scholarly journals Isolation and molecular identification of laccase-producing saprophytic/phytopathogenic mushroom-forming fungi from various ecosystems in Michoacán State, Mexico

2019 ◽  
Vol 54 (1) ◽  
Author(s):  
Irum Mukhtar ◽  
Marina Arredondo-Santoyo ◽  
Ma. Soledad Vázquez-Garcidueñas ◽  
Gerardo Vázquez-Marrufo
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Soil Samples ◽  
The State ◽  
Soil Particle ◽  
Sequence Comparisons ◽  
Internal Transcribed Spacer Regions ◽  
Lab Experiments ◽  
Geographic Regions

The aim of this study was isolation and molecular identification of laccase-producing saprophytic/phytopathogen Basidiomycetes species from different geographic regions with dominant vegetation of <em>Pinus</em>, <em>Abies</em>, and <em>Quercus</em> spp. in the state of Michoacán, Mexico. Soil samples and visible mycelial aggregates were collected for fungal isolations. Soil samples were processed using a soil particle washing technique, where a selective Ascomycetes inhibitor and guaiacol, as an indicator of saprophytic Basidiomycetes growth, were used. Most of the isolates were obtained from samples collected in Parque Nacional, José Ma. Morelos (Km 23), Charo, Michoacán, Mexico. Based on sequence comparisons and phylogenetic analysis of internal transcribed spacer regions (ITS1-5.8S-ITS4) with respect to reference taxa, identification of saprophytic/phytopathogen Basidiomycetes species was carried out. In total, 15 isolates from 12 genera (i.e., <em>Bjerkandera</em>, <em>Coriolopsis</em>, <em>Ganoderma</em>, <em>Hexagonia</em>, <em>Irpex</em>, <em>Limonomyces</em>, <em>Psathyrella</em>, <em>Peniophora</em>, <em>Phlebia</em>, <em>Phlebiopsis</em>, <em>Trametes</em>, and <em>Trichaptum</em>) and one species from family Corticiaceae were identified. This study will be useful for further investigations on biodiversity of soil Basidiomycetes in different ecosystems. At present, these isolates are being used in our various lab experiments and can be useful in different industrial and bioremediation applications.

scholarly journals A new species of Acrophialophora from Guizhou Province, China

Phytotaxa ◽  
2017 ◽  
Vol 302 (3) ◽  
pp. 266
Author(s):  
YANWEI ZHANG ◽  
YAO WANG ◽  
GUI-PING ZENG ◽  
WANHAO CHEN ◽  
ZOU XIAO ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Distinct Species ◽  
Soil Samples ◽  
Guizhou Province ◽  
Beijing City ◽  
Living Culture ◽  
A New Species ◽  
Β Tubulin

Acrophialophora liboensis, a new fungus from soil samples in Libo County, Guizhou Province, China, is illustrated and described on the basis of morphological and molecular sequences data. Phylogenetic analysis based on internal transcribed spacer (ITS) and β-tubulin sequences demonstrated that A. liboensis is a distinct species closely related to A. cinerea and A. furcata. Morphologically, A. liboensis is characterized by solitary and lateral phialides tapering into thin necks and long chains of ellipsoidal or oval conidia. The ex-type living culture has been deposited in CGMCC, Beijing City, China.

Lithobieae and Eriocnemeae: two new Neotropical tribes of Melastomataceae

Phytotaxa ◽  
2020 ◽  
Vol 453 (3) ◽  
pp. 157-178
Author(s):  
DARIN S. PENNEYS ◽  
FRANK ALMEDA ◽  
FABIAN A. MICHELANGELI ◽  
RENATO GOLDENBERG ◽  
ANGELA B. MARTINS ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Southeastern Brazil ◽  
Monotypic Genus ◽  
Internal Transcribed Spacer Regions ◽  
New Tribe ◽  
Seed Data ◽  
Close Relatives

Several genera in the Melastomataceae mostly endemic to southeastern Brazil have not been assessed for their tribal placement in a phylogenetic context. Most workers have placed the monotypic diminutive herb Lithobium in the Microlicieae, but some classifications have assigned it to either the Sonerileae or Bertolonieae. Another monotypic genus, Eriocnema, has been included in the Bertolonieae, Sonerileae, or Microlicieae. Physeterostemon contains five species, and has not previously been assigned to any tribe. Ochthephilus, a monotypic genus endemic to Guyana, was assigned to the Merianieae, but lack of fruit and seed data, and a paucity of collections, have impeded phylogenetic assessment. To assess the placement of these genera, we conducted a phylogenetic analysis of 91 species representing 73 genera from across the Melastomataceae, including 24 of the 27 putative close relatives of Lithobium, Eriocnema, Ochthephilus, and Physeterostemon and seven gene regions, i.e., the nuclear ribosomal external and internal transcribed spacer regions, and the plastid regions accD-psaI, ndhF, psbK-psbL, rbcL, and rpl16. Lithobium groups as sister to a clade comprising all non-Olisbeoideae, Pternandreae, Henrietteeae, and Astronieae tribes. A new tribe, Lithobieae, is thus created to accommodate this placement. The capsular-fruited Eriocnema, Ochthephilus, and Physeterostemon form a clade that is sister to the berry-fruited Miconieae and widely separated from tribes with which they have been associated in historical classifications. Thus another new tribe, Eriocnemeae, is created. Lectotypes are designated for E. acaulis and L. cordatum.

