scholarly journals The effects of diversified phosphorus nutrition on the growth of oat (Avena sativa L.) and acid phosphatase activity

2018 ◽  
Vol 87 (1) ◽  
Author(s):  
Ewa Żebrowska ◽  
Kamila Zujko ◽  
Anna Kuleszewicz ◽  
Iwona Ciereszko
Keyword(s):  
Acid Phosphatase ◽  
Phytic Acid ◽  
Root Exudates ◽  
Avena Sativa ◽  
Growth Parameters ◽  
Root Culture ◽  
Nutrient Media ◽  
Split Root ◽  
Split Root System ◽  
Apase Activity

We studied the effect of differential phosphorus (P) supply on the development of oat seedlings (<em>Avena sativa</em> L. ‘Arab’) as well as localization and activity of acid phosphatases in tissues and root exudates. Plants were grown for 1–3 weeks on nutrient media with inorganic phosphate (+P, control), reduced Pi (0.1 P), phytic acid (PA) as organic P source, and without P addition (−P), in standard conditions or in a split-root culture system. Phosphate starvation reduced shoot growth but increased root elongation and root/shoot ratio, whereas 0.1 P and PA oat plants had similar growth parameters to +P plants. The growth on −P medium significantly decreased Pi content in all tissues, but only a slight Pi decrease was observed in plants grown on 0.1 P and PA media or various split-root system conditions. Pi starvation led to an increase in acid phosphatase (APase) activity in root exudates when compared to +P, 0.1 P, and PA plant samples. APase activity was especially intensive in root cross sections in rhizodermis and around/in vascular tissues of −P plants. For plants grown on 0.1 P medium and on phytic acid, APase activity did not change when compared to the control. Three major isoforms of APases were detected in plant tissues (similar in all studied conditions, with a higher activity of one isoform under Pi deficit). Generally, lowered Pi content (0.1 P) was not stressful to oat plants for up to 3 weeks of culture. Oat plants grew equally well on nutrient media with Pi and on media with phytate, although phytate was considered not available for other plants. The oat plants activated mainly extracellular APases, but also intracellular enzymes, rather via nonlocal signals, to acquire Pi from external/internal sources under Pi deficiency.

scholarly journals Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Iñigo Saiz-Fernández ◽  
Martin Černý ◽  
Jan Skalák ◽  
Břetislav Brzobohatý
Keyword(s):  
Arabidopsis Thaliana ◽  
Root System ◽  
Growth Parameters ◽  
Healing Process ◽  
Root Systems ◽  
Water Soluble ◽  
Split Root ◽  
Split Root System ◽  
Leaf Proteome

Abstract Background Split-root systems (SRS) have many applications in plant sciences, but their implementation, depending on the experimental design, can be difficult and time-consuming. Additionally, the system is not exempt from limitations, since the time required for the establishment of the SRS imposes a limit to how early in plant development experiments can be performed. Here, we optimized and explained in detail a method for establishing a SRS in young Arabidopsis thaliana seedlings, both in vitro and in soil. Results We found that the partial de-rooting minimized the recovery time compared to total de-rooting, thus allowing the establishment of the split-root system in younger plants. Analysis of changes in the Arabidopsis leaf proteome following the de-rooting procedure highlighted the distinct metabolic alterations that totally and partially de-rooted plants undergo during the healing process. This system was also validated for its use in drought experiments, as it offers a way to apply water-soluble compounds to plants subjected to drought stress. By growing plants in a split-root system with both halves being water-deprived, it is possible to apply the required compound to one half of the root system, which can be cut from the main plant once the compound has been absorbed, thus minimizing rehydration and maintaining drought conditions. Conclusions Partial de-rooting is the suggested method for obtaining split-root systems in small plants like Arabidopsis thaliana, as growth parameters, survival rate, and proteomic analysis suggest that is a less stressful procedure than total de-rooting, leading to a final rosette area much closer to that of uncut plants. Additionally, we provide evidence that split root-systems can be used in drought experiments where water-soluble compounds are applied with minimal effects of rehydration.

