Molecular characterization of the flavonoid biosynthetic pathway and flower color modification of Nierembergia sp

2006 ◽  
Vol 23 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Yukiko Ueyama ◽  
Yukihisa Katsumoto ◽  
Yuko Fukui ◽  
Masako Fukuchi-Mizutani ◽  
Hideo Ohkawa ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Biosynthetic Pathway ◽  
Flower Color ◽  
Flavonoid Biosynthetic Pathway ◽  
Color Modification

Flower Color Modification by Engineering of the Flavonoid Biosynthetic Pathway: Practical Perspectives

2010 ◽  
Vol 74 (9) ◽  
pp. 1760-1769 ◽  
Author(s):  
Yoshikazu TANAKA ◽  
Filippa BRUGLIERA ◽  
Gianna KALC ◽  
Mick SENIOR ◽  
Barry DYSON ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Flower Color ◽  
Flavonoid Biosynthetic Pathway ◽  
Color Modification

Bioinfomatics Analysis of the Anthocyanidin synthase Gene in Tree Peony

2014 ◽  
Vol 1010-1012 ◽  
pp. 1181-1184
Author(s):  
Yan Zhao Zhang ◽  
Yan Wei Cheng ◽  
Hui Yuan Ya ◽  
Chao Yun ◽  
Jian Ming Han ◽  
...  
Keyword(s):  
Plant Species ◽  
Structure Prediction ◽  
Biosynthetic Pathway ◽  
Resistance Breeding ◽  
Flower Color ◽  
Anthocyanin Synthesis ◽  
Tree Peony ◽  
Reading Frame ◽  
Transcriptome Database ◽  
Color Modification

Anthocyanin mainly responsible for flowers color in many plant species, it also accumulated in response to lots of environmental stress to reduce the damage to plant cell. Anthocyanin synthesis (ANS) protein is an important synthetase participated in anthocyanin biosynthetic pathway. In this study, we isolated the PsANS gene from transcriptome database built by our previous study. The PsANS gene contain an 1050bp open reading frame encoding 349 amino acid, phylogenetic analysis revealed that PsANS was segrated into a group with ANS from others plant species. Secondary and thri-dimension structure prediction also revealed that it may have similar function with ANS in others plant species. The identified PsANS gene would be helpful for further research in flower color modification and resistance breeding.

Molecular characterization of EgMYB1, a putative transcriptional repressor of the lignin biosynthetic pathway

Plant Science ◽  
2007 ◽  
Vol 173 (5) ◽  
pp. 542-549 ◽  
Author(s):  
Sylvain Legay ◽  
Eric Lacombe ◽  
Monica Goicoechea ◽  
Christian Brière ◽  
Armand Séguin ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Biosynthetic Pathway ◽  
Transcriptional Repressor ◽  
Lignin Biosynthetic Pathway

scholarly journals Changes at a Critical Branchpoint in the Anthocyanin Biosynthetic Pathway Underlie the Blue to Orange Flower Color Transition in Lysimachia arvensis

2021 ◽  
Vol 12 ◽  
Author(s):  
Mercedes Sánchez-Cabrera ◽  
Francisco Javier Jiménez-López ◽  
Eduardo Narbona ◽  
Montserrat Arista ◽  
Pedro L. Ortiz ◽  
...  
Keyword(s):  
Differential Expression ◽  
Biosynthetic Pathway ◽  
Biochemical Analysis ◽  
Flower Color ◽  
Careful Examination ◽  
Structural Genes ◽  
Significant Differential Expression ◽  
Flavonoid Biosynthetic Pathway ◽  
Flower Color Polymorphism ◽  
Survival And Reproduction

