scholarly journals The effect of benzene and its Chlorella vulgaris metabolites on the growth of tobacco tissue

2014 ◽  
Vol 64 (4) ◽  
pp. 366-370
Author(s):  
E. Szczepkowska-Kuźnicka ◽  
W. Wardas
Keyword(s):  
Nicotiana Tabacum ◽  
Toxic Effect ◽  
Chlorella Vulgaris ◽  
Microscopic Examination ◽  
Callus Tissue ◽  
Tissue Growth ◽  
Tobacco Callus ◽  
Cultivation Medium

The influence of benzene, phenol and catechol on the tobacco callus tissue (<i>Nicotiana tabacum</i> L. cv. Wisconsin No. 38) cultivated <i>in vitro</i> was studied. Although inhibition of callus tissue growth in relation to the control was observed in all variants, the benzene-induced inhibition was the most significant. When catechol and phenol were added to the cultivation medium together, callus tissue growth was similar to that of the control. The addition of catechol to the medium containing phenol presumably reduces the toxic effect of phenol on the callus tissue examined. Callus tissue growing in the presence of phenol, benzene and cathechol became senescent more rapidly than in the control. Moreover microscopic examination revealed significant changes in the callus structure.

scholarly journals Physiological and biochemical effects of morphactin IT 3233 on callus and tumour tissues of Nicotiana tabacum L. cultured in vitro. I. Growth and nitrogen compounds content

2015 ◽  
Vol 43 (1) ◽  
pp. 59-70
Author(s):  
Z. Chirek
Keyword(s):  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Dry Mass ◽  
Tissue Growth ◽  
Nitrogen Compounds ◽  
Nitrogen Pool ◽  
Biochemical Effects ◽  
Nicotiana Tabacum L ◽  
Physiological And Biochemical

A significant limitation of callus tissue growth was noted in <i>Nicotiana tabacum</i> L. cultured in vitro under the influence of morphactin IT 3233 applied in 10 - 40 mg/dm<sup>3</sup> concentrations. Growth inhibition was associated with an increase in dry mass content and the contribution of protein N to the nitrogen, pool. Tumour tissue of tobacco under the same conditions reacted weaker to morphactin. Growth limitation reached 25 per cent as compared with the control, dry mass and nitrogen compounds content showed only slight variations. It is supposed that the different sensitivity of both types of tissues to morphactin is connected with their different hormonal metabolism.

scholarly journals Physiological and biochemical effects of morphactin IT 3233 on callus and tumour tissues of Nicotiana tabacum L. cultured in vitro. II. Protein synthesis and respiratory activity

2015 ◽  
Vol 43 (1) ◽  
pp. 71-80
Author(s):  
Z. Chirek
Keyword(s):  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Respiratory Activity ◽  
Tissue Growth ◽  
The Other ◽  
Biochemical Effects ◽  
Nicotiana Tabacum L ◽  
Callus Tissues ◽  
Physiological And Biochemical

It was found that the inhibition of callus tissue growth in <i>Nicotiana tabacum</i> L. cultured <i>in vitro</i> by the application of morphactin IT 3233 is associated with a rise of the protein level in spite of a 50 per cent depression of its synthesis. Respiratory activity of these tissues is also lowered. Tumour tissues, on the other hand, show only light changes in the parameters studied. It would seem that morphactin depresses a metabolic activity in the callus tissues, and probably causes deposition of a, large quantity of metabolically inactive proteins.

Growth Inhibition in Tobacco (Nicotiana tabacum) Callus by 2,6-Dinitroaniline Herbicides and Protection by D-α-tocopherol Acetate

Weed Science ◽  
1978 ◽  
Vol 26 (6) ◽  
pp. 527-530 ◽  
Author(s):  
J. B. Huffman ◽  
N. D. Camper
Keyword(s):  
Regression Analysis ◽  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Linear Regression Analysis ◽  
Tissue Growth ◽  
Tissue Cultures ◽  
Fresh Weight ◽  
Structural Variations ◽  
Tocopherol Acetate ◽  
Dinitroaniline Herbicides

