scholarly journals Effects of temperature, light conditions and gibberellic acid on the in vitro germination of Protea cynaroides L. embryos

2010 ◽  
Vol 9 (47) ◽  
pp. 8032-8037 ◽  
Author(s):  
C Wu H ◽  
S du Toit E
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Germination ◽  
Light Conditions ◽  
Effects Of Temperature

Control of browning during seed germination of Meizotropis pellita: a rare and an endangered plant

2021 ◽  
Vol 16 (8) ◽  
pp. 118-120
Author(s):  
Renu Chandola ◽  
Anshulika Upadhyaya
Keyword(s):  
Seed Germination ◽  
Culture Media ◽  
Light Condition ◽  
Endangered Plant ◽  
In Vitro Germination ◽  
Light Conditions ◽  
Low Intensity ◽  
Browning Reaction ◽  
Threatened Plant Species

Meizotropis pellita is a rare, endangered and threatened plant species found in Patwadanger forest. In nature this species grows from rootstock in precise and sensitive habitats. However, phenolic compounds provoke browning reaction which is responsible for lethal browning in plant tissue culture and hindering in vitro germination. The aim of this study was to minimize the effect of phenolic compound during seed germination. We evaluate different antibrowning treatments in Meizotropis pellita seeds. The seeds were pretreated in Polyvinylpyrrolidone and then treated seeds were cultured on MS media and sterilized seeds were directly inoculated on PVP containing MS media. These culture media incubate under dark and light conditions at different durations respectively. Our results showed that addition of antibrowning agent, PVP inhibit onset of browning. Dark and light condition also played a crucial role on onset of browning. Delayed and low intensity of browning were observed in case of seeds that were inoculated on MS media containing PVP and were kept in dark. The present research offers a positive in vitro seed germination protocol for Meizotropis pellita conservation.

In vitro growth of some species of Ascochyta Lib.

2012 ◽  
Vol 7 (6) ◽  
pp. 1076-1083
Author(s):  
Tomasz Kosiada
Keyword(s):  
High Temperatures ◽  
Low Temperatures ◽  
Mycelial Growth ◽  
Potato Dextrose Agar ◽  
In Vitro Growth ◽  
Light Conditions ◽  
Oatmeal Agar ◽  
The Family ◽  
Effects Of Temperature

AbstractFungi from the genus Ascochyta are generally facultative saprotrophs, which cause diseases in both monocots and dicots. Over 1 000 species belonging to this genus have been identified, 18 of which infect monocot plants from the family Poaceae. This study analyses the effects of temperature and light on the growth of selected fungi which infect monocots (A. agrostidis, A. avenae, A. brachypodii, A. desmazieri, A. digraphidis, A. ducis-aprutii, A. festucae, A. graminea, A. hordei, A. hordei var. americana, A. hordei var. europea, A. hordei var. hordei, A. melicae, A. phleina, A. skagwayensis, A. sorghi, A. stipae, A. zeicola), grown on three types of media; Potato Dextrose Agar (PDA), Coon’s agar (CN) and oatmeal agar (OMA). The fastest growth among the analyzed fungi at low temperatures was found in Ascochyta melicae, while at high temperatures it was A. zeicola. The fastest in vitro growth (average of all fungi) was observed on CN medium at 20°C (3.4 mm/day), while the lowest on OM medium at 5°C (1.0 mm/day). Radial mycelial growth in dark and the light conditions varied. On average, all isolates grew faster in the dark (3.1 mm/day) than in the light (1.9 mm/day). The greatest effect on the production of pycnidia was found for the isolates. Variation in growth and production of pycnidia depended on temperature, medium and lighting for fungi from the genus Ascochyta infecting monocots. Such variation indicates a potential occurrence of these fungi in different environments.

scholarly journals Micropropagation of Satureja obovata Lag.

HortScience ◽  
1997 ◽  
Vol 32 (7) ◽  
pp. 1278-1280 ◽  
Author(s):  
M.L. Arrebola ◽  
O. Socorro ◽  
A. Barceló-Muñoz ◽  
E. Simón-Pérez ◽  
Fernando Pliego-Alfaro
Keyword(s):  
Gibberellic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
In Vitro Germination ◽  
Free Medium ◽  
Phase Chemical

A micropropagation procedure for juvenile and adult savory (Satureja obovata Lag.) explants is described. Pretreatment of the nutlets with gibberellic acid (0.57 mm) did not improve in vitro germination. Optimum shoot proliferation of juvenile and adult material was obtained on medium containing 2.22 μm N6-benzyladenine. Rooting and acclimatization of juvenile shoots were accomplished in vivo, while adult shoots were rooted in vitro after 3 days of exposure to 4.92 μm indole-3-butyric acid followed by subsequent transfer to auxin-free medium. More than 95% survival of adult rooted plants was obtained during the acclimatization phase. Chemical names used: gibberellic acid (GA3); N6-benzyladenine (BA); indole-3-butyric acid (IBA); isopentenyladenine (2iP).

