Anti-inflammatory and Anti-nociceptive Effects of Ethyl Acetate Fraction of Root Bark of Cassia sieberiana D. C. in Murine Models

Pharmacologia ◽  
2013 ◽  
Vol 4 (4) ◽  
pp. 301-310 ◽  
Author(s):  
Kofi Donkor ◽  
Laud N.K. Okine ◽  
Wonder K.M. Abotsi ◽  
Eric Woode
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Root Bark ◽  
Murine Models ◽  
Anti Inflammatory

scholarly journals Safety Profile, In Vitro Anti-Inflammatory Activity, and In Vivo Antiulcerogenic Potential of Root Barks from Annona senegalensis Pers. (Annonaceae)

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Kiessoun Konaté ◽  
Abdoudramane Sanou ◽  
Raïssa R. R. Aworet-Samseny ◽  
Fatiha Benkhalti ◽  
Oksana Sytar ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Mechanisms Of Action ◽  
Toxicity Tests ◽  
Root Bark ◽  
Dependent Manner ◽  
Inhibition Activity ◽  
Biological Tests ◽  
Bioactive Fraction ◽  
Anti Inflammatory

Annona senegalensis (Annonaceae) is a tropical shrub widely distributed in Burkina Faso. This plant is traditionally used as a medicine against many pathologies including typhoid fever, gastrointestinal disorders, ulcers, and inflammatory and infectious diseases. The present study was conducted to evaluate the anti-inflammatory and antiulcer properties of Annona senegalensis root bark extracts. Therefore, toxicity tests were first performed, followed by other biological tests. For this purpose, we first undertook to evaluate the toxicity tests before considering the other biological tests in a second step. The results showed that the extracted fractions had a significant effect for the different methods used (protein denaturation inhibition activity, hyaluronidase inhibition activity, and xanthine oxidase inhibition activity). However, of the extracted fractions used, the ethyl acetate fraction was the most anti-inflammatory fraction. The antiulcer activity was evaluated using the best bioactive fraction. The antiulcer effect of the ethyl acetate fraction may be due to both the reduction of gastric acid secretion and gastric cytoprotection. The results of this study also showed that the bioactive fraction reduced ethanol-induced ulceration and pyloric ligation in a dose-dependent manner, and at the highest dose (200 mg/kg), the effect was similar to that of the reference drug. In summary, the ethyl acetate fraction was found to have the best anti-inflammatory and antiulcerogenic activities. The ethyl acetate fraction at a dose of 200 mg/kg also showed a rather interesting level of cytoprotection. The anti-inflammatory and antiulcer activities could be due to the different secondary metabolites contained in the fractions extracted from Annona senegalensis, notably flavonoids, triterpenoids, steroids, saponins, and tannins. As the mechanisms of action are still little or not understood, we will consider in the future identifying the phytoconstituents and the mechanisms of action involved in the results.

Anti-inflammatory activity of ethyl acetate fraction of the seeds of Brucea Javanica

2013 ◽  
Vol 147 (2) ◽  
pp. 442-446 ◽  
Author(s):  
Jianhong Yang ◽  
Shucai Li ◽  
Caifeng Xie ◽  
Haoyu Ye ◽  
Huan Tang ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Inflammatory Activity ◽  
Brucea Javanica ◽  
Anti Inflammatory

scholarly journals Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages

Molecules ◽  
2016 ◽  
Vol 21 (11) ◽  
pp. 1452 ◽  
Author(s):  
Palanisamy Arulselvan ◽  
Woan Tan ◽  
Sivapragasam Gothai ◽  
Katyakyini Muniandy ◽  
Sharida Fakurazi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Signaling Pathway ◽  
Moringa Oleifera ◽  
Ethyl Acetate Fraction ◽  
Anti Inflammatory

scholarly journals Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Exerts Anti-Inflammatory Effects by Regulating miR-155 Expression and JNK Signaling Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yuan Xu ◽  
Xiao-Xia Chen ◽  
Yi-Xin Jiang ◽  
Dan-Dan Zhang
Keyword(s):  
Ethyl Acetate ◽  
Signaling Pathway ◽  
Ethyl Acetate Fraction ◽  
Equal Weight ◽  
Dependent Manner ◽  
Jnk Signaling ◽  
Scutellaria Barbata ◽  
Hedyotis Diffusa ◽  
Jnk Signaling Pathway ◽  
Anti Inflammatory

Hedyotis diffusa Willd and Scutellaria barbata D. Don (HDSB) were the core couplet in medicines that were commonly used for the purpose of anti-inflammation and anticancer treatments in China. However, biological properties of this couplet have not been fully elucidated. In this study, we screened fractions of HDSB for their anti-inflammatory activities and explored pertinent molecular mechanisms on murine macrophage RAW264.7 cell model. Ethyl acetate fraction from the aqueous extract of the couplet at equal weight ratio (EA11) showed the strongest inhibition of the nitrite accumulation in supernatant of RAW264.7 cells stimulated with lipopolysaccharide (LPS)/interferon-γ (IFN-γ). In addition, EA11 inhibited iNOS and IL-1β expression in a concentration-dependent manner while promoting the expression of HO-1 and PPAR-γ. Anti-inflammatory capability is most likely facilitated by its inhibitory effect on JNK signaling pathway and miR-155 expression. This study suggests that EA11 may be represented as a potential anti-inflammatory therapeutic candidate.