Tobacco(Nicotiana tabacumL. ‘X-73’) callus tissue cultures were used in a bioassay system for determining the effect of the following substituted 2,6-dinitroaniline herbicides on growth: trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine); oryzalin (3,5-dinitro-N4,N4-dipropylsulfanilamide); benefin(N-butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-toluidine); AC 92390(N-sec-butyl-2,6-dinitro-3,4-xylidine); penoxalin [N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine]; GS-38946(N-ethyl-N-tetrahydrofurfuryl-4-trifluoromethyl-2,6-dinitroaniline); chlornidine [N,N-di(2-chloroethyl)-4-methyl-2,6-dinitroaniline]; nitralin [4-(methylsulfonyl)2,6-dinitro-N,N-dipropylaniline]; dinitramine(N4,N4-diethyl-α,α,α-trifluoro-3,5-dinitrotoluene-2,4-diamine); isopropalin (2,6-dinitro-N,N-dipropylcumidine), and profluralin [N(cyclopropylmethyl)-α,α,α-trifluoro-2,6-dinitro-N-propyl-p-toluidine]. The molar concentration required to inhibit fresh weight gain by 50% (I50) was determined by using linear regression analysis on data from a range of five concentrations of each chemical. I50values did not correlate with structural variations or available physical constants. Herbicides listed in order of increasing activity are AC 92390< GD-38946<profluralin = isopropalin<benefin = chlornidine = trifluralin = nitralin<oryzalin = penoxalin < dinitramine. Exogenously applied D-α-tocopherol acetate at 100 times the I50concentrations decreased the inhibition of tissue growth by the herbicides.

scholarly journals Physiological and biochemical effects of morphactin IT 3233 on callus and tumour tissues of Nicotiana tabacum L. cultured in vitro. IV. IAA oxidase activity. Oat coleoptile biotest

2015 ◽  
Vol 43 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Z. Chirek
Keyword(s):  
Oxidase Activity ◽  
Callus Tissue ◽  
Tissue Growth ◽  
Tumour Tissue ◽  
Iaa Oxidase ◽  
Nicotiana Tabacum L ◽  
Coleoptile Elongation ◽  
Short Time ◽  
Physiological And Biochemical

IAA oxidase activity in callus and tumour tissue of tobacco subjected to the action of morphactin IT 3233 for shorter and longer periods was determined. Control tumour tissue shows an activity higher by about 40 per cent as compared with that of callus tissue. Morphactin applied for a short time (24-h incubation) does not change the activity of the enzyme. When application is prolonged, a considerable enhancement (up to 140%) of the enzyme activity in callus tissue is observed in dependence on the morphactin concentration. In tumour tissues the activity is stimulated by 45 per cent as compared to control. Oat coleoptile elongation growth induced by IAA is limited to 40 per cent when morphactin is added in the concentrations used for tobacco tissue cultures. The possibility of the morphactin action on tissue growth via IAA metabolism is discussed.

scholarly journals Limitation of Tobacco Callus Tissue Growth by Carbohydrate Availability

1970 ◽  
Vol 46 (1) ◽  
pp. 118-122 ◽  
Author(s):  
C. D. Upper ◽  
John P. Helgeson ◽  
G. T. Haberlach
Keyword(s):  
Callus Tissue ◽  
Tissue Growth ◽  
Tobacco Callus ◽  
Carbohydrate Availability

scholarly journals Influence of hydroxyurea on nucleic acids content and 3H-uridine incorporation in callus and tumorous tobacco tissues cultured in vitro

2015 ◽  
Vol 44 (3) ◽  
pp. 393-405
Author(s):  
A. Bielecka
Keyword(s):  
Nicotiana Tabacum ◽  
Nucleic Acids ◽  
Dna Content ◽  
Incubation Medium ◽  
Rna Synthesis ◽  
Callus Tissue ◽  
Tumour Tissue ◽  
Inhibitory Influence ◽  
Tumor Tissues

