L(+)-Lactic Acid Production in Plaque from Orthodontic Appliances Retained with Glass Ionomer Cement

1994 ◽  
Vol 21 (1) ◽  
pp. 23-26 ◽  
Author(s):  
A. Hallgren ◽  
A. Oliveby ◽  
S. Twetman

The lactic acid production in suspensions of plaque collected adjacent to orthodontic brackets retained with a glass ionomer cement (GIC), or a resin-based composite was investigated using a split-mouth technique. Forty-eight-hour-old plaque was collected at 3, 8, and 28 days, and 3 months after the onset of orthodontic treatment. Acid fermentation was induced by glucose and the L(+)-lactic acid concentrations were determined enzymatically after a 30-minute incubation period. Significantly (P<0·05) lower levels of lactic acid were found in plaque from GIC-retained brackets compared with the composite controls at the 28 days and 3 months sampling occasions, respectively. The results suggest that the use of GIC as a bonding agent in orthodontics can be beneficial for patients assessed at risk of caries development.

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Miao Chen ◽  
Jianru Yi ◽  
Zhihe Zhao

Abstract Background White spot lesions (WSLs) often occur in orthodontic treatments. The objectives of this study were to develop a novel orthodontic cement using particles of nano silver (NAg), N-acetylcysteine (NAC) and 2-methacryloyloxyethyl phosphorylcholine (MPC), and to investigate the effects on bonding strength, biofilms and biocompatibility. Methods A commercial resin-modified glass ionomer cement (RMGIC) was modified by adding NAg, NAC and MPC. The unmodified RMGIC served as the control. Enamel bond strength and cytotoxicity of the cements were investigated. The protein repellent behavior of cements was also evaluated. The metabolic assay, lactic acid production assay and colony-forming unit assay of biofilms were used to determine the antibacterial capability of cements. Results The new bioactive cement with NAg, NAC and MPC had clinically acceptable bond strength and biocompatibility. Compared to commercial control, the new cement suppressed metabolic activity and lactic acid production of biofilms by 59.03% and 70.02% respectively (p < 0.05), reduced biofilm CFU by 2 logs (p < 0.05) and reduced protein adsorption by 76.87% (p < 0.05). Conclusions The new cement with NAg, NAC and MPC had strong antibacterial capability, protein-repellent ability and acceptable biocompatibility. The new cement is promising to protect enamel from demineralization during orthodontic treatments.


1960 ◽  
Vol 54 (2) ◽  
pp. 166-169
Author(s):  
K. Friis Jakobsen ◽  
T. Mann

1. A study was made of the effects of a milk diluent on bull, ram and boar spermatozoa. Respiration and fructolysis of spermatozoa were used as the two main criteria of sperm activity. The milk diluent was a standardized and commercially available milk product, consisting of sterilized and homogenized milk, supplemented with milk fat.2. The rate of oxygen uptake measured manometrically in the presence of air was increased by the addition of the milk diluent throughout the entire incubation period. Fructose utilization was assessed by the rate of lactic-acid production. The rate of the anaerobic lactic-acid formation was higher in the presence of the milk diluent during the later stages of incubation.3. The effect of the milk diluent on sperm respiration was most striking in experiments with the sperm-rich portion of boar ejaculate obtained by fractionated collection. A somewhat less marked effect was observed with bull semen, and in ram semen the effect was comparatively weak.


2009 ◽  
Vol 27 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Rojan P. John ◽  
Anisha G.S. ◽  
K. Madhavan Nampoothiri ◽  
Ashok Pandey

1992 ◽  
Vol 19 (3) ◽  
pp. 215-220 ◽  
Author(s):  
D. T. Millett ◽  
P. H. Gordon

Various factors influencing the survival time of orthodontic bands cemented to first permanent molars with glass ionomer cement (KETAC-CEM, Espe) have been investigated. Data on 513 patients with 1424 first molar bands who completed orthodontic treatment between 1985 and 1989 inclusive were analysed. Performance of bands cemented to first permanent molars varied according to the operator, age of the patient and treatment mechanics, but not according to the sex of the patient or quadrant in which the band was fitted.


