scholarly journals Molecular Identification of Some Ghanaian Mushrooms Using Internal Transcribed Spacer Regions

2017 ◽  
Vol 06 (03) ◽  
Author(s):  
Theresa Appiah ◽  
Christian Agyare ◽  
Yinggang Luo
Keyword(s):  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Regions

scholarly journals Isolation and molecular identification of laccase-producing saprophytic/phytopathogenic mushroom-forming fungi from various ecosystems in Michoacán State, Mexico

2019 ◽  
Vol 54 (1) ◽  
Author(s):  
Irum Mukhtar ◽  
Marina Arredondo-Santoyo ◽  
Ma. Soledad Vázquez-Garcidueñas ◽  
Gerardo Vázquez-Marrufo
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Soil Samples ◽  
The State ◽  
Soil Particle ◽  
Sequence Comparisons ◽  
Internal Transcribed Spacer Regions ◽  
Lab Experiments ◽  
Geographic Regions

The aim of this study was isolation and molecular identification of laccase-producing saprophytic/phytopathogen Basidiomycetes species from different geographic regions with dominant vegetation of <em>Pinus</em>, <em>Abies</em>, and <em>Quercus</em> spp. in the state of Michoacán, Mexico. Soil samples and visible mycelial aggregates were collected for fungal isolations. Soil samples were processed using a soil particle washing technique, where a selective Ascomycetes inhibitor and guaiacol, as an indicator of saprophytic Basidiomycetes growth, were used. Most of the isolates were obtained from samples collected in Parque Nacional, José Ma. Morelos (Km 23), Charo, Michoacán, Mexico. Based on sequence comparisons and phylogenetic analysis of internal transcribed spacer regions (ITS1-5.8S-ITS4) with respect to reference taxa, identification of saprophytic/phytopathogen Basidiomycetes species was carried out. In total, 15 isolates from 12 genera (i.e., <em>Bjerkandera</em>, <em>Coriolopsis</em>, <em>Ganoderma</em>, <em>Hexagonia</em>, <em>Irpex</em>, <em>Limonomyces</em>, <em>Psathyrella</em>, <em>Peniophora</em>, <em>Phlebia</em>, <em>Phlebiopsis</em>, <em>Trametes</em>, and <em>Trichaptum</em>) and one species from family Corticiaceae were identified. This study will be useful for further investigations on biodiversity of soil Basidiomycetes in different ecosystems. At present, these isolates are being used in our various lab experiments and can be useful in different industrial and bioremediation applications.

scholarly journals Identification of Medically Important Yeast Species by Sequence Analysis of the Internal Transcribed Spacer Regions

2007 ◽  
Vol 45 (3) ◽  
pp. 1079-1079
Author(s):  
S. N. Leaw ◽  
H. C. Chang ◽  
H. F. Sun ◽  
R. Barton ◽  
J.-P. Bouchara ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Yeast Species ◽  
Internal Transcribed Spacer Regions

scholarly journals WildTermitomycesSpecies Collected from Ondo and Ekiti States Are More Related to African Species as Revealed by ITS Region of rDNA

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Victor Olusegun Oyetayo
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Its Sequence ◽  
African Countries ◽  
African Species ◽  
Its Sequence Analysis ◽  
Degree Of Similarity

Molecular identification of eighteenTermitomycesspecies collected from two states, Ondo and Ekiti in Nigeria was carried out using the internal transcribed spacer (ITS) region. The amplicons obtained from rDNA ofTermitomycesspecies were compared with existing sequences in the NCBI GenBank. The results of the ITS sequence analysis discriminated between all theTermitomycesspecies (obtained from Ondo and Ekiti States) andTermitomycessp. sequences obtained from NCBI GenBank. The degree of similarity of T1 to T18 to gene ofTermitomycessp. obtained from NCBI ranges between 82 and 99 percent.Termitomycesspecies from Garbon with ascension number AF321374 was the closest relative of T1 to T18 except T12 that has T. eurhizus and T. striatus as the closet relative. Phylogenetic tree generated with ITS sequences obtained from NCBI GenBank data revealed that T1 to T18 are more related toTermitomycesspecies indigenous to African countries such as Senegal, Congo, and Gabon.

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