scholarly journals In Vitro Cultivation of Dental Pulp Stem Cells from Human Exfoliated Deciduous Teeth in Low-Xenogeneic-Serum Containing Media

2016 ◽  
Vol 116 (1) ◽  
pp. 3-11
Author(s):  
T. Kleplová ◽  
K. Z. Browne ◽  
T. Soukup ◽  
J. Suchánek
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Deciduous Teeth ◽  
In Vitro Cultivation ◽  
Human Exfoliated Deciduous Teeth

scholarly journals Análise bibliométrica do uso de células-tronco em pesquisas odontológicas

2020 ◽  
Vol 8 (12) ◽  
Author(s):  
Jéssica Gomes Alcoforado de Melo ◽  
Diego Moura Soares
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
Dental Pulp Stem Cells ◽  
Deciduous Teeth ◽  
Level Laser ◽  
Human Dental Pulp ◽  
Low Level Laser Irradiation ◽  
Human Exfoliated Deciduous Teeth

As pesquisas com células-tronco, seja de origem dental ou não, vêm crescendo na área da odontologia nos últimos anos em decorrência das possibilidades terapêuticas que a utilização desse tipo celular oferece. Este estudo visa demonstrar um panorama brasileiro das pesquisas com células-tronco realizadas no país por pesquisadores da área da odontologia nos anos de 2014 até 2018, com base nos anais de trabalhos apresentados nas Reuniões Anuais da Sociedade Brasileira de Pesquisa Odontológica (SBPqO). Foi analisado aspectos como tipo de instituição, se as pesquisas foram financiadas e qual a agencia de fomento, tipo de estudo, estado e região que desenvolveu a pesquisa, tipo de célula e fonte da célula-tronco utilizada. Foram analisados um total de 15,214 resumos, deste total 96 estudos foram incluídos por se enquadrarem com os critérios de inclusão. A região Sudeste foi responsável por 65,7% dessa produção. As pesquisas realizadas nas instituições estaduais representaram 42,7% da produção nacional e 59,4% dos trabalhos foram financiados. As células-tronco humanas foram o tipo mais utiilizado, especialmente as originadas da polpa dentária (25%). Conclui-se que há uma escassez da produção científica voltada para as células-tronco na odontologia, bem como a necessidade de descentralização dessa produção nas demais regiões brasileiras.Descritores: Células-Tronco; Odontologia; Pesquisa em Odontologia.ReferênciasGronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA.2000;97(25):13625-630.Freshney IR, Stacey GN, Aurebach JM. Culture of human stem cells: culture of specialized cells. New York: Wisley-Liss; 2007.Serakinci N, Keith WN. Therapeutic potential of adult stem cells. Eur J Cancer.2006;42(9):1243-46.Bianco P, Riminucci M, Gronthos S, Robey PG. Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells.. 2001;19(3):180-92.Mvula B, Mathope T, Moore T, Abrahamse H. The effect of low-level laser irradiation on adult human adipose-derived stem cells. Lasers Med Sci. 2008;23(3):277-82.Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-301.Slack JM. Stem cell in epithelial tissue. Science. 2000; 287:1431-33.Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG et al. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA.2003; 100(10):5807-12.Chen SC, Marino V, Gronthos S, Bartold PM. Location of putative stem cells in human periodontal ligament. J Periodontal Res. 2006; 41(6):547-53.Nuti N, Corallo C, Chan BMF, Ferrari M, Gerami-Naini B. Multipotent differentiation of human dental pulp stem cells: a literature review. Stem Cell Rev. 2016;12(5):511-523.Barboza CAG, Ginani F, Soares DM, Henrique ACG, Freitas RA. Low-level laser irradiation induces in vitro proliferation of mesenchymal stem cells. Einstein (São Paulo) 2014;12(1):75-81.Soares DM, Ginani F, Henriques AG, Barboza CAG. Effects of laser therapy on the proliferation of human periodontal ligament stem cells. Lasers Med Sci. 2015;30(3):1171-74.Ginani F, Soares DM, Rabêlo LM, Rocha HAO, Souza LB, Barboza CAG. Effect of a cryopreservation protocol on the proliferation of stem cells from human exfoliated deciduous teeth. Acta Odontol Scand. 2016;74(8):598-604.Maciel MMSA, Silva KBN, Melo JGA, Soares DM. Metodologia ativa aplicada ao ensino odontológico: um panorama nacional a partir de um estudo bibliométrico. Arch Health Invest. 2019;8(2):74-78.Melo NB, Fernandes Neto JA, Catão MHCV, Bento PM. Metodologia da Problematização e Aprendizagem Baseada em Problemas na Odontologia: análise bibliométrica dos trabalhos apresentados nas Reuniões da SBPqO. Revista da ABENO 2017;17(2):60-7.Xavier AFC, Silva ALO, Cavalcanti AL. Análise da produção científica em Odontologia no nordeste brasileiro com base em um congresso odontológico. Arq Odontol.2011;47(3):127-34.Aquino SN, Martelli DR, Bonan PRF, Laranjeira AL, Martelli Júnior H. Produção  científica odontológica e relação com agências de financiamento de pesquisa. Arq Odontol. 2009; 45(3):142-46.Pontes KT, Silva EL, Macedo Filho RA, Silva DR, Lima FJ. Estudo bilbiométrico da produção científica em endodontia. Arch Health Invest. 2017;6(9):435-38.Soares DM, Maciel MMSA, Figueiredo-Filho A, Melo JGA. Brazilian scientific production in periodontics: a national panorama from a bibliometric study. Rev Clin Periodoncia Implantol Rehabil Oral. 2019;12(2):66-9.Taumaturgo VM, Vasques EFL, Figueiredo VMG. A Importância Da  Odontologia Nas Pesquisas  Em Células-Tronco. Rev Bahiana Odontol. 2016;7(2):166-71.Primo BT, Grazziotin-Soares R, Bertuzzi D, Claudy MP, Hernandez PAG, Fontanella VRC. Produção científica da ULBRA: análise do número e do delineamento das pesquisas publicadas nos suplementos da Brazilian Oral Research (SBPqO). Stomatos. 2010;16(31):69-76.Zorzanelli RT, Speroni AV, Menezes RA, Leibing A. Pesquisa com células-tronco no Brasil: a produção de um novo campo científico. Hist ciênc saúde-Manguinhos 2017;24(1):129-44.Lan X, Sun Z, Chu C, Boltze J, Li S. Dental Pulp Stem Cells: An Attractive Alternative for Cell Therapy in Ischemic Stroke. Front Neurol. 2019;10:824.Aydin S, Sahin F. Stem cells derived from dental tissues. Adv Exp Med Biol. 2019;1144:123-32.