Reduced root secretion of valine in Rosa-microbe interaction contributes to the decreased colonization of pathogenic Agrobacterium tumefaciens

Plant Disease ◽  
2020 ◽  
Author(s):  
LIN CHEN ◽  
Qinghua Ma ◽  
Huihui Liu ◽  
Lusen Bian ◽  
XINGHONG WANG ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Root System ◽  
Disease Severity ◽  
Root Exudates ◽  
Crown Gall ◽  
Crown Gall Disease ◽  
Positive Effects ◽  
Split Root ◽  
Split Root System ◽  
The Rose

Root exudates play a critical role in root-microbe interactions. Agrobacterium tumefaciens causes crown gall disease in multiple plant species, but the rose root exudates-mediated inhibition of Agrobacterium in the rhizosphere is poorly understood. In this study, the influence of preinoculation with beneficial bacteria or pathogens on root exudates and subsequent colonization by A. tumefaciens was investigated in a split-root system. We found that preinoculation of rose plants in a split-root system with Bacillus velezensis CLA178 or A. tumefaciens C58 inhibited the subsequent colonization by C58. The root secretion of valine had positive effects on the chemotaxis, biofilm formation, colonization of C58 and crown gall disease severity, but the secretion of valine decreased significantly when Rosa multiflora plants were preinoculated with CLA178 or C58. These results indicated that the rose plants reduced the root secretion of valine in response to microbial colonization, thereby reducing the colonization of Agrobacterium and disease severity. This study provides new insights into the root exudates-mediated interactions of rose plants, B. velezensis and A. tumefaciens, and proposes a potential way for controlling crown gall disease.

scholarly journals Root Exudates of Stressed Plants Stimulate and Attract Trichoderma Soil Fungi

2018 ◽  
Vol 31 (10) ◽  
pp. 982-994 ◽  
Author(s):  
Nadia Lombardi ◽  
Stefania Vitale ◽  
David Turrà ◽  
Massimo Reverberi ◽  
Corrado Fanelli ◽  
...  
Keyword(s):  
Root Exudates ◽  
Stress Factors ◽  
Bioactive Molecules ◽  
Biotic And Abiotic Stress ◽  
Abiotic Stress Factors ◽  
Split Root ◽  
Tomato Roots ◽  
Split Root System ◽  
Response To Stress ◽  
Biocontrol Strain

Plant roots release complex mixtures of bioactive molecules, including compounds that affect the activity and modify the composition of the rhizosphere microbiome. In this work, we investigated the initial phase of the interaction between tomato and an effective biocontrol strain of Trichoderma harzianum (T22). We found that root exudates (RE), obtained from plants grown in a split-root system and exposed to various biotic and abiotic stress factors (wounding, salt, pathogen attack), were able to stimulate the growth and act as chemoattractants of the biocontrol fungus. On the other hand, some of the treatments did not result in an enhanced chemotropism on Fusarium oxysporum f. sp. lycopersici, indicating a mechanism that may be selective for nonpathogenic microbes. The involvement of peroxidases and oxylipins, both known to be released by roots in response to stress, was demonstrated by using RE fractions containing these molecules or their commercial purified analogs, testing the effect of an inhibitor, and characterizing the complex pattern of these metabolites released by tomato roots both locally and systemically.

Competitive attributes ofA. sativa, T. aestivum, andH. vulgareare conserved in no-till cropping systems

Weed Science ◽  
1999 ◽  
Vol 47 (6) ◽  
pp. 712-719 ◽  
Author(s):  
Anne Légère ◽  
Yuguang Bai
Keyword(s):  
Hordeum Vulgare ◽  
Avena Sativa ◽  
Growth Parameters ◽  
Cropping Systems ◽  
Seedling Emergence ◽  
Biomass Accumulation ◽  
Tillage Systems ◽  
Area Index ◽  
No Till ◽  
Lower Test

