scholarly journals Pink Lotus Essential Oil and Alleviates on Free Fatty Acid Induced Steatosis in HepG2 Cells via PI3K/Akt and NF-κB Pathways

2022 ◽  
Vol 71 (1) ◽  
pp. 95-104
Author(s):  
Runzhou Sun ◽  
Ruixin Xiao ◽  
Pengfei Lv ◽  
Feifei Guo ◽  
Yanling Gong ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Essential Oil ◽  
Hepg2 Cells

Cichoric Acid Prevents Free-Fatty-Acid-Induced Lipid Metabolism Disorders via Regulating Bmal1 in HepG2 Cells

2018 ◽  
Vol 66 (37) ◽  
pp. 9667-9678 ◽  
Author(s):  
Rui Guo ◽  
Beita Zhao ◽  
Yijie Wang ◽  
Dandan Wu ◽  
Yutang Wang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Free Fatty Acid ◽  
Hepg2 Cells ◽  
Cichoric Acid ◽  
Lipid Metabolism Disorders

Preventive Effect of Geniposide on Metabolic Disease Status in Spontaneously Obese Type 2 Diabetic Mice and Free Fatty Acid-Treated HepG2 Cells

2011 ◽  
Vol 34 (10) ◽  
pp. 1613-1618 ◽  
Author(s):  
Kazuko Kojima ◽  
Tsutomu Shimada ◽  
Yasuhiro Nagareda ◽  
Michiru Watanabe ◽  
Junko Ishizaki ◽  
...  
Keyword(s):  
Metabolic Disease ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Hepg2 Cells ◽  
Disease Status ◽  
Diabetic Mice ◽  
Preventive Effect ◽  
Type 2 Diabetic

Effect and mechanism of ginsenoside Rg1-regulating hepatic steatosis in HepG2 cells induced by free fatty acid

2020 ◽  
Vol 84 (11) ◽  
pp. 2228-2240
Author(s):  
Yue Gao ◽  
Shujun Zhang ◽  
Jiajun Li ◽  
Jinqiu Zhao ◽  
Qing Xiao ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Hepatic Steatosis ◽  
Hepg2 Cells ◽  
Ginsenoside Rg1

scholarly journals An Experimental Model of Hepatic Steatosis to Detect Lipid Accumulation

2018 ◽  
pp. 1-10
Author(s):  
Maryam Ammani Lawal ◽  
Gregory Elayeche Oko ◽  
Emmanuel Paul Okoi ◽  
Khuyen Thi Kim Vo ◽  
Shuaib Samirah Isah
Keyword(s):  
Insulin Resistance ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Hepatic Steatosis ◽  
Experimental Model ◽  
Hepg2 Cells ◽  
Colorimetric Detection ◽  
Acid Media ◽  
Alcoholic Fatty Liver ◽  
Increased Risk

Obesity is associated with an increased risk of metabolic syndromes such as type 2 diabetes, insulin resistance, dyslipidaemia and non-alcoholic fatty liver disease. Obesity occurs as a result of an imbalance between food intake and energy expenditure leading to excessive accumulation of adipose tissue. NAFLD is the most common liver condition and related to the resistance of insulin. Insulin resistance is associated with an increased influx of lipid into the liver promoting accumulation of hepatic triglyceride. This study aims to develop an experimental model of hepatic steatosis with lipid over-accumulation. HepG2 cells were cultured for 24 hours in free fatty acid media (1:2 palmitic acid and oleic acid respectively). Intracellular lipid content and lipotoxicity were determined by oil red O staining followed by colorimetric detection. This experiment was accomplished by defining the experimental conditions of lipid exposure that leads to significant intracellular fat accumulation in the absence of lipotoxicity with 1 mM of free fatty acid media. As a result, oleic and palmitic acids could be over-accumulated in HepG2 cells. 1 mM free fatty acid media did not affect the cell integrity and did not cause lipotoxicity of the cells.

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