scholarly journals Betaine Improves Polymer-Grade D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as Green Neutralizer

2017 ◽  
Vol 66 (2) ◽  
pp. 273-276 ◽  
Author(s):  
Guoping Lv ◽  
Chengchuan Che ◽  
Li Li ◽  
Shujing Xu ◽  
Wanyi Guan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Fermentation Process ◽  
Specific Activity ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Ammonium Ion ◽  
High Concentration ◽  
Acid Fermentation ◽  
High Optical Purity

The traditional CaCO3-based fermentation process generates huge amount of insoluble waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralizer. The 106.7 g/l of D-lactic acid production and 0.89 g/g of consumed sugar were obtained by Sporolactobacillus inulinus CASD with a high optical purity of 99.7% by adding 100 mg/l betaine in the simple batch fermentation. The addition of betaine was experimentally proven to protect cell at high concentration of ammonium ion, increase the D-lactate dehydrogenase specific activity and thus promote the production of D-lactic acid.

scholarly journals Major Role of NAD-Dependent Lactate Dehydrogenases in the Production of l-Lactic Acid with High Optical Purity by the Thermophile Bacillus coagulans

2014 ◽  
Vol 80 (23) ◽  
pp. 7134-7141 ◽  
Author(s):  
Limin Wang ◽  
Yumeng Cai ◽  
Lingfeng Zhu ◽  
Honglian Guo ◽  
Bo Yu
Keyword(s):  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
Lactobacillus Delbrueckii ◽  
Content Type ◽  
High Optical Purity ◽  
Optically Pure

ABSTRACTBacillus coagulans2-6 is an excellent producer of optically purel-lactic acid. However, little is known about the mechanism of synthesis of the highly optically purel-lactic acid produced by this strain. Three enzymes responsible for lactic acid production—NAD-dependentl-lactate dehydrogenase (l-nLDH; encoded byldhL), NAD-dependentd-lactate dehydrogenase (d-nLDH; encoded byldhD), and glycolate oxidase (GOX)—were systematically investigated in order to study the relationship between these enzymes and the optical purity of lactic acid.Lactobacillus delbrueckiisubsp.bulgaricusDSM 20081 (ad-lactic acid producer) andLactobacillus plantarumsubsp.plantarumDSM 20174 (adl-lactic acid producer) were also examined in this study as comparative strains, in addition toB. coagulans. The specific activities of key enzymes for lactic acid production in the three strains were characterizedin vivoandin vitro, and the levels of transcription of theldhL,ldhD, and GOX genes during fermentation were also analyzed. The catalytic activities ofl-nLDH andd-nLDH were different inl-,d-, anddl-lactic acid producers. Onlyl-nLDH activity was detected inB. coagulans2-6 under native conditions, and the level of transcription ofldhLinB. coagulans2-6 was much higher than that ofldhDor the GOX gene at all growth phases. However, for the twoLactobacillusstrains used in this study,ldhDtranscription levels were higher than those ofldhL. The high catalytic efficiency ofl-nLDH toward pyruvate and the high transcription ratios ofldhLtoldhDandldhLto the GOX gene provide the key explanations for the high optical purity ofl-lactic acid produced byB. coagulans2-6.

scholarly journals Efficient Homofermentative l-(+)-Lactic Acid Production from Xylose by a Novel Lactic Acid Bacterium,Enterococcus mundtiiQU 25

2010 ◽  
Vol 77 (5) ◽  
pp. 1892-1895 ◽  
Author(s):  
Mohamed Ali Abdel-Rahman ◽  
Yukihiro Tashiro ◽  
Takeshi Zendo ◽  
Katsuhiro Hanada ◽  
Keisuke Shibata ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Acetate Production ◽  
High Optical Purity ◽  
Enterococcus Mundtii

ABSTRACTEnterococcus mundtiiQU 25, a newly isolated lactic acid bacterium, efficiently metabolized xylose intol-lactate. In batch fermentations, the strain produced 964 mMl-(+)-lactate from 691 mM xylose, with a yield of 1.41 mol/mol xylose consumed and an extremely high optical purity of ≥99.9% without acetate production.

scholarly journals Utilization of Molasses Sugar for Lactic Acid Production by Lactobacillus delbrueckii subsp. delbrueckii Mutant Uc-3 in Batch Fermentation

2007 ◽  
Vol 74 (1) ◽  
pp. 333-335 ◽  
Author(s):  
Arti Dumbrepatil ◽  
Mukund Adsul ◽  
Shivani Chaudhari ◽  
Jayant Khire ◽  
Digambar Gokhale
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Acid Production ◽  
Batch Fermentation ◽  
Lactic Acid Production ◽  
Lactobacillus Delbrueckii ◽  
Potential Candidate ◽  
Lactic Acid Concentration ◽  
High Concentration ◽  
Acid Fermentation

ABSTRACT Efficient lactic acid production from cane sugar molasses by Lactobacillus delbrueckii mutant Uc-3 in batch fermentation process is demonstrated. Lactic acid fermentation using molasses was not significantly affected by yeast extract concentrations. The final lactic acid concentration increased with increases of molasses sugar concentrations up to 190 g/liter. The maximum lactic acid concentration of 166 g/liter was obtained at a molasses sugar concentration of 190 g/liter with a productivity of 4.15 g/liter/h. Such a high concentration of lactic acid with high productivity from molasses has not been reported previously, and hence mutant Uc-3 could be a potential candidate for economical production of lactic acid from molasses at a commercial scale.

