a PRRSV-stable commercial 1000-sow breed-to-wean farm. This farm suffered from a PRRS outbreak in January 2018. After implementing a stabilisation protocol, this farm was controlled as stable for more than 9 months before the beginning of the study. PF and BS at weaning were collected in four consecutive batches born after a booster sow mass MLV vaccination. We failed to detect PRRSV by qPCR on PF and BS collected in a positive-stable breeding herd after vaccination with ReproCyc® PRRS EU (Boehringer Ingelheim, Ingelheim, Germany).

scholarly journals Surrogate Indexes of Insulin Resistance in Dairy Goats: Transitional Variation in Subclinical Hyperketonemia

2021 ◽  
Vol 8 (6) ◽  
pp. 102
Author(s):  
Siqi Liu ◽  
Yezi Kong ◽  
Jing Wen ◽  
Yan Huang ◽  
Yaoquan Liu ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Transition Period ◽  
Jugular Vein ◽  
Metabolic Parameters ◽  
Model Assessment ◽  
Early Lactation ◽  
Homeostasis Model Assessment ◽  
Dairy Goats ◽  
Blood Samples ◽  
Normal Glucose

Background: Dairy goats are highly susceptible to subclinical hyperketonemia (SCHK) during the transition period. This study aimed to compare the variation in metabolic parameters and surrogate indexes of insulin resistance (sIR) between goats with SCHK and clinically healthy (HEAL) goats during the transition period. Methods: Twenty Guanzhong dairy goats were assorted to HEAL (n = 10) and SCHK (n = 10) groups according to the blood β-hydroxybutyrate (BHBA) concentrations. The blood samples were taken from the jugular vein of each goat at −3, −2, −1, 0 (partum), +1, +2, and +3 weeks relative to kidding to analyses GLU and INS. The sIR was calculated from blood metabolic parameters. Results: Compared with the HEAL goats, the insulin concentrations were significantly higher in SCHK goats during the first three weeks postpartum. The QUICKI, revised QUICKI (RQUICKI), and RQUICKIBHBA were significantly lower in goats with SCHK at 1 week postpartum, while the homeostasis model assessment-IR (HOMA-IR) was significantly higher. Conclusion: Goats with SCHK made more efforts through elevated insulin levels at early lactation than HEAL goats, thereby maintaining the normal glucose concentrations.

Search for Melanoma Markers in Plasma and Serum Samples

2005 ◽  
Vol 11 (3) ◽  
pp. 353-360 ◽  
Author(s):  
Roberta Seraglia ◽  
Susanna Vogliardi ◽  
Graziella Allegri ◽  
Stefano Comai ◽  
Mario Lise ◽  
...  
Keyword(s):  
High Frequency ◽  
Healthy Subjects ◽  
Ionic Species ◽  
Low Molecular Weight ◽  
Ionization Mass ◽  
Plasma Samples ◽  
Serum Samples ◽  
Blood Samples ◽  
Melanoma Patients ◽  
Diagnostic Ions

Fourteen blood samples from patients with melanomas and 11 blood samples from healthy subjects were analyzed by matrix-assisted laser desorption/ionization mass spectrometry. The study focussed on species of low molecular weight, in the 800–5000 Da range, present in plasma and sera. While for healthy subjects plasma samples lead to the production of a higher number of ionic species, for melanoma patients a high number of diagnostic ions, present with high frequency and with quite high relative abundance, are present, in particular, in serum samples and, to a lesser extent, also in plasma. Since plasma samples are obtained more easily in comparison to sera, it is possible to suggest that plasma can also be used for these studies.

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