scholarly journals The amino acid composition of cotyledons, testa, embryo and protein fractions of bean seeds

2013 ◽  
Vol 41 (1) ◽  
pp. 69-75
Author(s):  
Ryszard Kosson

The contribution of cotyledons, embryo and testa to the whole seed, was analyzed in the bean cultivar, Wiejska. The total nitrogen content and amino acid composition of morphological parts of the seed were determined. The average amino acid composition of globulins and albumins and the content of free amino acids in seeds of six Polish cultivars were estimated as well. It was found that the embryo contained the highest quantity of total nitrogen and the lowest of protein nitrogen. The exogenous amino acid content in the embryo was higher than in cotyledons and testa. Both albumins and globulins were shown to contain 42% exogenous amino acids. The content of methionine - the first limiting amino acid of bean proteins - did not exceed 0.30% of the total amino acid content in albumins and globulins. Free glutamic and aspartic acids made up more than 60% of the total free amino acids.

1981 ◽  
Vol 8 (2) ◽  
pp. 131-133 ◽  
Author(s):  
David C. H. Hsi ◽  
Clyde T. Young ◽  
Melchor Ortiz

Abstract Two Valencia peanut (Arachis hypogaea L.) cultivars, New Mexico Valencia A and New Mexico Valencia C, were grown at Arch and Los Lunas, N. M. All peanuts were grown under either sprinkler or furrow irrigation. Samples were obtained following harvest, hydrolyzed and analyzed in Raleigh, N. C. for amino acid content. With samples from Arch, no variety by planting date effects were noted for amino acid composition. With samples from Los Lunas, however, significant variety by planting date effects were noted for glutamic acid, glycine, methionine, isoleucine, and leucine. Planting date effects were found only for glycine at the Los Lunas location and phenylalanine at both locations. Significant variety differences were found for methionine, tyrosine, phenylalanine, and lysine at the Arch location and for glycine, methionine, phenylalanine, and lysine at the Los Lunas location. A significant year effect, although small, was noted for about half of the amino acids (aspartic acid, serine, glutamic acid, proline, glycine, tyrosine, phenylalanine, arginine, and the sum of all amino acids) studied at the Arch location. Data at Los Lunas were observed only during the second year. The amino acid content agrees closely with that previously reported for other types of peanuts except for a 100% higher level of cystine found in this study.


1964 ◽  
Vol 10 (5) ◽  
pp. 623-631 ◽  
Author(s):  
C. Ramirez ◽  
J. J. Miller

During 6-day exposures of cells of Saccharomyces cerevisiae to acetate sporulation medium, the content of free amino acids declined to approximately one-third of that of vegetative cells, but proline was exceptional in that it increased conspicuously in amount. The content of combined amino acids also diminished to about one-third, ammonia was evolved, and amino acids (not including proline) passed out of the cells into the medium. When dihydroxyacetone replaced acetate in the sporulation medium, the results were similar except that the decline in content of free and combined amino acids was much greater, more ammonia was evolved, and only very small amounts of amino acids could be detected in the medium. Transfer of sporulated cells to growth medium led to an increase in the pool of free amino acids, except for proline, which declined in amount.In two other species of Saccharomyces the free proline content also increased on exposure to sporulation medium, but in Schizosaccharomyces pombe and Torulopsis famata no such increase was observed.


1973 ◽  
Vol 52 (3) ◽  
pp. 418-419 ◽  
Author(s):  
Pan H. Papanayotou ◽  
Joanna Dozi-Vassiliades ◽  
Anast. Kovatsis

By the use of thin layer chromatographic techniques and exchange column chromatography, 20 free amino acids were found in human saliva. No qualitative or semiquantitative differences in the amino acid content were observed among caries-susceptible and caries-resistant individuals 20 to 30 years old.