Optimal design of an integrated fermentation process for lactic acid production

AIChE Journal ◽  
2007 ◽  
Vol 53 (2) ◽  
pp. 449-459 ◽  
Author(s):  
Hsun-Tung Lin ◽  
Feng-Sheng Wang
Keyword(s):  
Lactic Acid ◽  
Optimal Design ◽  
Acid Production ◽  
Fermentation Process ◽  
Lactic Acid Production

Direct lactic acid fermentation: Focus on simultaneous saccharification and lactic acid production

2009 ◽  
Vol 27 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Rojan P. John ◽  
Anisha G.S. ◽  
K. Madhavan Nampoothiri ◽  
Ashok Pandey
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Lactic Acid Fermentation ◽  
Acid Fermentation ◽  
Simultaneous Saccharification

scholarly journals Dynamic Simulation of Continuous Mixed Sugars Fermentation with Increasing Cell Retention Time for Lactic Acid Production using Enterococcus mundtii QU 25

2020 ◽  
Author(s):  
Ying Wang ◽  
Ka-Lai Chan ◽  
Mohamed Ali Abdel-Rahman ◽  
Kenji Sonomoto ◽  
Shao-Yuan Leu
Keyword(s):  
Lactic Acid ◽  
Retention Time ◽  
Acid Production ◽  
Fermentation Process ◽  
Lactic Acid Production ◽  
Critical Role ◽  
Cell Retention ◽  
Mixed Sugars ◽  
Enterococcus Mundtii ◽  
Mixed Sugar

Abstract Background: Simultaneous and effective conversion of both pentose and hexoses in fermentation is a critical and challenging task toward the lignocellulosic economy. This study aims to investigate the feasibility of an innovative co-fermentation process featured with cell recycle unit (CF/CR) for mixed sugar utilization. A l-lactic acid producing strain Enterococcus mundtii QU 25 was applied in the continuous fermentation process to utilize the mixed sugar at different productivities over the changes of flowing conditions. Structured numerical platform were constructed with the experiments to optimize the biological process and clarify the cell metabolisms through kinetics analysis. The structured model, kinetic parameters, and achievement of the fermentation strategy shall provide new insights towards whole sugar fermentation via real-time monitoring for process control and optimization.Results: Significant carbon catabolite repression of co-fermentation using glucose/xylose mixture was overcome by replacing glucose with cellobiose, of which the consumption ratio of hexose to pentose was improved dramatically from 10.4:1 to 2.17:1. An outstanding product concentration of 65.15 g·L -1 and productivity of 13.03 g·L -1 ·h -1 were achieved with 50 g·L -1 cellobiose and 30 g·L -1 xylose, at an optimized dilution rate of 0.2 h -1 with gradually increased cell retention time. Among the total lactic acid production, xylose contributed to more than 34% of the mixed sugars, which was close to the related contents in agricultural residuals. The model successfully simulated the transition of sugar consumption, cell growth, and lactic acid production among the batch, continuous process, and CF/CR system.Conclusion: Cell retention time played a critical role in balancing pentose and hexose consumption, cell decay, and lactic acid production in the CF/CR process. With the increase of cell concentration, consumption of mixed sugars increased with the productivity of final products, hence the impacts of substrate inhibiting reduced. With the validated parameters, the model showed highest accuracy simulating the CF/CR process, of which significantly longer cell retention times over hydraulic retention time were tested.

scholarly journals Evaluation of conditions for fermentation of fish offal

1995 ◽  
Vol 4 (1) ◽  
pp. 11-17
Author(s):  
T. Mikael Lassén
Keyword(s):  
Lactic Acid ◽  
Redox Potential ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Storage Period ◽  
Growth Conditions ◽  
Inoculum Size ◽  
Reliable Estimate ◽  
Small Scale ◽  
Acid Fermentation

Conditions for the lactic acid fermentation of fish offal were evaluated regarding the effect of substrate concentration (2, 5 and 10% dextrose), preacidification with lactic acid (initial pH of 6.8, to 6.5 or 6.0), and inoculum size of Lactobacillus plantarum (107 , 108 and 109 colony forming units (cfu)/g). pH and lactic acid production were monitored during a two-week storage period. A small-scale silo for fermenting fish offal was also constructed, and measurement of redox potential was evaluated as a means to estimate bacterial growth conditions. The most favourable conditions for fermentation, manifested by a low and stable pH and high lactic acid production, were achieved with an inoculum size of 108 cfu/g and 5% dextrose. Preacidification did not affect final pH. Redox potential was shown to give a reliable estimate of growth conditions for bacteria under anaerobic conditions by rapidly falling to below -550mV in silage with a high lactic acid concentration.

L(+)-Lactic Acid Production in Plaque from Orthodontic Appliances Retained with Glass Ionomer Cement

1994 ◽  
Vol 21 (1) ◽  
pp. 23-26 ◽  
Author(s):  
A. Hallgren ◽  
A. Oliveby ◽  
S. Twetman
Keyword(s):  
Lactic Acid ◽  
Incubation Period ◽  
Acid Production ◽  
Orthodontic Treatment ◽  
Lactic Acid Production ◽  
Glass Ionomer Cement ◽  
Orthodontic Appliances ◽  
Glass Ionomer ◽  
Acid Fermentation ◽  
Ionomer Cement

The lactic acid production in suspensions of plaque collected adjacent to orthodontic brackets retained with a glass ionomer cement (GIC), or a resin-based composite was investigated using a split-mouth technique. Forty-eight-hour-old plaque was collected at 3, 8, and 28 days, and 3 months after the onset of orthodontic treatment. Acid fermentation was induced by glucose and the L(+)-lactic acid concentrations were determined enzymatically after a 30-minute incubation period. Significantly (P<0·05) lower levels of lactic acid were found in plaque from GIC-retained brackets compared with the composite controls at the 28 days and 3 months sampling occasions, respectively. The results suggest that the use of GIC as a bonding agent in orthodontics can be beneficial for patients assessed at risk of caries development.

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