Production of poultry feather hydrolysate using HCl and NaOH as a growth medium substrate for indigenous strains

The poultry feathers have a very high protein content due to it consists of 90% of crude protein, and it is an ideal material to obtain keratin protein. Due to Keratin’s difficulties and time-consuming decomposition, further processing is needed to degrade Keratin into simpler proteins that can be used as an alternative N-source. This study was aimed to evaluate the keratin hydrolysate from poultry feathers prepared by acidic (HCl) and alkaline (NaOH) compound utilization and its potency as the substrate medium for growth keratinolytic bacteria at a laboratory scale. Poultry feathers, including kampung (local breed) chicken feathers, layer chicken feathers, and local goose treat with HCL 12% and NaOH 20%. The results of the hydrolysate of poultry feathers using 12% HCl showed no significant changes. Visually, the feathers of birds that have been treated with 12% HCl show a colour change to brownish-yellow. The results of hydrolysis using NaOH showed better results than HCl for producing feather meals. The highest yield has occurred at local goose feathers at 95.7%, followed by Kampung and Layer chicken feathers at 93.17% and 78,75%. Based on the viability test, three indigenous strains (Bacillus cereus TD5B, B. cereus LS2B and Pseudomonas sp. PK4) grew in a medium with a substrate of kampung chicken feathers, layer chickens, and local goose feathers. It can be concluded that the hydrolysed poultry feathers made by NaOH 20% preparation had a potency as N-source in the bacterial growth medium.

The Effect of Neutralising Materials on the Reaction of the Peat Substrate Medium when Growing Ball-Rooted Seedlings

The use of various types of neutralising materials in the cultivation of standard planting material of high-quality forest wood species has a significant effect on substrate acidity. Therefore, the study sets a goal to select peat substrates with different dosages of the neutralising component and determine their effect on the reaction of the peat substrate medium. The paper presents the results of the influence of neutralising materials of dolomitic meal and chalk of various doses with the use of mineral fertilisers. Dolomitic meal was used as a neutralising component, the pH of the peat substrate was 6.6 and chalk was 7.5. It was found that when neutralising a peat substrate created based on high-moor milled peat (pH 2.5) with the introduction of dolomitic meal in doses of 2-4 kg/m3 (European spruce), 3-4. 5 kg/m3 (Scots pine), optimal acidity can be achieved in a month. An increase in the concentration of lime material was recorded, which leads to an increase in the neutralisation of the substrate at a dose of 2.5 g and 3.0 g per 125 g of peat. The best indicators were demonstrated by options of doses of 1.5-2.5 g per 125 g of peat for 7-8 days of use and a dose of 3.0 g per 125 g of peat for 5-6 days compared with the control. An increase in the concentration of chalk in the neutralising material from 30% to 70% leads to a faster deoxidation of the substrate (pH 3.2), and an increase in the dosage of chalk increases substrate neutralisation. The electrical conductivity of the substrate at a dosage of 6-8 kg/m3 when applying chalk and dolomitic meal increases by 1.6-2.0 and 1.2-1.4 times, respectively. At the rate of application of 6-10 kg/m3, it has a lesser effect on the change in the electrical conductivity of the high-moor peat, and the introduction of dolomitic meal 2-4.5 kg/m3 into the milled peat during neutralisation does not lead to a change in the electrical conductivity of the peat. This data allows choosing neutralising materials of a certain concentration that will enable the cultivation of standard planting material of European quality and thereby ensure the proper quality of future stands

Microgreens: a newly merging product, aspects, prospectives, and disadvantages

In this article we demonstrate the connotation of microgreens, the newly merging product in the Russian market. Microgreens are normal plants planted in highly density on a substrate medium and harvested shortly after the first true leaves appear. Microgreens of many aromatic plants possess intensive flavour similar to its mature product. We also expound the aspects related to this product, including growth, harvesting time, Seeds utilization, light requirements, available suitable substrate, as well as the disadvantages related to its production. There is still a lot of controversy about the health benefits of consuming microgreens. Some researchers believe that there is currently not enough scientific evidence to support a higher nutrient level in microgreens than in mature plants. In this review, we discuss whether microgreening is a great addition to gardening or not. Still, other prospects for the future of this product indicates that the demand of the market for the microgreens will be strong especially with the wide spread of home-growing facilities like phytotrons and simple growing chambers.

