The combined effect of arbuscular mycorrhizae and plant-growth-promoting yeast improves sunflower defense against Macrophomina phaseolina diseases

2019 ◽  
Vol 138 ◽  
pp. 104049 ◽  
Author(s):  
Nivien A. Nafady ◽  
Mohamed Hashem ◽  
Elhagag A. Hassan ◽  
Hoda A.M. Ahmed ◽  
Saad A. Alamri
Keyword(s):  
Plant Growth ◽  
Arbuscular Mycorrhizae ◽  
Macrophomina Phaseolina ◽  
Combined Effect ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant growth promoting endophyte Burkholderia contaminans NZ antagonizes phytopathogen Macrophomina phaseolina through melanin synthesis and pyrrolnitrin inhibition

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257863
Author(s):  
Nazia R. Zaman ◽  
Umar F. Chowdhury ◽  
Rifath N. Reza ◽  
Farhana T. Chowdhury ◽  
Mrinmoy Sarker ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Genome Mining ◽  
Gene Clusters ◽  
Macrophomina Phaseolina ◽  
Melanin Synthesis ◽  
Burkholderia Contaminans ◽  
Plant Growth Promoting ◽  
Growth Promoting

The endophytic bacterium Burkholderia contaminans NZ was isolated from jute, which is an important fiber-producing plant. This bacterium exhibits significant growth promotion activity in in vivo pot experiments, and like other plant growth-promoting (PGP) bacteria fixes nitrogen, produces indole acetic acid (IAA), siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. B. contaminans NZ is considered to exert a promising growth inhibitory effect on Macrophomina phaseolina, a phytopathogen responsible for infecting hundreds of crops worldwide. This study aimed to identify the possibility of B. contaminans NZ as a safe biocontrol agent and assess its effectiveness in suppressing phytopathogenic fungi, especially M. phaseolina. Co-culture of M. phaseolina with B. contaminans NZ on both solid and liquid media revealed appreciable growth suppression of M. phaseolina and its chromogenic aberration in liquid culture. Genome mining of B. contaminans NZ using NaPDoS and antiSMASH revealed gene clusters that displayed 100% similarity for cytotoxic and antifungal substances, such as pyrrolnitrin. GC-MS analysis of B. contaminans NZ culture extracts revealed various bioactive compounds, including catechol; 9,10-dihydro-12’-hydroxy-2’-methyl-5’-(phenylmethyl)- ergotaman 3’,6’,18-trione; 2,3-dihydro-3,5- dihydroxy-6-methyl-4H-pyran-4-one; 1-(1,6-Dioxooctadecyl)- pyrrolidine; 9-Octadecenamide; and 2- methoxy- phenol. These compounds reportedly exhibit tyrosinase inhibitory, antifungal, and antibiotic activities. Using a more targeted approach, an RP-HPLC purified fraction was analyzed by LC-MS, confirming the existence of pyrrolnitrin in the B. contaminans NZ extract. Secondary metabolites, such as catechol and ergotaman, have been predicted to inhibit melanin synthesis in M. phaseolina. Thus, B. contaminans NZ appears to inhibit phytopathogens by apparently impairing melanin synthesis and other potential biochemical pathways, exhibiting considerable fungistatic activity.

scholarly journals Biocontrol of Macrophomina phaseolina (Tassi) Goid causing charcoal rot disease in Lycopersicon esculentum L. by using multi species bacterial consortia

