scholarly journals Biochemical Compositions of Follicular Fluid and the Effects of Culture Conditions on the In Vitro Development of Pig Oocytes

2002 ◽  
Vol 15 (10) ◽  
pp. 1403-1411 ◽  
Author(s):  
Wei-Tung Huang ◽  
She-Ghi Lu ◽  
Pin-Chi Tang ◽  
Shinn-Chih Wu ◽  
San-Pao Cheng ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Culture Conditions ◽  
In Vitro Development ◽  
Biochemical Compositions ◽  
Vitro Development

scholarly journals Bioreactors as physiologicallike in vitro models

2020 ◽  
Author(s):  
Sara Mantero ◽  
Federica Boschetti
Keyword(s):  
Culture Conditions ◽  
In Vitro Models ◽  
In Vivo Models ◽  
In Vitro Development ◽  
Culture Cells ◽  
Vitro Development ◽  
Tissue Models ◽  
Mechanical Stretching

Bioreactors are powerful tools for in vitro development of engineered substitutes through controlled biological, physical, and mechanical culture conditions: bioreactor technology allows a closer in vitro replication of native tissues. One of bioreactors applications is the design of in vitro 3D tissue models as a bridge between 2D and in vivo models, allowing the application of 3R (replacement, reduction, refinement) principle. To this aim, bioreactors can be used to culture cells seeded on engineered scaffolds under in vivo-like conditions. Another key use of bioreactors is for perfusion decellularization of tissues and organs to be used as scaffolds. This contribution describes a dynamic stretching. bioreactor, imposing a mechanical stretching to the cultured constructs, allowing the development of skeletal muscle engineered constructs, and a decellularization bioreactor, designed for decellularization of blood vessels.

In vitro development of morulae from immature caprine oocytes

Zygote ◽  
1994 ◽  
Vol 2 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Levent Keskintepe ◽  
Gamal M. Darwish ◽  
Abdelmoneim I. Younis ◽  
Benjamin G. Brackett
Keyword(s):  
Oocyte Maturation ◽  
Follicular Fluid ◽  
Glycoprotein Hormones ◽  
Glycoprotein Hormone ◽  
In Vitro Development ◽  
Morula Stage ◽  
Vitro Development ◽  
Hormone Control ◽  
Medium Supplementation

SummaryThe effects of medium supplementation with oestrous goat serum and glycoprotein hormones on caprine oocyte maturation in vitro (IVM) were evidenced by proportions of resulting ova completing in vitro fertilisation (IVF) and development to the morula stage. Oocyte-cumulus complexes (OCCs) were harvested in follicular fluid from 2–5 mm diameter follicles. Oocyte maturation took place during 27 h in TCM-199 supplemented with 20% oestrous goat serum, oestradiol-17β (1.0 μg/ml), and either (a) 0.5 μg FSH/ml, (b) 100 μg LH/ml, (c) 100 μg LH + 0.5 μg FSH/ml, (d) 100 μg hCG + 0.5 μg FSH/ml, (e) 0.5 μg TSH/ml or (f) no added glycoprotein hormone (control). Of 353 immature oocytes cultured in seven experiments, 311 (88.1%) exhibited cumulus expansion at the end of the IVM interval; all normalappearing OCCs were inseminated. In vitro insemination was with ejaculated sperm treated with heparin (10 μg/ml) and caffeine (0.4 μg/ml). Proportions (%) of inseminated ova that were fertilised (cleaved) and that reached the morula stage after IVM with (a) FSH, (b) LH, (c) LH + FSH, (d) hCG + FSH, (e) TSH and (f) no added glycoprotein hormone were (a) 22/52 (42.3%) and 9/52 (17.3%), (b) 25/54 (46.3%) and 14/54 (25.9%), (c) 52/65 (80.0%) and 26/65 (40.0%), (d) 48/78 (61.5%) and 22/78 (28.2%), (e) 14/54 (25.9%) and 4/54 (7.4%), and (f) 11/50 (22.0%) and 1/50 (2.0%), respectively. All treatments yielded better results than IVM with no added glycoprotein hormone. After IVM with added LH + FSH higher proportions of oocytes were fertilised (p<0.05), and higher proportions reached the morula stage (p<0.05) when compared with other treatments.