Stem Cells Derived from Human Exfoliated Deciduous teeth [shed] in Neuronal Disorders: A Review

2020 ◽  
Vol 16 ◽  
Author(s):  
Minu Anoop ◽  
Indrani Datta
Keyword(s):  
Stem Cells ◽  
Neurodegenerative Diseases ◽  
Dental Pulp ◽  
Therapeutic Potential ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Proliferative Potential ◽  
Pulp Tissue ◽  
Human Exfoliated Deciduous Teeth

: Most conventional treatments for neurodegenerative diseases fail due to their focus on neuroprotection rather than neurorestoration. Stem cell‐based therapies are becoming a potential treatment option for neurodegenerative diseases as they can home in, engraft, differentiate and produce factors for CNS recovery. Stem cells derived from human dental pulp tissue differ from other sources of mesenchymal stem cells due to their embryonic neural crest origin and neurotrophic property. These include both dental pulp stem cells [DPSCs] from dental pulp tissues of human permanent teeth and stem cells from human exfoliated deciduous teeth [SHED]. SHED offer many advantages over other types of MSCs such as good proliferative potential, minimal invasive procurement, neuronal differentiation and neurotrophic capacity, and negligible ethical concerns. The therapeutic potential of SHED is attributed to the paracrine action of extracellularly released secreted factors, specifically the secretome, of which exosomes is a key component. SHED and its conditioned media can be effective in neurodegeneration through multiple mechanisms, including cell replacement, paracrine effects, angiogenesis, synaptogenesis, immunomodulation, and apoptosis inhibition, and SHED exosomes offer an ideal refined bed-to-bench formulation in neurodegenerative disorders. However, in spite of these advantages, there are still some limitations of SHED exosome therapy, such as the effectiveness of long-term storage of SHED and their exosomes, the development of a robust GMP-grade manufacturing protocol, optimization of the route of administration, and evaluation of the efficacy and safety in humans. In this review, we have addressed the isolation, collection and properties of SHED along with its therapeutic potential on in vitro and in vivo neuronal disorder models as evident from the published literature.

