Nucleic Acids for Electrochemical Biosensor Technology

Biosensor technology has developed extremely rapidly in recent years. This technology brings along precise measurements as well as specific measurements. Thanks to its ability to be miniaturized and be easily accessible to the end user, it is one-step ahead of other similar methods. The selectivity of biological molecules and the sensitivity of electrochemical methods enable the continuous evolvement of these new technologies. In this chapter, the use of nucleic acids as both recognition agents and target molecules, the way they are used in biosensor technology and their electrical properties are explained in detail with examples. Aptamers, which are synthetic nucleic acids, and their use in electrochemical biosensor systems with different electrochemical and immobilization methods have been compared extensively.

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Modified Nucleosides, Nucleotides and Nucleic Acids via Click Azide-Alkyne Cycloaddition for Pharmacological Applications

Molecules ◽

10.3390/molecules26113100 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3100

Author(s):

Daniela Perrone ◽

Elena Marchesi ◽

Lorenzo Preti ◽

Maria Luisa Navacchia

Keyword(s):

Cancer Therapy ◽

Nucleic Acids ◽

Click Chemistry ◽

Nucleoside Analogs ◽

Modified Nucleosides ◽

Biological Molecules ◽

Dipolar Cycloaddition ◽

Virus Diseases ◽

Reaction Conditions ◽

Hybrid Compounds

The click azide = alkyne 1,3-dipolar cycloaddition (click chemistry) has become the approach of choice for bioconjugations in medicinal chemistry, providing facile reaction conditions amenable to both small and biological molecules. Many nucleoside analogs are known for their marked impact in cancer therapy and for the treatment of virus diseases and new targeted oligonucleotides have been developed for different purposes. The click chemistry allowing the tolerated union between units with a wide diversity of functional groups represents a robust means of designing new hybrid compounds with an extraordinary diversity of applications. This review provides an overview of the most recent works related to the use of click chemistry methodology in the field of nucleosides, nucleotides and nucleic acids for pharmacological applications.

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Enhanced electrical conductivity of Au–LaNiO3 nanocomposite thin films by chemical solution deposition

RSC Advances ◽

10.1039/c5ra15152j ◽

2015 ◽

Vol 5 (94) ◽

pp. 76783-76787 ◽

Cited By ~ 10

Author(s):

H. L. Wang ◽

X. K. Ning ◽

Z. J. Wang

Keyword(s):

Thin Films ◽

Electrical Conductivity ◽

Electrical Properties ◽

Chemical Solution Deposition ◽

Nanocomposite Films ◽

Chemical Solution ◽

Solution Deposition ◽

Nanocomposite Thin Films ◽

One Step

Au–LaNiO3 (Au–LNO) nanocomposite films with 3.84 at% Au were firstly fabricated by one-step chemical solution deposition (CSD), and their electrical properties were investigated.

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Rapid electrochemical detection of coronavirus SARS-CoV-2

Nature Communications ◽

10.1038/s41467-021-21121-7 ◽

2021 ◽

Vol 12 (1) ◽

Cited By ~ 2

Author(s):

Thanyarat Chaibun ◽

Jiratchaya Puenpa ◽

Tatchanun Ngamdee ◽

Nimaradee Boonapatcharoen ◽

Pornpat Athamanolap ◽

...

Keyword(s):

Electrochemical Biosensor ◽

Rolling Circle Amplification ◽

Clinical Samples ◽

Rolling Circle ◽

Qrt Pcr ◽

Reverse Transcription Pcr ◽

Sandwich Hybridization ◽

Redox Active ◽

One Step ◽

The One

AbstractCoronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

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Thermal and Electrical Properties of Nucleic Acids and Proteins

Nature ◽

10.1038/188405a0 ◽

1960 ◽

Vol 188 (4748) ◽

pp. 405-406 ◽

Cited By ~ 58

Author(s):

J. DUCHESNE ◽

J. DEPIREUX ◽

A. BERTINCHAMPS ◽

N. CORNET ◽

J. M. VAN DER KAA

Keyword(s):

Electrical Properties ◽

Nucleic Acids ◽

Thermal And Electrical Properties

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Ligands with poly-fluorophenyl moieties promote a local structural rearrangement in the Spinach2 and Broccoli aptamers that increases ligand affinities

10.1101/2021.10.05.463302 ◽

2021 ◽

Author(s):

Sharif Anisuzzaman ◽

Ivan M Geraskin ◽

Muslum Ilgu ◽

Lee Bendickson ◽

George A Kraus ◽

...

Keyword(s):

Nucleic Acids ◽

Ligand Binding ◽

Small Molecule ◽

Binding Pocket ◽

Structural Rearrangement ◽

Structural Reorganization ◽

Ligand Binding Pocket ◽

Rna Remodeling ◽

Small Molecule Ligands ◽

Target Molecules

The interaction of nucleic acids with their molecular targets often involves structural reorganization that may traverse a complex folding landscape. With the more recent recognition that many RNAs, both coding and noncoding, may regulate cellular activities by interacting with target molecules, it becomes increasingly important to understand the means by which nucleic acids interact with their targets and how drugs might be developed that can influence critical folding transitions. We have extensively investigated the interaction of the Spinach2 and Broccoli aptamers with a library of small molecule ligands modified by various extensions from the imido nitrogen of DFHBI (3,5-difluoro-4-hydroxybenzylidene imidazolinone) that reach out from the Spinach2 ligand binding pocket. Studies of the interaction of these compounds with the aptamers revealed that poly-fluorophenyl-modified ligands initiate a slow change in aptamer affinity that takes an extended time (half-life of ~40 min) to achieve. The change in affinity appears to involve an initial disruption of the entrance to the ligand binding pocket followed by a gradual lockdown for which the most likely driving force is an interaction of the gateway adenine with a nearby 2'OH group. These results suggest that poly-fluorophenyl modifications might increase the ability of small molecule drugs to disrupt local structure and promote RNA remodeling.

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