Influence of follicular fluid from different sources on in vitro development of bovine embryos produced in vitro

1997 ◽  
Vol 47 (1) ◽  
pp. 292
Author(s):  
C Larocca ◽  
S Kmaid ◽  
I Lago ◽  
G Roses ◽  
D Fila ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Vitro Development ◽  
Different Sources

scholarly journals Effect of Different Culture Conditions on In vitro Development and Quality of Buffalo Embryos

2018 ◽  
Vol 46 (1) ◽  
pp. 70-78
Author(s):  
Fatma Ibrahim ◽  
Hassan Mansour ◽  
Faten Labib ◽  
Hussein Amer ◽  
Magdy Badr
Keyword(s):  
Culture Conditions ◽  
In Vitro Development ◽  
Vitro Development

Bovine dominant follicular fluid promotes the in vitro development of goat preantral follicles

2012 ◽  
Vol 24 (3) ◽  
pp. 490 ◽  
Author(s):  
A. B. G. Duarte ◽  
V. R. Araújo ◽  
R. N. Chaves ◽  
G. M. Silva ◽  
D. M. Magalhães-Padilha ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Culture Medium ◽  
Follicular Development ◽  
Ultrastructural Analysis ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Nuclear Membranes ◽  
Vitro Development ◽  
First Time

The aim of this study was to evaluate the effect of follicular fluid collected from bovine dominant follicles (bFF) on the in vitro development of goat preantral follicles and determine the best time to add this supplement to the culture medium. The preantral follicles were isolated and randomly distributed into four treatments in absence (control) or presence of 10% of bFF added on Days 0 (FF0–18), 6 (FF6–18) or 12 (FF12–18) of culture onwards. After 18 days, follicular development was assessed based on follicular survival, antral cavity formation, increased follicular diameter as well as fully grown oocyte (>110 μm) viability and meiosis resumption. The oocytes from the cultured follicles were in vitro-matured and processed for fluorescence or ultrastructural analysis. The results showed that on Day 18 the treatment FF0–18 had a significantly higher (P < 0.05) survival than control and FF12–18, but not FF6–18. The addition of bFF at the beginning of culture (FF0–18 and FF6–18) promoted a high percentage of follicular growth, meiosis resumption and early antrum formation. Moreover, this study described for the first time the ultrastructural analysis of caprine oocytes grown in vitro. This evaluation revealed that in the presence of bFF on (FF0–18) the in vitro-grown oocytes presented normal organelle distribution and well-defined, intact plasma and nuclear membranes. In conclusion, bFF originating from dominant follicles maintain the survival and promote the in vitro growth of goat preantral follicles when added at the beginning of culture.

Interaction between embryos and culture conditions during in vitro development of bovine early embryos

Zygote ◽  
2008 ◽  
Vol 16 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Yoshikazu Nagao ◽  
Rumi Iijima ◽  
Kazuhiro Saeki
Keyword(s):  
Oxygen Concentration ◽  
Inorganic Phosphate ◽  
Drop Size ◽  
Culture Conditions ◽  
Cooperative Interaction ◽  
Blastocyst Development ◽  
In Vitro Development ◽  
Early Embryos ◽  
Vitro Development

SummaryVarious factors such as embryo density and substances in the medium can influence embryo development in vitro. These factors and the embryos probably interact with each other, however the interactions are not fully understood. To investigate the interactions, we examined the effects of the number of embryos, drop size, oxygen concentration and glucose and inorganic phosphate in the medium during protein-free culture of bovine IVM/IVF embryos. In Experiment 1, different numbers of embryos were cultured in a 50 μl drop of medium. The frequencies of blastocyst development in the groups with 25, 50 and 100 embryos per drop were higher than in the other groups. One, five and 25 embryos were cultured in different drop sizes (Experiment 2), a 50 µl drop of medium at different O2 concentrations (Experiment 3) and a 50 µl drop of medium excluding glucose and/or inorganic phosphate (Experiment 4). In Experiment 2, the size of the medium drops did not improve blastocyst development. In Experiment 3, the highest frequency of blastocyst development for one, five and 25 embryos per drop was obtained at 1, 2.5 and 5% O2, respectively. In Experiment 4, blastocyst development for one and five embryos per drop were improved in the medium excluded inorganic phosphate. These results indicate that there is a cooperative interaction among embryos during culture and that this interaction may be mediated by reduction of toxic factors in the medium. At low embryo density, reduced oxygen concentration or the exclusion of inorganic phosphate enhanced blastocyst development.