scholarly journals Isolation and culture of dental pulp stem cells from permanent and deciduous teeth

2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Shagufta Naz ◽  
Farhan Raza Khan ◽  
Raheela Rahmat Zohra ◽  
Sahreena Salim Lakhundi ◽  
Mehwish Sagheer Khan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Tooth Pulp ◽  
Human Teeth ◽  
Creative Commons ◽  
Calcified Tissue

Objective: To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent and deciduous teeth. Methods: It was an in-vitro experimental study. Human teeth were collected from 13 apparently healthy subjects including nine adults and four children. After decoronation dental pulps were extirpated from teeth and cultured via explant method in a stem cell defined media. Data was analyzed by descriptive statistics. Results: As above MSCs emerged exhibiting fibroblast-like morphology. In vitro culture was positive for 100% (9/9) and 75% (3/4) of the permanent and deciduous teeth respectively. First cell appeared from deciduous teeth pulp in 10±6.2 days while permanent teeth pulp took 12.4±3.7 days. Together, 26.6±3.6 and 24.5±3.5 days were required for permanent and deciduous tooth pulp stem cells to be ready for further assays. Conclusions: The protocol we developed is easy and consistent and can be used to generate reliable source of MScs for engineering of calcified and non-calcified tissue for regenerative medicine approaches. doi: https://doi.org/10.12669/pjms.35.4.540 How to cite this:Naz S, Khan FR, Zohra RR, Lakhundi SS, Khan MS, Mohammed N, et al. Isolation and culture of dental pulp stem cells from permanent and deciduous teeth. Pak J Med Sci. 2019;35(4):---------. doi: https://doi.org/10.12669/pjms.35.4.540 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals Present Status of Research on the Regenerative Potential of Dental Pulp Stem Cells in Malaysia: A Systematic Review

2021 ◽  
Vol 8 (1) ◽  
pp. 304-309
Author(s):  
Nazmul Haque
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Present Status ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Invasive Technique ◽  
Human Exfoliated Deciduous Teeth

Stem cells from human exfoliated deciduous teeth (SHED) or dental pulp stem cells (DPSCs) from permanent teeth are considered promising sources of mesenchymal stem cells. It requires a less invasive technique to isolate stem cells from exfoliated or permanent teeth. Hence this study aimed to identify the present status of research on the regenerative potential of SHED/DPSCs in Malaysia. The results indicate that only 60 articles were published in regenerative medicine from Malaysia till 5th July 2019. Only 16 tertiary institutes and four industries/clinics were involved in these studies. A poor pattern of collaboration has also been identified. Outcomes of this study have emphasized the conduction of more research on the regenerative potential of SHED/DPSCs, and active collaboration among the tertiary institutes and industries for successful translation of these cells from bench side to bedside.

scholarly journals VEGFR1 primes a unique cohort of dental pulp stem cells for vasculogenic differentiation

2021 ◽  
Vol 41 ◽  
pp. 332-344
Author(s):  
MT Bergamo ◽  
◽  
Z Zhang ◽  
TM Oliveira ◽  
JE Nör
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Vascular Endothelial Cells ◽  
Dental Pulp Stem Cells ◽  
Deciduous Teeth ◽  
Microvascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Pulp Tissue ◽  
Immunodeficient Mice