Anthocyanins are the primary pigments contributing to the variety of flower colors among angiosperms and are considered essential for survival and reproduction. Anthocyanins are members of the flavonoids, a broader class of secondary metabolites, of which there are numerous structural genes and regulators thereof. In western European populations of Lysimachia arvensis, there are blue- and orange-petaled individuals. The proportion of blue-flowered plants increases with temperature and daylength yet decreases with precipitation. Here, we performed a transcriptome analysis to characterize the coding sequences of a large group of flavonoid biosynthetic genes, examine their expression and compare our results to flavonoid biochemical analysis for blue and orange petals. Among a set of 140 structural and regulatory genes broadly representing the flavonoid biosynthetic pathway, we found 39 genes with significant differential expression including some that have previously been reported to be involved in similar flower color transitions. In particular, F3′5′H and DFR, two genes at a critical branchpoint in the ABP for determining flower color, showed differential expression. The expression results were complemented by careful examination of the SNPs that differentiate the two color types for these two critical genes. The decreased expression of F3′5′H in orange petals and differential expression of two distinct copies of DFR, which also exhibit amino acid changes in the color-determining substrate specificity region, strongly correlate with the blue to orange transition. Our biochemical analysis was consistent with the transcriptome data indicating that the shift from blue to orange petals is caused by a change from primarily malvidin to largely pelargonidin forms of anthocyanins. Overall, we have identified several flavonoid biosynthetic pathway loci likely involved in the shift in flower color in L. arvensis and even more loci that may represent the complex network of genetic and physiological consequences of this flower color polymorphism.

scholarly journals Transcriptome-Wide Analysis Reveals Key DEGs in Flower Color Regulation of Hosta plantaginea (Lam.) Aschers

Genes ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 31
Author(s):  
Jingying Zhang ◽  
Changhai Sui ◽  
Yanli Wang ◽  
Shuying Liu ◽  
Huimin Liu ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Biosynthetic Pathway ◽  
Flower Color ◽  
White Flower ◽  
Flowering Stage ◽  
Purple Flower ◽  
Late Flowering ◽  
Qrt Pcr ◽  
Flavonoid Biosynthetic Pathway ◽  
Color Regulation

Background: Hosta plantaginea (Lam.) Aschers (HPA), a species in the family Liliaceae, is an important landscaping plant and herbaceous ornamental flower. However, because the flower has only two colors, white and purple, color matching applications are extremely limited. To date, the mechanism underlying flower color regulation remains unclear. Methods: In this study, the transcriptomes of three cultivars—H. plantaginea (HP, white flower), H. Cathayana (HC, purple flower), and H. plantaginea ‘Summer Fragrance’ (HS, purple flower)—at three flowering stages (bud stage, initial stage, and late flowering stage) were sequenced with the Illumina HiSeq 2000 (San Diego, CA, USA). The RNA-Seq results were validated by qRT-PCR of eight differentially expressed genes (DEGs). Then, we further analyzed the relationship between anthocyanidin synthase (ANS), chalcone synthase (CHS), and P450 and the flower color regulation by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Eukaryotic Orthologous Groups (KOG) network and pathway enrichment analyses. The overexpression of CHS and ANS in transgenic tobacco petals was verified using qRT-PCR, and the petal colors associated with the overexpression lines were confirmed using absorbance values. Results: Over 434,349 transcripts were isolated, and 302,832 unigenes were identified. Additionally, through transcriptome comparisons, 2098, 722, and 606 DEGs between the different stages were found for HP, HC, and HS, respectively. Furthermore, GO and KEGG pathway analyses showed that 84 color-related DEGs were enriched in 22 pathways. In particular, the flavonoid biosynthetic pathway, regulated by CHS, ANS, and the cytochrome P450-type monooxygenase gene, was upregulated in both purple flower varieties in the late flowering stage. In contrast, this gene was hardly expressed in the white flower variety, which was verified in the CHS and ANS overexpression transgenic tobacco petals. Conclusions: The results suggest that CHS, ANS, and the cytochrome P450s-regulated flavonoid biosynthetic pathway might play key roles in the regulation of flower color in HPA. These insights into the mechanism of flower color regulation could be used to guide artificial breeding of polychrome varieties of ornamental flowers.

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