Comparative Study on Lead and Copper Biosorption Using Three Bioproducts from Edible Mushrooms Residues

Agricultural waste products can be used as biosorbents for bioremediation once they are low-cost and high-efficient in pollutants removal. Thus, waste products from mushroom farming such as cutting and substrate of Lentinula edodes (popularly known as shiitake) and Agaricus bisporus (also known as champignon) were evaluated as biosorbents for metallic contaminants copper (Cu) and lead (Pb). Shiitake and champignon stalks, and shiitake substrate (medium in which shiitake was cultivated) were dried, grounded, characterized and experimented to remove Cu and Pb from contaminated water. The Sips model was used to establish the adsorption isotherms. Regarding Cu, champignon stalks have the best removal efficiency (43%), followed by substrate and stalks of shiitake (37 and 30%, respectively). Pb removals were similar among three residues (from 72 to 83%), with the champignon stalks standing out. The maximum adsorption capacities (qmax) for Cu in shiitake and champignon stalks were 22.7 and 31.4 mg/g−1, respectively. For Pb, qmax for shiitake and champignon stalks, and shiitake substrate were 130.0, 87.0 and 84.0 mg/g−1, respectively. The surface morphology of the champignon stalks revealed an organized and continuous structure. After an interaction with metals, the stalk of champignon accumulated the metal ions into interstices. Mushroom residues showed a relevant adsorption efficiency, especially for Pb. Mushroom farming waste are a very low-cost and promising alternative for removing toxic heavy metals from aquatic environment.

Influence of Substrate Concentration on the Culturability of Heterotrophic Soil Microbes Isolated by High-Throughput Dilution-to-Extinction Cultivation

ABSTRACT The vast majority of microbes inhabiting oligotrophic shallow subsurface soil environments have not been isolated or studied under controlled laboratory conditions. In part, the challenges associated with isolating shallow subsurface microbes may persist because microbes in deeper soils are adapted to low nutrient availability or quality. Here, we use high-throughput dilution-to-extinction culturing to isolate shallow subsurface microbes from a conifer forest in Arizona, USA. We hypothesized that the concentration of heterotrophic substrates in microbiological growth medium would affect which microbial taxa were culturable from these soils. To test this, we diluted cells extracted from soil into one of two custom-designed defined growth media that differed by 100-fold in the concentration of amino acids and organic carbon. Across the two media, we isolated a total of 133 pure cultures, all of which were classified as Actinobacteria or Alphaproteobacteria. The substrate availability dictated which actinobacterial phylotypes were culturable but had no significant effect on the culturability of Alphaproteobacteria. We isolated cultures that were representative of the most abundant phylotype in the soil microbial community (Bradyrhizobium spp.) and representatives of five of the top 10 most abundant Actinobacteria phylotypes, including Nocardioides spp., Mycobacterium spp., and several other phylogenetically divergent lineages. Flow cytometry of nucleic acid-stained cells showed that cultures isolated on low-substrate medium had significantly lower nucleic acid fluorescence than those isolated on high-substrate medium. These results show that dilution-to-extinction is an effective method to isolate abundant soil microbes and that the concentration of substrates in culture medium influences the culturability of specific microbial lineages. IMPORTANCE Isolating environmental microbes and studying their physiology under controlled conditions are essential aspects of understanding their ecology. Subsurface ecosystems are typically nutrient-poor environments that harbor diverse microbial communities—the majority of which are thus far uncultured. In this study, we use modified high-throughput cultivation methods to isolate subsurface soil microbes. We show that a component of whether a microbe is culturable from subsurface soils is the concentration of growth substrates in the culture medium. Our results offer new insight into technical approaches and growth medium design that can be used to access the uncultured diversity of soil microbes.

The influence of substrate concentration on the culturability of heterotrophic soil microbes isolated by high-throughput dilution-to-extinction cultivation

The vast majority of microbes inhabiting oligotrophic shallow subsurface soil environments have not been isolated or studied under controlled laboratory conditions. In part, the challenges associated with isolating shallow subsurface microbes may persist because microbes in deeper soils are adapted to low nutrient availability or quality. Here we use high-throughput dilution-to-extinction culturing to isolate shallow subsurface microbes from a conifer forest in Arizona, USA. We hypothesized that the concentration of heterotrophic substrates in microbiological growth medium would affect which microbial taxa were culturable from these soils. To test this, we diluted extracted cells into one of two custom-designed defined growth media that differed only by a 100-fold difference in the concentration of amino acids and organic carbon. Across both media, we isolated a total of 133 pure cultures, all of which were classified as Actinobacteria or Alphaproteobacteria. The substrate availability dictated which actinobacterial phylotypes were culturable but had no significant effect on the culturability of Alphaproteobacteria. We isolated cultures that were representative of the most abundant phylotype in the soil microbial community (Bradyrhizobium spp.) and representatives of five of the top 10 most abundant Actinobacteria phylotypes, including Nocardioides spp., Mycobacterium spp., and several other phylogenetically-divergent lineages. Flow cytometry of nucleic acid-stained cells showed that cultures isolated on low-substrate medium had significantly lower nucleic-acid fluorescence than those isolated on high-substrate medium. These results show that dilution-to-extinction is an effective method to isolate abundant soil microbes and the concentration of substrates in culture medium influences the culturability of specific microbial lineages.ImportanceIsolating environmental microbes and studying their physiology under controlled conditions is an essential aspect of understanding their ecology. Subsurface ecosystems are typically nutrient-poor environments that harbor diverse microbial communities—the majority of which are thus far uncultured. In this study, we use modified high-throughput cultivation methods to isolate subsurface soil microbes. We show that a component of whether a microbe is culturable from subsurface soils is the concentration of growth substrates in the culture medium. Our results offer new insight into technical approaches and growth medium design that can be used to access the uncultured diversity of soil microbes.

