Characterization of plant growth promoting rhizobacterial strain pseudomonas aeruginosa MCC 3877 with special reference to indole acetic acid (IAA) production

2019 ◽  
Vol 10 (3) ◽  
Author(s):  
BANERJEE SOUMI ◽  
MUKHERJEE BIYAS ◽  
DUTTA SIKHA
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Acetic Acid ◽  
Plant Growth ◽  
Special Reference ◽  
Indole Acetic Acid ◽  
Iaa Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Molecular Biotyping, Phylogenetic Analysis of Indole Acetic Acid Producing Rhizobium Sp. CU8 and Co-inhabitant Non-rhizobial Nodule Associated Bacteria From Mimosa Pudica L.

2021 ◽  
Author(s):  
Maya R. ◽  
YUSUF AKKARA
Keyword(s):  
Phylogenetic Analysis ◽  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Evolutionary Significance ◽  
Mimosa Pudica ◽  
Associated Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract The interaction between rhizobia and other nodule associated bacteria assist to mitigate nutrient stress in leguminous plants by fixing atmospheric nitrogen and synthesizing plant growth regulators. Beneficial effects of microbial inoculants emphasize the need for further research and their use in modern agriculture. The present work describes the isolation, identification, plant growth promoting properties and phylogenetic analysis of nodule associated bacteria from Mimosa pudica L. Isolation and characterization of nodule associated bacteria were done according to standard procedures. Molecular characterization of the isolates was performed using 16S ribosomal RNA. Plant growth promoting ability was analyzed by quantifying the levels of Indole acetic acid. Evolutionary distance and relatedness was analyzed using neighbor joining method. Rhizobium sp. CU8 and three other co-resident non-rhizobial nodule associated bacteria (Bacillus cereus MY5, Ralstonia pickettii MY1 and Lactococcus lactis MY3) exhibiting nitrogen fixation, plant growth promotion and other potential microbial activities were characterized. Phylogenetic analysis revealed the genetic relatedness and evolutionary significance of Rhizobium sp. CU8 and other co-inhabitant non-rhizobial nodule associated bacteria from M. pudica. Present study identified the four isolates as potential biofertilizers due to their nitrogen fixing and growth promoting characteristics. L. lactis MY3 is the first co-resident nitrogen fixer and plant growth promoter reported from the root nodules of M. pudica.

scholarly journals Isolation and characterization of novel plant growth-promoting rhizobacteria (PGPR) isolates from tomato (Solanum lycopersicum L.) rhizospherical soil: A novel IAA producing bacteria

2021 ◽  
Vol 48 (2) ◽  
Author(s):  
Narjes H. Dashti ◽  
◽  
Nedaa Y. Ali Al-Sarraf ◽  
Vineetha M. Cherian ◽  
Magdy S. Montasser ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Indole Acetic Acid ◽  
Plant Growth Promoting Rhizobacteria ◽  
Nucleotide Sequence Analysis ◽  
Iaa Production ◽  
Solanum Lycopersicum L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Indole acetic acid (IAA) production is one of the distinctive traits of plant growth-promoting rhizobacteria (PGPR). Ten different rhizobacterial strains were isolated from the roots of tomato crops (Solanum lycopersicum L.), grown in Kuwait, and then screened for their plant growth-promoting abilities, with emphasis on IAA production. The isolated strains were identified by 16S partial r-RNA sequencing and further characterized by conventional biochemical tests. Based on the nucleotide sequence analysis and homology of the isolates, a phylogenetic tree was constructed. The plant growth-promoting abilities of the strains were determined by testing for ammonium production, hydrogen cyanide and acid production, growth in nitrogen-free media, phosphate solubilization, and IAA production. The potential IAA PGPRs were re-applied onto the rhizosphere of green-house grown tomato plants to determine their effects on tomato growth. The results have indicated that all isolated strains were able to produce indole acetic acid even without the presence of a tryptophan precursor.

Influence of Plant Growth Promoting Rhizobium on Growth of Pigeon Pea [Cajanus cajan (L.) Millsp.] 

2021 ◽  
Author(s):  
H.A. Harshitha ◽  
Geeta Goudar
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Functional Characterization ◽  
Pigeon Pea ◽  
Iaa Production ◽  
Northern District ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Biological Nitrogen

Background: Symbiotic nitrogen fixation plays a major role in the production of million tons of total biological nitrogen. The Rhizobium not only fixes the nitrogen but also produces plant growth promoting hormones. In this view, the present study was undertaken to characterize the native Rhizobium isolates for their functional properties and evaluate on pigeon pea for their plant growth promotional activities. Methods: The present investigation was carried out during the year 2018-19 at UAS, Dharwad. Five of the native isolates obtained from Northern district of Karnataka were subjected for functional characterization using standard methods. The isolates were also tested for their plant growth promotional activities.Result: The IAA production in the isolates was ranged from 20.50 to 22.85 mg IAA/ml of broth and GA production from 13.40 to 14.51 mg/25ml of broth. ACC deamination activity in the isolates was ranged from 57.50 to 75.5 nmoles of α-ketobutyrate/mg/h of broth. All the isolates exhibited their ability to solubilize P and Zn. Out of ten isolates, four isolates exhibited potential to inhibit mycelial growth of Fusarium oxysporum f. sp. udum and per cent inhibition was ranged from 48.75 to 73.75. All the isolates were found to be positive for siderophore and HCN production. The isolate AMVPR98 performed better with respect to all the growth parameters of pigeon pea. Molecular characterization of isolate AMVPR98 revealed 99% similarity with Rhizobium pusense strain AB3.

