scholarly journals Meningeal involvement of T-cell lymphoma. The problem in judging immunohistochemical staining for cerebrospinal fluid cytology.

1995 ◽  
Vol 34 (3) ◽  
pp. 585-586
Author(s):  
Mamoru MOCHIZUKI ◽  
Kyoko YOSHIDA ◽  
Michiko HIRUTA ◽  
Kikuo MORI ◽  
Shigeyuki ASANO
Keyword(s):  
Cerebrospinal Fluid ◽  
T Cell ◽  
Immunohistochemical Staining ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Cerebrospinal Fluid Cytology ◽  
Meningeal Involvement ◽  
Fluid Cytology

Adrenal extranodal NK/T-cell lymphoma diagnosed by fine-needle aspiration and cerebrospinal fluid cytology and immunophenotyping: A case report

2009 ◽  
Vol 37 (9) ◽  
pp. 686-695 ◽  
Author(s):  
Karla K. Dunning ◽  
Kitsada Wudhikarn ◽  
Anthony-Osei Safo ◽  
Carol J. Holman ◽  
Robert W. McKenna ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Case Report ◽  
T Cell ◽  
Fine Needle Aspiration ◽  
Cell Lymphoma ◽  
Needle Aspiration ◽  
T Cell Lymphoma ◽  
Fine Needle ◽  
Cerebrospinal Fluid Cytology ◽  
Fluid Cytology

scholarly journals Utility of a new notation to visualize flow cytometry analysis results: first preliminary comparison with immunohistochemistry to detect CD30 expression on T-cell lymphoma cells

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fumiyoshi Fujishima ◽  
Noriko Fukuhara ◽  
Hiroki Katsushima ◽  
Yasuhiro Nakamura ◽  
Hideo Harigae ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Immunohistochemical Staining ◽  
Cell Lymphoma ◽  
Large Cell ◽  
T Cell Lymphoma ◽  
Negative Rate ◽  
Miyagi Prefecture ◽  
Level I ◽  
A Minor

Abstract Background It is important to confirm CD30 expression in T-cell lymphoma cases, but immunohistochemical staining for CD30 is not commonly performed and no comparison has been done between the results of flow cytometry (FCM) and immunohistochemical staining for CD30. Therefore, we devised a notation that we termed proportion of immunoreactivity/expression for FCM (PRIME-F notation), based on the cellular proportion showing different antigen-antibody reactivity. Methods We retrospectively compiled 211 cases of T-cell lymphoma, assessed via FCM, from major hospitals in Miyagi Prefecture from January 2012 to January 2019, and compared 52 of these cases with the immunohistochemical immunoreactive (IR) pattern of CD30 (PRIME-I notation). The PRIME-F notation was divided into five levels: notations starting with “-” followed by 3, 2, and 1 “>” correspond to level-I, level-II, or level-III; notations starting with “(dim)+” correspond to level-IV; and those starting with “+” or “(bright)+” correspond to level-V. Results The 52 cases of PRIME-F notation with “+” included 16 cases of peripheral T-cell lymphoma (PTCL/NOS), 3 of follicular T-cell lymphoma (FTL), 3 of angioimmunoblastic T-cell lymphoma (AITL), 6 of extranodal NK/T-cell lymphoma/nasal type (ENKL), 18 of adult T-cell lymphoma (ATL), and 6 cases of anaplastic large cell lymphoma (ALCL). Eight of the 52 cases were immunohistochemically CD30-negative. In the PRIME-F level-I to III group (excluding false-positive cases), 21.7% (5 out of 23 cases) were < 10% positive for CD30 upon immunohistochemistry (IHC). Contrarily, in the level-IV & -V group, no CD30 positivity rate of < 10% upon IHC was found (0%) (p = 0.0497). In level-IV, 42.9% of cases presented a CD30 negative rate > 1/3 upon IHC, while in level-V, only 7.1% (one out of 14 cases) did. The CD30 negative rate tended to be low (p = 0.0877) in level-V. Conclusions To our knowledge, this is the first report describing the correspondence between FCM and immunohistochemistry findings for CD30 through newly proposed notations. The PRIME-F and PRIME-I notations for CD30 showed a minor positive correlation. The PRIME notation is considered universally applicable to antibodies, and notations of both FCM and IHC show great potential for big data.

