Single Factor Effect of Natural Deep Eutectic Solvent Citric Acid-Glucose Based Microwave-Assisted Extraction on Total Polyphenols Content from Mitragyna speciosa Korth. Havil Leaves

Application of natural deep eutectic solvent (NADES) citric acid-glucose-based microwave-assisted extraction (MAE) method for total polyphenol content (TPC) enriches the use of Eleutherine bulbosa (Mill.) bulb more widely and commercially. Therefore, this study aims to optimize NADES citric acid-glucose-based MAE on the TPC from E. bulbosa bulbs using response surface methodology (RSM). In the present study, the dried sample of E. bulbosa bulb was extracted using the NADES based MAE. Determination of TPC was conducted using Folin-Ciocalteau reagent and standard gallic acid, then measured using a UV-Vis spectrophotometer at 761 nm. Extraction condition optimization of the NADES based MAE method was performed using RSM with Box Behnken Design (four-factors-three-levels and 29 runs) on Design Expert v12. Based on the results study, the optimum condition extraction on the TPC enrichment was obtained at the NADES ratio (citric acid: glucose) 1:1 g/g; solid-liquid ratio 1:8 g/mL; extraction time of 15 min; and 270 Watt microwave power. The confirmation test and scale-up (50 g samples) were obtained with a 61.63 ± 2.23 mg GA/g sample.

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Ekstraksi Polifenol Total dari Herba Suruhan (Peperomia pellucida [L.] KUNTH) Menggunakan Metode Citric Acid-Glucose Based Microwave Assisted Extraction

Proceeding of Mulawarman Pharmaceuticals Conferences ◽

10.25026/mpc.v10i1.363 ◽

2019 ◽

Vol 10 ◽

pp. 76-80

Author(s):

Duwi Meiliyani ◽

Sabaniah ◽

Islamudin Ahmad

Keyword(s):

Citric Acid ◽

Extraction Method ◽

Microwave Assisted Extraction ◽

Green Solvent ◽

Liquid Ratio ◽

Extraction Condition ◽

Total Polyphenols ◽

Microwave Assisted ◽

Assisted Extraction ◽

Solid Liquid

This study aims to determine the effect of citric acid-glucose based microwave assited extraction (MAE) method on total polyphenols content from (Peperomia pellucida [L.] Kunth) herbs. Dried samples were extracted using green solvent glucose citric acid with a ratio of 1: 1 g/g, 3: 1 g/g, and 5: 1 g/g, respectively, 30% microwave power for 5 minutes, and 1:5 g /mL solid-liquid ratio. Then the obtained extract solution was performed determination total polyphenols content using Folin-Ciocalteu reagent, and the absorbance was measured using a UV-Vis spectrophotometer at 791 nm. Based on the results, obtained total polyphenols content according to the extraction condition namely 214.405 mg GAE/g sample (3: 1 g /g solvent), 84.833 mg GAE /g sample (1: 1 g /g solvent) and 53,9451 mg GAE/g sample (5: 1 g /g solvent), respectively. This study is a preliminary data about the use of citric acid-glucose based microwave assisted extraction method on total polyphenols content from this plant quickly, easily and efficiently.

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Development of a colorless Centella asiatica (L.) Urb. extract using a natural deep eutectic solvent (NADES) and microwave-assisted extraction (MAE) optimized by response surface methodology

RSC Advances ◽

10.1039/d0ra09934a ◽

2021 ◽

Vol 11 (15) ◽

pp. 8741-8750

Author(s):

Suppalak Phaisan ◽

Fonthip Makkliang ◽

Waraporn Putalun ◽

Seiichi Sakamoto ◽

Gorawit Yusakul

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Deep Eutectic Solvent ◽

Centella Asiatica ◽

Microwave Assisted Extraction ◽

Green Process ◽

Microwave Assisted ◽

Assisted Extraction ◽

Natural Deep Eutectic Solvent

This study outlines a green process for Centella asiatica (L.) Urb. (CA) extraction.

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Pengayaan Polifenol Total dari Daun Kadamba Menggunakan Metode Ekstraksi Berbantu Mikrowave Berbasis Pelarut Hijau

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2020.v6.i2.15035 ◽

2020 ◽

Vol 6 (2) ◽

Author(s):

Islamudin Ahmad ◽

Andi Yusniah ◽

Yuspian Nur ◽

Wisnu Cahyo Prabowo ◽

Herman

Keyword(s):

