scholarly journals Antibacterial Effect of Silver Nanoparticles Versus Chlorhexidine Against Streptococcus mutans and Lactobacillus casei

Author(s):  
Raul Alberto Morales Luckie ◽  
Rafael Lopez Casatañares ◽  
Rogelio Schougall ◽  
Sarai Carmina Guadarrama Reyes ◽  
Víctor Sanchez Mendieta

2009 ◽  
Vol 63 (29) ◽  
pp. 2603-2606 ◽  
Author(s):  
L.F. Espinosa-Cristóbal ◽  
G.A. Martínez-Castañón ◽  
R.E. Martínez-Martínez ◽  
J.P. Loyola-Rodríguez ◽  
N. Patiño-Marín ◽  
...  


2019 ◽  
Vol 9 (2) ◽  
pp. 13-19
Author(s):  
S Hima Bindu ◽  
S V Kala Vani ◽  
G Nirisha ◽  
N Madhuri ◽  
B Sai Deepa ◽  
...  

Correction: On 23rd April 2020, corrections were made to page 16: The caption of Figure 6 (p.16) was changed FROM Figure 4: Petridishes showing Lactobacillus colonies of control samples at (a) 24 hours, (b) 48 hours and (c) 1 week TO Figure 6: Petridishes showing streptococcus mutans colonies of control group at (a) 24 hours, (b) 48 hours and (c) 1 week. The caption of Figure 7 (p.16) was changed FROM Figure 5: Petridishes showing Lactobacillus colonies of test samples at (a) 24 hours, (b) 48 hours and (c) 1 week TO Figure 7: Petridishes showing Streptococcus mutans colonies of test samples at (a) 24 hours (b) 48 hours and (c) 1 week. AbstractIntroduction: Decalcification, caries, inflammatory periodontal disease are the most common iatrogenic effects of orthodontic treatment because of failure to maintain proper oral hygiene. Although various methods have been tried to minimize the incidence of white spot lesions, none of them proved to be effective. The purpose of this study was to develop a hard coating of silver nanoparticles on stainless steel band material and to evaluate the antibacterial efficacy against most common cariogenic pathogens. Materials & Method: Stainless steel band material was cut into 45 pieces of about 0.5 x 1 cm in dimension, of these 25 band material strips were coated with silver nanoparticles using thermal evaporation technology in a Vacuum coating unit (Indovision, India) at a vacuum of 4.5 ×10−5 millibar at 961°C for 5 minutes and remaining strips served as control. Scanning electron microscopy (SEM) study of coated band material showed a uniform deposition of silver nanoparticles of about 18.63 percent by weight. Five coated and five uncoated band material strips were utilized for each test to evaluate the antibacterial effect of the coated band material against Streptococcus mutans, Lactobacillus acidophilus using zone of inhibition and direct contact test. In zone of inhibition test, the bacterial growth inhibition zone was measured after a period of 24-48 hours, where as in direct contact test, the number of bacterial colonies were counted after 24 hours, 48 hours and 1 week. Five coated band materials were immersed separately in a container having 5 ml of artificial saliva and the amount of silver nanoparticles released from coated samples was evaluated after 24 hrs, 48 hrs, and 1 week using atomic absorption spectrophotometer. Result: A stable uniform coating of silver nanoparticles on the band material was obtained by physical vapor deposition. The coated band material showed a potent antibacterial activity against L.acidophilus and S.mutans. The maximum amount of silver nanoparticles released from the silver nanoparticle coated band material was 0.0236 ± 0.0067 ppm, which is below the maximum permissible level set by WHO [0.1 mg /l], proving it as biocompatible. Conclusion: Silver nanoparticle coating on orthodontic band surfaces can provide suitable antimicrobial activity during active orthodontic treatment.



2020 ◽  
Vol 36 (4) ◽  
pp. 87-93
Author(s):  
V.Yu. Reshetova ◽  
A.F. Krivoshchepov ◽  
I.A. Butorova ◽  
N.B. Feldman ◽  
S.V. Lutsenko ◽  
...  