scholarly journals A Novel Report on Killer Yeast Strains Identification Methods

2020 ◽  
Vol 33 (1) ◽  
pp. 39-49
Author(s):  
Najwa M.J.A. Abu-Mejdad ◽  
Adnan I. Al-Badran ◽  
Abdullah H. Al-Saadoon
Keyword(s):  
Soil Sample ◽  
Molecular Identification ◽  
Surface Sediment ◽  
Internal Transcribed Spacer ◽  
Soil Samples ◽  
Colour Reaction ◽  
Identification Methods ◽  
Yeast Strains ◽  
Killer Yeasts ◽  
Uncultivated Soil

This study was conducted to isolate and identify killer yeasts from soil samples that collected from different locations in Basrah and Dhi-Qar provinces. Seventy-five soil samples were collected from different areas, including sandy, arable, surface sediment and uncultivated soil, using dilution methods to cultivate a serial dilution of each soil sample. The results showed that a 112 isolates were identified biochemically using VITEK system and molecularly using internal transcribed spacer (ITS1- 5.8S-ITS2) marker. The molecular identification provided fast and precise identification results for the 112 isolates, whereas the VITEK test resulted low identification efficiency (8.2% were accurate and 91.8 % were not). The Diazonium blue B salts produced a good colour reaction in distinguishing between ascomycetes and basidiomycetes. The PCR was more accurate in identification of killer yeasts compared to the VITEK system.

scholarly journals Phylogenetic analysis ofPythium insidiosumThai strains using cytochrome oxidase II (COXII) DNA coding sequences and internal transcribed spacer regions (ITS)

2011 ◽  
Vol 49 (3) ◽  
pp. 289-295 ◽  
Author(s):  
Patcharee Kammarnjesadakul ◽  
Tanapat Palaga ◽  
Kallaya Sritunyalucksana ◽  
Leonel Mendoza ◽  
Theerapong Krajaejun ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Cytochrome Oxidase ◽  
Internal Transcribed Spacer ◽  
Coding Sequences ◽  
Dna Coding ◽  
Internal Transcribed Spacer Regions ◽  
Cytochrome Oxidase Ii

scholarly journals First confirmed report on Fusarium sporotrichioides on Pinus ponderosa var. jeffreyi in Slovakia

2016 ◽  
Vol 52 (No. 4) ◽  
pp. 250-253 ◽  
Author(s):  
Ivanová Helena ◽  
Hrehová Ľudmila ◽  
Pristaš Peter
Keyword(s):  
Phylogenetic Analysis ◽  
Pinus Ponderosa ◽  
Internal Transcribed Spacer ◽  
Potato Dextrose Agar ◽  
Its Sequences ◽  
Its Sequence ◽  
Fusarium Sporotrichioides ◽  
Sequence Comparisons ◽  
Red Pigments ◽  
Petri Dishes

During 2014–2015, samples from ten 20–25 years old pine (Pinus ponderosa var. jeffreyi) trees showing wilt symptoms were collected from the Arborétum Mlyňany park. A disease was observed on 20% of the trees. The first symptoms are wilting, stunting, chlorosis, and discolouration of needles, which turned yellow on affected twigs, then red and finally they fell off. Isolations of the pathogen were done from the discoloured tissues of needles (twenty samples from each tree) on Potato Dextrose Agar. Colonies of the fungus (3–4 Petri dishes from each tree) were initially aerial, white or slightly violet, but with age they became red and red pigments were produced in agar. The observed micromorphological characteristics of the fungus, such as presence of simple and proliferating conidiophores with polyphialides, microconidia, macroconidial shape, and chlamydospore presence matched the description of Fusarium sporotrichioides. The identity of the fungus was confirmed by phylogenetic analysis of internal transcribed spacer (ITS) sequences. Sequence comparisons placed the fungus to the species F. sporotrichioides with similarity of 99.6% at the ITS sequence level.

Sign in / Sign up

Export Citation Format

Share Document