2021 ◽  
Vol 22 (3) ◽  
pp. 441-449
Author(s):  
Pradeep Kumar ◽  
Sandeep Kumar ◽  
R.C. Dubey
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Growth Promotion ◽  
Macrophomina Phaseolina ◽  
Growth And Yield ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting bacterial strains (LEP1-LEP31) were isolated from rhizosphere of Lycopersicon esculentum L. (Tomato) and screened for their plant growth promoting (PGP) activities. On the basis of morphological, physiological, biochemical, carbon source utilization and molecular characterization, these strains were identified as Pseudomonas sp., Azotobacter sp. and Bacillus sp. For antagonistic activities all the strains were subject to the chitinase activities by the development of clear halo around the inoculated bacterial spots when loaded on chitin (0.2%) supplemented mediumBased on pot and field trial results of individual strains and consortium application, it may be concluded that all the three strains i.e. Pseudomonas sp. LEP17, Azotobacter sp. strain LEP21 and Bacillus sp. strain LEP25 showed plant growth promoting effects. The growth promotion provided by these strains was apparently related to improve shoot and root development, which resulted in better nutrient uptake capability and suppression of plant pathogen. All these three strains were superior in this regard because they provided the best and most consistent effects on growth and yield of L. esculentum. All these strains Pseudomonas sp. LEP17, Azotobacter sp. strain LEP21, Bacillus sp. strain LEP25 and their consortium seems to be suitable for use as a plant growth promoting and improvement of growth and yield

Enhanced production of podophyllotoxins by co-culture of transformed Linum album cells with plant growth-promoting fungi

2010 ◽  
Vol 82 (1) ◽  
pp. 227-241 ◽  
Author(s):  
Ashish Baldi ◽  
Sunita Farkya ◽  
Abhishek Jain ◽  
Nishant Gupta ◽  
Rahul Mehra ◽  
...  
Keyword(s):  
Plant Growth ◽  
Arbuscular Mycorrhizae ◽  
Product Formation ◽  
Suspension Cultures ◽  
Promoting Effect ◽  
Linum Album ◽  
Enhanced Production ◽  
Culture Filtrates ◽  
Plant Growth Promoting ◽  
Growth Promoting

To investigate the plant growth-promoting effect and stimulation of lignan biosynthesis, the effect of culture filtrates/live co-culture of two arbuscular mycorrhizae-like fungi, Piriformospora indica and Sebacina vermifera, on growth of Linum album cells and on production of podophyllotoxin (PT) and 6-methoxypodophyllotoxin (6-MPT) was studied. For elicitation studies, different volumes of culture filtrates (autoclaved and membrane-filtered) of P. indica/S. vermifera were added to suspension cultures of L. album. The culture filtrates of both the fungi exhibited a positive effect on product formation. For co-culture experiments, both fungi were individually co-cultivated at different concentrations with L. album in suspension cultures for different time periods. This resulted in significant enhancement of PT and 6-MPT content in the plant cells. The activity of phenylalanine ammonia lyase (PAL) was observed to be related to the lignan accumulation, indicating its role as the key enzyme of the phenylpropanoid pathway. The study resulted in total lignan (PT and 6-MPT) production of 745.6 mg/l with a very high PT productivity of 52.4 mg/(l.d).

scholarly journals Effect of systemic fungicides on the efficacy of Pseudomonas aeruginosa (Schroeter) migula in the control of root-infecting fungi of wheat

2013 ◽  
Vol 53 (2) ◽  
pp. 39-46
Author(s):  
I. A. Siddiqui ◽  
S. S. Shaukat ◽  
S. Ehteshamul-Haque ◽  
S. A. Khan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Plant Growth ◽  
Grain Yield ◽  
Macrophomina Phaseolina ◽  
Effective Control ◽  
Bacterial Survival ◽  
Thiophanate Methyl ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Wheat Seeds

Effect of various fungicides on the efficacy of <i>Pseudomonas aeruginosa</i>, a plant growth-promoting rhizobacterium in the control of root-infecting fungi such as <i>Macrophomina phaseolina, Fusarium oxysporum</i> and <i>F.solani</i> on four different varieties of wheat was evaluated under field conditions. Bayleton (a.i. triadimephon), Bavistin (a.i. carbendazym) and Topsin-M (a.i. thiophanate-methyl) reduced bacterial survival on wheat seeds whereas Benlate (a.i. benomyl) was not effective in this respect. <i>P.aeruginosa</i> used in combination with Benlate showed effective control of soilborne root-infecting fungi along with the enhancement of growth and grain yield of wheat.

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