Gene Expression and Protein Production of Leukemia Inhibitory Factor in Human Dental Follicle Cells

2013 ◽  
Vol 11 (3) ◽  
pp. 156-162
Author(s):  
Yoko Fujimoto ◽  
Naomi Ogura ◽  
Haruna Aonuma ◽  
Kosuke Takahashi ◽  
Ko Ito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Leukemia Inhibitory Factor ◽  
Protein Production ◽  
Inhibitory Factor ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

Regulation of Osteoprotegerin Gene Expression in Dental Follicle Cells

2003 ◽  
Vol 82 (4) ◽  
pp. 298-302 ◽  
Author(s):  
G.E. Wise ◽  
Y. Ren ◽  
S. Yao
Keyword(s):  
Gene Expression ◽  
Steady State ◽  
Protein Kinase ◽  
Follicle Cells ◽  
Protein Kinase Activity ◽  
Mrna Levels ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Parathyroid Hormone Related Protein ◽  
Mandibular Molar

Colony-stimulating factor-one (CSF-1) and parathyroid-hormone-related protein (PTHrP) down-regulate osteoprotegerin (OPG) gene expression in the dental follicle of the rat first mandibular molar. To examine this regulation at the signal transduction level, we treated cultured dental follicle cells with either phorbolmyristate acetate (PMA) or dibutyryl cyclic AMP (dbcAMP) to activate either protein kinase C (PKC) or protein kinase A (PKA). Our results demonstrate that PMA up-regulates OPG gene expression and down-regulates the expression of CSF-1 and the PTHrP receptor (PTHrP-R). Conversely, dbcAMP down-regulates OPG expression and up-regulates CSF-1 and PTHrP-R expression. Immunostaining shows that PMA also increases the steady-state levels of protein. Thus, treatment with agents that affect protein kinase activity also enhance the steady-state mRNA and protein levels of OPG, as well as decreasing the mRNA levels of CSF-1 and PTHrP-R. The PKC-α isoform may be critical in OPG regulation because PKC-α gene expression is enhanced by PMA and reduced by either CSF-1 or PTHrP.

PDGF BB and bFGF stimulate DNA synthesis and upregulate CSF-1 and MCP-1 gene expression in dental follicle cells

2003 ◽  
Vol 48 (6) ◽  
pp. 459-465 ◽  
Author(s):  
S Bsoul ◽  
G Terezhalmy ◽  
H Abboud ◽  
K Woodruff ◽  
S.L Abboud
Keyword(s):  
Gene Expression ◽  
Dna Synthesis ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

The Differentiation and Gene Expression Profile of Human Dental Follicle Cells

2010 ◽  
Vol 19 (5) ◽  
pp. 707-717 ◽  
Author(s):  
Michael Saugspier ◽  
Oliver Felthaus ◽  
Sandra Viale-Bouroncle ◽  
Oliver Driemel ◽  
Torsten E. Reichert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

scholarly journals The expressions of tooth eruption relevant genes are different in incisors and molars dental follicle cells in rat: an in vitro study

2019 ◽  
Author(s):  
Mengting He ◽  
Xiaomeng Dong ◽  
Peiqi Wang ◽  
Zichao Xiang ◽  
Jiangyue Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expression Patterns ◽  
In Vitro Study ◽  
Tooth Eruption ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Real Time Qpcr

Abstract Background The incisors and molars showed different patterns of tooth eruption in rodents and the dental follicle cells play key roles in tooth eruption. Little is known about the differences in incisors and molars dental follicle cells during tooth eruption in rodents. The purpose of this study was to investigate the differences between incisor dental follicle cells and molar dental follicle cells during tooth eruption in rat.Methods Incisor dental follicle cells and molar dental follicle cells were obtained as previously described. Immunofluorescence was used to identify the cells. Gene expression was measured by real-time qPCR and western blot.Results Compared with molar dental follicle cells, the incisor dental follicle cells showed higher expression of OPG, BMP-2 and BMP-3. The molar dental follicle cells showed higher expression of MCP-1 and RANKL.Conclusions The expression patterns of genes related to tooth eruption were different in incisors and molars dental follicle cells in rat.

Gene expression of Semaphorin 7A in human dental follicle cells

2014 ◽  
Vol 72 (9) ◽  
pp. e194-e195
Author(s):  
K. Takahashi ◽  
N. Ogura ◽  
R. Kato ◽  
R. Tomoki ◽  
T. Eda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

scholarly journals Capacity of Human Dental Follicle Cells to Differentiate into Neural CellsIn Vitro

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Shingo Kanao ◽  
Naomi Ogura ◽  
Kosuke Takahashi ◽  
Ko Ito ◽  
Masaaki Suemitsu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Neuronal Differentiation ◽  
Tooth Germ ◽  
Follicle Cells ◽  
Neural Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Neural Markers ◽  
Sphere Formation

The dental follicle is an ectomesenchymal tissue surrounding the developing tooth germ. Human dental follicle cells (hDFCs) have the capacity to commit to differentiation into multiple cell types. Here we investigated the capacity of hDFCs to differentiate into neural cells and the efficiency of a two-step strategy involving floating neurosphere-like bodies for neural differentiation. Undifferentiated hDFCs showed a spindle-like morphology and were positive for neural markers such as nestin,β-III-tubulin, and S100β. The cellular morphology of several cells was neuronal-like including branched dendrite-like processes and neurites. Next, hDFCs were used for neurosphere formation in serum-free medium containing basic fibroblast growth factor, epidermal growth factor, and B27 supplement. The number of cells with neuronal-like morphology and that were strongly positive for neural markers increased with sphere formation. Gene expression of neural markers also increased in hDFCs with sphere formation. Next, gene expression of neural markers was examined in hDFCs during neuronal differentiation after sphere formation. Expression ofMusashi-1andMusashi-2,MAP2,GFAP,MBP, andSOX10was upregulated in hDFCs undergoing neuronal differentiation via neurospheres, whereas expression ofnestinand β-III-tubulinwas downregulated. In conclusion, hDFCs may be another optimal source of neural/glial cells for cell-based therapies to treat neurological diseases.

Gene expression profiles of dental follicle cells before and after osteogenic differentiation in vitro

2009 ◽  
Vol 13 (4) ◽  
pp. 383-391 ◽  
Author(s):  
Christian Morsczeck ◽  
Gottfried Schmalz ◽  
Torsten Eugen Reichert ◽  
Florian Völlner ◽  
Michael Saugspier ◽  
...  
Keyword(s):  
Gene Expression ◽  
Osteogenic Differentiation ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Before And After
Sign in / Sign up

Export Citation Format

Share Document