Effect of culture conditions, donor source, and injection site on in vitro development of DNA injected porcine zygotes

1994 ◽  
Vol 41 (1) ◽  
pp. 211 ◽  
Author(s):  
M.A. Hajdu ◽  
J.W. Knight ◽  
R.S. Canseco ◽  
R.L. Krisher ◽  
J.L. Johnson ◽  
...  
Keyword(s):  
Injection Site ◽  
Culture Conditions ◽  
In Vitro Development ◽  
Vitro Development ◽  
Donor Source

159 IN VITRO DEVELOPMENT OF CAPRINE EMBRYO USING CRYOPRESERVED BLACK BENGAL BUCK SEMEN

2017 ◽  
Vol 29 (1) ◽  
pp. 188
Author(s):  
R. Kumar ◽  
P. Chandra ◽  
P. Konyak ◽  
M. Karunakaran ◽  
A. Santra ◽  
...  
Keyword(s):  
Embryo Development ◽  
Follicular Fluid ◽  
Saline Solution ◽  
Cumulus Cells ◽  
Sodium Pyruvate ◽  
In Vitro Development ◽  
Vitro Development ◽  
Further Development ◽  
Maximum Humidity

The purpose of this study was to check the competence of cryopreserved black Bengal buck semen to produce goat embryo through IVF. So far, cryopreserved black Bengal buck semen has not been used to produce goat embryos by IVF. For the study, fresh goat oviducts and ovaries were collected from slaughterhouse in a thermo flask containing 0.9% saline solution supplemented with antibiotic (400 IU mL−1 penicillin and 500 mg mL−1 streptomycin) at 30–35°C and transported to laboratory within 2–3 h of slaughter. Cumulus-oocyte complexes were collected from slaughterhouse ovaries, washed 5–6 times, and cultured in maturation media (TCM-199 + 10% FBS + 10 mg mL−1 FSH-P + 0.81 mM sodium pyruvate + 5% follicular fluid + 50 mg mL−1 gentamicin sulfate + 1 μg mL−1 oestradiol + 100 μM cysteamine) for 27 h in a 5% CO2 incubator at 38.5°C with maximum humidity. After 27 h of culture, cumulus cells were separated from matured oocytes by repeated pipetting using a fine pipette in fertilization Bracket and Oliphant’s (BO) medium. After removal of cumulus cells, the oocytes were transferred to acidified Tyrode’s medium for zona thinning for 52 s and were co-incubated with capacitated sperms for fertilization in fertilization BO medium at 38.5°C in 5% CO2 in air with maximum humidity. In the experiment I, freshly collected buck semen was used for IVF after processing for capacitation. In experiment II, cryopreserved buck semen straws were thawed and sperm were capacitated in vitro and used for fertilization. After 5 h of co-incubation, presumptive zygotes were washed thoroughly and cultured in embryo development medium for cleavage. Three different in vitro development media (RVCL, mSOF + 2.5% BSA, and KSOM + 0.5% BSA) were used. After 40 to 42 h, cleavage was observed and embryos were co-incubated with oviducal cells in replacement media for further development. In the fresh group, overall cleavage rates (%) were 37.76 ± 2.98, 39.60 ± 1.75, 29.01 ± 1.74 and morula formation rates (%) were 7.72 ± 3.38, 6.03 ± 1.29, 3.00 ± 3.00 in RVCL, mSOF, and KSOM media, respectively. However, in the cryopreserved group the overall cleavage rates (%) were 29.17 ± 2.56, 27.70 ± 2.31, and 24.17 ± 1.44 in RVCL, mSOF, and KSOM media, respectively, and morula formation (%) was achieved 2.93 ± 0.97 in RVCL media. These results indicate that cryopreserved black Bengal buck semen have competence to produce embryos and could be used for embryo development through IVF.

scholarly journals Effect of culture conditions, donor age, and injection site on in vitro development of DNA microinjected porcine zygotes1

1994 ◽  
Vol 72 (5) ◽  
pp. 1299-1305 ◽  
Author(s):  
M. A. Hajdu ◽  
J. W. Knight ◽  
R. S. Canseco ◽  
R. L. Krisher ◽  
W. H. Velander ◽  
...  
Keyword(s):  
Injection Site ◽  
Culture Conditions ◽  
Donor Age ◽  
In Vitro Development ◽  
Vitro Development
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