scholarly journals The determination of the degree of serological relationship among four carlaviruses using an immunoelectron microscope-decoration technique and cross-absorption tests

2013 ◽  
Vol 41 (1) ◽  
pp. 5-15
Author(s):  
Marek S. Szyndel
Keyword(s):  
Serological Relationship ◽  
Distant Relationship ◽  
Cross Absorption ◽  
Decoration Technique ◽  
Antigenic Fraction

A very close serological relationship was found between CVB and PVS, while only a distant relationship was shown between CVB and CLV. Each of these viruses had its own characteristic major "antigenic fraction", distinguishing it from the others, and small "antigenic fraction" common to all four. The antiserum to CLV was shown to be the richest in various antigenic fractions, antisera against PVM and PVS had the same number of fractions but of different specificity and CVB-antiserum had the relatively smallest number of fractions.

scholarly journals The use of an immunoelectron microscope-decoration technique to observe serological relationship of carlaviruses

2013 ◽  
Vol 41 (1) ◽  
pp. 17-26
Author(s):  
Marek S. Szyndel
Keyword(s):  
Serological Relationship ◽  
Decoration Technique ◽  
Relationship Of ◽  
Antibody Coating

An immunoelectron microscope-decoration technique was used to define serological relationships among carlaviruses (5 viruses and 9 antisera). The degree of relationship among these viruses was assessed on the basis of the intensity of antibody coating when the viruses were decorated with heterologous antibodies. A relationship was observed among PVM, PVS, CLV, CVB, LilMV, HoLV, HoMV and between WPSV and LilMV. PopMV was not related serologically to any of these viruses. The PVS particles were occasionally surrounded by an external spiral of an unidentified protein material.

scholarly journals The serological relationship betweenBrucellaspp.,Yersinia enterocoliticaserotype IX andSalmonellaserotypes of Kauffmann-White group N

1975 ◽  
Vol 75 (1) ◽  
pp. 151-171 ◽  
Author(s):  
M. J. Corbel
Keyword(s):  
Complement Fixation ◽  
Fluorescent Antibody ◽  
High Titre ◽  
Antigenic Structure ◽  
Antigenic Determinants ◽  
Serological Relationship ◽  
Homologous Strain ◽  
Cross Absorption ◽  
Salmonella Serotypes ◽  
Monospecific Antisera

SUMMARYThe serological relationship betweenBrucellaspp.,Yersinia enterocoliticaIX, and the group N salmonella serotypesS. godesberg, S. landau, S. morehead, S. neusdorf, S. soerengaandS. urbanawas examined using agglutination, antiglobulin, complement fixation, immunodiffusion and fluorescent antibody methods.Antisera to the group N salmonella serotypes all reacted to significant titres in agglutination and complement fixation, but not antiglobulin or immunodiffusion tests with smooth brucella antigens. These antisera also reacted in agglutination, but not antiglobulin, tests withY. enterocoliticaIX. They did not react significantly in any tests with rough brucella antigens.Conversely, antisera to smoothBrucellaspp. agglutinated group N salmonellas to low titre andY. enterocoliticaIX to titres similar to those given against the homologous strain. Antiserum toY. enterocoliticaIX on the other hand reacted with smooth brucella antigens to high titre in agglutination, complement fixation and antiglobulin tests, and with the group N salmonella antigens to substantial titres in agglutination tests.In direct fluorescent antibody tests, smoothBrucellastrains andY. enterocoliticaIX reacted strongly with FITC-labelled antibody toBr. abortuswhereas the group N salmonella strains reacted weakly.In tests with monospecific antisera to the A and M determinants ofBr. abortusandBr. melitensisrespectively,Y. enterocoliticaIX reacted only with the antiserum to the A determinant whereas group N salmonellas reacted to low titre with both A and M antisera.The results of cross-absorption tests confirmed this relationship and suggested that the O30 antigens of group N salmonella serotypes contained antigenic determinants similar to, but not identical with, the antigenic structure shared by smoothBrucellaspp. andY. enterocoliticaIX.

THE PROBLEM OF SPECIFICITY IN SEROLOGIC DETERMINATION OF HUMAN CHORIONIC GONADOTROPHIN

1963 ◽  
Vol 42 (4) ◽  
pp. 485-497 ◽  
Author(s):  
Sam Brody ◽  
Gun Carlström
Keyword(s):  
Immune System ◽  
Complement Fixation ◽  
Body Fluids ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Serological Relationship ◽  
Relationship Of ◽  
Urine And Serum ◽  
Serologic Specificity

ABSTRACT Rabbit antisera, prepared against a commercial, highly purified preparation of human chorionic gonadotrophin, have been examined with regard to their serologic specificity. Analyses were carried out using procedures of immunodiffusion and complement fixation. Heterogeneity of the immune system is confirmed, and the distribution and serological relationship of unspecific antigens in urine and serum examined. The effect of various methods on the elimination of unspecific antibodies is shown. The implications of these findings on the immunoassay of human chorionic gonadotrophin in body fluids are discussed.

scholarly journals Differentiation of Rhizoctonia spp. Based on their antigenic properties

2002 ◽  
Vol 47 (2) ◽  
pp. 137-147
Author(s):  
Ivana Vico ◽  
Branka Krstic ◽  
Natasa Dukic
Keyword(s):  
Anastomosis Group ◽  
Serological Relationship ◽  
Binucleate Rhizoctonia ◽  
Anastomosis Groups ◽  
Potato Plants ◽  
Antigenic Properties ◽  
Cross Absorption ◽  
Fusarium Sp ◽  
Polyclonal Antisera ◽  
Taxonomic Groups