scholarly journals Seed origin, storage conditions, and gibberellic acid on in vitro germination of Campomanesia adamantium (Cambess.) O. Berg

2016 ◽  
Vol 15 (32) ◽  
pp. 1731-1737
Author(s):  
Roberta Damiani Claudia ◽  
Darc da Silva Leandro ◽  
Goelzer Ademir ◽  
Gatti Deo Thamiris
Keyword(s):  
Gibberellic Acid ◽  
Storage Conditions ◽  
In Vitro Germination ◽  
Seed Origin

scholarly journals In vitro Conservation of Campanula sclerophylla Kolak ? Endemic Endangered Species of Western Caucasus

2016 ◽  
Vol 26 (2) ◽  
pp. 143-149 ◽  
Author(s):  
TM Kolomiets ◽  
VI Malyarovskaya ◽  
LS Samarina
Keyword(s):  
Light Intensity ◽  
Shoot Growth ◽  
Slow Growth ◽  
Shoot Length ◽  
Multiplication Rate ◽  
Light Conditions ◽  
Western Caucasus ◽  
Half Strength ◽  
Effects Of Temperature

The study was conducted to develop micropropagation and slow?growth conservation protocol for endangered relict plant species Campanula sclerophylla. The best multiplication rate up to 7.2 shoots per explant was obtained on half strength MS supplemented with 3 mg/l BAP and 1 mg/l IAA. The effects of temperature/light conditions, sorbitol and ABA on shoot growth of Campanula sclerophylla were studied. Addition of 3 g/l sorbitol and 5 mg/l ABA inhibited shoot length and multiplication of C. sclerophylla. Explants were stored for 9 months without subculture at 7º? and light intensity of 23 lux.Plant Tissue Cult. & Biotech. 26(2): 143-149, 2016 (December)

scholarly journals Promotion of Immature Seed Germination in Jacaranda mimosifolia

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1485-1486
Author(s):  
Ikuo Miyajima ◽  
Adriana Kato ◽  
Juan Carlos Hagiwara ◽  
Diego Mata ◽  
Gabriela Facciuto ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Stage ◽  
Immature Seed ◽  
In Vitro Germination ◽  
Immature Seeds ◽  
Mature Seeds ◽  
Jacaranda Mimosifolia

In vitro germination of immature seeds of Jacaranda mimosifolia treated with gibberellic acid (GA3) was studied. Immature seeds were collected monthly after crossings and sown on Murashige and Skoog (1962) medium with 3.0% sucrose and 0.6% agar after soaked 24 hours with 0, 10, 100, and 500 mg·L–1 GA3 solutions. Though germination was observed in the immature seeds harvested 2 months after crossing (2 MAC), the rate was quite low. When immature seeds of 3 MAC treated with 100 or 500 mg·L–1 GA3 solution were cultured, >60% germination were obtained within 2 weeks after culturing. These results indicate that immature seeds of 3 MAC treated with adequate GA3 solutions, seedlings can be obtained precociously and the period from crossing to the seedling stage was shorter than for mature seeds.

scholarly journals In Vitro Germination of Immature Prunus lusitanica Seed

HortScience ◽  
2017 ◽  
Vol 52 (8) ◽  
pp. 1122-1124 ◽  
Author(s):  
Justin A. Schulze ◽  
Jason D. Lattier ◽  
Ryan N. Contreras
Keyword(s):  
Tissue Culture ◽  
Gibberellic Acid ◽  
Cold Stratification ◽  
In Vitro Germination ◽  
Seed Conditioning ◽  
Shoot Emergence ◽  
Culture Protocol

A tissue culture protocol was developed to germinate immature Prunus lusitanica seeds in vitro. The study was conducted by first identifying the best media for germination, followed by investigating effects of seed conditioning. In Expt. I, seeds were collected 12 weeks after pollination (WAP) ± 1 week and placed on media after removing the pericarp. Eight different MS media (Murashige and Skoog, 1962) were tested (M1–M8) containing two concentrations each of 6-benzylaminopurine (BA), gibberellic acid (GA3), and sucrose. The longest shoots resulted from M4 (1.45 µm GA3, 6 µm BA, and 30 g·L−1 sucrose), followed by M1 (0 µm GA3, 3 µm BA, and 30 g·L−1 sucrose). Radicle and shoot emergence was greater than or equal to 90% for M1, M3, and M4 after a stratification treatment. In Expt. II, M1 was used to test root and shoot emergence at 6, 9, and 12 WAP, with and without cold stratification. Little success was seen 6 and 9 WAP, with only callus development in 6 WAP, nonstratified seed. Cold stratification increased shoot emergence in the 12 WAP group from 4% to 28%, appearing to be critical for shoot emergence. If the cotyledons are retained on the seed, future efforts to expedite breeding of P. lusitanica using in vitro germination should not be collected before 12 WAP and will benefit from cold stratification before germinating on M1 or M4. Chemical names: 6-benzylaminopurine (BA), gibberellic acid (GA3).

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