Antinociceptive and anti-inflammatory activities of ethyl acetate fraction from Zanthoxylum armatum in mice

Fitoterapia ◽  
2011 ◽  
Vol 82 (3) ◽  
pp. 347-351 ◽  
Author(s):  
Tao Guo ◽  
Yun-Xia Deng ◽  
Hui Xie ◽  
Chun-Yan Yao ◽  
Cheng-Cheng Cai ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Zanthoxylum Armatum ◽  
Anti Inflammatory

Anti-Inflammatory and Antinociceptive Activity of Flavonoids Isolated from Viscum album ssp. album

2006 ◽  
Vol 61 (1-2) ◽  
pp. 26-30 ◽  
Author(s):  
Didem Deliorman Orhan ◽  
Esra Küpeli ◽  
Erdem Yesilada ◽  
Fatma Ergun
Keyword(s):  
Ethyl Acetate ◽  
Inflammatory Diseases ◽  
Ethyl Acetate Fraction ◽  
Viscum Album ◽  
Antinociceptive Activity ◽  
Paw Edema ◽  
Writhing Test ◽  
Activity Assessment ◽  
Edema Model ◽  
Anti Inflammatory

Abstract Viscum album L. has been used in the indigenous systems of medicine for treatment of headache and some inflammatory diseases. In order to evaluate this information, antinociceptive and anti-inflammatory activities of the five flavonoids (5,7-dimethoxy naringenin or 4′,6′- dimethoxy chalcononaringenin) derivatives, isolated from the ethyl acetate fraction of the extract from V. album ssp. album, were investigated, namely 5,7-dimethoxy-flavanone-4′-O- β-ᴅ-glucopyranoside (1), 2′-hydroxy-4′,6′-dimethoxy-chalcone-4-O-β-ᴅ-glucopyranoside (2), 5,7-dimethoxy-flavanone-4′-O-[2″-O-(5‴-O-trans-cinnamoyl)-β-ᴅ-apiofuranosyl]-β-ᴅ-glucopyranoside (3), 2′-hydroxy-4′,6′-dimethoxy-chalcone-4-O-[2″-O-(5‴-O-trans-cinnamoyl)-β-ᴅ-apiofuranosyl]- β-ᴅ-glucopyranoside (4), 5,7-dimethoxy-flavanone-4′-O-[β-d-apiofuranosyl- (152)]-β-ᴅ-glucopyranoside (5). For the antinociceptive activity assessment the p-benzoquinone- induced writhing test and for the anti-inflammatory activity the carrageenan-induced hind paw edema model in mice were used. The ethyl acetate fraction in a dose of 250 mg/kg as well as compounds 2 and 5 in a 30 mg/kg dose were shown to possess remarkable antinociceptive and anti-inflammatory activities per os without inducing any apparent acute toxicity as well as gastric damage

scholarly journals Evaluations and Mechanistic Interrogation of Natural Products Isolated From Paeonia suffruticosa for the Treatment of Inflammatory Bowel Disease

2021 ◽  
Vol 12 ◽  
Author(s):  
Kun-Chang Wu ◽  
Der-Yen Lee ◽  
Jeh-Ting Hsu ◽  
Chi-Fang Cheng ◽  
Joung-Liang Lan ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Therapeutic Potential ◽  
Ethyl Acetate Fraction ◽  
Root Bark ◽  
Dependent Manner ◽  
Paeonia Suffruticosa ◽  
Inflammatory Bowel ◽  
Anti Inflammatory ◽  
The Impact

Mu Dan Pi (MDP), a traditional Chinese medicine derived from the root bark of Paeonia suffruticosa Andrews, is used to treat autoimmune diseases due to its anti-inflammatory properties. However, the impact of MDP on inflammatory bowel disease (IBD) and its principal active compounds that contribute to the anti-inflammatory properties are uncertain. Thus, this study systemically evaluated the anti-inflammatory effects of fractionated MDP, which has therapeutic potential for IBD. MDP fractions were prepared by multistep fractionation, among which the ethyl acetate-fraction MDP5 exhibited the highest potency, with anti-inflammatory activity screened by the Toll-like receptor (TLR)-2 agonist, Pam3CSK4, in a cell-based model. MDP5 (at 50 μg/ml, p < 0.001) significantly inhibited nuclear factor kappa-B (NF-κB) reporters triggered by Pam3CSK4, without significant cell toxicity. Moreover, MDP5 (at 10 μg/ml) alleviated proinflammatory signaling triggered by Pam3CSK4 in a dose-dependent manner and reduced downstream IL-6 and TNF-α production (p < 0.001) in primary macrophages. MDP5 also mitigated weight loss, clinical inflammation, colonic infiltration of immune cells and cytokine production in a murine colitis model. Index compounds including paeoniflorin derivatives (ranging from 0.1 to 3.4%), gallic acid (1.8%), and 1,2,3,4,6-penta-O-galloyl-β-D-glucose (1.1%) in MDP5 fractions were identified by LC-MS/MS and could be used as anti-inflammatory markers for MDP preparation. Collectively, these data suggest that MDP5 is a promising treatment for IBD patients.

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