Malic Acid ◽

Deep Eutectic Solvent ◽

Green Solvent ◽

Optimum Conditions ◽

Total Polyphenols ◽

Box Behnken Design ◽

Mitragyna Speciosa ◽

Assisted Extraction ◽

Natural Deep Eutectic Solvent ◽

Optimum Extraction

The present study aims to optimize the natural deep eutectic solvent (NADES) as a green solvent-based microwave-assisted extraction (MAE) of total polyphenols content (TPC) from Mitragyna speciosa Korth Havil leaves using response surface methodology (RSM). Preparation of natural deep eutectic solvent (NADES) as a green solvent was performed by melting the two-component of malic acid and glucose using a magnetic stirrer. The leaves sample was extracted using the NADES-MAE method with various extraction conditions with four factors and three levels (Box Behnken Design) and optimized using RSM with licensed software of Design Expert V12. TPC was determined using a Folin-Ciocalteau reagent and absorbance was measured on a UV-VIS spectrophotometer at 770 nm and gallic acid as a standard. This study was obtained the optimum extraction conditions include: NADES ratio (malic acid: glucose) of 1:2 g/g, 50% microwave power, a solvent-sampel ratio of 12:1 mL/g, and extraction time for 7 minutes. The optimum conditions were obtained according to the equation formula: TPC = 165.17 – 33.97X1 + 38.36X2 – 6.08X3 + 16.12X4 – 62.77X1X2 + 79.61X12X2, where a R2 value = 0.7102 with a TPC prediction of 402.087±43.81 mg GAE/g sample. These conditions can be used to extract TPC from this plant efficiently, quickly, easily, and environmentally friendly.

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Citric acid-based deep eutectic solvent for the anthocyanin recovery from Hibiscus sabdariffa through microwave-assisted extraction

Biomass Conversion and Biorefinery ◽

10.1007/s13399-020-00606-3 ◽

2020 ◽

Cited By ~ 7

Author(s):

Ebru Kurtulbaş ◽

Ayşe Gizem Pekel ◽

Mehmet Bilgin ◽

Dimitris P. Makris ◽

Selin Şahin

Keyword(s):

Citric Acid ◽

Deep Eutectic Solvent ◽

Hibiscus Sabdariffa ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction

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Deep Eutectic Solvent-Based Microwave-Assisted Extraction of Baicalin from Scutellaria baicalensis Georgi

Journal of Chemistry ◽

10.1155/2018/9579872 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 6

Author(s):

Hui Wang ◽

Xiaodi Ma ◽

Qibin Cheng ◽

Xiaoli Xi ◽

Liwei Zhang

Keyword(s):

Maximum Yield ◽

Deep Eutectic Solvent ◽

Extraction Yield ◽

Scutellaria Baicalensis ◽

Molar Ratio ◽

Extraction Temperature ◽

Microwave Assisted Extraction ◽

Scutellaria Baicalensis Georgi ◽

Microwave Assisted ◽

Assisted Extraction

Deep eutectic solvents (DESs) have attracted significant attention as green media for the extraction and separation of natural compounds from Chinese medicine. In this study, a hydrophobic DESs-based microwave-assisted extraction (MAE) was successfully used to efficiently extract baicalin from Scutellaria baicalensis Georgi. Firstly, DecA: N4444-Cl (DES-1 , molar ratio 1 : 2) was screened and selected as the most appropriate DES by comparing the extraction yield in different hydrophobic DESs. Based on the extraction yield of baicalin, response surface methodology (RSM) was employed to model and optimize the parameters (extraction temperature, liquid-solid ratio, and extraction time). Furthermore, the maximum yield of 106.96 mg·g−1 was achieved under optimum conditions in DES-containing aqueous solutions (33 vol% water content), which reached a similar level that was conducted using the pharmacopoeia procedure (104.94 mg·g−1). These results indicated that the proposed method is an excellent alternative for the extraction of baicalin.

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Ekstraksi Polifenol Total dari Daun Kadamba (Mitragyna speciosa Korth) Menggunakan Metode Choline Chloride-Sorbitol based Microwave Assisted Extraction

Proceeding of Mulawarman Pharmaceuticals Conferences ◽

10.25026/mpc.v10i1.377 ◽

2019 ◽

Vol 10 ◽

pp. 131-134

Author(s):

Ramila Hidayati ◽

Dewi Rahmawati ◽

Islamudin Ahmad

Keyword(s):

Choline Chloride ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Green Solvent ◽

Microwave Assisted ◽

Extract Solution ◽

Mitragyna Speciosa ◽

Assisted Extraction ◽

Total Extraction

This research was conducted to determine the influence of the use of the choline chloride-sorbitol based microwave assisted extraction (MAE) method against the total extraction of polyphenols from the leaf Kadamba (Mitragyna speciosa Korth). Simplisia extracted using solvent (green solvent) choline chloride-sorbitol with a ratio of 1:1; 1:2 and 1:3 g/g with sample and solvent ratio of 1:10 g/mL, microwave strength is 50% watt and performed for 10 minutes, the extract solution has been obtained and then done determination of total levels of polyphenols by using folin ciocalteu reagents and further measured its absorption with the UV-VIS spectrophotometer device at a wavelength of 770 nm. Based on the results of the study, obtained the levels of polyphenols from each comparison of solvent used against the sample of 334 mg of GAE/g samples (1:1 g/g solvent); 372 mg GAE/g samples (1:2 g/g solvents) and 299 mg of GAE/g samples (1:3 g/g solvents). Based on the results of the study can be concluded that the method of choline chloride-sorbitol based microwave assisted extraction (MAE) can be used to extract the polyphenols from Kadamba leaves easily, quickly and efficiently.

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