The robustness of competitive attributes of cereals such as rapid and uniform seedling emergence, tillering, early biomass accumulation and canopy closure, and height advantage over weeds have not yet been tested under environmental conditions typical of no-till (NT) cropping systems. Our objective was to evaluate the effects or NT practices on growth and productivity ofAvena sativa, Triticum aestivum, Hordeum vulgare, and associated weeds. The experiment was conducted on a Kamouraska clay at La Pocatière, QC, in 1994, 1995, and 1996.Avena sativa, T. aestivum, andH. vulgarewere grown under tilled and NT practices. Cereal growth parameters were measured six (1994) or seven (1995) times between planting and the 11th week after planting but only once in 1996. Grain yields and yield components were determined at crop maturity.Avena sativaandH. vulgarepopulations were little affected by tillage, whereasT. aestivumpopulations were reduced by 16 to 20% in NT systems. Growth in height in NT systems was either similar or greater than in tilled systems in all three cereals. Cereal leaf area index (LAI) and biomass accumulation was also comparable between tillage systems, except forT. aestivumLAI in 1994, which was greater in tilled plots on two sampling dates. Response of annual dicots to tillage was inconsistent in all crops. Annual monocots dominated in some but not all NT systems. Perennial dicots dominated in NT systems, whereas perennial monocots were more abundant in tilled systems in all three cereals.Avena sativaandT. aestivumyields in NT plots were comparable or greater than in tilled plots, in spite of having either lower test weights (A. sativa) or lower 1,000-grain weights (T. aestivum). NTT. aestivumproductivity was maintained in spite of reduced plant establishment.Hordeum vulgareyields were also similar across tillage systems, except in 1995, when yields in tilled plots were greater than in NT plots. The height advantage observed for NTH. vulgaredid not result in improved yields. All three cereals, and particularlyA. sativa, appeared well suited to NT systems, despite the pressure provided by different weed groups, compared to tilled systems. However, results suggest that NT production of cereals could benefit from improved attention to perennial dicot control and crop seedling establishment, particularly forT. aestivum.

Responses of soybean to ammonium and nitrate supplied in combination to the whole root system or separately in a split-root system

1994 ◽  
Vol 90 (2) ◽  
pp. 259-268 ◽  
Author(s):  
Sylvain Chaillou ◽  
James W. Rideout ◽  
C. David Raper, ◽  
Jean-Francois Morot-Gaudry
Keyword(s):  
Root System ◽  
Split Root ◽  
Split Root System

Effect of aluminum on soybean nodulation and nodule activity in a vertical split‐root system

1997 ◽  
Vol 20 (7-8) ◽  
pp. 963-974 ◽  
Author(s):  
D. M. Silva ◽  
L. Sodek
Keyword(s):  
Root System ◽  
Split Root ◽  
Split Root System ◽  
Soybean Nodulation

Comparison of the responses to NaCl stress of two pea cultivars using split-root system

2009 ◽  
Vol 123 (2) ◽  
pp. 164-169 ◽  
Author(s):  
Houneida Attia ◽  
Sarra Nouaili ◽  
Abdelaziz Soltani ◽  
Mokhtar Lachaâl
Keyword(s):  
Root System ◽  
Nacl Stress ◽  
Split Root ◽  
Split Root System

Biomass allocation in tomato (Lycopersicon esculentum) plants grown under partial rootzone drying: enhancement of root growth

2004 ◽  
Vol 31 (10) ◽  
pp. 971 ◽  
Author(s):  
Darren M. Mingo ◽  
Julian C. Theobald ◽  
Mark A. Bacon ◽  
William J. Davies ◽  
Ian C. Dodd
Keyword(s):  
Lycopersicon Esculentum ◽  
Root System ◽  
Biomass Allocation ◽  
Root Biomass ◽  
Leaf Water ◽  
Total Biomass ◽  
Split Root ◽  
Split Root System ◽  
Partial Rootzone Drying ◽  
And Control