Effect of Pseudomonas aeruginosa ZGKD2 on the Growth of Amorpha fruticosa L in Coal Gangue

2013 ◽  
Vol 807-809 ◽  
pp. 2023-2026
Author(s):  
Yu Xiu Zhang ◽  
Pei Li Shi ◽  
Qian Zhang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Acetic Acid ◽  
Plant Growth ◽  
Root Length ◽  
Phosphate Solubilization ◽  
Fresh Weight ◽  
Amorpha Fruticosa ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

The cadmium-resistant Pseudomonas aeruginosa strain ZGKD2 was isolated from gangue pile of coal area. Production of siderophores, indole-3-acetic acid (IAA) and the solubilization of phosphate were observed in the strain. Two types of siderophores were identified by UV spectrophotometer. The highest production of IAA and phosphate solubilization were 2.0 ug/mL and 7.2 ug/mL. The root length, plant height and fresh weight of Amorpha fruticosa L in the substrates of Coal gannue and losses were promoted after inoculation with ZGKD2. These data indicated that Pseudomonas aeruginosa strain ZGKD2 was a plant growth-promoting bacterial (PGPB).

scholarly journals Bambara Groundnut Rhizobacteria Antimicrobial and Biofertilization Potential

2020 ◽  
Author(s):  
Caroline F. Ajilogba ◽  
Olubukola O. Babalola ◽  
Patrick Adebola ◽  
Rasheed Adeleke
Keyword(s):  
Acetic Acid ◽  
Food Security ◽  
Plant Growth ◽  
Hydrogen Cyanide ◽  
Indole Acetic Acid ◽  
Soil Samples ◽  
Bambara Groundnut ◽  
Dual Culture ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractBambara groundnut, an underutilized crop has been proved to be an indigenous crop in Africa with the potential for food security. The rhizosphere of Bambara groundnut like other legumes contains several important bacteria that have not been explored for their plant growth-promoting properties. The aim of this research was to determine the potentials of rhizobacteria from Bambara groundnut soil samples as either biofertilizer or biocontrol agents or both to help provide sustainable agriculture in Africa and globally. Analyses of Bambara groundnut rhizospheric soil samples included chemical analysis such as nitrogen content analysis using extractable inorganic nitrogen method as well as cation exchangeable capacity using ammonium acetate method. Plant growth-promoting properties of isolated rhizobacteria tested include indole acetic acid, hydrogen cyanide, phosphate solubilization, 1-aminocyclopropane-1-carboxylate and ammonia production activities using standard methods. In addition, antifungal assay dual culture method was used to analyze the biocontrol properties of the isolates. Phylogenetic analysis using 16S rRNA was also carried out on the isolates. Isolated rhizobacteria from bambara groundnut rhizosphere were cultured. All the isolates were able to produce ammonia and 1-aminocyclopropane-1-carboxylate while 4.65%, 12.28% and 27.91% produced Hydrogen cyanide, Indole acetic acid and solubilized phosphate respectively, making them important targets as biocontrol and biofertilizer agents. The growth of Fusarium graminearum was suppressed in vitro by 6.98% of the isolates. Plant growth promoting activities of rhizobacteria from bambara groundnut rhizosphere reveals that it has great potentials in food security as biofertilizer and biocontrol agent against fungal and bacterial pathogens.

scholarly journals Screening of Potential Plant Growth Promoting Properties of Bacillus Species Isolated from Different Regions of Nepal

2021 ◽  
Vol 9 (1) ◽  
pp. 79-84
Author(s):  
Enish Pathak ◽  
Arjun Sanjyal ◽  
Chhatra Raj Regmi ◽  
Saroj Paudel ◽  
Anima Shrestha
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Hydrogen Cyanide ◽  
Indole Acetic Acid ◽  
Agricultural Sector ◽  
Bacillus Species ◽  
Chemical Fertilizers ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

The deleterious effects of intensive use of chemical fertilizers and pesticides in agriculture has led to the substantial research efforts on finding the alternatives to these agrochemicals. This study was aimed to isolate Bacillus species from soil of different regions of Nepal and screen for their ability to promote plant growth directly or indirectly by testing their ability to produce plant growth hormone indole acetic acid, hydrogen cyanide, ammonia and protease as well as phosphate solubilization. Thirty nine Bacillus strains were isolated from 25 soil samples of different regions of Kathmandu and Chitwan districts of Nepal. These isolates were tested for plant growth promoting traits in vitro. Among the total isolates, about 48.7% were indole acetic acid producers, 38.4% of the isolates showed the ability to solubilize the phosphate, 71.8% were able to produce ammonia and all the isolates had the ability to produce hydrogen cyanide and protease. The isolated strains showed positive results to maximum PGPR traits and exhibited a potential to be used as alternatives to chemical fertilizers and pesticides and could be used as low-cost bio-based technology to promote plant growth in the agricultural sector.

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