scholarly journals Systematic design, generation, and application of synthetic datasets for flow cytometry

2022 ◽  
pp. pdajpst.2021.012659
Author(s):  
Melissa Cheung ◽  
Jonathan J Campbell ◽  
Robert J Thomas ◽  
Julian Braybrook ◽  
Jon Petzing
Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S38-S39
Author(s):  
M. Cheung ◽  
J.J. Campbell ◽  
J. Braybrook ◽  
R. Thomas ◽  
J. Petzing

Author(s):  
Michael Elwert ◽  
Manuel Ramsaier ◽  
Boris Eisenbart ◽  
Ralf Stetter

AbstractGraph-based design languages offer a promising approach to address several major issues in engineering, e. g. the laborious manual transfer between CAD and CAE. Such languages generate a digital meta- or system model storing all relevant information about a design and feed this into any relevant CAE tool as needed to simulate and test the impact of any design variation on the resulting product performance. As this can be automated in digital compilers to perform systematic design variation for an almost infinite amount of parameters, such graph-based languages are a powerful means to generate viable design alternatives and thus permit fast evaluations.To leverage the full potential of graph-based design languages, possibilities are presented to expand their applicability into the domain of product functions. This possibilities allow to cohesively link integrative function modelling to product structures. This intends to close the gap between the early, abstract stages and the systematic, concrete design generation and validation with relevant CAE tools. In this paper, the IFM Framework was selected as integrated function model to be linked with the graph- based design languages.


2001 ◽  
Vol 66 (2) ◽  
pp. 100-106 ◽  
Author(s):  
M. Bellido ◽  
E. Rubiol ◽  
J. Ubeda ◽  
O. Lopez ◽  
C. Estivill ◽  
...  

1991 ◽  
Vol 65 (04) ◽  
pp. 432-437 ◽  
Author(s):  
A W J Stuttle ◽  
M J Powling ◽  
J M Ritter ◽  
R M Hardisty

SummaryThe anti-platelet monoclonal antibody P256 is currently undergoing development for in vivo detection of thrombus. We have examined the actions of P256 and two fragments on human platelet function. P256, and its divalent fragment, caused aggregation at concentrations of 10−9−3 × 10−8 M. A monovalent fragment of P256 did not cause aggregation at concentrations up to 10−7 M. P256–induced platelet aggregation was dependent upon extracellular calcium ions as assessed by quin2 fluorescence. Indomethacin partially inhibited platelet aggregation and completely inhibited intracellular calcium mobilisation. Apyrase caused partial inhibition of aggregation. Aggregation induced by the divalent fragment was dependent upon fibrinogen and was inhibited by prostacyclin. Aggregation induced by the whole antibody was only partially dependent upon fibrinogen, but was also inhibited by prostacyclin. P256 whole antibody was shown, by flow cytometry, to induce fibrinogen binding to indomethacin treated platelets. Monovalent P256 was shown to be a specific antagonist for aggregation induced by the divalent forms. In–111–labelled monovalent fragment bound to gel-filtered platelets in a saturable and displaceable manner. Monovalent P256 represents a safer form for in vivo applications


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