Antigenic properties and serological relationship was investigated in binucleate and multinucleate Rhizoctonia spp. isolates from strawberries soybean, alfalfa and potato plants from Serbia, from Spain, anastomosis group testers and in strawberry roots inoculated with binucleate Rhizoctonia AG A and AG I. Two polyclonal antisera, unabsorbed and cross absorbed, were used in dot-immunobinding assay for these investigations. Antisera were produced against mycelial antigens of two isolates, which belong to different anastomosis groups (AG) of binucleate Rhizoctonia - AG A and AG I. Both unabsorbed antisera reacted positively with all tested Rhizoctonia spp. isolates, and the reaction was absent with control isolates (Pythium sp. Agaricus sp. and Fusarium sp). The results prove a close serological relationship among Rhizoctonia spp. isolates, and diversity between Rhizoctonia spp. and isolates from different taxonomic groups. Also, both unabsorbed antisera reacted with higher intensity with closely related antigens (belonging to the same AG) than with ones from another AG of binucleate Rhizoctonia or R. solani (multinucleate Rhizoctonia). After cross absorption specificity of the antisera was enhanced, especially with the antiserum raised against mycelial proteins of binucleate Rhizoctonia AG I. This antiserum reacted positively only with antigens from the same AG, after cross absorption with antigens from AG A of binucleate Rhizoctonia and from R. solani AG 2-2. It proved to be specific to AG I of binucleate Rhizoctonia, and able to differentiate isolates of this AG from others. In this way the serological homology among isolates of one AG was proven, and also the diversity among isolates which belong to different AGs of binucleate Rhizoctonia as well as isolates of R. solani.

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

Distance to SN 1987A and the LMC

1999 ◽  
Vol 190 ◽  
pp. 549-554
Author(s):  
Nino Panagia
Keyword(s):  
Angular Size ◽  
Large Magellanic Cloud ◽  
Magellanic Cloud ◽  
Light Curves ◽  
Distance Modulus ◽  
Absolute Size ◽  
Sn 1987A

Using the new reductions of the IUE light curves by Sonneborn et al. (1997) and an extensive set of HST images of SN 1987A we have repeated and improved Panagia et al. (1991) analysis to obtain a better determination of the distance to the supernova. In this way we have derived an absolute size of the ringRabs= (6.23 ± 0.08) x 1017cm and an angular sizeR″ = 808 ± 17 mas, which give a distance to the supernovad(SN1987A) = 51.4 ± 1.2 kpc and a distance modulusm–M(SN1987A) = 18.55 ± 0.05. Allowing for a displacement of SN 1987A position relative to the LMC center, the distance to the barycenter of the Large Magellanic Cloud is also estimated to bed(LMC) = 52.0±1.3 kpc, which corresponds to a distance modulus ofm–M(LMC) = 18.58±0.05.

International determination of the total motion of the pole

1961 ◽  
Vol 13 ◽  
pp. 29-41
Author(s):  
Wm. Markowitz
Keyword(s):  
Secular Motion ◽  
The Future

A symposium on the future of the International Latitude Service (I. L. S.) is to be held in Helsinki in July 1960. My report for the symposium consists of two parts. Part I, denoded (Mk I) was published [1] earlier in 1960 under the title “Latitude and Longitude, and the Secular Motion of the Pole”. Part II is the present paper, denoded (Mk II).

Time and Latitude in South Africa

1972 ◽  
Vol 1 ◽  
pp. 27-38
Author(s):  
J. Hers
Keyword(s):  
South Africa ◽  
Cape Town ◽  
Astronomical Observations ◽  
Royal Observatory ◽  
The Republic ◽  
Astronomical Determination ◽  
Limited Accuracy ◽  
Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

Large-scale Motion of Solar Filaments

2000 ◽  
Vol 179 ◽  
pp. 205-208
Author(s):  
Pavel Ambrož ◽  
Alfred Schroll
Keyword(s):  
Magnetic Flux ◽  
Proper Motion ◽  
Time Scale ◽  
Large Scale ◽  
Accuracy Of Measurements ◽  
Solar Filaments ◽  
General Movement ◽  
Scale Motion ◽  
Velocity Scatter

AbstractPrecise measurements of heliographic position of solar filaments were used for determination of the proper motion of solar filaments on the time-scale of days. The filaments have a tendency to make a shaking or waving of the external structure and to make a general movement of whole filament body, coinciding with the transport of the magnetic flux in the photosphere. The velocity scatter of individual measured points is about one order higher than the accuracy of measurements.

Practical Realization of a Reference System for Earth Dynamics by Satellite Methods

1975 ◽  
Vol 26 ◽  
pp. 341-380 ◽  
Author(s):  
R. J. Anderle ◽  
M. C. Tanenbaum
Keyword(s):  
Reference Frame ◽  
Polar Motion ◽  
Pole Position ◽  
Control Points ◽  
Significant Information ◽  
Crustal Motion ◽  
Average Reference ◽  
Future Data ◽  
Existing Data

AbstractObservations of artificial earth satellites provide a means of establishing an.origin, orientation, scale and control points for a coordinate system. Neither existing data nor future data are likely to provide significant information on the .001 angle between the axis of angular momentum and axis of rotation. Existing data have provided data to about .01 accuracy on the pole position and to possibly a meter on the origin of the system and for control points. The longitude origin is essentially arbitrary. While these accuracies permit acquisition of useful data on tides and polar motion through dynamio analyses, they are inadequate for determination of crustal motion or significant improvement in polar motion. The limitations arise from gravity, drag and radiation forces on the satellites as well as from instrument errors. Improvements in laser equipment and the launch of the dense LAGEOS satellite in an orbit high enough to suppress significant gravity and drag errors will permit determination of crustal motion and more accurate, higher frequency, polar motion. However, the reference frame for the results is likely to be an average reference frame defined by the observing stations, resulting in significant corrections to be determined for effects of changes in station configuration and data losses.

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