1995 ◽  
Vol 4 (1) ◽  
pp. 11-17
Author(s):  
T. Mikael Lassén

Conditions for the lactic acid fermentation of fish offal were evaluated regarding the effect of substrate concentration (2, 5 and 10% dextrose), preacidification with lactic acid (initial pH of 6.8, to 6.5 or 6.0), and inoculum size of Lactobacillus plantarum (107 , 108 and 109 colony forming units (cfu)/g). pH and lactic acid production were monitored during a two-week storage period. A small-scale silo for fermenting fish offal was also constructed, and measurement of redox potential was evaluated as a means to estimate bacterial growth conditions. The most favourable conditions for fermentation, manifested by a low and stable pH and high lactic acid production, were achieved with an inoculum size of 108 cfu/g and 5% dextrose. Preacidification did not affect final pH. Redox potential was shown to give a reliable estimate of growth conditions for bacteria under anaerobic conditions by rapidly falling to below -550mV in silage with a high lactic acid concentration.


2009 ◽  
Vol 25 (6) ◽  
pp. 703-708 ◽  
Author(s):  
Kazuko Nakajo ◽  
Satoshi Imazato ◽  
Yusuke Takahashi ◽  
Wakako Kiba ◽  
Shigeyuki Ebisu ◽  
...  

BioResources ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 1379-1389
Author(s):  
Yingnan Cao ◽  
Juan Wang ◽  
Qunhui Wang ◽  
Jianguo Liu ◽  
Tingxi Liu ◽  
...  

Efficient pretreatment and enzymatic hydrolysis is critical to achieve effective utilization of lignocellulosic biomass. In this study, the cellulase composition for lignocellulosic biomass hydrolysis was strategically optimized to improve the efficiency of vinasse saccharification and thus enhance L-lactic acid production. The results showed that the supplementation of β-glycosidase (BG) increased sugar production, and the glucose concentration exceeded cellobiose concentration after 48 h of hydrolysis. These results suggested that the addition of BG aided the hydrolysis of cellobiose and reduced the inhibitory effects caused by sugar accumulation. After 72 h to 96 h of hydrolysis, the BG supplementation improved cellobiose and glucose production by 25.7% and 27.4%, respectively. The effect of BG supplementation on L-lactic acid production during the fermentation of microwave-alkali pretreated vinasse was also investigated. Here, the L-lactic acid production from simultaneous saccharification and fermentation (SSF) with the addition of BG was 20.8% higher than that without BG addition, and was also 37.0% higher than production from separate hydrolysis and fermentation with BG addition. These results indicated the utilization efficiency of lignocellulosic biomass for L-lactic acid production could be enhanced by supplementation of BG in SSF.


2014 ◽  
Vol 17 (2) ◽  
pp. 65-71
Author(s):  
Nhat Minh Dang ◽  
Trung Quang Nguyen

This study aimed to evaluate the effect of various factors on the yield of lactic acid fermentation using sap from sugar palm and Lactobacillus casei. The sugar palm sap after harvesting, pretreatment was added with ammonium sulphate, calcium carbonate and microbial culture at density of 109 cells/ml and let fermented for acid lactic production. The results of the experiments showed that the culture size, amount of added ammonium sulphate and calcium carbonate had significant effect on lactic acid production. The most appropriate parameters determined were culture size of 5%, ammonium sulphate of 3.0 g/l and calcium carbonate of 4.0 g/l. Meanwhile, the optimum period of fermentation was 100 h, which gave the yield of lactic acid production of 22.30 g. Lactobacillus casei was considered to have lower ability to effectively produce lactic acid from sugar palm sap compared to Lactobacillus plantarum


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