Umbilical Cord Blood Acidosis in Term Pregnancies With Gestational Diabetes Mellitus and Its Relations to Maternal Factors and Neonatal Outcomes

Background: The in utero environment has many factors that can support cell differentiation. Cytokines, chemokines and growth factors play big roles in haematopoietic mechanisms. Some diseases like gestational diabetes mellitus (GDM) might affect the environment and haematopoietic stem cell (HSC) quality. The aim of this study is to investigate the adverse effects of GDM on umbilical cord blood (UCB) HSC in terms of differentiation potency including the UCB parameters used for banking and transplantation purposes.Methods: UCB-HSC was collected from 42 GDM and 38 normal pregnancies. UCB-HSC was isolated and further enriched using immuno-magnetic separation beads (MACS). The UCB-HSC were cultured in methylcellulose media to investigate the differentiation potency. The level of erythropoietin (EPO) and insulin in the UCB plasma was measured using enzyme linked immunoassay (ELISA) technique.Result: The UCB parameters; volume, total nucleated count (TNC) and total CD34+ cells were significantly reduced in the GDM group compared to the control group. The number of HSC progenitors' colonies were significantly reduced in the GDM group except for progenitor BFU-E, which was significantly increased (GDM = 94.19 ± 6.21, Control = 73.61 ± 2.73, p = 0.010). This data was associated with higher EPO level in GDM group. However, the insulin level in the GDM group was comparable to the Control group.Conclusion: Our results suggest that the changes in the in utero environment due to abnormalities during pregnancy such as GDM might affect the differentiation potency of UCB-HSC. These findings can be considered as an additional parameter for the inclusion and exclusion criteria for UCB banking, particularly for mothers with GDM.

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Gestational diabetes mellitus in pregnancy increased erythropoietin level affecting differentiation potency of haematopoietic stem cell of umbilical cord blood

10.21203/rs.3.rs-40849/v2 ◽

2020 ◽

Author(s):

Mohd Razif Mohd Idris ◽

Fazlina Nordin ◽

Zaleha Abdullah Mahdy ◽

S Fadilah Abd Wahid

Keyword(s):

Diabetes Mellitus ◽

Stem Cell ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

In Utero ◽

Haematopoietic Stem Cell ◽

Control Group

Abstract Background The in utero environment has many factors that can support cell differentiation. Cytokines, chemokines, and growth factors play big roles in haematopoietic mechanisms. Some diseases like gestational diabetes mellitus (GDM) might affect the environment and quality of haematopoietic stem cell (HSC). This study aims to investigate the adverse effects of GDM on umbilical cord blood (UCB) HSC regarding its differentiation potency, including the UCB parameters used for banking and transplantation purposes. Methods UCB-HSC was collected from 42 GDM and 38 normal pregnancies. UCB-HSC was isolated and further enriched using immuno-magnetic separation beads (MACS). The UCB-HSC were cultured in methylcellulose media to investigate the differentiation potency. The level of erythropoietin (EPO) and insulin in the UCB plasma was measured using enzyme-linked immunoassay (ELISA) technique. Results The UCB parameters, i.e., volume, total nucleated count (TNC), and total CD34+ cells, were significantly reduced in the GDM group compared to the control group. The number of HSC progenitor colonies was significantly reduced in the GDM group, except for progenitor BFU-E, which was significantly increased (GDM = 94.19 ± 6.21, Control = 73.61 ± 2.73, p = 0.010). This data was associated with a higher EPO level in the GDM group. However, the insulin level in the GDM group was comparable to the control group. Conclusion Our results suggest that the changes in the in utero environment due to abnormalities during pregnancy, such as GDM might affect the differentiation potency of UCB-HSC. These findings can be considered as an additional parameter for the inclusion and exclusion criteria for UCB banking, particularly for mothers with GDM.

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Differential mRNA and Long Noncoding RNA Expression Profiles in Umbilical Cord Blood Exosomes from Gestational Diabetes Mellitus Patients

DNA and Cell Biology ◽

10.1089/dna.2020.5783 ◽

2020 ◽

Vol 39 (11) ◽

pp. 2005-2016

Author(s):

Minkai Cao ◽

Le Zhang ◽

Yu Lin ◽

Zhengying Li ◽

Jianjuan Xu ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Expression Profiles ◽

Rna Expression

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Maternal, placental and cordonal metallomic profiles in gestational diabetes mellitus

Metallomics ◽

10.1039/c8mt00331a ◽

2019 ◽

Vol 11 (3) ◽

pp. 676-685 ◽

Cited By ~ 6

Author(s):

Marco Roverso ◽

Valerio Di Marco ◽

Denis Badocco ◽

Paolo Pastore ◽

Marilia Calanducci ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Diabetic Mothers

The concentration of several elements changes in umbilical cord blood of fetuses from diabetic mothers and from controls.

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Circular RNA expression profiles in umbilical cord blood exosomes from normal and gestational diabetes mellitus patients

Bioscience Reports ◽

10.1042/bsr20201946 ◽

2020 ◽

Vol 40 (11) ◽

Author(s):

Minkai Cao ◽

Le Zhang ◽

Yu Lin ◽

Zhengying Li ◽

Jianjuan Xu ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Expression Profiles ◽

Circular Rna ◽

Cellular Functions ◽

Pathway Analyses

Abstract Circular RNA (circRNA) is a novel member of endogenous noncoding RNAs with widespread distribution and diverse cellular functions. Recently, circRNAs have been identified for their enrichment and stability in exosomes. However, the roles of circRNAs from umbilical cord blood exosomes in gestational diabetes mellitus (GDM) occurrence and fetus growth remains poorly understood. In the present study, we used microarray technology to construct a comparative circRNA profiling of umbilical cord blood exosomes between GDM patients and controls. We found the exosome particle size was larger, and the exosome concentration was higher in the GDM patients. A total of 88,371 circRNAs in umbilical cord blood exosomes from two groups were evaluated. Of these, 229 circRNAs were significantly up-regulated and 278 circRNAs were significantly down-regulated in the GDM patients. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses demonstrated that circRNA parental genes involved in the regulation of metabolic process, growth and development were significantly enriched, which are important in GDM development and fetus growth. Further circRNA/miRNA interactions analysis showed that most of the exosomal circRNAs harbored miRNA binding sites, and some miRNAs were associated with GDM. Collectively, these results lay a foundation for extensive studies on the role of exosomal circRNAs in GDM development and fetus growth.

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