scholarly journals Effect of systemic fungicides on the efficacy of Pseudomonas aeruginosa (Schroeter) migula in the control of root-infecting fungi of wheat

2013 ◽  
Vol 53 (2) ◽  
pp. 39-46
Author(s):  
I. A. Siddiqui ◽  
S. S. Shaukat ◽  
S. Ehteshamul-Haque ◽  
S. A. Khan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Plant Growth ◽  
Grain Yield ◽  
Macrophomina Phaseolina ◽  
Effective Control ◽  
Bacterial Survival ◽  
Thiophanate Methyl ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Wheat Seeds

Effect of various fungicides on the efficacy of <i>Pseudomonas aeruginosa</i>, a plant growth-promoting rhizobacterium in the control of root-infecting fungi such as <i>Macrophomina phaseolina, Fusarium oxysporum</i> and <i>F.solani</i> on four different varieties of wheat was evaluated under field conditions. Bayleton (a.i. triadimephon), Bavistin (a.i. carbendazym) and Topsin-M (a.i. thiophanate-methyl) reduced bacterial survival on wheat seeds whereas Benlate (a.i. benomyl) was not effective in this respect. <i>P.aeruginosa</i> used in combination with Benlate showed effective control of soilborne root-infecting fungi along with the enhancement of growth and grain yield of wheat.

scholarly journals Effect of crustacean chitin on the efficacy of plant growth promoting bacteria in the control of root infecting fungi of sunflower and chickpea

2013 ◽  
Vol 53 (1) ◽  
pp. 5-12 ◽  
Author(s):  
Viqar Sutana ◽  
Syed Ehteshamul-Haque ◽  
Jehan Ara ◽  
Rashid Qasim ◽  
Abdul Ghaffar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Plant Growth ◽  
Fusarium Solani ◽  
Soil Amendment ◽  
Macrophomina Phaseolina ◽  
Plant Growth Promoting Bacteria ◽  
Fresh Weight ◽  
Plant Growth Promoting ◽  
Growth Promoting

Soil amendment with crustacean chitin used alone or with <em>Pseudomonas aeruginosa Bacillus subtilis</em> significantly (p<0,05) reduced infection of <em>Rhizoctonia solani Fusarium solani</em> on sunflower and <em>R.solani</em> on chickpea. Crab chitin used alone or with <em>P.aeruginosa</em> or <em>B.subtilis</em> completely controlled the infection of <em>Macrophomina phaseolina</em> on chickpea. Prawn or shrimp powder used 1% w/w of soil was found phytotoxic on chickpea but not on sunflower. Maximum fresh weight of shoot was produced by <em>P.aeruginosa</em> used with shrimp powder in sunflower and with crab chitin in chickpea. <em>P.aeruginosa</em> produced greater plant height in chickpea used with shrimp chitin.

scholarly journals Plant growth promoting endophyte Burkholderia contaminans NZ antagonizes phytopathogen Macrophomina phaseolina through melanin synthesis and pyrrolnitrin inhibition

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257863
Author(s):  
Nazia R. Zaman ◽  
Umar F. Chowdhury ◽  
Rifath N. Reza ◽  
Farhana T. Chowdhury ◽  
Mrinmoy Sarker ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Genome Mining ◽  
Gene Clusters ◽  
Macrophomina Phaseolina ◽  
Melanin Synthesis ◽  
Burkholderia Contaminans ◽  
Plant Growth Promoting ◽  
Growth Promoting

The endophytic bacterium Burkholderia contaminans NZ was isolated from jute, which is an important fiber-producing plant. This bacterium exhibits significant growth promotion activity in in vivo pot experiments, and like other plant growth-promoting (PGP) bacteria fixes nitrogen, produces indole acetic acid (IAA), siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. B. contaminans NZ is considered to exert a promising growth inhibitory effect on Macrophomina phaseolina, a phytopathogen responsible for infecting hundreds of crops worldwide. This study aimed to identify the possibility of B. contaminans NZ as a safe biocontrol agent and assess its effectiveness in suppressing phytopathogenic fungi, especially M. phaseolina. Co-culture of M. phaseolina with B. contaminans NZ on both solid and liquid media revealed appreciable growth suppression of M. phaseolina and its chromogenic aberration in liquid culture. Genome mining of B. contaminans NZ using NaPDoS and antiSMASH revealed gene clusters that displayed 100% similarity for cytotoxic and antifungal substances, such as pyrrolnitrin. GC-MS analysis of B. contaminans NZ culture extracts revealed various bioactive compounds, including catechol; 9,10-dihydro-12’-hydroxy-2’-methyl-5’-(phenylmethyl)- ergotaman 3’,6’,18-trione; 2,3-dihydro-3,5- dihydroxy-6-methyl-4H-pyran-4-one; 1-(1,6-Dioxooctadecyl)- pyrrolidine; 9-Octadecenamide; and 2- methoxy- phenol. These compounds reportedly exhibit tyrosinase inhibitory, antifungal, and antibiotic activities. Using a more targeted approach, an RP-HPLC purified fraction was analyzed by LC-MS, confirming the existence of pyrrolnitrin in the B. contaminans NZ extract. Secondary metabolites, such as catechol and ergotaman, have been predicted to inhibit melanin synthesis in M. phaseolina. Thus, B. contaminans NZ appears to inhibit phytopathogens by apparently impairing melanin synthesis and other potential biochemical pathways, exhibiting considerable fungistatic activity.

scholarly journals Isolation and Molecular Characterization of a Model Antagonistic Pseudomonas aeruginosa Divulging In Vitro Plant Growth Promoting Characteristics

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Bushra Uzair ◽  
Rehana Kausar ◽  
Syeda Asma Bano ◽  
Sammer Fatima ◽  
Malik Badshah ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Plant Growth ◽  
Growth Promotion ◽  
Gene Bank ◽  
Accession Number ◽  
Bacterial Strains ◽  
Rhizopus Microsporus ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Gene Bank Accession

The use of microbial technologies in agriculture is currently expanding quite rapidly with the identification of new bacterial strains, which are more effective in promoting plant growth. In the present study 18 strains of Pseudomonas were isolated from soil sample of Balochistan coastline. Among isolated Pseudomonas strains four designated as SP19, SP22, PS24, and SP25 exhibited biocontrol activities against phytopathogenic fungi, that is, Rhizopus microsporus, Fusarium oxysporum, Aspergillus niger, Alternaria alternata, and Penicillium digitatum; PS24 identified as Pseudomonas aeruginosa by 16srRNA gene bank accession number EU081518 was selected on the basis of its antifungal activity to explore its potential as plant growth promotion. PS24 showed multiple plant growth promoting attributes such as phosphate solubilization activity, indole acetic acid (IAA), siderophore, and HCN production. In order to determine the basis for antifungal properties, antibiotics were extracted from King B broth of PS24 and analyzed by TLC. Pyrrolnitrin antibiotic was detected in the culture of strain PS24. PS24 exhibited antifungal activities found to be positive for hydrogen cyanide synthase Hcn BC gene. Sequencing of gene of Hcn BC gene of strain PS24 revealed 99% homology with the Pseudomonas aeruginosa strain PA01. The sequence of PS24 had been submitted in gene bank accession number KR605499. Ps. aeruginosa PS24 with its multifunctional biocontrol possessions can be used to bioprotect the crop plants from phytopathogens.

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