Dental pulp stem cells (DPSCs) constitute a unique group of cells endowed with multipotency, self-renewal, and capacity to regenerate the dental pulp tissue. While much has been learned about these cells in recent years, it is still unclear if each DPSC is multipotent or if unique sub-populations of DPSCs are “primed” to undergo specific differentiation paths. The purpose of the present study was to define whether a sub-population of DPSCs was uniquely primed to undergo vasculogenic differentiation. Permanent-tooth DPSCs or stem cells from human exfoliated deciduous teeth (SHED) were flow-sorted for vascular endothelial growth factor receptor 1 (VEGFR1) and exposed to vasculogenic differentiation medium, i.e., Microvascular-Endothelial-Cell-Growth-Medium-2-BulletKit™ supplemented with 50 ng/mL rhVEGF165 in the presence of 0 or 25 μg/mL anti-human VEGF antibody (bevacizumab; Genentech). In addition, sorted SHED (i.e., VEGFR1high or VEGFR1low) were seeded in biodegradable scaffolds and transplanted into the subcutaneous space of immunodeficient mice. Despite proliferating at a similar rate, VEGFR1high generated more in vitro sprouts than VEGFR1low cells (p < 0.05). Blockade of VEGF signaling with bevacizumab inhibited VEGFR1high-derived sprouts, demonstrating specificity of responses. Similarly, VEGFR1high SHED generated more blood vessels when transplanted into murine hosts than VEGFR1low cells (p < 0.05). Collectively, these data demonstrated that DPSCs contain a unique sub-population of cells defined by high VEGFR1 expression that are primed to differentiate into vascular endothelial cells. These data raise the possibility of purifying stem cells with high vasculogenic potential for regeneration of vascularized tissues or for vascular engineering in the treatment of ischemic conditions.

scholarly journals Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs) and Dental Pulp Stem Cells (DPSCs) Display a Similar Profile with Pericytes

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Shao Yue Zhu ◽  
Chang Yong Yuan ◽  
Yi Fan Lin ◽  
Hao Liu ◽  
Yan Qi Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Mrna Expression ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Deciduous Teeth ◽  
Potential Candidate ◽  
Migration Ability ◽  
Significant Difference ◽  
Human Exfoliated Deciduous Teeth

Background. Pericytes play an important role in forming functional blood vessels and establishing stable and effective microcirculation, which is crucial for vascular tissue engineering. The slow ex vivo expansion rate, limited proliferative capacity, and variability of tissue-specific phenotypes would hinder experimental studies and clinical translation of primary pericytes. In this study, the angiogenic and pericyte functions of stem cells from human exfoliated deciduous teeth (SHEDs) and postnatal human dental pulp stem cells (DPSCs) were investigated. Methods. Osteogenic and adipogenic induction assays were performed to evaluate the mesenchymal potential of SHEDs, DPSCs, and pericytes. An in vitro Matrigel angiogenesis assay was conducted to reveal the ability of SHEDs, DPSCs, and pericytes to stabilize vascular-like structures. Quantitative real-time polymerase chain reaction (RT-qPCR) was performed to evaluate mRNA expression. Flow cytometry, western blotting, and immunostaining were used to assess the protein expression. Wound healing and transwell assays were performed to evaluate the migration ability of SHEDs, DPSCs, and pericytes. Results. The osteogenic and adipogenic induction assays showed that SHEDs, DPSCs, and pericytes exhibited similar stem cell characteristics. The mRNA expression levels of PDGFR-β, α-SMA, NG2, and DEMSIN in SHEDs and DPSCs cultured in EC medium were significantly higher than those in the control groups on day 7 ( P < 0.05 ), but significantly higher than those in the pericytes group on day 14 ( P < 0.05 ). Flow cytometry showed that high proportions of SHEDs and DPSCs were positive for various pericyte markers on day 7. The DPSCs, SHEDs, and pericytes displayed strong migration ability; however, there was no significant difference among the groups ( P > 0.05 ). Conclusion. The SHEDs and DPSCs display a profile similar to that of pericytes. Our study lays a solid theoretical foundation for the clinical use of dental pulp stem cells as a potential candidate to replace pericytes.

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