scholarly journals T-cell Lymphoma Presenting Neutrophilic Inflammation in the Cerebrospinal Fluid

2020 ◽  
Vol 59 (4) ◽  
pp. 573-576
Author(s):  
Takaaki Nakamura ◽  
Yoshiki Takai ◽  
Kimihiko Kaneko ◽  
Hiroshi Kuroda ◽  
Tatsuro Misu ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
T Cell ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Neutrophilic Inflammation

scholarly journals Cerebrospinal fluid infiltration of primary cutaneous gamma delta T‐cell lymphoma

eJHaem ◽  
2021 ◽  
Author(s):  
Yoshikazu Hori ◽  
Yu Aruga ◽  
Chiaki Ikeda ◽  
Akiko Miyagi Maeshima ◽  
Koji Izutsu ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
T Cell ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Gamma Delta ◽  
Fluid Infiltration ◽  
Gamma Delta T Cell

Impact of Growth Factor Independence 1 in Human T-Cell Lymphomas; Pathogenic Potential Identified by Insertional Mutagenesis in a Murine T-Cell Lymphoma Model.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 5047-5047
Author(s):  
Magdalena Julia Dabrowska ◽  
Karen Dybkaer ◽  
Preben Johansen ◽  
Hans Erik Johnsen ◽  
Finn Skou Pedersen
Keyword(s):  
T Cell ◽  
Protein Expression ◽  
Posttranscriptional Regulation ◽  
Immunohistochemical Staining ◽  
Insertional Mutagenesis ◽  
Cell Lymphoma ◽  
Expression Patterns ◽  
T Cell Lymphoma ◽  
T Cell Lymphomas ◽  
Human T Cell

Abstract Abstract 5047 Introduction The transcriptional repressor and oncogene Growth factor independence 1 (Gfi1) has a major oncogenic potential and is aberrantly expressed in murine lymphomas and several human cancers. Gfi1 is a key regulator of stem cell quiescence and plays a significant role in T-cell development, lineage commitment, and influences development of maturate granulocytes and monocytes. The genomic locus on murine chromosome 5 encoding Gfi1 is a frequent integration locus activated in T-cell lymphomas induced by the SL3-3 Murine Leukemia Virus (MLV) as well as other MLVs, indicating that Gfi1 is essential in development of these tumors. In the SL3-3 induced T-cell lymphoma model, retroviral insertions in the Gfi1 3'UTR have been demonstrated to decouple microRNA-mediated posttranscriptional regulation of protein expression (Dabrowska et al, 2009) further supporting its role in lymphomagenesis. In human cancers, Gfi1 protein expression has been observed in HTLV-1 induced ATLL and SCLC but no knowledge exists on how Gfi1 contributes to initiating and maintaining human T-cell lymphomas. Methods Gfi1 gene and protein expression patterns were determined in precursor and mature human T-cell lymphomas by real time PCR and Western blot analysis. Furthermore, localization and expression patterns of the Gfi1 protein was determined in the human T-cell lymphomas by immunohistochemical staining with Gfi1 antibodies and compared to similar staining of MLV induced tumors. Results Our results demonstrated that Gfi1 mRNA and protein levels vary significantly among the human T-cell lymphomas, and do not always show a direct proportional pattern. Thus, Gfi1 mRNA expression can be relatively high without resulting in a corresponding high protein expression, suggesting that a microRNA mediated posttranscriptional regulation exists in some tumors but may be disrupted in others. Furthermore, an additional Gfi1 protein variant was identified in one of the T-cell lymphoma entities. Immunohistochemical staining demonstrated varying Gfi1 protein expression in both nucleus and cytoplasm in the T-cell lymphomas and different distributions of the protein within the tumor and tumor cells were observed among samples. Staining of normal human tonsil demonstrated Gfi1 protein to be localized in the cytoplasm. We hypothesise that regulation of Gfi1 may include shuttling between cytoplasm and nucleus and that lymphomagenesis enables unlimited nuclear access. Conclusion Our data shows that deregulated Gfi1 expression plays a major role in the development of MLV induced lymphomas and strongly indicates that retroviral insertional mutagenesis in murine models of human NHLs can be used to identify new genes involved in lymphomagenesis and, by use of functional assays, their impact on human lymphomas can be evaluated. Disclosures No relevant conflicts of interest to declare.

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