In callus and tumor tissues of <i>Nicotiana tabacum</i> cultured for 39 days in media supplemented with various concentrations of hydroxyurea (1.3 x 10<sup>-4</sup> M - 1.3 x 10<sup>-3</sup> M) a decrease of DNA content (ca. 24 per cent in callus tissue and ca. 23 per cent in tumour tissue) and a decrease of RNA content (over 10 per cent and ca. 9 per cent in callus and tumour tissue, respectively) was observed. The autoradiographic method showed that a long-lasting action of this com-pound inhibits RNA synthesis. A stronger inhibitory influence of hydroxyurea upon incorporation of 3H-uridine from the incubation medium was revealed.

scholarly journals Improvement of growth parameters of prune callus cultures destined to initiate celi suspensions

2011 ◽  
Vol 75 (1) ◽  
pp. 5-9
Author(s):  
Ewa Hanus-Fajerska
Keyword(s):  
Small Proportion ◽  
Callus Tissue ◽  
Growth Parameters ◽  
Leaf Explants ◽  
Callus Cultures ◽  
Tissue Growth ◽  
Proliferative Capacity ◽  
Prunus Domestica ◽  
Murashige And Skoog Medium

Callus was inducted on wounded leaf explants from shoot tips of a particular <em>Prunus domestica</em> 'Węgierka Zwykła' clone cultivated in vitro. The improvement of Sweet Common Prune stock callus tissue parameters has been approached by experiments on culture protocols. Either for the induction or maintenance of tissue modified Murashige and Skoog medium, supplemented with different auxins and cytokinins at varying concentrations, was used. The goal was to obtain the highiest possible proliferative capacity of friable tissue without any signs of cell redifferentiation for about 10 weeks. The choice of auxin was an important factor regulating the rate and kind of tissue growth, and for the examined prune clone auxin alone brought a relatively small proportion of cells into division, so advantageous was to combine it with oxygenated cytokinin. Friable tissue was obtained on media supplemented with dicamba or with picloram, but not with 2.4-D neither alone nor combinated with IBA.

scholarly journals Influence of icariin on inflammation, apoptosis, invasion, and tumor immunity in cervical cancer by reducing the TLR4/MyD88/NF-κB and Wnt/β-catenin pathways

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Chunyang Li ◽  
Shuangqing Yang ◽  
Huaqing Ma ◽  
Mengjia Ruan ◽  
Luyan Fang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Underlying Mechanism ◽  
Tissue Growth ◽  
Migration And Invasion ◽  
Dependent Manner ◽  
Antitumor Effects ◽  
Siha Cells ◽  
Cervical Cancer Cells

Abstract Background Cervical cancer is a type of the most common gynecology tumor in women of the whole world. Accumulating data have shown that icariin (ICA), a natural compound, has anti-cancer activity in different cancers, including cervical cancer. The study aimed to reveal the antitumor effects and the possible underlying mechanism of ICA in U14 tumor-bearing mice and SiHa cells. Methods The antitumor effects of ICA were investigated in vivo and in vitro. The expression of TLR4/MyD88/NF-κB and Wnt/β-catenin signaling pathways were evaluated. Results We found that ICA significantly suppressed tumor tissue growth and SiHa cells viability in a dose-dependent manner. Also, ICA enhanced the anti-tumor humoral immunity in vivo. Moreover, ICA significantly improved the composition of the microbiota in mice models. Additionally, the results clarified that ICA significantly inhibited the migration, invasion capacity, and expression levels of TGF-β1, TNF-α, IL-6, IL-17A, IL-10 in SiHa cells. Meanwhile, ICA was revealed to promote the apoptosis of cervical cancer cells by down-regulating Ki67, survivin, Bcl-2, c-Myc, and up-regulating P16, P53, Bax levels in vivo and in vitro. For the part of mechanism exploration, we showed that ICA inhibits the inflammation, proliferation, migration, and invasion, as well as promotes apoptosis and immunity in cervical cancer through impairment of TLR4/MyD88/NF-κB and Wnt/β-catenin pathways. Conclusions Taken together, ICA could be a potential supplementary agent for cervical cancer treatment.

Sign in / Sign up

Export Citation Format

Share Document