The Displacement of the Diffraction Pattern in the Motion of the Medium

Diffraction of waves of different physical nature is one of the most important and studied phenomena of nature in connection with its versatile use in devices and measuring techniques. The influence of media motion on the diffraction pattern attracted the attention of researchers in connection with the development of the theory of electrodynamics of moving media and continues to attract attention in connection with a number of unsolved problems of physics. In this paper we consider a model of the diffraction of waves on the slit and lattice, located on a movable substrate (medium). Analytical relations for the calculation of the diffraction pattern intensity distribution are obtained. The significant influence of the medium motion on the diffraction pattern is established. The position of the zero-order maximum does not depend on the motion of the medium, on the one hand, the distance between the diffraction maxima decreases, on the other hand increases, until they disappear. The conditions under which the motion of the medium leads to an asymmetrical appearance of the diffraction intensity distribution, which is not observed in a stationary medium, are established. Generalized conditions of diffraction minima are obtained taking into account the motion of the medium. Shows the effect of the direction of the velocity of motion of the medium on the intensity of the diffraction pattern.

Pengaruh Pendinginan Daerah Perakaran terhadap Produksi Cabai (Capsicum annuum L.) di dalam Rumah Tanaman Kawasan Tropika

ABSTRACTCultivation of chili plants in the tropical greenhouse requires cooling as a solution of high temperature inside the greenhouse. One of the cooling system can be applied is root zone cooling. The aim of this research is to apply root zone cooling system to cultivation of chili plant in tropical greenhouse with substrate system, and to know the influence root zone cooling system on growth and productivity of chili plant. Plants are cultivated with a hydroponics substrate system using a drip irrigation system where the cooling pipe is buried inside the substrate medium. First treatment is cooling the root zone with cooled water and the substrate temperature of root zone cooling was 14.1-26.9 0C (CH treatment). Second treatment is cooling the root zone with no cooled water (ambient temperature), and the substrate temperature was 24.8-34.2 0C (NC treatment). The air temperature inside greenhouse was 29.4 0C and 24.7 0C, day and night. Due to lack of intensity of sunlight into the greenhouse, the plants sustain etiolating. However CH treatment produced higher fruit weight per plant compared with NC treatment. The weight of fruit per plant is 873.60 g and 546.00 g, for CH and NC respectively. The fruit size in the CH treatment also produced longer and heavier fruits than the NC treatment.Keywords: hydroponics of pepper, productivity, root temperatureABSTRAKBudidaya tanaman cabai di dalam rumah tanaman daerah beriklim tropis membutuhkan pendinginan sebagai solusi tingginya suhu udara di dalam rumah tanaman. Pendinginan yang dapat diterapkan salah satunya yaitu dengan pendinginan terbatas di daerah perakaran. Penelitian ini bertujuan untuk menerapkan teknik pendinginan daerah perakaran pada budidaya tanaman cabai di dalam rumah tanaman iklim tropika basah dengan sistem substrat, serta untuk mengetahui pengaruh pendinginan daerah perakaran pada pertumbuhan dan produktivitas tanaman cabai. Tanaman dibudidayakan dengan sistem hidroponik substrat dan sistem irigasi tetes yang di dalam media tanam dibenamkan pipa pendingin. Perlakuan pertama adalah pendinginan daerah perakaran dengan air yang didinginkan dan suhu media tanam yang didinginkan berkisar antara 14.1-26.9 0C (perlakuan CH). Perlakuan kedua adalah pendinginan daerah perakaran dengan air yang tidak didinginkan (suhu lingkungan) dengan kisaran suhu media tanam sebesar 24.8-34.2 0C (perlakuan NC), dengan suhu udara rata-rata 29.4 0C pada siang hari dan 24.7 0C pada malam hari. Tanaman mengalami etiolasi akibat kurangnya intensitas cahaya matahari yang masuk ke dalam rumah tanaman. Perlakuan CH menghasilkan bobot buah per tanaman yang lebih tinggi dibandingkan dengan perlakuan NC. Bobot buah per tanaman sebesar 873.60 g dan 546.00 g, untuk CH dan NC berturut-turut. Ukuran buah pun pada perlakuan CH menghasilkan buah yang lebih panjang dan lebih berat dari pada perlakuan NC.Kata kunci: hidroponik cabai, produktivitas, suhu zona perakaran