Tomato (Lycopersicon esculentum Mill.) plants were grown in either a glasshouse (GH) or a controlled environment cabinet (CEC) to assess the effects of partial rootzone drying (PRD) on biomass allocation. Control and PRD plants received the same amounts of water. In control plants, water was equally distributed between two compartments of a split-root system. In PRD plants, only one compartment was watered while the other was allowed to dry. At the end of each drying cycle, wet and dry compartments were alternated. In the GH, total biomass did not differ between PRD and control plants after four cycles of PRD, but PRD increased root biomass by 55% as resources were partitioned away from shoot organs. In the CEC, leaf water potential did not differ between treatments at the end of either of two cycles of PRD, but stomatal conductance of PRD plants was 20% less at the end of the first cycle than at the beginning. After two cycles of PRD in the CEC, biomass did not differ between PRD and control plants, but PRD increased root biomass by 19% over the control plants. The promotion of root biomass in PRD plants was associated with the alternation of wet and dry compartments, with increased root biomass occurring in the re-watered compartment after previous exposure to soil drying. Promotion of root biomass in field-grown PRD plants may allow the root system to access resources (water and nutrients) that would otherwise be unavailable to control plants. This may contribute to the ability of PRD plants to maintain similar leaf water potentials to conventionally irrigated plants, even when smaller irrigation volumes are supplied.

scholarly journals Lowering of phytic acid content by enhancement of phytase and acid phosphatase activities during sunflower germination

2010 ◽  
Vol 53 (4) ◽  
pp. 975-980 ◽  
Author(s):  
Juliana da Silva Agostini ◽  
Rosicler Balduíno Nogueira ◽  
Elza Iouko Ida
Keyword(s):  
Acid Phosphatase ◽  
Phytic Acid ◽  
Acid Content ◽  
Nutritional Value ◽  
Phytase Activity ◽  
Phytic Acid Content ◽  
Phosphatase Activities ◽  
Hybrid Sunflower

The objective of this work was to investigate the germination of hybrid sunflowers BRS191 and C11 as a means of lowering phytic acid (PA) content by enhancing the activity of endogenous phytase and acid phosphatase. The concentration of PA in hybrid sunflower achenes varied from 2.16 to 2.83g/100g of sample (p < 0.05). The phytase and acid phosphatase activities of sunflowers BRS191 and C11 were the highest on the 4th and 5th days of germination, respectively, with the release of the phosphorus. These results indicated that hybrid sunflower PA reduced and enhance phytase activity at distinct germination periods, which could open up the possibility of applying these enzymes in the control of PA content in cereals, thus improving their nutritional value.

scholarly journals Uptake and translocation of nitrogen, phosphorus and calcium in soybean infected with Meloidogyne incognita and M. javanica

2002 ◽  
Vol 27 (2) ◽  
pp. 141-150 ◽  
Author(s):  
RUI G. CARNEIRO ◽  
PAULO MAZZAFERA ◽  
LUIZ CARLOS C.B. FERRAZ ◽  
TAKASHI MURAOKA ◽  
PAULO CESAR O. TRIVELIN
Keyword(s):  
Nutrient Uptake ◽  
Meloidogyne Incognita ◽  
Nutrient Concentrations ◽  
Tissue Mass ◽  
Split Root ◽  
Split Root System ◽  
Shoot Mass ◽  
Moderately Resistant ◽  
Nitrogen Phosphorus ◽  
Uptake And Transport

Two soybean (Glycine max) cultivars were used in this study, Ocepar 4, rated as moderately resistant to Meloidogyne incognita race 3 but susceptible to M. javanica, and 'BR 16', susceptible to both nematodes. The effect of nematodes infection on the uptake and transport of N, P and Ca to the shoot was studied in plants growing in a split root system. The upper half was inoculated with 0, 3,000, 9,000 or 27,000 eggs/plant while the lower half received 15N, 32P or 45Ca. Infected plants showed an increase of root but a decrease of shoot mass with increasing inoculum levels. In general, total endogenous nutrients increased in the roots and tended to decrease in the shoots with increasing inoculum levels. When concentrations were calculated, there was an increase in the three nutrients in the roots, and an increase of Ca but no significant variation of N and P was observed in the shoots. The total amount of 15N in the roots increased at the highest inoculum levels but 32P and 45Ca decreased. In the shoots there was a reduction of 32P and 45Ca. The specific concentrations of the labelled nutrients (abundance or radioactivity/tissue mass) also showed a decrease of 32P and 45Ca in the shoots and roots of infected plants and an increase of 15N in the shoots. Considering that overall nutrient concentrations reflect cumulative nutrient uptake and the data from labelled elements gave information at a specific moment of the infection, thus nematodes do interfere with nutrient uptake and translocation.

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