Influence of lead on the chlorophyll content and on initial steps of its synthesis in greening cucumber seedlings

Lead uptake by young cucumber (Couches satires L.) seedlings growing in 10-4 and 10-3 M PbCl2 solution caused δ-aminolevulinic acid synthesis inhibition, reduced the activity of δ-aminolevulinic acid dehydratase and the chlorophyll centent in the cotyledons. Lead mainly accumulated in the roots (ca 93-96% of Pb uptake) and hypocotyls (ca 4-6%), whereas only trace Pb amounts were found in the cotyledons when 10-3 M solution was used. It is supposed that one of the causes of the reduction of chlorophyll synthesis by lead is its influence on tissue hydration which diminished to about 50 per cent as compared with the control. The direct effect of lead on the examined steps of chlorophyll synthesis cannot, however, be ruled out.

Download Full-text

Chilling-induced chlorosis in maize (Zea mays)

Canadian Journal of Botany ◽

10.1139/b85-089 ◽

1985 ◽

Vol 63 (4) ◽

pp. 711-715 ◽

Cited By ~ 4

Author(s):

R. Hodgins ◽

R. B. van Huystee

Keyword(s):

Zea Mays ◽

Chlorophyll Content ◽

Sweet Corn ◽

Aminolevulinic Acid ◽

Chlorophyll Synthesis ◽

Carotenoid Content ◽

Control Tissue ◽

Etiolated Seedlings ◽

Chilling Temperatures ◽

And Control

The effect of chilling temperatures on the porphyrin pathway leading to chlorophyll was studied in Seneca Chief hybrid sweet corn. One-week-old seedlings grown at 28 °C in a 14 h light: 10 h dark photoperiod synthesize negligible amounts of chlorophyll when exposed to 12 °C for a subsequent 6 d. When the chilled plant is then brought back to 28 °C, chlorophyll synthesis is restored to control levels. Little difference in carotenoid content was detected between chill-stressed and control tissue even after 4 d of stress. Small differences in the chlorophyll content per 106 chloroplasts could be detected between stressed and control seedlings. Etiolated seedlings synthesize negligible amounts of chlorophyll or its precursors when illuminated at 12 °C. Incubation of tissue with aminolevulinic acid at various temperatures from 12 to 22 °C resulted in an accumulation of precursors comparable to 28 °C control tissue. The ability of etiolated tissue to accumulate aminolevulinic acid was negligible when illuminated at 12 °C as compared with that in tissue illuminated at 28 °C.

Download Full-text

Inhibition of chlorophyll synthesis in Hordeum vulgare by 3-amino 2,3-dihydrobenzoic acid (gabaculin)

Bioscience Reports ◽

10.1007/bf01119876 ◽

1985 ◽

Vol 5 (9) ◽

pp. 775-781 ◽

Cited By ~ 41

Author(s):

Corinne M. Hill ◽

Sharon A. Pearson ◽

Arnold J. Smith ◽

Lyndon J. Rogers

Keyword(s):

Hordeum Vulgare ◽

Potent Inhibitor ◽

Acid Synthesis ◽

Chlorophyll Synthesis ◽

Dual Inhibitor ◽

Aminolaevulinic Acid ◽

Acid Dehydratase ◽

Leaf Segments ◽

Chlorophyll Formation ◽

Aminolaevulinic Acid Dehydratase

Gabaculin (3-amino 2,3-dihydrobenzoic acid) is shown to be a very potent inhibitor of chlorophyll formation in Hordeum vulgate. Exposure of leaf segments to 30/μM gabaculin results in an 80% inhibition of chlorophyll synthesis, and this is paralleled by a decrease in carotenoid. Dual-inhibitor studies with dioxoheptanoic acid, which is an inhibitor of δ-aminolaevulinic acid dehydratase, show that gabaculin inhibits an earlier step than dioxoheptanoic acid and affects δ-aminolaevulinic acid synthesis rather than its subsequent metabolism.

Download Full-text

Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis

Comparative Biochemistry and Physiology Part B Comparative Biochemistry ◽

10.1016/0305-0491(88)90143-5 ◽

1988 ◽

Vol 91 (2) ◽

pp. 279-284 ◽

Cited By ~ 1

Author(s):

Maria Elisa Lombardo ◽

Lidia Susana Araujo ◽

Adela Ana Juknat ◽

Alcira M. Del C. Batllet

Keyword(s):

Chlorophyll Content ◽

Euglena Gracilis ◽

Aminolevulinic Acid ◽

Acid Synthesis

Download Full-text

5-Aminolevulinic Acid (ALA) Alleviated Salinity Stress in Cucumber Seedlings by Enhancing Chlorophyll Synthesis Pathway

Frontiers in Plant Science ◽

10.3389/fpls.2018.00635 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 29

Author(s):

Yue Wu ◽

Xin Jin ◽

Weibiao Liao ◽

Linli Hu ◽

Mohammed M. Dawuda ◽

...

Keyword(s):

Salinity Stress ◽

Aminolevulinic Acid ◽

Chlorophyll Synthesis ◽

Cucumber Seedlings

Download Full-text

Photosynthetic pigments content, δ-aminolevulinic acid dehydratase and acid phosphatase activities and mineral nutrients concentration in cadmium-exposed Cucumis sativus L.

Biologia ◽

10.2478/s11756-009-0034-6 ◽

2009 ◽

Vol 64 (2) ◽

Cited By ~ 16

Author(s):

Jamile Gonçalves ◽

Fernando Nicoloso ◽

Alexssandro Becker ◽

Luciane Pereira ◽

Luciane Tabaldi ◽

...

Keyword(s):

Acid Phosphatase ◽

Cucumis Sativus ◽

Photosynthetic Pigments ◽

Aminolevulinic Acid ◽

Mineral Nutrients ◽

Root Tip ◽

Cucumis Sativus L ◽

Phosphatase Activities ◽

Cucumber Seedlings ◽

Acid Dehydratase

AbstractIn this study, the effects of cadmium chloride (CdCl2) on plant growth, histology of roots, photosynthetic pigments content, δ-aminolevulinic acid dehydratase (ALA-D; E.C. 4.2.1.24) and acid phosphatase activities (AP; E.C. 3.1.3.2), soluble phosphorus (Pi) measurement and mineral nutrients content in cucumber seedlings (Cucumis sativus L.) were investigated. Cucumber seedlings were grown in vitro in an agar-solidified substrate containing four CdCl2 treatments (0, 100, 400, and 1000 μM) for ten days. Cd was readily absorbed by seedlings and its content was greater in the roots than in the shoot. Cd reduced shoot and root length, and fresh and dry biomass of seedlings. Inhibition of root cell elongation in Cd-treated seedlings was observed by the increase of the mean radial size of cells belonging to three zones of the root tip. The highest level of Cd reduced in a similar manner chlorophyll a, chlorophyll b and total chlorophyll contents. Increasing concentrations of Cd resulted in a linear decrease in carotenoids levels of cotyledons. Interestingly, the ALA-D activity in cotyledons was inhibited only at the highest level of Cd. Root and shoot AP activities were, respectively, activated and inhibited at all CdCl2 concentrations. Root Pi concentration was increased in all Cd treatments and it was not altered in the shoot tissues. Moreover, in general, the nutrient contents were increased in the root and decreased in the shoot. Therefore, we suggest that Cd affects negatively growth, photosynthetic pigments, ALA-D and AP activities and partition of mineral nutrients in cucumber seedlings.

Download Full-text