2019 ◽  
pp. 135-143
Author(s):  
Evgeniy Nikolaevich Sechin ◽  
Oleg Anatolyevich Marakaev ◽  
Gavriil Borisovich Gavrilov

Amino acid composition of aboveground and underground vegetative organs of Dactylorhiza maculata (L.) Soó (Orchidaceae), one of the representatives of the tuberoid species of orchids growing under the natural conditions of the center of European Russia, was detected using the method of zone capillary electrophoresis. The presence of 15 amino acids in the plant material, nine of them are «essential» (lysine, phenylalanine, histidine, leucine, isoleucine, methionine, valine, threonine, tryptophan) was established. The highest total amino acid content is characteristic of the leaves, the smallest for the old (wintered) caulorrhizous tuberoids. Among the identified amino acids in the plant material of D. maculata, the maximum total content is of leucine, the minimum are of tryptophan and methionine. The vegetative organs are also rich in alanine, arginine, valine and phenylalanine. The total content of amino acids in young caulorrhizous tuberoids is 38% higher than that in old storage organs. These differences are most pronounced for arginine, which is probably due to the spare function of this amino acid, containing more than 30% nitrogen. The got data indicate the promise of further studies of the amino acid composition of D. maculata and can characterize this species as a source of medicinal valuable substances with a broad spectrum of pharmacological activity.


2009 ◽  
Vol 5 (2) ◽  
pp. 194-196 ◽  
Author(s):  
Stuart Warwick ◽  
Karim Vahed ◽  
David Raubenheimer ◽  
Stephen J Simpson

Nuptial gifts that are manufactured by the male are found in numerous insect species and some spiders, but there have been very few studies of the composition of such gifts. If, as has been proposed recently, nuptial gifts represent sensory traps, males will be selected to produce gifts that are attractive to females but such gifts will not necessarily provide the female with nutritional benefits (the ‘Candymaker’ hypothesis). We examined the free amino acid content of the spermatophylax of the cricket Gryllodes sigillatus (Orthoptera: Gryllidae) using high performance liquid chromatography (HPLC). The spermatophylax (dry weight) consisted of approximately 7 per cent free amino acids. The free amino acid composition was highly imbalanced, with a low proportion of essential amino acids (18.7%) and a high proportion of proline and glycine. The main free amino acids found in the spermatophylax appeared to act as phagostimulants: the duration of feeding on artificial gels by females was positively related to the free amino acid content of the gels. The results therefore suggest that males use free amino acids to ‘sweeten’ a relatively low-value food item. A possible function of glycine in inhibiting female movement is also proposed.


1956 ◽  
Vol 9 (3) ◽  
pp. 400 ◽  
Author(s):  
RH Hackman

Blood from the larval, prepupal, and early pupal stages of Calliphora augttr' (F.) contains the same 18 free amino acids. In addition, hydroxyproline is present in larval and prepupal blood. The quantitative differenccs in the concent,ratiolls of these amino acids arc reported. Larval blood has the highest free amino acid content (6'6 mgjml) followcd by early pupal blood (4'6 mgjml) and prepnpal blood (3'3 mgjml). As the larva matures to the prepupa, the greatest decreases occm in the concentrations in the blood of alanine, glutamic acid, glutamine, glycine, proline, and tyrosine. The metabolic significance of these changes are discussed.


Author(s):  
Yu. E. Razvodovsky ◽  
V. Yu. Smirnov ◽  
I. N. Semenenya

The effects of complex compositions, containing branched-chain amino acids (BCAA), taurine and tryptophan, on the pool of free amino acids in the liver of rats were studied under the conditions of subchronic alcohol intoxication (SHAI). It was established that SHAI led to the decreased levels of treonine, lysine, oxyproline, arginine, b-alanine, as well as the depletion of the pool of irreplaceable amino acids in the liver of rats. Administration of the composition of BCAA and taurine was found to normalize the ratio of replaceable irreplaceable amino acids, the ratio of glycogenic and ketogenic amino acids, to activate the reaction of nitrogen utilization, and to increase Fisher’s index. The effects of the composition, containing BCAA, taurine and tryptophan, were similar to those of amino acid composition that did not contain tryptophan.


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