Chitosan beads with colloidal silver nanoparticles inclued in the polymer matrix have been obtained by the introduction of chitosan into an acidified nanosilver sol. Dual interconnection of drops of the resulting solution was then carried out by ionotropic gelation at the first stage and covalent crosslinking of the polymer matrix with adipic acid at the second stage. The surface morphology of the obtained beads was studied by scanning electron microscopy. Data of Fourier transform IR spectroscopy confirmed the formation of covalent bonds between chitosan and adipic acid. The antibacterial activity of obtained beads against S. aureus and E. coli was evaluated using agar diffusion test. It was shown that the сhitosan beads modified with nanostructured silver exhibited an antibacterial effect against the tested strains, and they can be used as a basis for creating biodegradable wound healing dressings with a prolonged antibacterial effect. chitosan, silver nanoparticles, antibacterial activity, wound dressings This work was supported by the "Russian Academic Excellence Project 5-100". The study was carried out with the financial support of the Russian Foundation for Basic Research in the framework of the Scientific Project no. 18-29-18039.





Molecules ◽  
2011 ◽  
Vol 16 (8) ◽  
pp. 6667-6676 ◽  
Author(s):  
Mohsen Zargar ◽  
Azizah Abdul Hamid ◽  
Fatima Abu Bakar ◽  
Mariana Nor Shamsudin ◽  
Kamyar Shameli ◽  
...  


2011 ◽  
Vol 10 (52) ◽  
pp. 10795-10797 ◽  
Author(s):  
Habibian Dehkordi Saied ◽  
Hosseinpour Fatemeh ◽  
Ebrahimi Kahrizangi Azizollah


Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2400
Author(s):  
Afrah E. Mohammed ◽  
Lamya Ahmed Al-Keridis ◽  
Ishrat Rahman ◽  
Modhi O. Alotaibi ◽  
Rasha Saad Suliman ◽  
...  

The broad application of metal nanoparticles in different fields encourages scientists to find alternatives to conventional synthesis methods to reduce negative environmental impacts. Herein, we described a safe method for preparing silver nanoparticles (J-AgNPs) using Jatropha integerrima leaves extract as a reducing agent and further characterize its physiochemical and pharmacological properties to identify its therapeutic potential as a cytotoxic and antimicrobial agent. The biogenic synthesized J-AgNPs were physiochemically characterized by ultraviolet-visible spectroscopy, dynamic light scattering (DLS), transmission electron microscope (TEM), and energy-dispersive X-ray spectroscopy. HPLC-DAD, followed by LC/MS and the Fourier-transform infrared spectroscopy (FTIR), was applied to detect the biomolecules of J. integerrima involved in the fabrication of NPs. Furthermore, J-AgNPs and the ampicillin-nanocomposite conjugate were investigated for their potential antibacterial effects against four clinical isolates. Finally, cytotoxic effects were also investigated against cancer and normal cell lines, and their mechanism was assessed using TEM analysis and confocal laser scanning microscopy (LSM). Ag ions were reduced to spherical J-AgNPs, with a zeta potential of −34.7 mV as well as an average size of 91.2 and 22.8 nm as detected by DLS and TEM, respectively. HPLC GC/MC analysis identified five biomolecules, and FTIR suggested the presence of proteins besides polyphenolic molecules; together, these molecules could be responsible for the reduction and capping processes during NP formation. Additionally, J-AgNPs displayed a strong antibacterial effect, although the ampicillin conjugated form had a very weak antibacterial effect. Furthermore, the NPs caused a reduction in cell viability of all the treated cells by initiating ultrastructural changes and apoptosis, as identified by TEM and LSM analysis. Therefore, J-AgNPs can be formed using the leaf extract from the J. integerrima plant. Furthermore, J-AgNPs may serve as a candidate for further biochemical and pharmacological testing to identify its therapeutic value.



Sign in / Sign up

Export Citation Format

Share Document